ISAlde1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP002657 ND Alicycliphilus denitrificans
Alicycliphilus denitrificans K601
DNA section
IS Length : 2501 bp

Ends


IR Length : 24/28

IRL : tgttgatttccacgcagaagtgacccactaacccccgggatttccatcca
IRR : tgttgatttccatgcaaagctgacccaccatttccatcgaatcttgaccc

Insertion site


Left flankDirect repeatRight flankDR Length
AAGTCGTCGCGCGTCGTGCACCTTGGGAGCCCACAGGCCA0

DNA sequence

TGTTGATTTCCACGCAGAAGTGACCCACTAACCCCCGGGATTTCCATCCAAACCTGACCCACGTACTAACCCTAACCTGCTGCTTTGCTGAGCAGCAGGA
GACCAGGAGTGATAGACGTGGCAACACTGAGTGTCATCAGGCGCTGGGCCCTGCGAGAGCAGCTGTCCATCCGGGAGATAGCCCGCCGCACGGGCCTCTC
GCGCAACACCATCCGCAAGTACCTGCGCGTAGGCGAGGCCGAGCCGCACTATGCCAAGCGGGTCAGTCCATCCAAGCTCGATCCCTTCGCCTTGAAGCTC
GCTGGCTGGCTCAAGACGGAAGCTGGCCGATCCCGCAAGCAGCGCCGCACCGTCAAGCAGATGTACGTGGATCTGCAGGCGCTCGGTTATGGCGGCTCCT
ACAACCGCGTAGCGGCCTTTGCCCGCCTCTGGCACGAGCAGCGCCTTGTGGCCCAGCAGACCACTGGCCGCGGCACCTTCGTTCCCCTGGCCTTCGGTCC
GGGTGAGGCCTTCCAGTTCGACTGGAGCGAGGACTGGGCTGTTCTCGCCGGCGTGCGCACCAAGCTGCAGGTAGCCCACTTCAAGCTCAGCCACAGCCGA
GCGTTCTACCTGCGCGCCTATCCGCTGCAAACGCACGAGATGCTGTTCGATGCCCACAACCATGCATTCGCAGTCCTGGGCGGTGTGCCCCGCCGTGGCA
TCTACGACAACATGCGCACCGCCGTAGACCGGGTGCGCCGTGGCAAGGAGCGCGACGTCAATGCGCGCTTTGCGGCCATGGTCAGCCACTTCCTGTTCGA
GGCCGAGTTCTGCAATCCTGCTTCGGGCTGGGAGAAGGGCCAGGTGGAGAAGAACGTGCGCGATGCCCGCCATCGCCTGTGGCAGGTGGTGCCGCCATTC
CTAAGCCTGTCCGATCTCAATGCCTGGCTTGAAGAGCGCTGCGTGGCGCTGTGGCACGAGATCGAGCACGGCAAGCTGCCGGGCACCGTCGCGGATGTCT
GGGCTCAAGAGAAGGCAACCTTGATGCCGATGCCCCGGCCCTTCGATGGCTTTGTGGAGCACACCAAGCGCGTCTCACCCACCTGCTTGGTCCACTTCGA
GCGCAATCGCTACAGCGTGCCGGCGCCGTATGCCAATCGACCGGTGAGCCTGCGGGTCTATGCCGATCGCCTGGTGGTCGCTGCCGAAGGCCAGATCGTG
TGCGAGCATCAGCGCCTGATCGAGCGTAACCACCATGGTGCGGGACAGACCGTGTACGACTGGCGCCACTACCTGGCAGTGCTGCAGCGCAAGCCCGGGG
CCTTGCGCAATGGTGCTCCATTCCTGGAACTGCCAGCAGCCTTCAAGCGCCTGCAGGCCGCCCTCTTGAAGCAGCCAGGCGGCGACCGAGAGATGGTGGA
GGTTCTGGCTCTGGTGCTGCACCACGATGAACAAGCTGTGCTCGCTGCAGTGGAGTTGGCACTGGAGTCAGGGGCTGCCAGCAAGACCCACATCCTGAAC
GTGCTGCACCGCCTGCTGGACGGTAAGCCCGCCCCAGCACCGGTCACCTCGCCCCAGGCCCTCAAGCTGTCTGTGGAACCCCAGGCCAACGTGCTGCGCT
ATGACCAATTGAGGGAGGTGCGCTATGCGTCATGACCCTGCCATCGCTTCCATCGTGATCATGCTGCGCGAGCTCAAGATGCACGGCATGGCCCAGGCGG
TTGCGGAACTGGCTGAGCAAGGTGCGCCGGCCTTCGACGCAGCGCAGCCCATCCTGTCCCAACTGCTCAAGGCCGAGACCGCCGAGCGGGAGGTGCGCTC
TGTGGCCTACCAACTCAAGGTGGCCAGGTTCCCTGCGTACCGGGACCTGGCTGGGTTTGACTTCAGCCACAGCGAGGTGAACGAGGCATTGGTACGCCAG
TTGCACCGCTGCGAATTCTTGGAGAACGCCAACAACGTGGTGCTGGTGGGTGGACCGGGTACGGGCAAGACCCACATCGCCACAGCCCTCGGGGTGCAAG
CCATTGAGCACCATCACCGCAGGGTGCGGTTCTTCTCCACGGTGGAGCTGGTCAATGCACTGGAAGAGGAGAAGGCCCAGGGCAAGCCGGGGCAGATCGC
GCACCGCCTAGCCTATGCCGATCTGGTGATCCTGGATGAACTGGGCTACCTGCCATTCAGCACGTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCCAAG
CTGTACGAGCGCACGAGCGTCGTGATCACCACCAACCTGAGCTTTAGCGAGTGGGCCAATGTGTTCGGAGATGCCAAGATGACCACGGCGCTGCTGGACC
GGCTCACGCATCACTGCCATATTCTTGAGACCGGCAATGACAGCTACCGGTTCAAGAATAGCTCCGCACAGCAACCACCACAGACCACCAAGAAGGAGAA
GGCGACCAAGAACTTATCCACAACGTGAGCGTGAAGCTCACGAACCAGGGTGGGTCAAGATTCGATGGAAATGGTGGGTCAGCTTTGCATGGAAATCAAC
A
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1527 bp508 aa1091635+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLRVGEAEPHYAKRVSPSKLDPFALKLAGWLKTEAGRSRKQRRTVKQMYVDLQALGYGGSYNR
VAAFARLWHEQRLVAQQTTGRGTFVPLAFGPGEAFQFDWSEDWAVLAGVRTKLQVAHFKLSHSRAFYLRAYPLQTHEMLFDAHNHAFAVLGGVPRRGIYD
NMRTAVDRVRRGKERDVNARFAAMVSHFLFEAEFCNPASGWEKGQVEKNVRDARHRLWQVVPPFLSLSDLNAWLEERCVALWHEIEHGKLPGTVADVWAQ
EKATLMPMPRPFDGFVEHTKRVSPTCLVHFERNRYSVPAPYANRPVSLRVYADRLVVAAEGQIVCEHQRLIERNHHGAGQTVYDWRHYLAVLQRKPGALR
NGAPFLELPAAFKRLQAALLKQPGGDREMVEVLALVLHHDEQAVLAAVELALESGAASKTHILNVLHRLLDGKPAPAPVTSPQALKLSVEPQANVLRYDQ
LREVRYAS*

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
804 bp267 aa16252428+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPAIASIVIMLRELKMHGMAQAVAELAEQGAPAFDAAQPILSQLLKAETAEREVRSVAYQLKVARFPAYRDLAGFDFSHSEVNEALVRQLHRCEFLE
NANNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEEEKAQGKPGQIAHRLAYADLVILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWANVFGDAKMTTALLDRLTHHCHILETGNDSYRFKNSSAQQPPQTTKKEKATKNLSTT

 

Blast result :
Comments
ISAlde1 is 95% (transposase) and 96% (IS21 helper) aa similar to ISThsp10.
References