ISfinder Mirror Site

ISA0963-2

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AE001083	ND	Archaeoglobus fulgidus	Archaeoglobus fulgidus

DNA section

IS Length : 963 bp

Ends

IR Length : 21/25

IRL : TGTAGTATCCCTAAATTATTTCACTACCCACAACCAACTTCATGTGAGAA
IRR : TGTGATATCCCGAAATAATTTCACTCCTCAATCAACCACCTCCCGTACTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAAAATGTCA	TTTTCCTCTAAAGTC	TCGAAAAATG	15

DNA sequence

TGTAGTATCCCTAAATTATTTCACTACCCACAACCAACTTCATGTGAGAAAACTAACGAACAAAAAGATCAGATGGATCATCAAACAACTCGAAAAGGGA
ACCCCAGTAAGAGAAATAGCAGCTGTGATGAGAGTAACACCAAGAAGGATCTACCAACTCAAAAAACAATACGAAGAAACAGGAGAAATTCCAGAACTCA
AACAACCTGGAAGAAAGCCCAAGCCAATAGACAAAGAAACTGAGCAAATAATCCTGCAAGCCTACGAAAAATACAAGCTCAGCCCAGTTCCGCTGGAAAG
GCTGATAGAGAGGGATTACGGCATCCACATCCCCCACAACACTATATACAGAATCTTGCTAAAACACGGTTTGGTGGAGGAGAATATGAATAAGAAAAAG
CGGAGGAAATGGGTTCGTTATGAAAGAAAACACTCAATGAGTTTATGGCAGGGAGACTGGAAAAAGCTTGGAGAGAATTGGATAATAGCCTTCATGGACG
ATGCTTCCCGCTTCATCACCTGCTATGGAGTATTTGAGAAGGCAACGACTGAGAACACGATCAAAGTTCTCAGGAAAGGGTTCGCTGAATATGGAATTCC
AGATGAAATATTGACGGATCACGGAACTCAGTTTGTGGCTGCGAAGAGCAGGGAGAGGGCTAAGCACAGATTTAAGAAATTCCTGGTTGAGAATGGTGTT
AGGCATATTCTTGCCAGAATAAACCATCCTCAAACCAATGGAAAGATTGAGAGGTTCTTTGGACTGATGGAGCAAAAAATTGGTCTTTTTGAATCACTGG
ATGAATTCGTTTACTGGTATAATTACGTTAAACCTCACATGAGCCTTAATTTTGAAGAGCTGGAGACTCCTTATCAGGCTTTTCTCAGGAAGCTGCCTGC
TGAAAGGGTGTTTGAGTACGGGAGGTGGTTGATTGAGGAGTGAAATTATTTCGGGATATCACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
900 bp	299 aa	44	943	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKLTNKKIRWIIKQLEKGTPVREIAAVMRVTPRRIYQLKKQYEETGEIPELKQPGRKPKPIDKETEQIILQAYEKYKLSPVPLERLIERDYGIHIPHNT
IYRILLKHGLVEENMNKKKRRKWVRYERKHSMSLWQGDWKKLGENWIIAFMDDASRFITCYGVFEKATTENTIKVLRKGFAEYGIPDEILTDHGTQFVAA
KSRERAKHRFKKFLVENGVRHILARINHPQTNGKIERFFGLMEQKIGLFESLDEFVYWYNYVKPHMSLNFEELETPYQAFLRKLPAERVFEYGRWLIEE

Blast result :

Comments

ISA0963-2 is 96% aa similar to ISA0963-1 and ISA0963-5, 95% to ISA0963-3, ISA0963-4 and ISA0963-6, 46% to ISVch1, 49% to ISSco1, IS1652 and IS1649 and 38% to IS1122.
Note that there is an apparent extended DR of 15 bp.

References

1] Klenk,H.P., Clayton,R.A., Tomb,J., White,O., Nelson,K.E., Ketchum,K.A., Dodson,R.J., Gwinn,M., Hickey,E.K., Peterson,J.D., Richardson,D.L., Kerlavage,A.R., Graham,D.E., Kyrpides,N.C., Fleischmann,R.D., Quackenbush,J., Lee,N.H., Sutton,G.G., Gill,S., Kirkness,E.F., Dougherty,B.A., McKenney,K., Adams,M.D., Loftus,B., Peterson,S., Reich,C.I., McNeil,L.K., Badger,J.H., Glodek,A., Zhou,L., Overbeek,R., Gocayne,J.D., Weidman,J.F., McDonald,L., Utterback,T., Cotton,M.D., Spriggs,T., Artiach,P., Kaine,B.P.,Sykes,S.M., Sadow,P.W., D'Andrea,K.P., Bowman,C., Fujii,C., Garland,S.A., Mason,T.M., Olsen,G.J., Fraser,C.M., Smith,H.O.,Woese,C.R. and Venter,J.C. (1997) Nature 390 (6658), 364-370