ISAlis1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
FRAF01000028 ND Alicyclobacillus sp.
Alicyclobacillus sp. USBA-503
DNA section
IS Length : 1893 bp

Ends


IR Length : 22/25

IRL : AGCTCTTTTCGTATAGATTGTTGCTCATTGGACAATAATGTATAATGTTC
IRR : GGCTCTTTTCGTAAATATTGTTGCTTTCAACAAGACACTTGTTCATAGAA

Insertion site


Left flankDirect repeatRight flankDR Length
CTTAGTGTTTATAATAAAATTTACATATTAGAAACTAAA8

DNA sequence

AGCTCTTTTCGTATAGATTGTTGCTCATTGGACAATAATGTATAATGTTCGACAAATCTCGTCCGAGGAGCGGTTTCAATGGGTTGGAAGGACCTGTACG
AAGACTTTGATGAATTGAGCCAAGGCGAAAAACTCGCCCTGTTTGAAGCCATTAAGGACACGCTTTTCCCTGAACCTCGGTTAAAGTTGGACAAGGTTGC
GGAAGAAATTCGTGACAGTCGATTCAGTTCGGGATTGGCTTGTGTTCATTGTGGGAGCAAGACGGTTAAACGCAACGGCACCTACCGTAGTCGTCAAAGG
TATCTGTGCAAGGACTGCGGGAAGTCGTTCAATGATACAACTGCAAGTCCTATTGCAGGAACACGATACCCTGATAAATGGCTAAAATTCATCGAGTACA
TGCTGGAAGGTCTGACGTTGCCCAAAATCGCTATGGTGCTCCACATACACGTATCGACGGCGTTCTATTGGCGTCATAAGGTTCTGTACGCCCTTCGCTC
GCTTGGTCATGATGCCCTGAATGGCATCGTTGAATCGGATGAAACCTTCTTCTTGGAATCCCACAAGGGTCGAAAGCCTGTGACCTTCCGAAAGCCACGA
AAACGTGGCGGTGTTGCGAAGAAACGGGGTATCAGCAAGGAACAGGTATGTGTTGTGGTCGCCTTAGATCGAGATGGCGACGTTGTTGCTCAAAATGCTG
GTCGTGGTCGAATCACGGCGACTGAAATCGATGCTGTATTGGGTTCACGCCTTGCTCCAGGTTCCCTGCTTTGTACCGACTCCGCAAAGAATTACATGGC
GTTTGCAAGGATGAAGGGGCTTCATCACGAAGTAATCAATGTACGTAAAGGCGTGTATGTTCGAAAGGGAATCTACCACGTTCAGCACGTAAACGCATAT
CACAAACGATTAAAGAAGTGGATGGAGAGGTTTCAGGGGGTTGCCACCAAGTATCTGGACAACTACCTGTTCTGGTTCCGCTTCTTAGAGCGATACAAAA
ACCTGTCGCCATACGAAGCGAAGCGATCTATCCTTCGAGATATGTCAAGACGGTCCAACGCTGTTACGGTTGAGTTTCTGCGTGCGGTCTAAGTTAGGAG
GAAACGAGTAGAGGTGGATGCAATTGAGGCTGCAAAGCACTTTATTCAATCCCATTTCCCAAGTTGCCGAGCGGCAGTATTGGCTGGGAGCGTTGTAAGA
GGCGAAGCGACAGATACTTCTGACCTCGACATTGTTGTTTTCGACGACAGTATCAGTTCTGCATATCGAGAATCATTATTTCAATACGGGTGGGCAATTG
AAGTGTTTGTTCACAATCTGAAGTCCTATAGGGATTTCTTTGAGAGCGATTGTAAACGAGCACGTCCTTCTCTACCGAGAATGGTACATGAAGGAATTGT
CTTAAAGGACTCAGGAATCATCGATAAAATCAAATCCGAAGCGGGACAACTTCTTAAAAACGGTCCCGCTCCATGGTCATCGGAGACTGTTGCAATGAAG
CGATATATGCTAACCGATACATTGGATGATTTTATTGGTTCTCTCAGGTCGGATGAAGATTTGTTCATCGCCAACACATTGGCGGAAGCCATACAAGAGT
TCTTTTTGAGAACACATCATCAGTGGATCGGGGCTTCCAAATAGATTGTGCGGACATTAAAGGAATACGACGAAGTGTTTGCGAGTGAGTTCATTCATGC
TTTTACGACCTTTTATCAAACAGGAGACAAGGATGAAATAGTGCAACTCACGAACAAAGTGTTAGAACCTTTCGGTGGGCGATTGTTTGACGGATTTTCA
CTTGGAAAACAGAAGGCATAACCCTTTCTATCAGTGCTATGCCTTCTATGAACAAGTGTCTTGTTGAAAGCAACAATATTTACGAAAAGAGCC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1014 bp337 aa791092+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGWKDLYEDFDELSQGEKLALFEAIKDTLFPEPRLKLDKVAEEIRDSRFSSGLACVHCGSKTVKRNGTYRSRQRYLCKDCGKSFNDTTASPIAGTRYPDK
WLKFIEYMLEGLTLPKIAMVLHIHVSTAFYWRHKVLYALRSLGHDALNGIVESDETFFLESHKGRKPVTFRKPRKRGGVAKKRGISKEQVCVVVALDRDG
DVVAQNAGRGRITATEIDAVLGSRLAPGSLLCTDSAKNYMAFARMKGLHHEVINVRKGVYVRKGIYHVQHVNAYHKRLKKWMERFQGVATKYLDNYLFWF
RFLERYKNLSPYEAKRSILRDMSRRSNAVTVEFLRAV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
531 bp176 aa11141644+No
ORF function : Passenger Gene
Annotation : Nucleotidyltransferase domain-containing proteinDescription :

ORF sequence :

MDAIEAAKHFIQSHFPSCRAAVLAGSVVRGEATDTSDLDIVVFDDSISSAYRESLFQYGWAIEVFVHNLKSYRDFFESDCKRARPSLPRMVHEGIVLKDS
GIIDKIKSEAGQLLKNGPAPWSSETVAMKRYMLTDTLDDFIGSLRSDEDLFIANTLAEAIQEFFLRTHHQWIGASK

 

Blast result :
Comments
ISAlis1 is 81% aa similar to ISBci1.
References
1] ISfinder annotation (2016)
2] Varghese,N. and Submissions,S. (2016) Direct GenBank submission.