ISfinder Mirror Site

ISEcl6

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Enterobacter cloacae	Enterobacter cloacae

DNA section

IS Length : 2498 bp

Ends

IR Length : 19/24

IRL : GTAAGCGTAAACCCAGTGCCGTATGTAGCCATTAAGCCAGTACTGATAAC
IRR : GTAAGCGTTAAGTGAGCGCCGTATTGACGGCTATTTATTGGTGAGATCTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
			0

DNA sequence

GTAAGCGTAAACCCAGTGCCGTATGTAGCCATTAAGCCAGTACTGATAACTTAGACGTCCACCTGTACAGACGGACATCTAAGTATGGAATCCCTATCCT
GGCGAAAAGAACCCCGTAAAAATTATCCCAATGACTTCAAGCTACGTATGGTCGAACTTGCTTCCCGCCCCGGAGCCTGCGTTGCTCAGATTGCCCGCGA
AAACGGCATCAATGATAACGTCCTCTTCAAATGGCTGCGGCTCTGGCAGAATGAAGGCCGTATATCACGCCGCCTTCCGGCAGCTGTCGAATCTCCATCA
TCGCCGATGATGCTGCCTGTCGAGGTGATACCTGATCCAGAACCAACAGGTGAAACAAACACTGACCCGGGTCAGTTCTCAGCGCTGAATGCCACCTCCT
GTTGTGTTGAGTTCCGTCACGGCAAAATGACGCTTGAGAACCCGTCACCAGAACTGTTGGCCGTGCTGCTCCGCGAGCTGACCGGGAGAAGCCAATGATA
TCCCTCCCGTCGGGCACCCGTATCTGGCTGGTTGCCGGCGTCACCGATATGCGTAAATCGTTCAATGGTCTGGGCGAGCTGGTACAGCATGCACTTGATG
AGGATCCGTTCTCCGGCCATCTGTTTATCTTCCGTGGCCGCAAGGGTGACACCGTGAAGATCCTCTGGGCTGATGCTGATGGCCTGTGCCTGTTTACCAA
ACGGCTGGAAGAAGGCCAGTTCATCTGGCCTGCTGTACGCGATGGCAAAATAGCGATCACCCGCTCACAACTTGCCATGCTCCTCGATAAGCTGGACTGG
CGTCAGCCAAAAACAACACGCCTTAACTCACTGACAATGTTGTAAAAAGGACATGGCCGCATTATAAATGAGGTCATGAGTCAGGACTATCTCGCCCGTA
TCGCCGCGCTGGAAGACGCGCTTCGCCAGAAAGACAGTCAGCTCAGCCTCGTTGCAGAGACTGAGTCGTTCCTGCGTTCGGCGCTGGCCCGCGCTGAAGA
GAAAATAGAGAACGAAGAGCGCGAAATAGAGCACCTGCGGGCACAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGCTCAGAAAAGCTACGCCGA
CAGGTTGAAGAAGCCGAAGCCCTGCTGAAACAGCAGGAGCAGCAAAGCGATCGCTACAACGGACGGGATAATGACCCACTGGTTACGCGTCAGCTTCGCC
AGTCACGCCACCGCCGACCACTCCCTGAACATCTTCCCCGTGAGATACACCGGCTGGAGCCATCTGAAACGGGTTGCCCGGAATGCGGTGGTGATATGGA
GTACCTCAGTGAAGTCAGCGCGGAGCAGCTGGAGCTGGTCTCCAGCGCCCTGAAAGTGATCCGCACGGTCAGAGTGAAAAAGGCCTGTGTCAGATGTGAC
TGTATCGTTGAAGCCCCTGCGCCATCACGTCCCATCGATCGCGGTATCGCAGGGCCGGGTCTGCTGGCCCGGGTGCTAACCGCGAAATACTGCGAACATC
TGCCGCTGTATCGTCAGACTGAAATCTTTGCCCGTCAGGGGGTGGTTCTGAGTCGTGCGCTGCTCTCCAACTGGGTGGATGCCTGTTGCCGGTTAATGGC
CCCGCTGGATGAAGCCCTTTACCACTACGTGATGGACTGCCGCAAACTGCACACTGATGACACACCTGTTCCGGTGCTGGCGCCGGGCAGAAAGAAGACG
AAAACCGGGCGCATCTGGACGTATGTTCGCGATGACCGAAGCGCAGGCTCGTCAGATCCGCCAGCGGCGTGGTTCGCCTTCTCACCGGACAGGCAGGGGA
AGCATCCTCAGCAGCATCTTCGCCATTATCATGGTGTTCTGCAGGCGGATGCCTTCGCGGGTTATGATCGGTTGTTCAGTCCGGAGCGTGAAGGAGGGCC
ACTTACAGAAGCCGCATGCTGGGCCCATGCCCGAAGAAAAATCCATGACGTCTATATAAGCACGCAAACAGCGACAGCAGAAGAAGCCCTGAAACGTATC
AGCGAGCTGTACGCAATCGAAGAAGCAATACGCGGCCTCGCGGCAACTGAGCGGCTGGCAGCCAGGCAGTCCCGAAGTAAACCGCTACTGATATCCCTGC
ATGACTGGTTGGTGGAGAAAAGCGCCGCCCTGTCGAAAAAATCCCGGCTAGGCGAGGCGTTCGCTTATGTTCTGAACCAGTGGGATGCCCTGTGCTACTA
CTGCGATGACGGTCTGGCAGAGCCTGATAACAATGCAGCGGAGCGGGCGTTACGGGCAGTATGTCTGGGTAAAAAGAACTATATCTTCTTCGGCAGCGAC
CATGGTGGTGAACGTGGTGCCCTGCTGTATGGCCTAATCGGAACGTGCAGACTGAATGGCATCGATCCGGAAGCCTACTTCAGATATATCCTGAGCGTGC
TGCCGGAGTGGCCATCGAACAAAGTGGCAGAGCTTCTGCCATGGAACGTAGATCTCACCAATAAATAGCCGTCAATACGGCGCTCACTTAACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
414 bp	137 aa	85	498	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MESLSWRKEPRKNYPNDFKLRMVELASRPGACVAQIARENGINDNVLFKWLRLWQNEGRISRRLPAAVESPSSPMMLPVEVIPDPEPTGETNTDPGQFSA
LNATSCCVEFRHGKMTLENPSPELLAVLLRELTGRSQ

Blast result :ISEcl6 is 77% aa similar to ISEc23.

ORF 2

Length		Begin	End	Strand	Fusion ORF
351 bp	116 aa	495	845	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISLPSGTRIWLVAGVTDMRKSFNGLGELVQHALDEDPFSGHLFIFRGRKGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKIAITRSQLAMLLDKL
DWRQPKTTRLNSLTML

Blast result :ISEcl6 is 98% aa similar to IS682.

ORF 3

Length		Begin	End	Strand	Fusion ORF
1 bp	0 aa	0	0	-	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQDYLARIAALEDALRQKDSQLSLVAETESFLRSALARAEEKIENEEREIEHLRAQIEKLRRMLFGTRSEKLRRQVEEAEALLKQQEQQSDRYNGRDND
PLVTRQLRQSRHRRPLPEHLPREIHRLEPSETGCPECGGDMEYLSEVSAEQLELVSSALKVIRTVRVKKACVRCDCIVEAPAPSRPIDRGIAGPGLLARV
LTAKYCEHLPLYRQTEIFARQGVVLSRALLSNWVDACCRLMAPLDEALYHYVMDCRKLHTDDTPVPVLAPGRKKTKTGRIWTYVRDDRSAGSSDPPAAWF
AFSPDRQGKHPQQHLRHYHGVLQADAFAGYDRLFSPEREGGPLTEAACWAHARRKIHDVYISTQTATAEEALKRISELYAIEEAIRGLAATERLAARQSR
SKPLLISLHDWLVEKSAALSKKSRLGEAFAYVLNQWDALCYYCDDGLAEPDNNAAERALRAVCLGKKNYIFFGSDHGGERGALLYGLIGTCRLNGIDPEA
YFRYILSVLPEWPSNKVAELLPWNVDLTNK

Blast result :ISEcl6 is 95% aa similar to ISKox1

Comments

ISEcl6 is 75% (orfA), 95% (orfB) and 95% (orfC, the transposase) aa similar to ISKox1.

References

[1] Zhou D. (2016) Direct submission