ISRsp20
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AEYF01000045 | ND | Rhizobium sp. | Rhizobium sp. CCGE 510 plasmid pRspCCGE510d |
DNA section
IS Length : 1559 bp
Ends
IR Length : 16/22
IRL : GAGCCTGTGAATGTTTGGTGGCGGCGGCGATTGGATTGTGATTCTGTTGG
IRR : GAGCTTGTGAAGCTTTTGAAGCTGGTGCGTTTCTGGCCGATTTTGGGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTTCGGCCTGCGCGCC | TCA | GGTTGAGGGATGCCGGGA | 3 |
DNA sequence
GAGCCTGTGAATGTTTGGTGGCGGCGGCGATTGGATTGTGATTCTGTTGGGTGATTGATTTGCTTGGGGGATGATCGTGAGCGACGAGCTGTTCGAAGGA
TTGCCGGTGCATGGATCGTGGCGCAGTGCTCAGGTGGGGCGCGGGGCGGCGCGCATGCGCGAGCCGGTGCGCGATCAGATCGAGTTGCGAGCCGTGGATA
TCGACAGCTTGATTGGCCAGGATCACGCAGTGCGCGTTATTTGGCGCTATGTCGAGGGACTGGATCTGAGCGCGCTTGAAGATCGGATCAAAGCGCGTGA
GCACCGGCCGGGTCATCCGCCGATTTCGCCACGGCTTTTGCTGGCGCTGTGGCTCTATGCCAGCAGCGACGGCGTCGGCAGCGCGCGGGCGCTGGAACGG
CTGTGCGGGAGCCACGACGTCTATCGCTGGCTGTGTGGCGGTGTCTCGGTGAACTATCACACGCTGGCGGATTTTCGGGTTGGTTGCGCCGATTTGCTCG
ACCGACTGCTGGCCGAGCATCTGGCGGCGCTTGCCGATGCCGGCCTCGTCGTTCTCGATAGTCTGGCGCAGGATGGCGTGCGGGTCCGGGCGAGCGCGGG
GGCCGCCTCGTTCGGCCGCAAGGCGACGCTTGATCGGCATCTTTCCATTGCCGAGGCGGTTGTGGATCAGCTCAAGCACGAGGTCGATGCGCGTTCGGAT
GCCAGCACTCGGCGCATCGAGGCGGCCAGGGAACGAGCGGCGCGTGAGCGCGGCGAGCGGCTCAGAGCAGCCCAGGTGGCCCTTGACGAGATCCAGCGCC
ATCGCCAGGCGCGCGAAGAAAAGCGCGGCAATGGCAAAAAGCCGAAGGAGCCTCGCGCCTCCAGCACAGATGCACAGGCGCGGGTGATGAAGATGGCCGA
CGGCGGCTTCCGTCCGGGTTATAATGTGCAGGTTGCGAGCACGGCCGGTGAGCAGTTCGTGGTCGGGCTCGAAGTGACCAATGCCGGCTCCGACCGCGGC
CTCATGCGGCCGATGCTGGAGCGGTTACGCGCGCTGAGCGGCCATCTGCCGCGACGTTATCTCGCCGATGGCGGCTTTTGCAGCGCTGAAGATATCGAAT
GGGCGCACGGCGAAGGGGTTGAAGTCTTTTGTCCGCCCACCCATTCCAAGCACGGAACCGATCCCTTCTTACCGCGGCGCGGCGACGGTCCGGGTGTATC
AGCCTGGCGGGCCCGCATGGGAAGCGAGGCGGGCAAGGCTCAGTACAAACCCCGCTCGATCTGCGAATGCATCCATGCCCGCTGGCGCAACTGGAATCTG
CGACAATTGACGGTGCGCGGCATCGAAAAGGTCCAGGCCGTCGTGCTCTGCTACGCACTCACCAACAATATCTTGCAAGGCCATCGGCTTGCCAATGGCT
AAACCACTGCAATCACCCAGACACCAACACGCGACCACCCGAACGCACCTCGCAATCGGCTAACCCTCGATGACATCGGCCAGCCAAGACTGGCCAAGAC
CAACCAAAACCGCCCAAAATCGGCCAGAAACGCACCAGCTTCAAAAGCTTCACAAGCTC
TTGCCGGTGCATGGATCGTGGCGCAGTGCTCAGGTGGGGCGCGGGGCGGCGCGCATGCGCGAGCCGGTGCGCGATCAGATCGAGTTGCGAGCCGTGGATA
TCGACAGCTTGATTGGCCAGGATCACGCAGTGCGCGTTATTTGGCGCTATGTCGAGGGACTGGATCTGAGCGCGCTTGAAGATCGGATCAAAGCGCGTGA
GCACCGGCCGGGTCATCCGCCGATTTCGCCACGGCTTTTGCTGGCGCTGTGGCTCTATGCCAGCAGCGACGGCGTCGGCAGCGCGCGGGCGCTGGAACGG
CTGTGCGGGAGCCACGACGTCTATCGCTGGCTGTGTGGCGGTGTCTCGGTGAACTATCACACGCTGGCGGATTTTCGGGTTGGTTGCGCCGATTTGCTCG
ACCGACTGCTGGCCGAGCATCTGGCGGCGCTTGCCGATGCCGGCCTCGTCGTTCTCGATAGTCTGGCGCAGGATGGCGTGCGGGTCCGGGCGAGCGCGGG
GGCCGCCTCGTTCGGCCGCAAGGCGACGCTTGATCGGCATCTTTCCATTGCCGAGGCGGTTGTGGATCAGCTCAAGCACGAGGTCGATGCGCGTTCGGAT
GCCAGCACTCGGCGCATCGAGGCGGCCAGGGAACGAGCGGCGCGTGAGCGCGGCGAGCGGCTCAGAGCAGCCCAGGTGGCCCTTGACGAGATCCAGCGCC
ATCGCCAGGCGCGCGAAGAAAAGCGCGGCAATGGCAAAAAGCCGAAGGAGCCTCGCGCCTCCAGCACAGATGCACAGGCGCGGGTGATGAAGATGGCCGA
CGGCGGCTTCCGTCCGGGTTATAATGTGCAGGTTGCGAGCACGGCCGGTGAGCAGTTCGTGGTCGGGCTCGAAGTGACCAATGCCGGCTCCGACCGCGGC
CTCATGCGGCCGATGCTGGAGCGGTTACGCGCGCTGAGCGGCCATCTGCCGCGACGTTATCTCGCCGATGGCGGCTTTTGCAGCGCTGAAGATATCGAAT
GGGCGCACGGCGAAGGGGTTGAAGTCTTTTGTCCGCCCACCCATTCCAAGCACGGAACCGATCCCTTCTTACCGCGGCGCGGCGACGGTCCGGGTGTATC
AGCCTGGCGGGCCCGCATGGGAAGCGAGGCGGGCAAGGCTCAGTACAAACCCCGCTCGATCTGCGAATGCATCCATGCCCGCTGGCGCAACTGGAATCTG
CGACAATTGACGGTGCGCGGCATCGAAAAGGTCCAGGCCGTCGTGCTCTGCTACGCACTCACCAACAATATCTTGCAAGGCCATCGGCTTGCCAATGGCT
AAACCACTGCAATCACCCAGACACCAACACGCGACCACCCGAACGCACCTCGCAATCGGCTAACCCTCGATGACATCGGCCAGCCAAGACTGGCCAAGAC
CAACCAAAACCGCCCAAAATCGGCCAGAAACGCACCAGCTTCAAAAGCTTCACAAGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1332 bp | 443 aa | 71 | 1402 | + | No |
Chemistry : DDE
ORF sequence :
MIVSDELFEGLPVHGSWRSAQVGRGAARMREPVRDQIELRAVDIDSLIGQDHAVRVIWRYVEGLDLSALEDRIKAREHRPGHPPISPRLLLALWLYASSD
GVGSARALERLCGSHDVYRWLCGGVSVNYHTLADFRVGCADLLDRLLAEHLAALADAGLVVLDSLAQDGVRVRASAGAASFGRKATLDRHLSIAEAVVDQ
LKHEVDARSDASTRRIEAARERAARERGERLRAAQVALDEIQRHRQAREEKRGNGKKPKEPRASSTDAQARVMKMADGGFRPGYNVQVASTAGEQFVVGL
EVTNAGSDRGLMRPMLERLRALSGHLPRRYLADGGFCSAEDIEWAHGEGVEVFCPPTHSKHGTDPFLPRRGDGPGVSAWRARMGSEAGKAQYKPRSICEC
IHARWRNWNLRQLTVRGIEKVQAVVLCYALTNNILQGHRLANG
GVGSARALERLCGSHDVYRWLCGGVSVNYHTLADFRVGCADLLDRLLAEHLAALADAGLVVLDSLAQDGVRVRASAGAASFGRKATLDRHLSIAEAVVDQ
LKHEVDARSDASTRRIEAARERAARERGERLRAAQVALDEIQRHRQAREEKRGNGKKPKEPRASSTDAQARVMKMADGGFRPGYNVQVASTAGEQFVVGL
EVTNAGSDRGLMRPMLERLRALSGHLPRRYLADGGFCSAEDIEWAHGEGVEVFCPPTHSKHGTDPFLPRRGDGPGVSAWRARMGSEAGKAQYKPRSICEC
IHARWRNWNLRQLTVRGIEKVQAVVLCYALTNNILQGHRLANG
Blast result :
Comments
ISRsp20 is 85% aa similar to ISBra1.
References
1] ISfinder annotation (2016)
2] Servin-Garciduenas,L.E., Rogel,M.A., Ormeno-Orrillo,E., Delgado-Salinas,A., Martinez-Romero,J., Sanchez,F. and Martinez-Romero,E. (2012) J. Bacteriol. 194 (22), 6310-6311.
2] Servin-Garciduenas,L.E., Rogel,M.A., Ormeno-Orrillo,E., Delgado-Salinas,A., Martinez-Romero,J., Sanchez,F. and Martinez-Romero,E. (2012) J. Bacteriol. 194 (22), 6310-6311.