ISAlisp1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
BBSJ01000011 ND Alicycliphilus sp.
Alicycliphilus sp. B1
DNA section
IS Length : 2520 bp

Ends


IR Length : 27

IRL : tgttgaatcacgagcaaaactgagccactgaagggtctccgtcacgtcca
IRR : tgttgaatcacgagcaaaactgagccaggcgtcacgtccaaaactgagcc

Insertion site


Left flankDirect repeatRight flankDR Length
tgcccacctttgtagggacg0
GTTCCGCCGGATGAACAACTTTCAGAGGGCGTT6

DNA sequence

TGTTGAATCACGAGCAAAACTGAGCCACTGAAGGGTCTCCGTCACGTCCAATTTTGAGCCACGTTATCCACCTACCCTGCTGCTTTTTGCAGCAGCGGGG
GACAGGAGTGATCAACGTGAGCACATTGAGCAAATTGCGCCGCATGGTCCACCGTGACCACCTGAGCGTGCGCGAAGCCAGCCGAAGGCTCGGCATCTCT
CGCAACACGGCCGCCAAGTGGTTGGAGGCCAATGAGATGGTCGAGCCCAGCTACCCCAAGCGGGCGACCGTCCCCAGCGTTCTGGATCCGTACAAGGAAC
AGCTGGCCAGCTGGCTGAAGGCCGACAGCCACCGCAACAAGCGCGAGCGCCGTGGCATCAAGGCCATGTTCCGGGCATTGCAGGCCATGGGCTATCCGGG
CAGCCGAGGCCCGGTCTATGAGTTCGCCAAGCGCTGGCAGCAGGCGCAGTCCGATGCGCCCACGCGCATGGCCTTCGTGCCCATGAGCTTCGAGATGGGC
GAGGCCTTCCAGTTTGACTGGAGTTGCGAGTACCTGTTCGTGGGCGGCATGCGCAAGCGCCTGGAGGCCGCGCACACCAAGCTGGCGGCCAGCCGCGCCT
TCATGCTCACCGCCTACTTCAGCCAGGCCCATGAGATGCTGTTTGACGCCCATGCCCGCGCCTTTACTGCCTTTGGCGGCGTGCCACGGCGGGGCATCTA
CGACAACATGAAGACAGCTGTCGACAAGGTTGGCCAGGGCAAGAGCCGCACGGTCAACGCCCGCTTCGAGGCCATGACGGGCCACTACCTGTTCGAGCCT
GAGTTCTGCAACCGCGCCGCTGGCTGGGAGAAGGGCATTGTCGAGAAGAACGTGCAGGACCGCCGGCGCGGCATCTGGATGGAGGCGGCCGAACGCCGCT
GGCCTGACCTGGAAAGCCTCAATGCGTGGCTGCACCAGGCCTGCCTGGACGCCTGGCATGAACTGCCGCACCCCGAGTGGCCCGAGTTGACCATTGCCGA
CGTCTGGCAGCAAGAGCAGACGCACCTGATGCCCAACCCTGCGCCATTCGATGGCTATGTCGAGCAGGTGGCACGGGTAACGGCCACCTCGCTGATCCAC
TACCAGCGCAACCGCTACAGCGTGCCTTGCGAGTGGGCGCACGCAACGGTGAGCCTGCGCGCCTATCCTGACCGCTTGGTGGTGGTCGGCCCCAGCACCG
ATGCGCCCGAGCCGCCAGTGAGCCTGCCACGCAGTTTTGAGCGTGGGCAGACCTTGTACGACTGGCGCCATTACATCAGCCTGCTCGAACGCAAGCCCGG
GGCCTTGCGCAACGGCGCGCCCTTCAAGACCATGCCCGAGCCCCTGCAACATCTTCAGGCGCACCTGCTGCGCCACCCTGGCGGCGACCGCGTGATGGCT
CAGGTGCTCATGGCCATCACCTTGCACGGGCTTGATGACGTGCTGGTGGCCGTGGAGCTGGCGCTGCAATCCGGGCGGGTGAGCGCCGACCATGTGCTCA
ACGTGCTGGCCCGGCTCAAGGAGCCCCAAGCCGTGCAAAGCCTGCCAGATGCAACCTTGCCTTCACTGACGTTGATCGAGCCGCCTCAGGCCGACGTATC
GCGCTATGACCGCCTGCGCCAGCCCCAGGAGAATGACCATGTCCAATGACATCGCAGCAAGCCTCAAGGGTTTGAGCCTGCACGGAATGGCCAGTGCCTG
GCCAGAACTGCTGGGCATCGCACGGCTCAAATCGCTCGACCACGAAGCCCTGCTGCATCAGCTCATCAAGGCCGAGGGCGCACATCGGGAAGTACGCTCC
ATGGCTTACCAGATGCGGGTGGCCCGATTCCCACACCACCGCGACCTGGCAGGCTTTGCATTTGATCAGGCCCAGGTCGACGAGGCACTGGTGCGTCAGT
TGCACGAACTCAGGTTCATCGACTCAGCCCACAACGTGGTCTTCGTGGGCGGCCCTGGCACCGGCAAGACACACCTGGCCACCAGCCTCGGCGTTCATGC
CATCCGTGCACATGGCAAGCGGGTGCGCTTCTTCTCGACCGTAGAACTGGTCAACATGCTGGAGGCGGAGAAGGCGCAGGGCAAGGCTGGCCAAATGGCT
CACAGACTCATGTATGTCGATCTCGTCATCCTCGATGAGATGGGCTACCTGCCCTTCAGCCAAGCCGGCGGTGCCTTGTTATTCCACCTGCTGTCCAAGC
TGTACGAGCGAACCAGCGTGGTCGTCACCACCAACCTGTCGTTCTCGGAATGGGCCAGTGTGTTCGGCGACGCCAAGATGACCACCGCGCTGCTGGACCG
GCTCACCCATTTCTGCCACATCGTCGAGACCGGCAATCAAAGCTGGCGCTTCAGGCACTCGACGGCACAACCGGCCGCCACCAAGTCCAACCGCATCAAA
ACCCCAAAAGGAGCAGACCAGACAGTAAACTTATCCACAACCGAGCAGTCCATTTCTTCGTCAACCTAGTGGCTCAGTTTTGGACGTGACGCCTGGCTCA
GTTTTGCTCGTGATTCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1542 bp513 aa1081649+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MINVSTLSKLRRMVHRDHLSVREASRRLGISRNTAAKWLEANEMVEPSYPKRATVPSVLDPYKEQLASWLKADSHRNKRERRGIKAMFRALQAMGYPGSR
GPVYEFAKRWQQAQSDAPTRMAFVPMSFEMGEAFQFDWSCEYLFVGGMRKRLEAAHTKLAASRAFMLTAYFSQAHEMLFDAHARAFTAFGGVPRRGIYDN
MKTAVDKVGQGKSRTVNARFEAMTGHYLFEPEFCNRAAGWEKGIVEKNVQDRRRGIWMEAAERRWPDLESLNAWLHQACLDAWHELPHPEWPELTIADVW
QQEQTHLMPNPAPFDGYVEQVARVTATSLIHYQRNRYSVPCEWAHATVSLRAYPDRLVVVGPSTDAPEPPVSLPRSFERGQTLYDWRHYISLLERKPGAL
RNGAPFKTMPEPLQHLQAHLLRHPGGDRVMAQVLMAITLHGLDDVLVAVELALQSGRVSADHVLNVLARLKEPQAVQSLPDATLPSLTLIEPPQADVSRY
DRLRQPQENDHVQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
831 bp276 aa16392469+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSNDIAASLKGLSLHGMASAWPELLGIARLKSLDHEALLHQLIKAEGAHREVRSMAYQMRVARFPHHRDLAGFAFDQAQVDEALVRQLHELRFIDSAHNV
VFVGGPGTGKTHLATSLGVHAIRAHGKRVRFFSTVELVNMLEAEKAQGKAGQMAHRLMYVDLVILDEMGYLPFSQAGGALLFHLLSKLYERTSVVVTTNL
SFSEWASVFGDAKMTTALLDRLTHFCHIVETGNQSWRFRHSTAQPAATKSNRIKTPKGADQTVNLSTTEQSISSST

 

Blast result :
Comments
ISAlisp1 is 98% (transposase) and 97% (helper) aa similar to ISAav1.
References