ISAlde2

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP002449 ND Alicycliphilus denitrificans
Alicycliphilus denitrificans BC
Alicycliphilus denitrificans BC plasmid pALIDE01
DNA section
IS Length : 2501 bp

Ends


IR Length : 24/28

IRL : TGTTGATTtccacgcagaagtgacccactaacccccaggatttccatcta
IRR : TGTTGATTTccatgcaaagctgacccaccatttccatcgaatcttgaccc

Insertion site


Left flankDirect repeatRight flankDR Length
aacccatagagcactggccg0
acagccacacaccttgccgt0
gacgcctgggccgtgctttc0
TTTTCATGGACACCGGCTAA0

DNA sequence

TGTTGATTTCCACGCAGAAGTGACCCACTAACCCCCAGGATTTCCATCTAAATCTGACCCACGTACTAACCCTAACCTGCTGCTTTGTTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCCGAGAGATCGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGCAGGCGAGGCCGAGCCGCACTATGCCAAGCGGGTCAGCCCTTCCAAGCTCGATCCCTTTGCCTTGAAGCTC
TCGGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGTAAGCAGCGGCGTACCGTCAAGCAGATATACGCCGATCTGCAGGCGCTGGGCTATACCGGCTCCT
ACAACCGGGTCGCGGCCTTTGTCCGCCTCTGGCAAGAACAACGCCTGGTGGCCCAGCAGACCACCGGCCGCGGCACCTTTGTGCCTTTGGCCTTCGGCCC
CGGCGAGGCGTTCCAATTCGATTGGAGCGAGGACTGGGCAGTGCTCGCAGGTGTGCGCACCAAGCTGCAGGTGGCTCACTTCAAACTCAGTCACAGCCGG
GCGTTCTACCTGCGTGCCTATCTTCTGCAGACGCACGAGATGCTGTTCGATGCCCACAACCATGCGTTCGTCGTGCTGGGCGGTGTGCCCCGCCGAGGCA
TCTACGACAACATGCGCACCGCCATAGACCGCGTGCGCCGGGGCAAGGAGAGAGATGTCAACGCACGCTTTGCTGCCATGGTCAGCCACTTCCTGTTCGA
GGCAGAGTTCTGCAACCCGGCCTCTGGCTGGGAGAAGGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCACCGCCTGTGGCAGGTGGTGCCGCCATTC
CCGAGCCTGCCCGATCTGAATGCCTGGCTTGAGGAGCGCTGTGTGGCCCTGTGGCACGAGATCGAGCATGGCAAGCTGGCTGGCACCGTCGCGGACGTCT
GGGCCGAGGAGCGGGCAGCCTTGATGCCTGTGCCCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCTTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCAGCGCCGTATGCCAACCGGCCTGTGAGCCTGCGGGTCTACGCGGACCGCCTGGTGGTCGCTGCCGAAGGCCAGATCATC
TGTGAACACCAGCGCCTGATCGAGCGCAACCACCATGGCGCTGGGCAGACCGTCTATGACTGGCGCCACTACCTGGCGGTGCTGCAGCGCAAGCCCGGGG
CATTGCGCAATGGCGCTCCCTTCCAGGAACTGCCAGTGGCCTTCAAACGGCTGCAGACCGCCCTCTTGATGCAGCCAGGCGGCGACAGAGAGATGGTGGA
GGTGCTGGCACTGGTGCTGCATCACGACGAGCAGGCCGTGCTCGCCGCAGTGGAACTGGCCCTGGAGTCTGGGGCTGCCAGCAAGACCCATATCCTGAAC
GTGCTGCACCGGCTGCTGGACGGCAAGCCCGCTCCAGCACCGGTCACCTCCCCCCAGGCCCTCAAGCTGGCGATGGAACCCCAGGCCAACGTGCTGCGCT
ATGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCCGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCAG
TTGCGGAACTGGCTGAGCAAGGTGCGCCAGCCTTCGAGGCAGCACAGCCCATCCTGTCCCAACTGCTCAAGGCTGAGACCGCCGAGCGGGAGGTGCGCTC
GGTGGCTTACCAATTGAAGGTGGCCAGGTTCCCCGCGTACCGGGACCTGGCAGGCTTCGACTTCAGCCACAGCGAGGTGAACGAAGCCCTGGTGCGCCAG
TTGCACCGGTGTGAGTTCTTGGAGGATGCCAACAACGTGGTGCTGGTGGGCGGGCCGGGCACAGGCAAGACCCACATCGCCACAGCCCTCGGAGTGCAAG
CCATTGAACACCATCACCGAAGGGTGCGGTTCTTCTCCACGGTAGAGTTGGTCAATGCGCTGGAAGAAGAGAAGGCCCAAGGCAAGACAGGGCAGATGGC
CCACCGGCTTGCATATGTGGATCTGGTGATTCTGGACGAGTTGGGCTACCTGCCGTTCAGTGCATCAGGAGGCGCCTTGCTGTTCCACCTGCTGTCCAAA
CTCTACGAGCGCACCAGCGTCGTGATCACCACCAACCTGAGCTTCAGCGAGTGGGCCAGTGTGTTCGGGGATGCCAAGATGACCACGGCATTGCTCGACC
GGCTCACGCATCACTGCCATATTCTTGAGACCGGCAATGACAGCTACCGGTTCAAGAATAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGTTCACGAATCAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGCTTTGCATGGAAATCAAC
A
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1527 bp508 aa1091635+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLRAGEAEPHYAKRVSPSKLDPFALKLSGWLKTEAGRSRKQRRTVKQIYADLQALGYTGSYNR
VAAFVRLWQEQRLVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYLLQTHEMLFDAHNHAFVVLGGVPRRGIYD
NMRTAIDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFPSLPDLNAWLEERCVALWHEIEHGKLAGTVADVWAE
ERAALMPVPRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIICEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFQELPVAFKRLQTALLMQPGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLAMEPQANVLRYDQ
LREVRYAS*

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
804 bp255 aa16252428+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFEAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDLAGFDFSHSEVNEALVRQLHRCEFLE
DANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKAQGKTGQMAHRLAYVDLVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT

 

Blast result :
Comments
ISAlde2 is 97% (transposase) and 99% (helper) aa similar to ISAlde1.
References