ISAma5

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP004850 ND Alteromonas macleodii
Alteromonas macleodii Ionian Sea U4 plasmid
Alteromonas mediterranea U10 plasmid pAMED10_300
Alteromonas mediterranea UM8 plasmid pAMEDUM8_300
Alteromonas macleodii Deep ecotype
Alteromonas macleodii AltDE1 plasmid pAMDE1
DNA section
IS Length : 1019 bp

Ends


IR Length : 19/23

IRL : GGCTATGTTCTGCTAAATTGTTGCTATACTGTATATGAATACAGTTATTG
IRR : GGCTATGTTCGGATTAACTGTTGTTGAATCGAAAAGAGCCTGTTTCCGTT

Insertion site


Left flankDirect repeatRight flankDR Length
CTCACAAGATCTTTTAAAAAAGCAAATTCT8

DNA sequence

GGCTATGTTCTGCTAAATTGTTGCTATACTGTATATGAATACAGTTATTGAGGGTTGATCATGATCCCAGCACAATTTACAAAGCTTATAGCCGAGTTAG
AACACTTAACAGATAGTCAAACCCGTTATGTTGAGAAGCTGCTGAAAGGCACTGATAGCGTCTCACAGTTGATAACAGAGCTTGAGGAGCGGATGGTTGA
AAACCCTGAATGTCCGCACTGTCATAGCTCACTGATCAATCGGCATGGCAAAGTGAATCAGATGCAACGTTATCGTTGCAAGAACTGCGGTAAAACCTTT
GTAGCGACAACGGCGACACCGCTAGCTCGACTAAGATACAAAGAGCGGTGGTTAGAATATATTCGCTGTATGCTGGACAGTAAGCCATTGCGTAAATGTG
CCGAAGAATGTGGTCTCCACCTTGAAACATCGTTCAGGTGGCGGCATCGATTCCTAGCGCTACCATCAGCACTGAAGGCGAGCAAACTAGAAGGTATTAT
CGAGGCTGACGAAACACTTTTTCCCTACTCAGAGAAAGGCTCAAAACGGATGACTCGTCAGCCCCGCAAAAGAGGGATGAAAGCGAAAAAACGAGGGCGT
TCAAAAGATGACTGGGTGCCCGTTTTAACGGTCAGAGACAGAGCCAAGAATACGTATGAGGCCGTTGTGCCAAGTGTTACATCGGCAACTCTGCATCAGG
AGCTAGCAGGAAAATTAGAGAAAGACAGTGTGTTATGTAGTGATGGATATAAGCCCTACATAGCTCTTTCTGAGAAAAATGATTTAATACACAAGCGCTT
AGACGTGGCGGGAGGTGTTAAGGTGATAGATAAAGTCTTTCATATTCAGAATGTTAACGCCTATCACAGTCGTCTGAAAGCGTGGATAAAACGGTTTCAC
GGAGTAGCCACAAAATACCTAGAACATTACCTTGGTTGGTTCAGGTTTATGGATAACCCAGAAAACCTTAACGGAAACAGGCTCTTTTCGATTCAACAAC
AGTTAATCCGAACATAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
957 bp318 aa611017+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIPAQFTKLIAELEHLTDSQTRYVEKLLKGTDSVSQLITELEERMVENPECPHCHSSLINRHGKVNQMQRYRCKNCGKTFVATTATPLARLRYKERWLEY
IRCMLDSKPLRKCAEECGLHLETSFRWRHRFLALPSALKASKLEGIIEADETLFPYSEKGSKRMTRQPRKRGMKAKKRGRSKDDWVPVLTVRDRAKNTYE
AVVPSVTSATLHQELAGKLEKDSVLCSDGYKPYIALSEKNDLIHKRLDVAGGVKVIDKVFHIQNVNAYHSRLKAWIKRFHGVATKYLEHYLGWFRFMDNP
ENLNGNRLFSIQQQLIRT

 

Blast result :
Comments
ISAma5 is 63% aa similar to ISNe7.
References
1] ISfinder annotation (2016)
2] Lopez-Perez,M., Gonzaga,A. and Rodriguez-Valera,F. (2013) Genome Biol Evol 5 (6), 1220-1232.