ISAcde2

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ACQT01000001 ND Acidovorax delafieldii
Acidovorax delafieldii 2AN
DNA section
IS Length : 1032 bp

Ends


IR Length : 23

IRL : GGCTATGTCGCAATTACGTGTTGAAAGGAGTAGCCTGTAGACAGCGACTC
IRR : GGCTATGTCGCAATTACGTGTTGCACAGTTCGCCCGAGGGCTTCTTGCAA

Insertion site


Left flankDirect repeatRight flankDR Length
GCCCGCAGCCTTCGGAAACTGGCTCAAC8

DNA sequence

GGCTATGTCGCAATTACGTGTTGAAAGGAGTAGCCTGTAGACAGCGACTCTTTGCAGGAGGCACGCCATGGACACACAAGCCTTCAGCCACTGGCTGGCC
CAACTCGGTCAGCTCAGCGCCCGGCAGAGAACTGCCTTGCAACAGGCGCTTTTAGCGCCAGCCGTCCCAGATGCTCTGGAGCAAGTCTTGCCGGATCTGA
GACATTGCCCGCACTGCCACGCACGGGCGACGGAACTGGCTGCCTGGGGTTGGAGTCGTGGACTGCGCCGTTACCGCTGCCGCGTCTGCCAGCGCACCTG
CAATACCCTGACGGCCACCCCTTTGGCGCATTTGCGCAAAGCCGACAGATGGCCGGCTTACTGCCAAGCCTTGATCGATGGACTGCCCATCAGGCAGGCG
GCCATGCAATGCGGCATCAGCAAGAACACGGCCTTCCTGTGGCGACATCGCTTTCTCAGGGCCGCATCCGAGCATCAGGCTACGCATCAAGGCGGGATAG
TCGAAGCCGATGAAACCTTCTTTCTTCAATCCCTCAAGGGACAGCGCCATTTGCCGAGATCAGCGCGCAAACGCGGCGGAGTAGGCGCTACCCGAGGAAC
GGGTGAGGACCAGATCCCGGTCATGGTGGTGCGCGATCGGCAGGGTCACACGGCCGACTTTCAGCTAGACAAGCTCGATGCGGCGCATGTGGCTGACGCA
CTGACCCCTTTGATCGATCGCGACGCGGTCTTGTGCACGGATGGCGCAGCTGTCTATGCGGCCTTCGCGCGAACCAGTGGCATCACCCATTGCGTTGTGC
ATGCCCGCAAGGGGCAGCGGATACAAGGCGCCTTGCACATACAGAACGTCAATGCGTATCACGGCAGGCTCAAGCAATGGATGAGCCGCTTTCACGGCGT
TGCCACAAGGTACCTGGCCAGCTATCTGGGCTGGCGGCGAATGCTGGAGCGCTATCAGCGTGCGATTGATACGGCTTGCTGCTTGCAAGAAGCCCTCGGG
CGAACTGTGCAACACGTAATTGCGACATAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
963 bp320 aa681030+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDTQAFSHWLAQLGQLSARQRTALQQALLAPAVPDALEQVLPDLRHCPHCHARATELAAWGWSRGLRRYRCRVCQRTCNTLTATPLAHLRKADRWPAYCQ
ALIDGLPIRQAAMQCGISKNTAFLWRHRFLRAASEHQATHQGGIVEADETFFLQSLKGQRHLPRSARKRGGVGATRGTGEDQIPVMVVRDRQGHTADFQL
DKLDAAHVADALTPLIDRDAVLCTDGAAVYAAFARTSGITHCVVHARKGQRIQGALHIQNVNAYHGRLKQWMSRFHGVATRYLASYLGWRRMLERYQRAI
DTACCLQEALGRTVQHVIAT

 

Blast result :
Comments
ISAcde2 is 73% aa similar to ISAs23.
References
1] ISfinder annotation (2016)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L., Shelobolina,E.S., Picardal,F., Roden,E. and Emerson,D. (2009) Direct GenBank submission.