ISAcif2

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP002985 ND Acidithiobacillus ferrivorans
Acidithiobacillus ferrivorans SS3
DNA section
IS Length : 1402 bp

Ends


IR Length : 23/27

IRL : GGGGTTTAGGGAGCAATAGAACGGAAACCCCCGGTAGTCCATCCGATTTA
IRR : GGGGTTTAGGGCGCAGGGGAACGGAAAGGCTATGTCACCATTGCTTGTTG

Insertion site


Left flankDirect repeatRight flankDR Length
TCTCCGGTTAGATCCCAAGCCTGTC5

DNA sequence

GGGGTTTAGGGAGCAATAGAACGGAAACCCCCGGTAGTCCATCCGATTTAGTGCAATCCACGCTGAACATAGAGGCGTTACCGCGCTTTGGTGGCATGAA
ACCGCCTATGTCCGTGTATAAACCTGTTGCACATCGAGCGTAAAAAGCAATATATTTTCGCAACGTTGGAGCCCAATAAAACCAAGGGGTATTCGTGACG
TGCGCAAACGACCGTTTGTGCAACGGTTACTGCCGTAATTGCCCAGCTTATTCAATGCTCATCACACCACGCTGCCTAAGTGAGTTGCACTATGTTGGCG
GGCAAAATCGCCTTAATGGGGTTTTCCGTTGGCTATGTCATCTTTGATTTGTGGTATACTTCATAACGTGACGAATCAAGGAGATGGCGGCGCGCATGAA
ATCTGATGAATTTGGCAAACTGAAGGGGGCATTGGCGGACCTGACGCCGAATCAGCGCCGGCAGATCAAAGATCAGATCCATGCTGTCGAGCAGCAGCAG
ACCGTACATTTGATCCTGGAATCCCGCGTTGTCGAGCATCCCGCCTGTCCGTATTGCGGAGAAGGGCCGGTTGCTCGGTGGGGTACCGCTTCCGGTTTGC
AGCGGTACCGATGTGGCGCGTGCAAGCATACATTTAACGCATTGACTGGTACCCCATTGGCGGGGTTGCGGCATAAGGAGGTTTGGCTGTCGTATGCGGA
ACAGATGGCGCAAGGGCAAAGCGTGCGCCGGAGTGCCAAGGCCTGCGGTGTGCACCGGAATACGGCCTTTCGCTGGCGGCATCGTTTCCTGAGCCTGCCC
AAGGGACAGCAGCCCACCCAATTGGAAGGTATCGTAGAGGCCGATGAGACCTTCTTCCTGGAGTCCTTCAAAGGCAAGAAAAAGGGGATTCCCCGCGCCT
CGCGCAAACGCGGTGGCAAGGCCAAAAAACCGGGGCTGTCCGATGAACAAATTCCGGTGTTGATTTGCCGGGACCGGGGCGGGCAAACCGCTGACGCCGT
CTTGAAGAAGGTCAACAGCGAAGAGGTTGGAAAGGTTATCCAGCCGATTCTCGCCAAAGACAGCATGCTTTGCGCGGATGGCAGCTCCATTTATGTGCGA
GTGGCACGGGAACTGGATGTACCGCTCAAAGCGGTGAATGTGAAAGCAGGCATCCGCGTCGTGGAACAGGTTTTTCATGTGCAAAATGTGAACGCCTATG
ACAGCCGCCTGAAGACATGGATGCACCGTTTCCATGGGGTGGCCACCGCCTATTTGCCAAACTATCTGGGGTGGCGGCGCTTATTGGATCATTTTCGCGG
AACCGTAGATCCCAAGGTTCTGCTGCGGGCGGCCTTGGGGGTCACCTACCTTCAACAAGCAATGGTGACATAGCCTTTCCGTTCCCCTGCGCCCTAAACC
CC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
978 bp325 aa3961373+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSDEFGKLKGALADLTPNQRRQIKDQIHAVEQQQTVHLILESRVVEHPACPYCGEGPVARWGTASGLQRYRCGACKHTFNALTGTPLAGLRHKEVWLSY
AEQMAQGQSVRRSAKACGVHRNTAFRWRHRFLSLPKGQQPTQLEGIVEADETFFLESFKGKKKGIPRASRKRGGKAKKPGLSDEQIPVLICRDRGGQTAD
AVLKKVNSEEVGKVIQPILAKDSMLCADGSSIYVRVARELDVPLKAVNVKAGIRVVEQVFHVQNVNAYDSRLKTWMHRFHGVATAYLPNYLGWRRLLDHF
RGTVDPKVLLRAALGVTYLQQAMVT

 

Blast result :
Comments
ISAcif2 is 69% aa similar to ISRso22.
References
1] ISfidner annotation (2016)
2] Liljeqvist,M., Valdes,J., Holmes,D.S. and Dopson,M. (2011) J. Bacteriol. 193 (16), 4304-4305.