ISEc69
- Family IS1595
- Group IS1016
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LT598652 | ND | Escherichia coli | Escherichia coli Escherichia coli plasmid pKP37-BE |
DNA section
IS Length : 714 bp
Ends
IR Length : 16/19
IRL : GGGGCTGTACTAGATTAGCAACAATGCTATACTAGTTTTATGAAGATAAC
IRR : GGGGCTGTACCAGATAAACCTTTAAATCCCACACCATTTTCTTAATAATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATATTGCGTATT TAAATAAA | TTTATTTATCTGCAC | 0 | |
| CGCCTGTATTTATCGTTA | TAAATAAA TTCCTCAACA | 0 |
DNA sequence
GGGGCTGTACTAGATTAGCAACAATGCTATACTAGTTTTATGAAGATAACCCGTTGTAAATTAAGTAAAAAAGTTCAAGTTAAACTGCTTGAATTTTTTG
TGCTTGAAGTCACCGCAAGATCAGCTGCTGATTTGTTAGAGATACATCACAATTCAGCCGCTTTGTTTTATCATAAAATTCGCTTGGTGATTGAATATCA
CTTAAACCTTGAAGCTTGTGAATTATTTGATGGTGAAGTAGAATTGGATGAAAGCTATTTCGGTGGCATTCGTAAAGGCAAGCGTGGGCGTGGAGCTGCT
GGCAAAGTTGCCGTATTTGGTCTTTTAAAACGCAATGGTAAGGTTTATACCTCCTGTGTTAAAGATACCAAAGCCAAGACATTGATGCCTATTATTGCCA
GTAAAATCAAACCAGATAGCGTGGTTTATACAGATAGCTATCGAAGTTATAATGCTTTAGATGTGAGTGATTTTAAACACTTTAGAATTAATCATTCCAA
AGAATTTGCAAACAATCATAATCACATTAACGGCATTGAAAACTTTTGGTCGCAATCCAAACGCATTCTAAGAAAATATAACGGTATTGACAAAAAGCAT
TTTCATTTATTTATCAAAGAATGCGAGTTTAGATTTAACTATGGCGCACCATCCAACCAATTAAGATTATTAAGAAAATGGTGTGGGATTTAAAGGTTTA
TCTGGTACAGCCCC
TGCTTGAAGTCACCGCAAGATCAGCTGCTGATTTGTTAGAGATACATCACAATTCAGCCGCTTTGTTTTATCATAAAATTCGCTTGGTGATTGAATATCA
CTTAAACCTTGAAGCTTGTGAATTATTTGATGGTGAAGTAGAATTGGATGAAAGCTATTTCGGTGGCATTCGTAAAGGCAAGCGTGGGCGTGGAGCTGCT
GGCAAAGTTGCCGTATTTGGTCTTTTAAAACGCAATGGTAAGGTTTATACCTCCTGTGTTAAAGATACCAAAGCCAAGACATTGATGCCTATTATTGCCA
GTAAAATCAAACCAGATAGCGTGGTTTATACAGATAGCTATCGAAGTTATAATGCTTTAGATGTGAGTGATTTTAAACACTTTAGAATTAATCATTCCAA
AGAATTTGCAAACAATCATAATCACATTAACGGCATTGAAAACTTTTGGTCGCAATCCAAACGCATTCTAAGAAAATATAACGGTATTGACAAAAAGCAT
TTTCATTTATTTATCAAAGAATGCGAGTTTAGATTTAACTATGGCGCACCATCCAACCAATTAAGATTATTAAGAAAATGGTGTGGGATTTAAAGGTTTA
TCTGGTACAGCCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 654 bp | 217 aa | 40 | 693 | + | No |
Chemistry : DDE
ORF sequence :
MKITRCKLSKKVQVKLLEFFVLEVTARSAADLLEIHHNSAALFYHKIRLVIEYHLNLEACELFDGEVELDESYFGGIRKGKRGRGAAGKVAVFGLLKRNG
KVYTSCVKDTKAKTLMPIIASKIKPDSVVYTDSYRSYNALDVSDFKHFRINHSKEFANNHNHINGIENFWSQSKRILRKYNGIDKKHFHLFIKECEFRFN
YGAPSNQLRLLRKWCGI
KVYTSCVKDTKAKTLMPIIASKIKPDSVVYTDSYRSYNALDVSDFKHFRINHSKEFANNHNHINGIENFWSQSKRILRKYNGIDKKHFHLFIKECEFRFN
YGAPSNQLRLLRKWCGI
Blast result :
Comments
ISEc69 is 85% aa similar to IS1016C2.
Two copies of this IS constitute a composite transposon in the plasmid pKP37-BE (LT598652). So the IS itself in this plasmid does not have DR, but the whole composite transposon has 8bp DR (TAAATAAA). This composite transposon carries mcr-2 resistance gene.
Two copies of this IS constitute a composite transposon in the plasmid pKP37-BE (LT598652). So the IS itself in this plasmid does not have DR, but the whole composite transposon has 8bp DR (TAAATAAA). This composite transposon carries mcr-2 resistance gene.
References
1] Susu He (2016) Direct submission
2] Sally Partridge (2016) Direct submission
3] Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, Malhotra-Kumar S.(2016) Euro Surveill. 21 (27).
4] Partridge SR (2017) J Antimicrobial Chemother 72(5):1533-5.
2] Sally Partridge (2016) Direct submission
3] Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, Malhotra-Kumar S.(2016) Euro Surveill. 21 (27).
4] Partridge SR (2017) J Antimicrobial Chemother 72(5):1533-5.