ISGmo1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AUDX01000033 | ND | Gulosibacter molinativorax | Gulosibacter molinativorax DSM 13485 |
DNA section
IS Length : 1287 bp
Ends
IR Length : 20/28
IRL : TGGGGTGTGACACTTGGGGTAGACAGTCGTTACCAGGAAGTTGAATGAAC
IRR : TGGGCTGTGTCAAGTTTTGTAGACACTCTTCTTGTGGATCAGGCCGCGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGGCAAGCG | CTAT | CGCGTTGCCC | 4 |
| GGCGAGCGCG | AGT | ATCAGCCAGC | 3 |
DNA sequence
TGGGGTGTGACACTTGGGGTAGACAGTCGTTACCAGGAAGTTGAATGAACGACATGACCATGAAGAAACCCCGCCGTCAGTTCACTGACGAGTTCAAGGC
CGACGCTGTGCAACTCGTCATCCAGGGACAGCGGCCGATCGCGCAGGTCGCCCGGGAGCTCGAGATTAACGAGTCTTCTCTGGGGTACTGGGTGAAGAAC
TACCGGCAAGCCAACCCGGACCCGCAGACGGCGCCTGCCCCGGTCGACGCCGCGCGATATGCGAGGCTCGAGGCGGAGAACCGTCGTCTGGCAGAGGAGA
ACGCGTTCCTGAAAAAAGCCGCGGCCTTCTTCGCGAAGGAACAGCGGTGAGCGTGAAGTTCCAGCTGATCCACGAGGAGAAGGCCCACCACACGATTGGC
CTGATGACACGCCTGCTGAAGGTGTCTCGGGCTGGCTACTACGCCTGGGCGAAACGACAAGGCACCACCACGCCATCTGGTCGCCGACGCGATGAGTTGG
CGGCGTTGATCAAGCAGATCGACGAGGACAAGCAGCAGACGTACGGGTTCCGGAGGATGCTCCACGAGCTTGCCCGCAGGGGTGTCACGGCCTCGGCGGG
CCTGGTGCGGAAGCTGATGCGCCAGCTCGGTGTGCACGGGGTGCAGCCGCGTGCGTCGAAGCGGACCACGATTCCCGCACTCGACGCCCAGGACCGTCCG
GACTGGTTGCGCCGCGACTTCACCGCCGAGCAGCCAGGGCAGCGGTTCGTCGGTGACATCACGTATCTGCGCACCGGGGAGGGATGGCTGTATCTCGCGA
CCGTGATTGATCTGTACAACCGTGAAGTGGTGGGGTGGTCGATGGCTGATCACATGCGCGTCGAGCTCGTCAGCGATGCCCTCACGATGGCGCATACCCA
CGGTCGTATCGGTGAGGGTGCCGTGTTTCACTCGGATCGCGGCTCGGTCTACACCTCGGCTGCCTATGCCGAGCTTGCCGAGCAGTGCCAGGTGAAGCTC
TCGGTTGGGCGGACCGGGGTGTGCTGGGACAACGCCGTCGCTGAGAGCTTCTTCTCGATGTTGAAGAACGAGATGTACCACCGGCAGGCTTTCGCCACCA
GAGGGCGTGCGAGGTTCGCGGTGATGGAGTACATCGAGGTGTTCTACAACCGTGGCAGGCTCCACTCAACGCTGGGATATCGCACCCCTGTCGAGGTCCG
CCACGCATACGAACGAAACACAGATCATCAGAACGCGGTCGCGGCCTGATCCACAAGAAGAGTGTCTACAAAACTTGACACAGCCCA
CGACGCTGTGCAACTCGTCATCCAGGGACAGCGGCCGATCGCGCAGGTCGCCCGGGAGCTCGAGATTAACGAGTCTTCTCTGGGGTACTGGGTGAAGAAC
TACCGGCAAGCCAACCCGGACCCGCAGACGGCGCCTGCCCCGGTCGACGCCGCGCGATATGCGAGGCTCGAGGCGGAGAACCGTCGTCTGGCAGAGGAGA
ACGCGTTCCTGAAAAAAGCCGCGGCCTTCTTCGCGAAGGAACAGCGGTGAGCGTGAAGTTCCAGCTGATCCACGAGGAGAAGGCCCACCACACGATTGGC
CTGATGACACGCCTGCTGAAGGTGTCTCGGGCTGGCTACTACGCCTGGGCGAAACGACAAGGCACCACCACGCCATCTGGTCGCCGACGCGATGAGTTGG
CGGCGTTGATCAAGCAGATCGACGAGGACAAGCAGCAGACGTACGGGTTCCGGAGGATGCTCCACGAGCTTGCCCGCAGGGGTGTCACGGCCTCGGCGGG
CCTGGTGCGGAAGCTGATGCGCCAGCTCGGTGTGCACGGGGTGCAGCCGCGTGCGTCGAAGCGGACCACGATTCCCGCACTCGACGCCCAGGACCGTCCG
GACTGGTTGCGCCGCGACTTCACCGCCGAGCAGCCAGGGCAGCGGTTCGTCGGTGACATCACGTATCTGCGCACCGGGGAGGGATGGCTGTATCTCGCGA
CCGTGATTGATCTGTACAACCGTGAAGTGGTGGGGTGGTCGATGGCTGATCACATGCGCGTCGAGCTCGTCAGCGATGCCCTCACGATGGCGCATACCCA
CGGTCGTATCGGTGAGGGTGCCGTGTTTCACTCGGATCGCGGCTCGGTCTACACCTCGGCTGCCTATGCCGAGCTTGCCGAGCAGTGCCAGGTGAAGCTC
TCGGTTGGGCGGACCGGGGTGTGCTGGGACAACGCCGTCGCTGAGAGCTTCTTCTCGATGTTGAAGAACGAGATGTACCACCGGCAGGCTTTCGCCACCA
GAGGGCGTGCGAGGTTCGCGGTGATGGAGTACATCGAGGTGTTCTACAACCGTGGCAGGCTCCACTCAACGCTGGGATATCGCACCCCTGTCGAGGTCCG
CCACGCATACGAACGAAACACAGATCATCAGAACGCGGTCGCGGCCTGATCCACAAGAAGAGTGTCTACAAAACTTGACACAGCCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 60 | 350 | + | No |
Description : First part of the transposase
ORF sequence :
MKKPRRQFTDEFKADAVQLVIQGQRPIAQVARELEINESSLGYWVKNYRQANPDPQTAPAPVDAARYARLEAENRRLAEENAFLKKAAAFFAKEQR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1080 bp | 359 aa | 170 | 1249 | + | No |
Description : Second part of the transposase
ORF sequence :
RVFSGVLGEELPASQPGPADGACPGRRRAICEARGGEPSSGRGERVPEKSRGLLREGTAVSVKFQLIHEEKAHHTIGLMTRLLKVSRAGYYAWAKRQGTT
TPSGRRRDELAALIKQIDEDKQQTYGFRRMLHELARRGVTASAGLVRKLMRQLGVHGVQPRASKRTTIPALDAQDRPDWLRRDFTAEQPGQRFVGDITYL
RTGEGWLYLATVIDLYNREVVGWSMADHMRVELVSDALTMAHTHGRIGEGAVFHSDRGSVYTSAAYAELAEQCQVKLSVGRTGVCWDNAVAESFFSMLKN
EMYHRQAFATRGRARFAVMEYIEVFYNRGRLHSTLGYRTPVEVRHAYERNTDHQNAVAA
TPSGRRRDELAALIKQIDEDKQQTYGFRRMLHELARRGVTASAGLVRKLMRQLGVHGVQPRASKRTTIPALDAQDRPDWLRRDFTAEQPGQRFVGDITYL
RTGEGWLYLATVIDLYNREVVGWSMADHMRVELVSDALTMAHTHGRIGEGAVFHSDRGSVYTSAAYAELAEQCQVKLSVGRTGVCWDNAVAESFFSMLKN
EMYHRQAFATRGRARFAVMEYIEVFYNRGRLHSTLGYRTPVEVRHAYERNTDHQNAVAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 60 | 1249 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKPRRQFTDEFKADAVQLVIQGQRPIAQVARELEINESSLGYWVKNYRQANPDPQTAPAPVDAARYARLEAENRRLAEENAFLKKSRGLLREGTAVSVK
FQLIHEEKAHHTIGLMTRLLKVSRAGYYAWAKRQGTTTPSGRRRDELAALIKQIDEDKQQTYGFRRMLHELARRGVTASAGLVRKLMRQLGVHGVQPRAS
KRTTIPALDAQDRPDWLRRDFTAEQPGQRFVGDITYLRTGEGWLYLATVIDLYNREVVGWSMADHMRVELVSDALTMAHTHGRIGEGAVFHSDRGSVYTS
AAYAELAEQCQVKLSVGRTGVCWDNAVAESFFSMLKNEMYHRQAFATRGRARFAVMEYIEVFYNRGRLHSTLGYRTPVEVRHAYERNTDHQNAVAA
FQLIHEEKAHHTIGLMTRLLKVSRAGYYAWAKRQGTTTPSGRRRDELAALIKQIDEDKQQTYGFRRMLHELARRGVTASAGLVRKLMRQLGVHGVQPRAS
KRTTIPALDAQDRPDWLRRDFTAEQPGQRFVGDITYLRTGEGWLYLATVIDLYNREVVGWSMADHMRVELVSDALTMAHTHGRIGEGAVFHSDRGSVYTS
AAYAELAEQCQVKLSVGRTGVCWDNAVAESFFSMLKNEMYHRQAFATRGRARFAVMEYIEVFYNRGRLHSTLGYRTPVEVRHAYERNTDHQNAVAA
Blast result :
Comments
ISGmo1 is 63% aa similar to ISFsp8 (Frankia sp.). The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift. ISGmo1 is part of a composite transposon.
A mettre dans IS_wait
A mettre dans IS_wait
References
[1] Vaz M., Nunes O., and Egas C. (2013) Direct submission to ISfinder