ISAte2

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JXYQ01000043 ND Acidovorax temperans
Acidovorax temperans KY4
DNA section
IS Length : 1054 bp

Ends


IR Length : 15/17

IRL : GCGAATGCTAGTGATGCCCCTTTAATTGCGCCCCCCTGGCCCTGATCTTC
IRR : GCGAATGCTAGTGACACTTTGATTCCCCATGGCCGCACCCAGCACCAGCG

Insertion site


Left flankDirect repeatRight flankDR Length
ATTCTCAACAGCCCCTATAGAAGAGCCTGATTTTGG0

DNA sequence

GCGAATGCTAGTGATGCCCCTTTAATTGCGCCCCCCTGGCCCTGATCTTCAAGGTACGACGCTCTTGCGTCTGCCCCAGGAGAAGGCCATGCGACAGCAA
CAGATGACGCGCTTGATCCACAGCTTGCCCCAGCTGACGCACCACCAGCTCCAGCAACTGGCAAGCTCTCTGCAGGCTCTGCTCGATCACAGTCAAGCCA
TCGCCGTTTTAGAAGCACACGGCGATGCTCCTCGGGCTTGCCCGCATTGCAGCAGCTCCCTGCTCGTCAAGAACGGCTCGGCCAATGGGCTGCAACGTTT
CAAGTGTCGCCATTGCGCACGCACCTTCAACGCCTTGACTGGCACACCGCTGGCGCGTTTGCATCTGCGGGACAAGTGGATGGGCCAGGCGCAGGCTCTG
GGCGAGGGCCTCTCGCTCAACCAGGTGGCCCAGCGCCTGGGCATAGCGCAAAGCACCGCCTTTCGCTGGCGCCACCGCTTCCTGGCCTGCCCAAGCCACC
AGCAGGCTCACCAGCTTGTGGGCATTGCCGAGGCCGATGAGTCCTACTTCCTGGTCTCCTGCAAAGGCCAAAGAGGGTTGCTGCGCCGTCCTCGCCGGCG
GGGCGGCAAGGCACTGGCTGGCTGCAAGGGTTCGGAGTACACACCCGTACTGATGGCACGAGACCGGGCGGGAGCAACGGTCAACCTCATCCTGCAGACG
AACAAGGCCGTCAGTGTGCAAGCAGCACTCAAGCCCTTGTTGCCACCAGACACCATCCTGTGCACCGATGGCAGCGTCACTCTGGCCAGTGCAGCGCGGC
TCTTGGGTGTGCAGCACCGGGCAGTGAACGTGAGCCGGGGCCAGAGGGTGAAGGGCCCCTGGCATGTGCAGAACGTCAATGCCTATGTCAGCAGGCTGCG
CGGGTGGATGCAGCGTTTCAAGGGCGTTGCAACGAAATACCTTGATTCGTACCTGGGCTGGTTCAGGATGCTGGACCGATCAGGCCCCACGGGGCTGCAG
CCCGCGCTGGTGCTGGGTGCGGCCATGGGGAATCAAAGTGTCACTAGCATTCGC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
966 bp322 aa891054+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRQQQMTRLIHSLPQLTHHQLQQLASSLQALLDHSQAIAVLEAHGDAPRACPHCSSSLLVKNGSANGLQRFKCRHCARTFNALTGTPLARLHLRDKWMGQ
AQALGEGLSLNQVAQRLGIAQSTAFRWRHRFLACPSHQQAHQLVGIAEADESYFLVSCKGQRGLLRRPRRRGGKALAGCKGSEYTPVLMARDRAGATVNL
ILQTNKAVSVQAALKPLLPPDTILCTDGSVTLASAARLLGVQHRAVNVSRGQRVKGPWHVQNVNAYVSRLRGWMQRFKGVATKYLDSYLGWFRMLDRSGP
TGLQPALVLGAAMGNQSVTSIR

 

Blast result :
Comments
ISAte2 is 70% aa similar to ISBeb2.
The STOP codon is done by the insertion site.
References
1] ISfinder annotation (2016)
2] Sheng,K.-Y., Chin,P.-S., Chan,K.-G. and Tan,G.S. (2014) Direct GenBank submission.