ISClsp6
- Family IS1595
- Group ISPna2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ACTE01000011 | ND | Clostridium sp. | Clostridium sp. |
DNA section
IS Length : 1620 bp
Ends
IR Length : 21/24
IRL : GGCTATGTTTTAAATTAGTGTTGAAAATGTATATTTTCAAATATATAGGT
IRR : GGCTATGTTTTAATACAGTGTTGATATCAAGGATAATTTTAATAAGAAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCTATATTT | TAAATAAA | TTATATAATT | 8 |
DNA sequence
GGCTATGTTTTAAATTAGTGTTGAAAATGTATATTTTCAAATATATAGGTTATAATAGAGATATATAAATGTTTCGGAGGTTATAGTATGCCAAATGTAA
AGTCTATATTAACAGATATTCAATCATTACCATTAAATCAAGTAGAGGAATTATTATCTTATTTAGAAGAATTTCTTGTATTAGGTTCTCAAGTAGGACA
AGTATGCGAAGAAGTTAAAGAGTTTAGATTTTCTAAGGGGAAAGTATGTCCTCATTGCAATTCTGAATTAATATCAAGAAATGGTAAATATAATGGTAAA
CAAAGATATATCTGTAAAGATTGTAGAAAGACTTTTACTGATTTTACAAACTCTGCAACATATAGAAGTAAGAAATCTTTAGATAAGTGGCTGAAATATG
CTAAATGTATGATTATGGGGTTATCAATAAGAAAGTCGGCTAAAATTGTAGGTATTAACATAGCAACAAGTTTCTTTTGGAGGCATAAAATACTTGATTG
TATAAGTTCTTTTTTAGGGACTGGTCATGTTGATGGAGTAATAGAAGCAGATGAAGTGTTTTTTGCTGAAAGCTTTAAAGGTACAAGAACTGCTAATATG
CCCAGAAAATCTCGTAAAAGAGGTAAAGAAATAAAAAAAAGAGGTATATCTAAGGAGCAAATTTGCGTGGCTACAGCTTTAGATAGGCAAGGTAATTTAA
TAATAGAGCCTTTGTGTAAAGGTAGGATGACACATAAAGAGCTTGAAAGCCTTTATAAAGGACATATAGGAGAAAATTCTATATTATGTACGGATAGCCA
TAAATCATATATTAAATTTGCTACAGACTTTAATTTAGACCACAAACGCATAAAGACAGGGAAACACAAAGAAGGTATTTACCATATTCAACACATCAAT
AGTTTACACAGTAATTTGAAGAAATGGATGGGTAGATTTAATGGTGTTGCAAGTAAATACATATCAAACTATATGCATTGGTTTAAGTGGCTAAGGTTAT
TTGAAACTGATAGAGATTCTGTAAAAACTAAGAACTTTATTGTACAAAGTAATGTTACATATGCCTACACCAAAATAAAAGATTTCAAATTAAGAACACC
ACAGTTTGTGTAAATAGTAATAAATGATTTAAAAGAATGCTATAATAAAAAGAAAATATGCAATTATTATATATTTTAAAATGAAAAAATTGGAGGAGTA
TAAAAGTATGAGTGATTTTGTTTTGATTATTTTACCATTGATAGCTGTTGCTATTGCAGTGGTTTTATTAGTAAATGATACTAAAAAGAAAAATGAAAAA
AAGGATGATACAGAAGAGAATTATATGTCTATGGGAATGTCATTAGGAATGTGTTTTGGCGTAGCTATAGGTTCAGCATTTACAAATAATTTTGGACCAT
TAAGCATTTCTTATGGTATATGTTTCGGAATGATAGGTGGAATGATTATAGGAATGACGATAAAGAAAAAGTAATTCTACTTAAGTTAAATATAAATGAA
TATACAGAACAATATTAATATAATGCAAACAAGAAAGTAGGGGTATATCAATCAATCGGTATACCCCTACTTTTCTTATTAAAATTATCCTTGATATCAA
CACTGTATTAAAACATAGCC
AGTCTATATTAACAGATATTCAATCATTACCATTAAATCAAGTAGAGGAATTATTATCTTATTTAGAAGAATTTCTTGTATTAGGTTCTCAAGTAGGACA
AGTATGCGAAGAAGTTAAAGAGTTTAGATTTTCTAAGGGGAAAGTATGTCCTCATTGCAATTCTGAATTAATATCAAGAAATGGTAAATATAATGGTAAA
CAAAGATATATCTGTAAAGATTGTAGAAAGACTTTTACTGATTTTACAAACTCTGCAACATATAGAAGTAAGAAATCTTTAGATAAGTGGCTGAAATATG
CTAAATGTATGATTATGGGGTTATCAATAAGAAAGTCGGCTAAAATTGTAGGTATTAACATAGCAACAAGTTTCTTTTGGAGGCATAAAATACTTGATTG
TATAAGTTCTTTTTTAGGGACTGGTCATGTTGATGGAGTAATAGAAGCAGATGAAGTGTTTTTTGCTGAAAGCTTTAAAGGTACAAGAACTGCTAATATG
CCCAGAAAATCTCGTAAAAGAGGTAAAGAAATAAAAAAAAGAGGTATATCTAAGGAGCAAATTTGCGTGGCTACAGCTTTAGATAGGCAAGGTAATTTAA
TAATAGAGCCTTTGTGTAAAGGTAGGATGACACATAAAGAGCTTGAAAGCCTTTATAAAGGACATATAGGAGAAAATTCTATATTATGTACGGATAGCCA
TAAATCATATATTAAATTTGCTACAGACTTTAATTTAGACCACAAACGCATAAAGACAGGGAAACACAAAGAAGGTATTTACCATATTCAACACATCAAT
AGTTTACACAGTAATTTGAAGAAATGGATGGGTAGATTTAATGGTGTTGCAAGTAAATACATATCAAACTATATGCATTGGTTTAAGTGGCTAAGGTTAT
TTGAAACTGATAGAGATTCTGTAAAAACTAAGAACTTTATTGTACAAAGTAATGTTACATATGCCTACACCAAAATAAAAGATTTCAAATTAAGAACACC
ACAGTTTGTGTAAATAGTAATAAATGATTTAAAAGAATGCTATAATAAAAAGAAAATATGCAATTATTATATATTTTAAAATGAAAAAATTGGAGGAGTA
TAAAAGTATGAGTGATTTTGTTTTGATTATTTTACCATTGATAGCTGTTGCTATTGCAGTGGTTTTATTAGTAAATGATACTAAAAAGAAAAATGAAAAA
AAGGATGATACAGAAGAGAATTATATGTCTATGGGAATGTCATTAGGAATGTGTTTTGGCGTAGCTATAGGTTCAGCATTTACAAATAATTTTGGACCAT
TAAGCATTTCTTATGGTATATGTTTCGGAATGATAGGTGGAATGATTATAGGAATGACGATAAAGAAAAAGTAATTCTACTTAAGTTAAATATAAATGAA
TATACAGAACAATATTAATATAATGCAAACAAGAAAGTAGGGGTATATCAATCAATCGGTATACCCCTACTTTTCTTATTAAAATTATCCTTGATATCAA
CACTGTATTAAAACATAGCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 341 aa | 88 | 1113 | + | No |
Chemistry : DDE
ORF sequence :
MPNVKSILTDIQSLPLNQVEELLSYLEEFLVLGSQVGQVCEEVKEFRFSKGKVCPHCNSELISRNGKYNGKQRYICKDCRKTFTDFTNSATYRSKKSLDK
WLKYAKCMIMGLSIRKSAKIVGINIATSFFWRHKILDCISSFLGTGHVDGVIEADEVFFAESFKGTRTANMPRKSRKRGKEIKKRGISKEQICVATALDR
QGNLIIEPLCKGRMTHKELESLYKGHIGENSILCTDSHKSYIKFATDFNLDHKRIKTGKHKEGIYHIQHINSLHSNLKKWMGRFNGVASKYISNYMHWFK
WLRLFETDRDSVKTKNFIVQSNVTYAYTKIKDFKLRTPQFV
WLKYAKCMIMGLSIRKSAKIVGINIATSFFWRHKILDCISSFLGTGHVDGVIEADEVFFAESFKGTRTANMPRKSRKRGKEIKKRGISKEQICVATALDR
QGNLIIEPLCKGRMTHKELESLYKGHIGENSILCTDSHKSYIKFATDFNLDHKRIKTGKHKEGIYHIQHINSLHSNLKKWMGRFNGVASKYISNYMHWFK
WLRLFETDRDSVKTKNFIVQSNVTYAYTKIKDFKLRTPQFV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 1208 | 1474 | + | No |
Annotation : Hypothetical proteinDescription :
ORF sequence :
MSDFVLIILPLIAVAIAVVLLVNDTKKKNEKKDDTEENYMSMGMSLGMCFGVAIGSAFTNNFGPLSISYGICFGMIGGMIIGMTIKKK
Blast result :
Comments
ISClsp6 is 86% aa (transposase) similar to ISCac2.
References
1] ISfinder annotation (2017)
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Daigneault,M.,Strauss,J., Ambrose,C., Allen-Vercoe,E., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Brown,A., Chapman,S.B., Chen,Z.,Dunbar,C., Freedman,E., Gearin,G., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Howarth,C., Larson,L., Lui,A., MacDonald,P.J., Mehta,T., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Yandava,C., Wortman,J., Nusbaum,C. and Birren,B. (2011) Direct GenBank submission.
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Daigneault,M.,Strauss,J., Ambrose,C., Allen-Vercoe,E., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Brown,A., Chapman,S.B., Chen,Z.,Dunbar,C., Freedman,E., Gearin,G., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Howarth,C., Larson,L., Lui,A., MacDonald,P.J., Mehta,T., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Yandava,C., Wortman,J., Nusbaum,C. and Birren,B. (2011) Direct GenBank submission.