ISA1083-2
- Family IS630
- Group
Isoform Synonym(s) ISA1083-3
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE001010 | ND | Archaeoglobus fulgidus | Archaeoglobus fulgidus |
DNA section
IS Length : 1083 bp
Ends
IR Length : 19/30
IRL : TTTATTGTCCTGAGCAAAACTATTTATACTATAGATAATAGACCAGTTGC
IRR : TTATGGTTAAGTGCATAACATCTTAAACTTATCCCCCAAGAACTTCTCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TTTATTGTCCTGAGCAAAACTATTTATACTATAGATAATAGACCAGTTGCATGGGCAGAAAGCGAGTTTACGAAGTTGTAAAGCACCTACCAGCAGAAGA
ACTCGATAAAAGGATCAAAAAGCTCGAAAAAGATACAAGAGTCCTTCAAAGACTCTACTTCATAAGATACCTTTACAGAGGAATGAGCGTTGAGGAAGCT
GCCGAACTGGTAGGAATTACAAAGGCTACTGGCTACACATGGCTCAAGAGATGGAATACCAGAGGTTATGAAGGCTTAATTCCCGAATTTGGAGGTGGTA
GACCTTCAAAACTTACAAAAGATCAGAAGGAAGAACTCAAAGAAATGCTCAAGGAAAGAGACTCGTGGACAACGAAAGAAGTTCAGGAGCTAATTGAAGC
GAAATTTGGAGTTTCTTACTCTTCGTGGCAGGTCAGGAGAATTCTGAAATCATTCGGCATGAAATACGCCAAGCCCTATCAAAAGGATTACAGAAAGCCT
GAAAATGCTGAAGATTCTTTAAAAAAACATGGATGAAGCCGAAATTCGCCAATCAGACGTAATAGGATTCATCGATGAGATGGCAGTCGAAGCGAATGCA
AATACAGCAAAGCTGTGGAGTTTTGGAAGGCCTGTTAAAAGAGTCGCTACTTACGTCAAAGCTAAGGTTTCAGGATTCTATAGCTTGAACGGGAAAAGCG
TAATAGAGTTTCTTAAGAGTAACAGGTCAGAAGACTTCATATCTTTTCTCAGGAAAATCAGAGAGGAAAATCCCAAGAAAAGGATTGTAATCGTTCTCGA
CAATTTCAAAACGCATCATGCTAAAAAGGTGAAAGAGGAAGCTGAGAAGCTTAGCATTTCGCTGGTTTATCTTCCTCCTTACTCTCCCGATTTGAATCCG
ATTGAAAACGTCTGGAAGAGTGTTAAAAGAGCAGTTTCAGAAACATCTCCTCTGAATGTAGATGAACTTAAAGAAACGATTGCTAAGGCCTTCAAAAAGT
TAACAGGGTCGATATCATTTGCGAAGGGCTGGATTGAGAAGTTCTTGGGGGATAAGTTTAAGATGTTATGCACTTAACCATAA
ACTCGATAAAAGGATCAAAAAGCTCGAAAAAGATACAAGAGTCCTTCAAAGACTCTACTTCATAAGATACCTTTACAGAGGAATGAGCGTTGAGGAAGCT
GCCGAACTGGTAGGAATTACAAAGGCTACTGGCTACACATGGCTCAAGAGATGGAATACCAGAGGTTATGAAGGCTTAATTCCCGAATTTGGAGGTGGTA
GACCTTCAAAACTTACAAAAGATCAGAAGGAAGAACTCAAAGAAATGCTCAAGGAAAGAGACTCGTGGACAACGAAAGAAGTTCAGGAGCTAATTGAAGC
GAAATTTGGAGTTTCTTACTCTTCGTGGCAGGTCAGGAGAATTCTGAAATCATTCGGCATGAAATACGCCAAGCCCTATCAAAAGGATTACAGAAAGCCT
GAAAATGCTGAAGATTCTTTAAAAAAACATGGATGAAGCCGAAATTCGCCAATCAGACGTAATAGGATTCATCGATGAGATGGCAGTCGAAGCGAATGCA
AATACAGCAAAGCTGTGGAGTTTTGGAAGGCCTGTTAAAAGAGTCGCTACTTACGTCAAAGCTAAGGTTTCAGGATTCTATAGCTTGAACGGGAAAAGCG
TAATAGAGTTTCTTAAGAGTAACAGGTCAGAAGACTTCATATCTTTTCTCAGGAAAATCAGAGAGGAAAATCCCAAGAAAAGGATTGTAATCGTTCTCGA
CAATTTCAAAACGCATCATGCTAAAAAGGTGAAAGAGGAAGCTGAGAAGCTTAGCATTTCGCTGGTTTATCTTCCTCCTTACTCTCCCGATTTGAATCCG
ATTGAAAACGTCTGGAAGAGTGTTAAAAGAGCAGTTTCAGAAACATCTCCTCTGAATGTAGATGAACTTAAAGAAACGATTGCTAAGGCCTTCAAAAAGT
TAACAGGGTCGATATCATTTGCGAAGGGCTGGATTGAGAAGTTCTTGGGGGATAAGTTTAAGATGTTATGCACTTAACCATAA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 486 bp | 161 aa | 51 | 536 | + | No |
Description : First part of the transposase
ORF sequence :
MGRKRVYEVVKHLPAEELDKRIKKLEKDTRVLQRLYFIRYLYRGMSVEEAAELVGITKATGYTWLKRWNTRGYEGLIPEFGGGRPSKLTKDQKEELKEML
KERDSWTTKEVQELIEAKFGVSYSSWQVRRILKSFGMKYAKPYQKDYRKPENAEDSLKKHG
KERDSWTTKEVQELIEAKFGVSYSSWQVRRILKSFGMKYAKPYQKDYRKPENAEDSLKKHG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 555 bp | 184 aa | 523 | 1077 | + | No |
Description : Second part of the transposase
ORF sequence :
KNMDEAEIRQSDVIGFIDEMAVEANANTAKLWSFGRPVKRVATYVKAKVSGFYSLNGKSVIEFLKSNRSEDFISFLRKIREENPKKRIVIVLDNFKTHHA
KKVKEEAEKLSISLVYLPPYSPDLNPIENVWKSVKRAVSETSPLNVDELKETIAKAFKKLTGSISFAKGWIEKFLGDKFKMLCT
KKVKEEAEKLSISLVYLPPYSPDLNPIENVWKSVKRAVSETSPLNVDELKETIAKAFKKLTGSISFAKGWIEKFLGDKFKMLCT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1027 bp | 341 aa | 51 | 1077 | + | Yes |
Chemistry : DDE
ORF sequence :
MGRKRVYEVVKHLPAEELDKRIKKLEKDTRVLQRLYFIRYLYRGMSVEEAAELVGITKATGYTWLKRWNTRGYEGLIPEFGGGRPSKLTKDQKEELKEML
KERDSWTTKEVQELIEAKFGVSYSSWQVRRILKSFGMKYAKPYQKDYRKPENAEDSLKNMDEAEIRQSDVIGFIDEMAVEANANTAKLWSFGRPVKRVAT
YVKAKVSGFYSLNGKSVIEFLKSNRSEDFISFLRKIREENPKKRIVIVLDNFKTHHAKKVKEEAEKLSISLVYLPPYSPDLNPIENVWKSVKRAVSETSP
LNVDELKETIAKAFKKLTGSISFAKGWIEKFLGDKFKMLCT
KERDSWTTKEVQELIEAKFGVSYSSWQVRRILKSFGMKYAKPYQKDYRKPENAEDSLKNMDEAEIRQSDVIGFIDEMAVEANANTAKLWSFGRPVKRVAT
YVKAKVSGFYSLNGKSVIEFLKSNRSEDFISFLRKIREENPKKRIVIVLDNFKTHHAKKVKEEAEKLSISLVYLPPYSPDLNPIENVWKSVKRAVSETSP
LNVDELKETIAKAFKKLTGSISFAKGWIEKFLGDKFKMLCT
Blast result :
Comments
See Archaeoglobus fulgidus genome (cf. genome section of ISDatabase).
Note that most IS630 family members have only a single ORF.
ISA1083-2 is 67% aa similar to ISArch3.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
File updated: July 6 2012.
Note that most IS630 family members have only a single ORF.
ISA1083-2 is 67% aa similar to ISArch3.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
File updated: July 6 2012.
References
1] Klenk,H.P., Clayton,R.A., Tomb,J., White,O., Nelson,K.E.,Ketchum,K.A., Dodson,R.J., Gwinn,M., Hickey,E.K., Peterson,J.D.,Richardson,D.L., Kerlavage,A.R., Graham,D.E., Kyrpides,N.C.,Fleischmann,R.D., Quackenbush,J., Lee,N.H., Sutton,G.G., Gill,S., Kirkness,E.F., Dougherty,B.A., McKenney,K., Adams,M.D., Loftus,B., Peterson,S., Reich,C.I., McNeil,L.K., Badger,J.H., Glodek,A.,Zhou,L., Overbeek,R., Gocayne,J.D., Weidman,J.F., McDonald,L., Utterback,T., Cotton,M.D., Spriggs,T., Artiach,P., Kaine,B.P.,Sykes,S.M., Sadow,P.W., D'Andrea,K.P., Bowman,C., Fujii,C.,Garland,S.A., Mason,T.M., Olsen,G.J., Fraser,C.M., Smith,H.O.,Woese,C.R. and Venter,J.C. ,(1997),Nature 390 (6658), 364-370