ISA1214-1

  • Family ISNCY
  • Group ISA1214
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AE000782 ND Archaeoglobus fulgidus
Archaeoglobus fulgidus
DNA section
IS Length : 1214 bp

Ends


IR Length : 18/21

IRL : GGTTTTGGGACAAAATTCCTTTAATCCCTGACAACAATCACATACGCTCT
IRR : GACTTTGGGACAAAATTACCTCACCACCCTAACAGCAAACACATTGTGCC

Insertion site


Left flankDirect repeatRight flankDR Length
ACGTACTTCATCCTCAAGGGGGAAAAGTATAA12
TGGATTGTATTCCCGAATCTCTGATTCTAA10
TAAAATGCGTGGAGGAAGCGGAAATTTTAA10
AAATAATCACCACATCAAACCCCATATTTTT11
GGTTCAAATCCTGTTAGCAATAAACTTAA9

DNA sequence

GGTTTTGGGACAAAATTCCTTTAATCCCTGACAACAATCACATACGCTCTCCACCCAATATACCTCTTGGGCACATCAACCTTTGCTGAATTACCGAAAG
GCGTGACTCTCTTCTCGAAAACAACCTCAACCTCCTCTTTCAGAACAAAATCTCCCTTCTTCACTTCAACCCTCCTCATGTTAAGTATATCCATAGAAAT
ACTTAAATACTTTTTGGTTGGAATCAGAACATGAACCTTGGATTCAGAGTGACAGGGAAGTTTGTAAGGGAACTTCTGGATGTTTTGGATGAAATTGCAG
AGGAGATAAGACAGGAGGAGAAGGAAAAGTATCCGTACACAGAATGGGAGAGGAAGAGAGAAGTTGTTAAGGAAAGGCTGAGAAAACTCCCTGAATACGT
TAGAGAGGCTATTTCTGTGATAACCGTGCAAAAGAGGGTGGGAAGACCAAAGAAAGTTGATCTGGAGAAGAGAGTTATGCTCTTTCTGTTTGCAAGGCTG
ATGGACAAATCAAACAGAGATATTGAGGAGCTTTTAGAGCTGTTTGAACCTTTATTCGGGATAAAGGTTAGCTACAAAACGATAGAAAGATTATACTCGG
ATGAAGAAGTTAGAATGGCTTTACACAACCTCTTCATCCTTCTGCTTAGAGAGGAAGGAGTTTCAGGAGATTTTTCAGGAGATGGGACAGGATACAGCCT
AACCATCACTAAGCACTATAGAAGCAATCCTAAAAGGAAAGGTAAAGACTTCAGATACGTTTTCAGGATAATTGACATCGATACGGGAATGTACGTTGGC
TTTGGCTATTCTGACAGATCTGAGAAAGATGCCTTTGAGAAGGCTTTAGGAATGCTCAAAAGCATGGGGGTGAAGGTAAACTCGATTTCTCTGGATAAGT
ATTACAGCAGTAGGAAGACTCTGAGGCTGTTTGATGCTGAGACAGCTGTCTACGTCATTCCAAAGCGAAATCTTGCCAGAATAGGATTTGACTGGTTGAG
GGTTATCGAGAGGATTGTTGAAGCTCCTTATAGGTTTCTGAAGAGGTACTTCAAGAGGAACTTAAGTGAGGCAGGATTTTCTGCTGATAAGAGGAGATTT
GGATGGTTGATAAGGCAGAGGAGGGAGGACAGGAGAGAGATGGCTTTGTTTGCTGTTGGGCTGTGGCACAATGTGTTTGCTGTTAGGGTGGTGAGGTAAT
TTTGTCCCAAAGTC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
144 bp47 aa19162-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKKGDFVLKEEVEVVFEKRVTPFGNSAKVDVPKRYIGWRAYVIVVRD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
969 bp322 aa2291197+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MNLGFRVTGKFVRELLDVLDEIAEEIRQEEKEKYPYTEWERKREVVKERLRKLPEYVREAISVITVQKRVGRPKKVDLEKRVMLFLFARLMDKSNRDIEE
LLELFEPLFGIKVSYKTIERLYSDEEVRMALHNLFILLLREEGVSGDFSGDGTGYSLTITKHYRSNPKRKGKDFRYVFRIIDIDTGMYVGFGYSDRSEKD
AFEKALGMLKSMGVKVNSISLDKYYSSRKTLRLFDAETAVYVIPKRNLARIGFDWLRVIERIVEAPYRFLKRYFKRNLSEAGFSADKRRFGWLIRQRRED
RREMALFAVGLWHNVFAVRVVR

 

Blast result :
Comments
See Archaeoglobus fulgidus genome (cf. genome section of ISDatabase).
There are 6 copies of ISA1214 in Archaeoglobus fulgidus at coordinates : 258439-259653 (ISA1214-1); 278689-279901( ISA1214-2); 578102-579314 (ISA1214-3); 763061-764273 (ISA1214-4); 1880751-1881964 (ISA1214-5) and 1987889-1986679 (ISA1214-6).The first 5 copies are isoform but not ISA1214-6.
Note : The authors found a small ORF2 on the complementary strand (length : 47 (19-162).
References
1] Klenk,H.P., Clayton,R.A., Tomb,J., White,O., Nelson,K.E., Ketchum,K.A.,Dodson,R.J., Gwinn,M., Hickey,E.K., Peterson,J.D., Richardson,D.L., Kerlavage,A.R., Graham,D.E., Kyrpides,N.C., Fleischmann,R.D., Quackenbush,J., Lee,N.H., Sutton,G.G., Gill,S., Kirkness,E.F., Dougherty,B.A., McKenney,K., Adams,M.D., Loftus,B.,
Peterson,S., Reich,C.I., McNeil,L.K., Badger,J.H., Glodek,A., Zhou,L., Overbeek,R., Gocayne,J.D., Weidman,J.F., McDonald,L., Utterback,T., Cotton,M.D., Spriggs,T., Artiach,P., Kaine,B.P.,Sykes,S.M., Sadow,P.W., D'Andrea,K.P., Bowman,C., Fujii,C.,Garland,S.A., Mason,T.M.,lsen,G.J., Fraser,C.M., Smith,H.O.,Woese,C.R. and Venter,J.C.(1997) Nature 390 (6658), 364-370