ISCfr8

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Citrobacter freundii
Citrobacter freundii
DNA section
IS Length : 2397 bp

Ends


IR Length : 22

IRL : TGGCCATTAATTTTGACCCACCCCAAGGTGGCCAACCGGCCAGAAATGCA
IRR : TGGCCATTAATTTTGACCCACCTTTGGCCAATAAAATTGACCCACCCTTG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGGCCATTAATTTTGACCCACCCCAAGGTGGCCAACCGGCCAGAAATGCAGATAGGGTTCCCAGCCTTTTGGCAGGCCGGGAACCGTTCTGATGATGAGG
TGGAACGTGATACACAAAATAAAATCATTACATGACGATGGCAAAGGACTTTCCATTCGGGCCATCAGCCGGGAGCTGGGCATCTCGCGCAACAGCGTGC
GCAAGTATCTGCGTCAGGATGAAACAACCACTGCCCGCAAGCTGGATAACCCTGTCCGCAGCAAACAGCTCGACACCTACCGGGGATACCTGATCAGCTT
GCTGGCCAGCTTTCCCGGGCTCAGTGCAGTCAAGATTGCCCGCAAGCTCAAGGAGAAACTGGGTGAGCTGCCGACCTCCGAGCGCAGTATTCGTCGTTAC
GTCAGCGCGCTGAAGGAAGAGGTTGCAACCGGTCAGCACCGATATTACGAGCCGGTGGTTGATGAGGTGCCGGGTGTGCAGTGCCAGGTTGACCCGGGCG
AGCTGCGTAACGTGCTGATTGGTGGCGTGGAAACAGTGGTGCACTTTGTTGTGTTTGTGCTGGCGTACTCGCGGCTGATGTATGTGGGCCTGTCGTTTAA
GCCGCTGGATACCCGGCAGTTCATCCAGCTGCATGACGAAGCGCTGCGCTACTTTGGCGGCATGCCTGAAGAGCTGGTGTACGACCAGACCAAGCTGGTG
GTGATTGAGGAGAAATACCGGGAGCTGACCCTCAACGAGCGTTTTCATCAGTACGCCACCACAGCCGGCTTTCGTATCCATGCTTGCGAAGGCTATGACC
CCGAGAGCAAGGGCAAGGTTGAAGCGGGCGTCAAATACGTCAAGCAGGACTGCTTTTACGGCGAGACCTTCCGTGATGAGCAGCACCTGCGCGAGCATGT
GCATCACTGGCTGGACAAGGTCGCCAATGTGCGGGCACACGGCACCACAGGTGAGGCACCTCAGCTGCGTTTTGAACGTGACGAACAGGCTCTGCTCAAG
CCCTACCTGTCACCCTCTTGCGTCACATCTGAAGCCCGTGAGACCCGCAAGGCGGATAAGACCGGACTGATCTCCTGGAAGGCCAACAAGTACTCTGTGC
CCATGGCCTGGCAGCAAAGCCGTGTCGGTGTGCTGGAAGACGACAGCCAGCTGCATGTGATTGATCTACAAACTGGCGAAGTGATTGCCCAGCATGCGTT
GGCGGGCGGCAAAGGTCAGATTATCAAAAACACCCACCACTACCGTGACCATGCCCAGCGCCGGCAGCAGCTGGAGCAGGACATTCAAGTACTGGTGGGC
GACGAGGCACTCAGCCTGGCCTTGTGTGATCAGTTGCGGAGATCCTTGCCCAAGGTCTATAAGGATCAACTGTTCGCAGTACGCAACCTGCTGAGTAAGC
ACGCACCTGTTGAACGGGACATGCTGTTGAACCTGAGCCAGAAGCCGGGCCTGACCGCCCGACAGATCGAAGCGTATCTGGTGGCCACCCAGCTGGCCAG
CCGGCGTGAGCGTCTGCCTGAGCCCGTGCCAGAGGCTCCGTCAGAGCTGGACTTGAGTGCATACCGACAGCTTGGCCGCTCGACTGGCCAGGAGGTGACC
CATGGGCATGCTTGAACAGACTGCCGCCCAGTACCGTAGCCTGCGCATGACCTCCATCGCCGCAGAGCTCGCCCAGTTACTGGCCGAGGCCGAAGCCAGT
GAAATGTCGTTCCTGAGCTTTGCACAGCGACTGGCTGAATACGAACTCCAGCAGCGTCAGGCCAACCGGGTACAGCGCAATTACAAGACGGCAGGCTTTC
CTGCGCACAAGCATCTGGAGGGCTTTGACTATCGCCACCAGACCACCATCAGCAAGCGCCAGATCAACAGCCTGCTGGACTTCCGGTTCATCGACGAGCG
CAACAACGTGATCTTCATCGGCCCGCCAGGCGTGGGCAAGACCCACCTGGCCATCGGGATTGGCCACAAGGCCGTCGAGGCCGGATACCGGGTATTGTTT
CGCACCGCGCTGGAGTTGGTGGAAGAACTGGAGCTGGCAGAAATGAAGGGCGAGCTGAAAAAGAAAATCAGCCAGCTGAGCAAGGTCGACCTCTTGATCA
TCGATGAGCTGGGCTACCTGCCCATGACCCGGCAGGCCCGCTACAACCTGTTCCAGCTGATCAACAGTCTGTACGAATACCGGTCTATCATCCTGACCAC
CAACAAGGACTTCACCTGCTGGGGCGAGTTCTTCCACGACGACAACGTGGCAGTACCGATCATCGACCGAATCATCCATCACTCACACATCTTTATGCTG
GGAGGAGAGAGCTATCGGCTGAAACAGAAAACAACGGGTTAAACGTGCAAGGGTGGGTCAATTTTATTGGCCAAAGGTGGGTCAAAATTAATGGCCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1509 bp502 aa1071615+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VIHKIKSLHDDGKGLSIRAISRELGISRNSVRKYLRQDETTTARKLDNPVRSKQLDTYRGYLISLLASFPGLSAVKIARKLKEKLGELPTSERSIRRYVS
ALKEEVATGQHRYYEPVVDEVPGVQCQVDPGELRNVLIGGVETVVHFVVFVLAYSRLMYVGLSFKPLDTRQFIQLHDEALRYFGGMPEELVYDQTKLVVI
EEKYRELTLNERFHQYATTAGFRIHACEGYDPESKGKVEAGVKYVKQDCFYGETFRDEQHLREHVHHWLDKVANVRAHGTTGEAPQLRFERDEQALLKPY
LSPSCVTSEARETRKADKTGLISWKANKYSVPMAWQQSRVGVLEDDSQLHVIDLQTGEVIAQHALAGGKGQIIKNTHHYRDHAQRRQQLEQDIQVLVGDE
ALSLALCDQLRRSLPKVYKDQLFAVRNLLSKHAPVERDMLLNLSQKPGLTARQIEAYLVATQLASRRERLPEPVPEAPSELDLSAYRQLGRSTGQEVTHG
HA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
741 bp246 aa16022342+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MGMLEQTAAQYRSLRMTSIAAELAQLLAEAEASEMSFLSFAQRLAEYELQQRQANRVQRNYKTAGFPAHKHLEGFDYRHQTTISKRQINSLLDFRFIDER
NNVIFIGPPGVGKTHLAIGIGHKAVEAGYRVLFRTALELVEELELAEMKGELKKKISQLSKVDLLIIDELGYLPMTRQARYNLFQLINSLYEYRSIILTT
NKDFTCWGEFFHDDNVAVPIIDRIIHHSHIFMLGGESYRLKQKTTG

 

Blast result :
Comments
ISCfr8 (transposase) is 74% aa similar to ISCARN89.
References
[1] Zhou D. (2016) Direct submission