ISfinder Mirror Site

ISVa15

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISEcl9

Accession number	Transposition	Origin	Host
	ND	Vibrio anguillarum	Vibrio anguillarum serovar O2 (VIB12) Vibrio anguillarum 90-11-286 Vibrio mimicus ATCC 33654

DNA section

IS Length : 1097 bp

Ends

IR Length : 19/24

IRL : TACCCGTGATCATTGAAGATGCTTGATTTTCAATAGAATCAGGATCATGC
IRR : TACCGAATCAACTTGAAGATGCAGGCTTGAGAACCTGAAAATGATCATTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTTATGCAAGTTGAACGC	TA	AAAGCTGATTTTTCAGAACGATTGAGTTTG	2
GAGGGCTTTTTATTAACGG	TA	GATAGACTTTGTGGACTCTCGCACAATTAA	2
ATCTACCGTAGTTACATTC	TA	TCAATATTAGAGCAATGATATTGATAGAAT	2
GCTGTCATCTGTGGGATAT	TA	GGCATAATTTCACCATTTTCGGCGTTAATC	2
ATTGAAATTAAATGGTGCA	TA	TAACCTCTTATTACTGCTTCACTCATCTTC	2
AGAAAGAGGAAATTAACTC	TA	GAATCCATGGTTTGGTTACTTGTTGGAATG	2
AGACAAAGTGGTTTAGTTA	TA	GTAGCTATTTTTAGTGGTCTATTACTTGCG	2
TAATAGAACTCATAAGGTC	TA	ACATCTTTTAGTCTGGATTTATTACCAACA	2
TAATAGAACTCATAAGGTC	TA	ACATCTTTTAGTCTGGATTTATTACCAACA	2
TGCCATGAGGCTTTTCCTA	TA	TACAACAACTCTTCATCCGAAAAAATGGCG	2
GGGCATAGGCAAACTGAGA	TA	GATGCATATTATTTCGCTTCAGCGTATGCT	2
TGGTGTGGTTTCTGATATA	TA	AGCTCCTTCATTGATGATGAAGGAGCTGTG	2
GCTTACGATTGTTATCATC	TA	GCAGAATTCCAAAATAGCTTTGAGTATCAC	2

DNA sequence

TACCCGTGATCATTGAAGATGCTTGATTTTCAATAGAATCAGGATCATGCTACAAACCATGAAAATCACACTGACTCCTCAACAGAAACTGCAACTCGAA
CAGATGCACGACATTGAGCGTGATAGTCGAGTTTGCGACCGTATTAAGGCTGTTTTGCTGGCTTCTGAAGGCTGGAGTCAGACTATGATTTCACAAGCTC
TTCGTATTCATGAATCGACCGTTGCACGTCACCTCAGTGACTACGTACTTTCTGAAAAACTTAAGCCTGAGAATGGAGGAAGCCAAAGCAAGCTTTCTGC
TATTCAAACCATGCACCTAATCGAGCATTTGACTGAGAAAACCTATTCTCATACTCATCAAATTGTCGCCTATGTTAAAGAGACGTTTGGGCTTGATTAT
ACTGTTTCTGGTATGAACAAATGGCTTCACCATAATGGTTTTAGCTACAAGCAACCGAAAGGCATACCACACAAGTTTGATGAAGCAAAACAGCAAGCAT
TCATAGAGGCGTATGAAGCGCTAAAGGCAAGCTGTGGCGAGGATGAATCGATAGTCTTTATCGATGCAGTTCACCCAACACTATCAACAAAGATATCGCA
TGGCTGGATACGTACTGGTCAGGATAAAGTGATTGAAACAACGGGTAATCGTAGCCGATTGAACATTATTGGCGCACTGAACCTGTCGGATATTGGTGCA
ACCATTGTTCACGACTATGAGAGCATTAACAGTGAATCGATTGTTCGTTTTTTCTGTAAGTTAAGAGAGAGTTATTCGTTAGCCCATAAGCTTCATATCA
TATTAGATGGTGCGGGATATCACCGCAGTGACTTAGTCAAAGATGCGGCGTTTGTCCTGAATATTAAACTGCATTATCTTCCACCTTACAGTCCAAACCT
CAACCCAATAGAGCGGCTATGGAAAGTAATGAATGAGAAGTCGAGGAACAACGTTTACTTCAAAAGAAAACGGGACTTCAAGGCGGCAATAGACCAATTT
TTTTCTGTGACTCTTCCAGAGATCGCAGGCTCTTTGACATCTCGAATTAATGATCATTTTCAGGTTCTCAAGCCTGCATCTTCAAGTTGATTCGGTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1044 bp	347 aa	47	1090	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLQTMKITLTPQQKLQLEQMHDIERDSRVCDRIKAVLLASEGWSQTMISQALRIHESTVARHLSDYVLSEKLKPENGGSQSKLSAIQTMHLIEHLTEKTY
SHTHQIVAYVKETFGLDYTVSGMNKWLHHNGFSYKQPKGIPHKFDEAKQQAFIEAYEALKASCGEDESIVFIDAVHPTLSTKISHGWIRTGQDKVIETTG
NRSRLNIIGALNLSDIGATIVHDYESINSESIVRFFCKLRESYSLAHKLHIILDGAGYHRSDLVKDAAFVLNIKLHYLPPYSPNLNPIERLWKVMNEKSR
NNVYFKRKRDFKAAIDQFFSVTLPEIAGSLTSRINDHFQVLKPASSS

Blast result :

Comments

ISVa15 is 84% aa similar to ISVch6.
Multispecies transposase (mainly in Vibrios).

References

1] Holm K.O. (2016) Direct submission
2) Busschaert P. et al (2014) Journal of Fish Diseases (doi:10.1111/jfd.12290)
3] VIB12 [in Austin B. et al (1995) Systematic and Applied Microbiology 18: 285-302