ISBsm2
- Family IS1595
- Group ISPna2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ACWF01000066 | ND | Bacillus smithii | Bacillus smithii 7_3_47FAA |
DNA section
IS Length : 1497 bp
Ends
IR Length : 21/26
IRL : GGCTATTTTCGTATAGATTGTTGCTATATAGGACATTATGTACAATAGAG
IRR : GGCTCTTTTCTAAAAAATTGTTGCTAATAACCTCAATAAATGTTTACCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTGCAATCT | TTTTTAAA | AGTCCTTTGG | 8 |
DNA sequence
GGCTATTTTCGTATAGATTGTTGCTATATAGGACATTATGTACAATAGAGTCATAAAAGGTAGAAAGGGTGTTCCGTATGGCAATGGCTTGGCACGATGT
TTACCAAGACTTCTATACACTTCCAAAGAAAGAACAGTTGGAATTATTTAGAGCCATTAAAAAGGACTTGTTTCCAGAGCCAAAATCCGACATTTCAAAA
ATGGTCAGAGAGGTTCGTGAAACTCGCTTTTCTAAAGGATTAGCGTGTGTTCACTGTGGAAGTATGTCCGTTAAACGTAATGGGAGATATCGTTCTCGCC
AACGGTATCTATGTAAAGATTGTGGGAAGTCATTCAACGATATGACAGGTAGCCCATTGTCAGGAACAAGATACCCTCATAAATGGCTGAAATACTTTGA
GATGATGGTGAAAGGCTACACATTGCCAAAGATAGCGGAAGAACTTCATATTCACATTTCAACTGCCTTTTATTGGCGACACAAAATTCTATATGCGATT
CGTTCTCTTGGTCATCCAACACTAAAGGGAATCGTGGAAAGAGATGAAACTTACTTTTTAGAAAGTGAAAAAGGCAAAAAGTTCATCGCTCACAGAAAGG
CTCGTAAACGTGGCGGTGTAGCCAAAAAACGTGGCATTTCTAAAGAACAAATTTGTGTGGTAGTTGCACACGATAGAAACGGACAAATCCTTTCCCAAAT
GGTAGGAAGAGGTCGTGTTACGGCTATGGAAATTGATGAGGTGTTAGGAAAACATATTGACACCTCTGCTTTGCTATGCACAGATACAGCAACCAACTAT
AAAAAATTCGCTACGATGAAGGGATTACAACACGAAGCCATTAATGTTCGCAAAGGTGTCTATACGAAGAAAGGAATTTACCATATTCAACACGTCAACG
GGTATCATACCCGTTTGAAAAAGTGGATGGATAGGTTTCAAGGTGTAGCAACGAAATACCTTGATAACTACTTATTCTGGCATCGTTTTCTTGAATTGAA
TAAGAAGATACCGAAAAAAGAACAAGCGAAAGAAATGTTCCTTGCCTCTTGCCAACAAGTAAATTTTACAACTGTTGAAACAATTAGAAGGAGTGCATAA
ATCAATCCCCTTCTAAGGCATTGACCTTGTAATTACTTCAATGACTAACGTAAGAACACCTAAAACGATGGTCATTAAAGTTGAACTGAATAATACTTTT
CTACTGACAACAGTAAATTTTTGGTTTTTAATGAACTTTCGATAAGCAAAATAGACGAGAATACCCAATATAATTGAGAAAATCCAACTTATTAAGCCCA
CTTGTGTAACCAATTTTCGCCAATCGAAAACCCAAAAGTATAGAATGGCGAAATGAATGGCTACTAAGGGTATCGTTATGATTACCAATATTAACCTTAA
CATAATCATCACTCCCAGATTTATTATACCATTATTTTTGAAAAAATTTGGTAAACATTTATTGAGGTTATTAGCAACAATTTTTTAGAAAAGAGCC
TTACCAAGACTTCTATACACTTCCAAAGAAAGAACAGTTGGAATTATTTAGAGCCATTAAAAAGGACTTGTTTCCAGAGCCAAAATCCGACATTTCAAAA
ATGGTCAGAGAGGTTCGTGAAACTCGCTTTTCTAAAGGATTAGCGTGTGTTCACTGTGGAAGTATGTCCGTTAAACGTAATGGGAGATATCGTTCTCGCC
AACGGTATCTATGTAAAGATTGTGGGAAGTCATTCAACGATATGACAGGTAGCCCATTGTCAGGAACAAGATACCCTCATAAATGGCTGAAATACTTTGA
GATGATGGTGAAAGGCTACACATTGCCAAAGATAGCGGAAGAACTTCATATTCACATTTCAACTGCCTTTTATTGGCGACACAAAATTCTATATGCGATT
CGTTCTCTTGGTCATCCAACACTAAAGGGAATCGTGGAAAGAGATGAAACTTACTTTTTAGAAAGTGAAAAAGGCAAAAAGTTCATCGCTCACAGAAAGG
CTCGTAAACGTGGCGGTGTAGCCAAAAAACGTGGCATTTCTAAAGAACAAATTTGTGTGGTAGTTGCACACGATAGAAACGGACAAATCCTTTCCCAAAT
GGTAGGAAGAGGTCGTGTTACGGCTATGGAAATTGATGAGGTGTTAGGAAAACATATTGACACCTCTGCTTTGCTATGCACAGATACAGCAACCAACTAT
AAAAAATTCGCTACGATGAAGGGATTACAACACGAAGCCATTAATGTTCGCAAAGGTGTCTATACGAAGAAAGGAATTTACCATATTCAACACGTCAACG
GGTATCATACCCGTTTGAAAAAGTGGATGGATAGGTTTCAAGGTGTAGCAACGAAATACCTTGATAACTACTTATTCTGGCATCGTTTTCTTGAATTGAA
TAAGAAGATACCGAAAAAAGAACAAGCGAAAGAAATGTTCCTTGCCTCTTGCCAACAAGTAAATTTTACAACTGTTGAAACAATTAGAAGGAGTGCATAA
ATCAATCCCCTTCTAAGGCATTGACCTTGTAATTACTTCAATGACTAACGTAAGAACACCTAAAACGATGGTCATTAAAGTTGAACTGAATAATACTTTT
CTACTGACAACAGTAAATTTTTGGTTTTTAATGAACTTTCGATAAGCAAAATAGACGAGAATACCCAATATAATTGAGAAAATCCAACTTATTAAGCCCA
CTTGTGTAACCAATTTTCGCCAATCGAAAACCCAAAAGTATAGAATGGCGAAATGAATGGCTACTAAGGGTATCGTTATGATTACCAATATTAACCTTAA
CATAATCATCACTCCCAGATTTATTATACCATTATTTTTGAAAAAATTTGGTAAACATTTATTGAGGTTATTAGCAACAATTTTTTAGAAAAGAGCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 78 | 1100 | + | No |
Chemistry : DDE
ORF sequence :
MAMAWHDVYQDFYTLPKKEQLELFRAIKKDLFPEPKSDISKMVREVRETRFSKGLACVHCGSMSVKRNGRYRSRQRYLCKDCGKSFNDMTGSPLSGTRYP
HKWLKYFEMMVKGYTLPKIAEELHIHISTAFYWRHKILYAIRSLGHPTLKGIVERDETYFLESEKGKKFIAHRKARKRGGVAKKRGISKEQICVVVAHDR
NGQILSQMVGRGRVTAMEIDEVLGKHIDTSALLCTDTATNYKKFATMKGLQHEAINVRKGVYTKKGIYHIQHVNGYHTRLKKWMDRFQGVATKYLDNYLF
WHRFLELNKKIPKKEQAKEMFLASCQQVNFTTVETIRRSA
HKWLKYFEMMVKGYTLPKIAEELHIHISTAFYWRHKILYAIRSLGHPTLKGIVERDETYFLESEKGKKFIAHRKARKRGGVAKKRGISKEQICVVVAHDR
NGQILSQMVGRGRVTAMEIDEVLGKHIDTSALLCTDTATNYKKFATMKGLQHEAINVRKGVYTKKGIYHIQHVNGYHTRLKKWMDRFQGVATKYLDNYLF
WHRFLELNKKIPKKEQAKEMFLASCQQVNFTTVETIRRSA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 290 bp | 96 aa | 1403 | 1114 | - | No |
Annotation : Hypothetical proteinDescription :
ORF sequence :
MLRLILVIITIPLVAIHFAILYFWVFDWRKLVTQVGLISWIFSIILGILVYFAYRKFIKNQKFTVVSRKVLFSSTLMTIVLGVLTLVIEVITRSMP
Blast result :
Comments
ISBsm2 is 85% aa similar to ISBci1.
References
1] ISfinder annotation (2016)
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Daigneault,M.,, Strauss,J., Allen-Vercoe,E., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Brown,A., Chapman,S.B., Chen,Z., Dunbar,C., Freedman,E., Gearin,G., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Howarth,C., Larson,L., Lui,A., MacDonald,P.J.P., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B. (2011) Direct GenBank submission.
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Daigneault,M.,, Strauss,J., Allen-Vercoe,E., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Brown,A., Chapman,S.B., Chen,Z., Dunbar,C., Freedman,E., Gearin,G., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Howarth,C., Larson,L., Lui,A., MacDonald,P.J.P., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B. (2011) Direct GenBank submission.