ISfinder Mirror Site

ISVa9

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Vibrio anguillarum	Vibrio anguillarum VIB43 (O1)

DNA section

IS Length : 2372 bp

Ends

IR Length : 14/19

IRL : GTAAGCGCGTCATAAACCCCGCATTCTAATCACCTAGCCCGAATAATAAG
IRR : GTATCCGCCCCATGATCCCACGGGGCGATATTGTTAGTGTTGGAAGTTCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACCGGTACTG	AATGCATA	CTTACTAATG	8
ATGACATGTC	GGTATCGC	TAGATGAAAT	8
AAAGCAGCTC	AGAAGTCG	GCATGCCTGC	8
GAGGGACTTC	TGTATAAA	AGACAGCCAG	8
CGGACTCAGT	GCTTGAGC	AAGTGATTGA	8
CTGTTCAGGG		GATGAGACGA	0
CCTGTCTGTG		TAATAAAGTT	0
CGGCCGCTTC		TTAGTATGCG	0
ATACTATTGC		TTTTGTTTTA	0
CTGGTTCTTC		CATTTATCTA	0
GCGATCAAGG		GTTTCAAGTT	0
			0

DNA sequence

GTAAGCGCGTCATAAACCCCACATTCTAATCAATCTCGGCACGCCATAAGATCAAATCTCCAACCTCAAGGAGATTTGAAATGGCAAAAAGACTCTCTCA
TCAAGAATGGCATACCCTATTCGATCAATACGAATCCAGTCATCTGTCTCAACGCGTGTTCTGTGAACAAAATGGGCTGAGCCTTTCAACCTTCCATGCC
AAGCGACAGCAACTAAAGTTCATAGAGCAACCTAAGAGCAGTGGATTCGTTAAAGCTGAAGTCGTTGAAAAAACGACACGCTACCAGATGACTCAAACAC
CGATTGCCAACATGACACTATTGATTAATGACGTTGAACTCAGCATCCCCCAGGGAACACCTGCCTCGTATCTGGCAGAGTTAATCGGAGCACTGTCATG
AAGAGAATGCTGAGTGCCCCTAATGTTTACCTGTATCGGGAGTTCGTGGATTTTAGAAAGTCCATTAATGGTCTGGCGGCGATTATTGAATCTGAAACCG
ATTTACCGCTCGGAAGCGGTGCGCTGTTCCTGTTTACCAATAAACAACGCGATAAAGTCAAAGTCTTGTACTGGGATAAAACGGGCTTCGCTCTGTGGTA
TAAACGGTTGGAAAAAGCCAAATTCAAATGGCCCACTCAAGAGAAAAAGCAGGTATACACCCTGACTCAATTTGACCTAGACAGACTGTTATCTGGCTTC
ACGATAATCGGCCATAAACCGATAAAAATAGACAACTTTACAATGAGTTGAACCCGATAAAAGTGAGTTATACCAAGCCTTGACGTGGTGTTTTTAATAT
AATCAATGCCATGAAAAAGACGCCCGATATTAACCCAGAAAGCCAAGACATCGCCGAGCTTCAAGCTATGGTGAAAGCGTTGATGTCGGAGAAAAAAGAG
TGGCAACAAGAGCGCCAATCCTTAATTGAGCAGTTTAAACTGGCCCTTGACCGTCAATTCGCCAAACGCTCGGAAGCGTTAAAGCCGTATAACGAAGCAC
AGGGTGACCTCTTTAATGAGGCGGAATGTGAAGCGGCGAAATCGGAAGATGAGGTTATCACCACGACGACAATCAAACGTCGTGGTAAACGTCAACCGCT
TCCTAAAGACTTGCCACGTGAAATTATCGAGCTTGACCTAGATGAGCACGACAAACAGTGCTCATGCTGCCAACACATTTTGCATAAAATCGGCGAAGAC
CGCAGTGAAAAGCTTGAGTTCACGCCCGCCGTCCTAAAAGTGTTGGAATATGTTCGCCCGAAATACGCCTGTCGTCAGTGTGAACAAGAGGCTGAAAAAA
GCCACATTCACCAACAACCCGCACCGATAAGTATCATCCCCAAAAGCTTCGCTACCGAAAGTCTGCTTGCCAACATTATTTTAGGCAAATATCAGTATGC
GCTTCCGCTGTATCGTCAGGAAACCTTGTTCACCCAATCAGGTATCGAGTTATCGAGAACGACCATGGCCAGGTGGGTAATCCAGGTTAGTGAAAAGTTC
AAACCGCTTTATCAGGCACTGAAGACTCACTTACTCGAACAAGTGGTGGTTCAGGCCGATGAAACGCCGCTCAATGTGCTCAAGGAAGACAAGCAATGTT
ATATGTGGTTGTACTGCTCGGGAGCCGACTCCCCTGAAGCTAAACTCCCTGACATGAAAAATATCGTCTTGTTCGACTATCAAAACAGTCGCGCGAGAGT
GTGCCCCGTGAACTTCTTGGGTGATTACTCAGGCTATCTGCAAAGTGATGGTTACGCGGCTTATGATGGATTAACCAACGTCACCAATGTCGGTTGCTTC
GCTCATGCACGTCGTAAGTTCATGGAAGCGAAGAAGCTTCAGGGCAAAGGTAAAACAGGTAAAGCGGATGTGGCGTTAGCCAAAATCCAAAAGTTGTACG
CACTTGAATCTCGATTAAAACTGGCTTCGGTCGAAGAGCGTTGGGCAGAAAGACAAGCGCAGGCTAAACCGATGTTGGATGACATCTATGACTGGTTAAC
CACGCAGAAAGTCATCGAATCGAGTCCTCTTGGTAAAGCCATCAAGTACACCTTGGGTCAGTGGCCTAAGCTGATACGCTATGTTGAAGATGGGCATTTA
TCTATCGACAACAACCGAGCGGAACGCGCAATCAAATCGCTGGTCATTGGCAGAAAAAATTGGTTATTCTCGACGAATCCGCTTGGTGCTGATGCGAGTG
CTTTGCTGTATAGCATCATCGAGACGGCGAAAGCGAACGGACTGATACTCTACGATTACATGGTCAAATGTATGAAAGAGCTGGCAAAGTCTGAGCCTGA
TATCAACTCACTGCTTCCCTGGAACTTCTCACATTCAAATTAAAACGCCCCGTGGGATCATGGGGCGCATAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
321 bp	106 aa	81	401	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAKRLSHQEWHTLFDQYESSHLSQRVFCEQNGLSLSTFHAKRQQLKFIEQPKSSGFVKAEVVEKTTRYQMTQTPIANMTLLINDVELSIPQGTPASYLAE
LIGALS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	398	751	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MKRMLSAPNVYLYREFVDFRKSINGLAAIIESETDLPLGSGALFLFTNKQRDKVKVLYWDKTGFALWYKRLEKAKFKWPTQEKKQVYTLTQFDLDRLLSG
FTIIGHKPIKIDNFTMS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1533 bp	510 aa	811	2343	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKTPDINPESQDIAELQAMVKALMSEKKEWQQERQSLIEQFKLALDRQFAKRSEALKPYNEAQGDLFNEAECEAAKSEDEVITTTTIKRRGKRQPLPKD
LPREIIELDLDEHDKQCSCCQHILHKIGEDRSEKLEFTPAVLKVLEYVRPKYACRQCEQEAEKSHIHQQPAPISIIPKSFATESLLANIILGKYQYALPL
YRQETLFTQSGIELSRTTMARWVIQVSEKFKPLYQALKTHLLEQVVVQADETPLNVLKEDKQCYMWLYCSGADSPEAKLPDMKNIVLFDYQNSRARVCPV
NFLGDYSGYLQSDGYAAYDGLTNVTNVGCFAHARRKFMEAKKLQGKGKTGKADVALAKIQKLYALESRLKLASVEERWAERQAQAKPMLDDIYDWLTTQK
VIESSPLGKAIKYTLGQWPKLIRYVEDGHLSIDNNRAERAIKSLVIGRKNWLFSTNPLGADASALLYSIIETAKANGLILYDYMVKCMKELAKSEPDINS
LLPWNFSHSN

Blast result :

Comments

ISVa9 is 85% (orfA), 97% (orfB) and 90% (orfC, the transposase) aa similar to ISVa5.

References

1] Holm K.O. (2016) Direct submisison to ISfinder
2] Frans I. (2013) PLoS One. Aug 6;8(8):e70477. doi: 10.1371