ISAisp6

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP000539 ND Acidovorax sp.
Pseudomonas pseudoalcaligenes CECT 5344
Acidovorax sp. JS42
DNA section
IS Length : 2657 bp

Ends


IR Length : 22/28

IRL : tgttggcgccgaccgaaaactgagccacttttgagggtagtgccgaccca
IRR : tgtccgcgccgatccaaatttgagccaccgtgccgactgaatattgagcc

Comments : no DR

Insertion site


Left flankDirect repeatRight flankDR Length
GGAAGGCGGGttcctcgcgc0
ccgcatgtcccccgatatca0
cggacacgatccgagaggtc0
gcggcgtggcctcgacgaac0
TCCGCCAGCTCGCGTTGTGC0

DNA sequence

TGTTGGCGCCGACCGAAAACTGAGCCACTTTTGAGGGTAGTGCCGACCCAAAACTGAGCCAGGTGAACAACCTACTCTGCTGTTTTTTTGTGCAGCAGAG
GACAAGGAGTGATCACCATGGACATGATTGGCAGGATCCGGCATTTGCATGCCCGGAAGAACAAATCGGAGCGCGAGATATCGCGCATGACAGGGCTCTC
GCGCAACACGGTTGCGAAGTGGCTGCACGGGGAAGTGGACGGTCCACCGAAGTACCGGCGCGGCGAGCAGCCCAACAAACTCACGGCGTTCCATGACGTG
CTCAGGCAGGCCTTGAAGGCTGATGCACGTCGGCCCAAACACGAGCGGCGCACAGCCAAGGCGCTGTACGCCGAGATCAGGCGTGCGGGCTATGAGGGTG
GTTACACGCGGGTGACCGACTTCATCCGGGCATGGCGCCAGGGCGAAGGCCAGGGGTCTGCGGGCAACGCCTTCGTGCCGTTGGCCTTCGAGCTGGGCGA
GGCTTTCCAGTTCGACTGGAGCGAGGAAGGCATGGTGATCGGCGGCATCTACTACCGGGTGCAGGTCTCACACATGAAGCTGTGCGCCAGTCGGGCTTTC
TGGCTGGTGGCCTACCCCAGCCAGGGCCACGAGATGCTGTTCGATGCCCACACCCGCAGCTTCGCCGCGCTGGGTGGCGTGGCGCGCCGGGGCATCTATG
ACAACATGAAGACTGCGGTAGACAAGGTCAAGAAGGGCAAGGGGCGCATCGTCAACGAGCGCTTTGCCACCATGTGCGCGCATTACCTGTACGACCCGGA
CTTCTGCAACGTGGCCAGCGGCTGGGAGAAAGGGGTGGTGGAGAAGAACGTGCAAGACAGCCGCCGGCGCATCTGGATCGACGCGGCCAAGATCAAGTTC
GGCAGCTTTACCGAACTCAACGCCTGGCTGGCCCAGCGCTGCCGCGCGTTGTGGGACGAGGTGCGCCACCCCGAGCACACCCAGTTCAGCGTGGCCGAGA
TGCTCGAACACGAGCGCGCGCACCTCATGCCCATGCCCGTGCCGTTCGATGGCTACGTCGAGAAACCCGCTCGGGTCACCAGCACCTGTCTGGTGTCGGT
GGCGCGCAACCGCTATTCGGTGCCGTGCGAACGGGTGGGCCAGATGGTCAGTACGCGGTTGTATCCGGGCAGCGTCGCCATCGTGGCCGACGACACCATC
GTGGCCCGCCACGACCGGCTGAGCAACGCGGGCGAGACCCGGTACGACTGGCAGCACTACATCCCGCTGCTGCAGAGAAAGCCCGGTGCCCTGAGGAACG
GCGCGCCCTTTGCCGACATGCCCGAGCCGCTGCAGCGGCTGCGCCGTGGGCTGCTGCGCAACCCGGGCGGCGACCGGGTGATGGCGCAGGTGTTGGCCAT
TGTGCTCAGCGCCGGTCTGGACGCGGTGCTGGTGGCCATCGAGCTGGCGCTGGAGAGTGGGCCGCCTGGCAAGGTCAGTGTGGAGCACGTGGTCAACGTG
CTGAACCGGCTCAACGCGCAGCCGGTGCCGCCCACGGCGGCCACGCTGCTGAGAGCGACCACGCCGCCGCTGGCGAACACGGCGCGCTACGACAGCCTGC
GCTCGGACACCGTGGGTGCCATCACCGAAGGAGCCGACCATGAAGACTGATGTGCTGGTCGAACTCAAGGCACTGCGCCTGCACGGCATGGCCGGCGCGT
GGGCCGATCTGGTCGAGCAAGGGGGTGGCGAGGCAGGCGGTGTGGGCATCGAGTCCTCGCGCTGGCTGATCGAGCACCTGTTGCAGGCCGAGGGTACGGA
CCGCGCGATGCGCTCGGTCAGCCACCAGATGCACGCGGCGAAGTTCCCGGTGCACCGCGACATGGCGGGCTTTGACTTCGAGGTCTCGCCGGTGGACCGC
AACCTGGTGGAGACTCTGGCCACCACGGCCTTCACCGACGAGGCGCACAACGTGGTGCTGGTGGGCGGACCGGGCACAGGCAAGACCCATCTGGCCACAG
CAATCGGCGTCTCTGGCATCACCCGGCACGGCAAGCGGGTGCGCTTCTATTCCACCGTCGATCTGGTCAACGCACTGGAGCAGGAGAAGGCCCAAGGCAA
GGCCGGTCGCATCGCTACCAGCCTGCTGCGCATGGATCTGGTGATCCTCGATGAACTGGGCTACCTGCCGTTCAGCCAGGCCGGTGGGGCGCTGCTGTTC
CACCTGCTGAGCAAGCTGTACGAGCACACCAGCGTGATGATCACGAGCAACCTGGACTTCGCAGAATGGTCCAGCGTGTTCGGCGACGCGAAGATGACCA
CCGCGTTGCTGGACCGACTCACGCACCACTGCCACATCATGGAGACCGGCAACGAATCGATCCGATTCAGTCGCAGCACGGCCGCAGCCAAGAAGCGCAT
CAAGGCCCGAGAGCAGGCGCGCAAGGGAGCCAAAACCGAAGCTGGAGACGAACCCTTCTGATCGGAAACGGTGATGGGCAAGCCGCTACGGGCTACGCCC
TGCGCGGCTTGCCCATCGCATTTAACAACACGCCAAGGAGACCAGCAAACCAGCACAGCCTCTACACTTATCCACAGCCGACCCTCTCAAAGTCGGCTTC
CACCCCTGGCTCAATATTCAGTCGGCACGGTGGCTCAAATTTGGATCGGCGCGGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1533 bp510 aa1181650+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDMIGRIRHLHARKNKSEREISRMTGLSRNTVAKWLHGEVDGPPKYRRGEQPNKLTAFHDVLRQALKADARRPKHERRTAKALYAEIRRAGYEGGYTRVT
DFIRAWRQGEGQGSAGNAFVPLAFELGEAFQFDWSEEGMVIGGIYYRVQVSHMKLCASRAFWLVAYPSQGHEMLFDAHTRSFAALGGVARRGIYDNMKTA
VDKVKKGKGRIVNERFATMCAHYLYDPDFCNVASGWEKGVVEKNVQDSRRRIWIDAAKIKFGSFTELNAWLAQRCRALWDEVRHPEHTQFSVAEMLEHER
AHLMPMPVPFDGYVEKPARVTSTCLVSVARNRYSVPCERVGQMVSTRLYPGSVAIVADDTIVARHDRLSNAGETRYDWQHYIPLLQRKPGALRNGAPFAD
MPEPLQRLRRGLLRNPGGDRVMAQVLAIVLSAGLDAVLVAIELALESGPPGKVSVEHVVNVLNRLNAQPVPPTAATLLRATTPPLANTARYDSLRSDTVG
AITEGADHED

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
822 bp257 aa16402461+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MKTDVLVELKALRLHGMAGAWADLVEQGGGEAGGVGIESSRWLIEHLLQAEGTDRAMRSVSHQMHAAKFPVHRDMAGFDFEVSPVDRNLVETLATTAFTD
EAHNVVLVGGPGTGKTHLATAIGVSGITRHGKRVRFYSTVDLVNALEQEKAQGKAGRIATSLLRMDLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVM
ITSNLDFAEWSSVFGDAKMTTALLDRLTHHCHIMETGNESIRFSRSTAAAKKRIKAREQARKGAKTEAGDEPF

 

Blast result :
Comments
ISAisp6 is 98% aa (ORF1 : the transposase) and 97% aa (ORF2 : IS21 helper of transposition) similar to ISThsp19.
Additionnal accession number : HG916826.
References