ISPlu20

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
BX470251 ND Photorhabdus luminescens
Photorhabdus luminescens subsp. laumondii TTO1
DNA section
IS Length : 2339 bp

Ends


IR Length : 20/25

IRL : GTAAACGTCCGGTCATCTCACCTTGTTCTTCCTCTCCGGCCCATGCACGC
IRR : GTAATGTTCCGGTGAACTCACCTTGCGTGGTCAGATAACCACAAAATGGG

Insertion site


Left flankDirect repeatRight flankDR Length
ATAGCGTGCATAGTTCATCTAGGCATGTTTTC8
AGGGATATTCTGTTTCGTTCTCATTTGATGCG8
ACCAACCGTGACAGTTGATAGCCCGAAAGGAT8
TGAAAACAGGTCAGTACTGCCCGATCGGCGGC8
GATGTTTTTATTGATTAAAGCATCAAAGTGAG8

DNA sequence

GTAAACGTCCGGTCATCTCACCTTGTTCTTCCTCTCCGGCCCATGCACGCTACCGCTTCCTTATCTTCACCTGGAGACTGACACCATGAGCCGTAGCCGA
TATACCCCGGAACAAAAACAACACCATGTGACCCAATGGCGCCACAGTGACCTGACCCGAAAACAGTATTGCGAACAGCATCAGCTGAGTTTTTCCGCTT
TCCGCGACTGGATTGCCGACAGTAACAACATCCCCCAACCTCTCAGCCAGACACTGCCCGCCCTCTTACCGGTTTCACTCCAGCCTGACGACGCGCGCAC
CGTCACCCTGCATACCCCAGACGGCTATGCCATCGCCTGTCCATTGACGCTGTTGCCTGACGTGATGCGGGTACTGACCCGATGCTGAAACCGCAACAGC
TCTTTCTGGTGCGAGAGCCCGTCGACATGCGCCGGGGCATTGATGCCCTGACCCAGCACCTGGAGGGGCTGAATTTGCGCTGGCAGGACGAAGCCGCTTT
TATTTTCTGCAACAAGGCCCGCTCCCGTCTCAAGGTGTTGCGCTGGGACCGACACGGCGTCTGGCTGTGTACCCGCCGTCTGCACCGGGCCCATTTTGTC
TGGCCGAAGCAGGGCGAGCGCAGCTGGGTGATGACGCCCGCCCAGTTTGACTGGCTCATCCGCGGCATTCACTGGCAACAGGTCGAGGGAGATGACCTGT
CCGGCTGGCGAAACTAATTCCGCTACCCCGGTCACACTTTTACCGGATAACGACGGGGAATTATCCGGTACACTGTCGGCATGACGCTCAATGACTTAAT
CGCGCTGGATGACCCCGGCCAGTTCCGCCTGCGGGCACTGGCGCTGCTTCAGCAACAAGGTGATTATATTCGCCAGCTGGAAGAGGCCCTGAAACAGGCG
CAACGCTGGCGTTTTGGCGCGCACAGCGAAACCCTGCCTGCCGGTCCCAAACGCAGTCAGTTTGAGGAAGATGCCGATACCGATATCGCGGTGCTGGAAA
CCCGGCTGTCACGCCTGCATATCAAGGAAAACGCGACCCCGGCCCAGCCTAAACGTCAGCCTTTGCCGGCGACTTTACCCCGTGAAGATATCCGGCTGGC
GCCGGAGACGGAGAGCTGCCCGGATTGCGGTCATGCCCTGCGCTTTTTGCGTGATGAAATCAGTGAGCGACTGGAATACCGTCCGGCCACGTTTATCGTC
CGGCGCTATATCAGCCCGCAGTACAGCTGCGCGCGCTGTCAGTGTGTGCATGCTAAAGCCCAGCCCGCCCATCTTATTGAAAAGGGCCTTCCAGAGCCGG
GGTTGCTGGCTCAGGTGGTGGTCGCTAAATATCGTGACCACCTGCCGCTCTACCGTCAGCAGCAAATCTATGCCCGCAGCGGTGTCACCCTGGCCCGCAG
CACCCTGTCGGACTGGGTGGGGCAGGTGGCGGTGGCGTTGCAACCGTTAGCCGATGCCCTGAAGCAGACGCTGCTGACGTCCCCGGTGCTGCATGCCGAT
GAGACCCCGTTGCCGATACTGGCCCCGGGAAAAGGCCAGACCCAGCGAGCTTACCTGTGGACGTCTGTCACCGGCCCCGATACCTCGCCGGCAGTGGTAT
ACTATGAGCTGCATCCCGGCCGCAGCGGCCGTTATGCCCAGAGCCTGCTGAAAGACTGGTCGGGCGGCACGTTGGTGACGGATGACTATGCCGGTTACAA
CGCACTGCATGCCCGGGCGGATATCACCGAAGCGGGTTGCTGGGCTCACGCCCGCCGCAAATTCTTTGACCAATACAAAGCGAGCCAAAGTCCGGTGGCG
AAACAGGCGCTGGATGGCATTCGTGACCTGTACAAGCTGGAGCGAAAAATCAAACACCGGCCGCCGGATAAACGGCGTCAGTGGCGGCAGCGTTATGCCC
GGCCGTGGCTGAATGAGTTCCGGTCCTGGTTGCAAACGATGCAGACCCAAACGGCCCCCAACTCGGGGTTACGCAAAGCGATAGATTACACGCTGAAGCG
CTGGTCGGCGCTGGTGTGTTATCTGGATGACGGGGGGGTGCCGATAGACAACAACCGGGCGGAGAATGCGGTCCGGGGTGTGGCGCTCGGCCGAAAGAAC
TGGCTTTTTGCGGGTTCACTGGCCGCGGGGCAACGGGCGGCCATGATAATGAGCCTGCTGGAAACGGCCAAAGCCAACGGACATGAGCCCTGGGTCTGGT
TACGTGATGTCCTCAGCCGGCTGCCTGTCTGGCCGAACAATCGGTTGAATGAGCTGTTGCCCTGGCCTGAGAATCCCTTCCGTTAACATCCCATTTTGTG
GTTATCTGACCACGCAAGGTGAGTTCACCGGAACATTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
303 bp100 aa86388+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSRSRYTPEQKQHHVTQWRHSDLTRKQYCEQHQLSFSAFRDWIADSNNIPQPLSQTLPALLPVSLQPDDARTVTLHTPDGYAIACPLTLLPDVMRVLTRC

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
336 bp111 aa382717+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MLKPQQLFLVREPVDMRRGIDALTQHLEGLNLRWQDEAAFIFCNKARSRLKVLRWDRHGVWLCTRRLHRAHFVWPKQGERSWVMTPAQFDWLIRGIHWQQ
VEGDDLSGWRN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1503 bp501 aa7812283+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTLNDLIALDDPGQFRLRALALLQQQGDYIRQLEEALKQAQRWRFGAHSETLPAGPKRSQFEEDADTDIAVLETRLSRLHIKENATPAQPKRQPLPATLP
REDIRLAPETESCPDCGHALRFLRDEISERLEYRPATFIVRRYISPQYSCARCQCVHAKAQPAHLIEKGLPEPGLLAQVVVAKYRDHLPLYRQQQIYARS
GVTLARSTLSDWVGQVAVALQPLADALKQTLLTSPVLHADETPLPILAPGKGQTQRAYLWTSVTGPDTSPAVVYYELHPGRSGRYAQSLLKDWSGGTLVT
DDYAGYNALHARADITEAGCWAHARRKFFDQYKASQSPVAKQALDGIRDLYKLERKIKHRPPDKRRQWRQRYARPWLNEFRSWLQTMQTQTAPNSGLRKA
IDYTLKRWSALVCYLDDGGVPIDNNRAENAVRGVALGRKNWLFAGSLAAGQRAAMIMSLLETAKANGHEPWVWLRDVLSRLPVWPNNRLNELLPWPENPF
R

 

Blast result :
Comments
ISPlu20 is 68% (ORFC) aa similar to ISShdy2.
References
1] Friedhelm Pfeiffer (2016) Direct submission.
2] Duchaud,E., Rusniok,C., Frangeul,L., Buchrieser,C., Taourit,S., Bocs,S., Boursaux-Eude,C., Chandler,M., Dassa,E., Derose,R., Derzelle,S., Freyssinet,G., Gaudriault,S., Givaudan,A., Glaser,P., Medigue,C., Lanois,A., Powell,K., Siguier,P., Wingate,V., Zouine,M., Boemare,N., Danchin,A. and Kunst,F. (2003) Nat. Biotechnol. 11: 1307-1313