ISfinder Mirror Site

ISCaje5

Family IS1595
Group ISPna2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
ARWS01000006	ND	Campylobacter jejuni	Campylobacter jejuni 255 Campylobacter lari RM16712

DNA section

IS Length : 2899 bp

Ends

IR Length : 20/24

IRL : GGCTATGTTTTTATTAAGTATTGAAATTTAAGCTTTATTTTGCTAAAATA
IRR : GGCTCTGTTTTTATAGTGTATTGATTTCTAAAAAATGCTATCAATAGAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATAATTTAAT	AAAATTTT	TAGCATTAAA	8
GAGAGCATAT	TTTTAATT	TCATTTCCTA	8

DNA sequence

GGCTATGTTTTTATTAAGTATTGAAATTTAAGCTTTATTTTGCTAAAATATCTACATTAAAAATAGCACAATAAAGGCGGTAAAATGGCAACTTCTAACG
CAGAACTTGAAATCGTAAAACAGCTTTTTAGCACTCTAAGCGATGATGATAAAAAATCATTTCTAAAATCTATCAAAAACAAAGAAAAATCAGAGCAAAA
TTTAATACTCTCAAAAGAGATAAAAGCGTGTCCGCATTGCAAATCTACTAATTTTGTGAAAAATGGAAAAAAAGATGGCAAACAAAGATTTATGTGTATG
GATTGTCATAAGACATTTACTGTTACAAATAACTCAATCTTTTTTAAAACCCAAAAAGACCTTGAAATTTGGCAAAAATATATCCATTGTATGATTGAAA
AATACTCACTTAGAAAAACCGCTGAAATTTGTGGCATTGCACTATCTACTGCGTTTTTTTGGCGACATAAAATCTTAGACACATTGCAAAAAATGCAAGA
TGAAGTAAAACTTAATGGCATAGTAGAAGCAGATGAAACATTTATGCCTATCTCATATAAAGGTCATCACAAGGATTTTAACTTTCCACGCTTAGCAAAG
AAAAGAGGAACTGCAAACACTAAGCGTGGCTTATCAAAAGAACTTGTTTGCATACCTTGCGTAGTAAATTTAAATGGCAAATCAATAGCAAAAATATCTA
ATCTGGGTAAGCCAAATATTGCTAGTTTATCCAAAGTAATACAAGGCAAAGTTGAGAAAGAAAGCGTATTTGTAACTGATAGCCTACGCTCATATTTAAA
ACTATCAAATGAAATGAGCCTAAACCATATCCGCATACCACGAAACAAACATAGTAACGGCTCGTTTAATATTCAAGTCGTAAATAACTACCATAGCAGA
CTTAAATCAATGCTTGTGTATAATTTTAAAGGCGTGGCTACTAAGTATCTAAATAATTATCTTGTCTATCACAACTTTGTAAATTTTGCAAAAGAGAGTA
GAAAAGATAAAGAACAAATTTTGCTAAACCATATACTAAACACAAATTGCATAAGCCAAAGCATAAGCGTAGCAAATAGACCTGCCGTGCCGTTATTGGT
AGCGTAAGCTGTAAAAAATTTAAAAAAGGAATAAAATTGTCATATAAAAAATATTTAAACAATGTTACATTGGGTGATTGCTGTGAGCTAATGAAAAATA
TTCCTAATAATTATATTTCAGGCTGCATAACTGACCCACCATATAATTATGAATTTTTTGGTAGAAATTGGGATAATGTAGAAATAAAACGCAGAAAAAA
TAAAGCAAATAACAATAAAAATATATTGGTAAAAAATATACCTTATGGCAGTGGGTTAGCAGGAGGAGTTAAAAATGAAAGATGGTATAAAAAAAATAGG
GATAATATTTTAGAATATCAATCTTGGGTGGAGCATTGGGGGAAAGAGTTATATAGAATTTTAAAGCCTGGTGCGTTTATAATGGTATTTAATAGCAATA
GGAGTGTGGCACATATTCAAATAGCACTTGAAAACGCGGGTTTTTATACCAAAGATATGTTTATATGGATAAGAAATAGTGGTATTCCAAAAGGCTTAAA
TGCTTATGAAAAAATGAAAAAAGATGGATATGAATTGGCAGAAAATTGGAAAGGCTGGCATAGTGCAACAAGAAACAATTACGAGGCAGTATCTGTCTTG
CAAAAACCAATAGAAAACAATTATGTAAACAATGTAAAGAAATACGGAGTAGGACTTTTAAAAACAGAAGGCTATATAAATCATAATGGCTTTCTATCAA
ATATATTTGAAGGCTATCAAAGAGATAAAAAAGATGATTTCAATAAGCATATAACGGTTAAACCAATAAAACTTATAGAAAAATTAGCAGAGATGATTGT
TCCAAAACAAACAAATAATATTATAATTGACCCTTTTATGGGAAGTGGGACAACAGCATTAGCTTGTAAAAATTTAGGTATTCCATATATTGGTTTTGAA
TTAAATGAAGAGTATATTAAAATAGCAAATATGCGATTATCTTAAAAATCATATTTGGCTTTTAAACAAGCCAAATATTGAACTATATCTATTCCACCAT
AGTTTATGATAAAATCAAAAGCATCTTTGTCAGAGATAATTTCCCAACCGCCGTTTATAGCAGCAGTAATAGCCGCTGGTAGGTTATCGTTTCTTAAAAT
CAGGAAAACTGGATTATATCCCATTTCTTTTAACATTTTTCCATATAATTTAAATTTTTTCAGCGTTCCACTATCGCCTGAACCAATTCGATATTTAGCA
TCAATTGCCAAATTACCTATGAAATAATCTACAGGGTGGTCTGTTCCAAAATCAACACTTTCTGGTGGCTTAAATAGATTATTGGAAGTAAAAAGCTCTT
TGGTTATTAATTCTCAAACTCTTCCAAGCTCTCTATTCCAATATTGCATATTTTGCATTTTTGTTTCATAAGTTATATTAAAAAAATCCATTAAAATATC
TGAGTCAGAAACAATTGTGTCAAATTTCTTTGATTTAAAATTACTTTTATATTGCGACAAAATATCAATAACATTACGTTCTATTTGCTCTATAACTATA
TTCATCATTTTAGTAACTCCGACAAATCAGCTCCTAGAGCTTTTGCAATTTTATAAGCATTTATTAGGGTAATATTTTTCTCACATCTTTCTATACTGCC
AATATAAGTTCTATGCAGACTAGATAATTCTGCTAACTTTTCCTGAGTAATATTTAGTGCTTGTCGTCTTTTGCGAACATTTTTTGCAAATATGTTTAAA
ATGGAAATCATAATTTATATTTTATAAATAAGTAACAAAATAAATCAACACTCTATTGATAGCATTTTTTAGAAATCAATACACTATAAAAACAGAGCC

Protein section

ORF number : 4

ORF 4

Length		Begin	End	Strand	Fusion ORF
209 bp	68 aa	2813	2605	-	No

ORF function : Passenger Gene
Annotation : XRE family transcriptional regulatorDescription : Transcriptional Regulator factor

ORF sequence :

MISILNIFAKNVRKRRQALNITQEKLAELSSLHRTYIGSIERCEKNITLINAYKIAKALGADLSELLK

Blast result :

ORF 1

Length		Begin	End	Strand	Fusion ORF
1023 bp	340 aa	85	1107	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MATSNAELEIVKQLFSTLSDDDKKSFLKSIKNKEKSEQNLILSKEIKACPHCKSTNFVKNGKKDGKQRFMCMDCHKTFTVTNNSIFFKTQKDLEIWQKYI
HCMIEKYSLRKTAEICGIALSTAFFWRHKILDTLQKMQDEVKLNGIVEADETFMPISYKGHHKDFNFPRLAKKRGTANTKRGLSKELVCIPCVVNLNGKS
IAKISNLGKPNIASLSKVIQGKVEKESVFVTDSLRSYLKLSNEMSLNHIRIPRNKHSNGSFNIQVVNNYHSRLKSMLVYNFKGVATKYLNNYLVYHNFVN
FAKESRKDKEQILLNHILNTNCISQSISVANRPAVPLLVA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
915 bp	304 aa	1131	2045	+	No

ORF function : Passenger Gene
Annotation : DNA methyltransferaseDescription :

ORF sequence :

MKLSYKKYLNNVTLGDCCELMKNIPNNYISGCITDPPYNYEFFGRNWDNVEIKRRKNKANNNKNILVKNIPYGSGLAGGVKNERWYKKNRDNILEYQSWV
EHWGKELYRILKPGAFIMVFNSNRSVAHIQIALENAGFYTKDMFIWIRNSGIPKGLNAYEKMKKDGYELAENWKGWHSATRNNYEAVSVLQKPIENNYVN
NVKKYGVGLLKTEGYINHNGFLSNIFEGYQRDKKDDFNKHITVKPIKLIEKLAEMIVPKQTNNIIIDPFMGSGTTALACKNLGIPYIGFELNEEYIKIAN
MRLS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
564 bp	0 aa	2605	2042	-	No

ORF function : Passenger Gene
Annotation : Putative restriction endonucleaseDescription :

ORF sequence :

MNIVIEQIERNVIDILSQYKSNFKSKKFDTIVSDSDILMDFFNITYETKMQNMQYWNRELGRVWELITKELFTSNNLFKPPESVDFGTDHPVDYFIGNLA
IDAKYRIGSGDSGTLKKFKLYGKMLKEMEYNPVFLILRNDNLPAAITAAINGGWEIISDKDAFNFIINYSGIDIVQYLACLKAKYDFLR

Blast result :

Comments

ISCaje5 is 63% (transposase) aa similar to ISCco2.

References

1] ISfinder annotation (2017)
2] Harrison,J.W., Dung,T.T., Siddiqui,F., Korbrisate,S., Bukhari,H., Tra,M.P., Hoang,N.V., Carrique-Mas,J., Bryant,J., Campbell,J.I., Studholme,D.J., Wren,B.W., Baker,S., Titball,R.W. and Champion,O.L. (2014) Emerging Infect. Dis. 20 (6), 1026-1029.