ISAzch1

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP010415 ND Azotobacter chroococcum
Azotobacter chroococcum NCIMB 8003
DNA section
IS Length : 1584 bp

Ends


IR Length : 10/12

IRL : GAGCTTGTGAAAAAAACCACCGGGTCCCGCGCGCTGCGAGAATAGGCGCG
IRR : GAGGCTGTGAAACTTTCGATTCTGGAACCTGCTGGGCCGTGACACATTGG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCTTGTAATTGGAGGCTGGAGAGCTGAACGATCCATCGCGTGAACTGACTCA3

DNA sequence

GAGCTTGTGAAAAAAACCACCGGGTCCCGCGCGCTGCGAGAATAGGCGCGGGATTTGGACCGGAGGTAAGAGATGTCAGGCTCAAGTGGCGAGTTGTTCG
ACGATCTACCTATTCCAGAGGCACGACCCGCGCCGCCCCTCAAGGCGGCGGCGCGTGTGCGGCACCCGAATCGGGATCAGATCGAGCTGCGCCCCAGCGA
CCTGGAGAGTCTGCTTTCGGAGGATCATCCGGCACGGCTGGTGTGGGGCTACGTCGAGCGGCAGGACCTTTCGCCGCTATACGCCGGGATCAAGGCGGTG
GATGGCGCCTGCGGGCGCAGCGCGATTGCCCCGGAGATTCTGCTGGCACTGTGGCTGTATGCGACGCTGGAGGGCGTCGGCAGCGCGCGGGCGCTGGCAC
GGCTGTGCGAAGCGCATGATGCCTATCGCTGGCTGTGCGGCGGAGTACAGGTGAACCACCACACCCTGTCGGACTTTCGGGTAGGCCAGGGTGCCTTTCT
GGATCGCTTGCTGACCGCCAATGTGGCCAGCCTGCTGGCTACGGGTGCCGTGACCATGAAGCGCGTGGCACAGGATGGCATGCGCGTGCGGGCGCACGCT
GGCGCCGCCTCGTTCCGGCGCAAGGAACGGCTGCAGCAGTTCCACATGCAGGCGCGCCAGCAGGTCGAGGCCCTCAAGCGCGAAGTGCGGGACGATCCGG
CGGCCACCGAGCGACGCCAGCAGGCGGCGCGCGAGCGTGCCGCGCGGGAGCGCGAAGAGCGCATCGCCAAGGCGCTGGCCCAATTGCCGAAGGTCGAGAA
GATCAAGCAGGCACAAGGCAAGCCGGTCACCAGTGCCCGGGTCTCCACCACGGATGCCGAGGCCTGCGTGATGAAGATGCCCGACGGAGGTTTTCGCCCG
GCCTACAACATGCAGCTGGCCACCGATACGGCCAGCCAGGTGATCGTGGGTGTGGATGTGGTGACCCGCGGCAGCGACCTGGGCCAACTGGCGCCGATGG
TCGAGCAGCTCGATGAGCGTTACGAGCGCCGCCCGCAGGAGATGCTGGTGGACGGCGGCTTCGCCAAGCATGACGACATCGAGCGCCTGGCACCGACCAC
CACCGTCTATGCCCCGCCGCCCAAGCCCAGGGACGCCACGCGCGATCCTCATGCCGCCCTCCCGGACGACAGCGAGACCATCGCCGCCTGGCGGGAGCGG
ATGGGTACAGAGGCTGCCAAGGCGATCTACAAGGAGCGCGCCGCCACTGCCGAATGCGTCAACGCCCTGGCCCGCAATCGCGGTTTACAGCGTTTCAACG
TGTGCGGATTGGACAAGGTCAAAAGCGTGCTGTTGTGGTACGCGCTGGCACATAACCTGATGCGCATGCTAAAGCTTGCGCCTGGGTTGCTGGGTGTGCC
GACGATGACCTGAGGGAGCCGCGGACTGCGGCGATCAGGACAAAACGCCCCGCCGCACGCCTCTGCATCACTCCCATCGCCGCCCGTGCACTGCAAAAGC
GCGCGATGCAGAAAATCCCGACATCGCGCTGCCTCCAATGTGTCACGGCCCAGCAGGTTCCAGAATCGAAAGTTTCACAGCCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1341 bp446 aa731413+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSGSSGELFDDLPIPEARPAPPLKAAARVRHPNRDQIELRPSDLESLLSEDHPARLVWGYVERQDLSPLYAGIKAVDGACGRSAIAPEILLALWLYATLE
GVGSARALARLCEAHDAYRWLCGGVQVNHHTLSDFRVGQGAFLDRLLTANVASLLATGAVTMKRVAQDGMRVRAHAGAASFRRKERLQQFHMQARQQVEA
LKREVRDDPAATERRQQAARERAAREREERIAKALAQLPKVEKIKQAQGKPVTSARVSTTDAEACVMKMPDGGFRPAYNMQLATDTASQVIVGVDVVTRG
SDLGQLAPMVEQLDERYERRPQEMLVDGGFAKHDDIERLAPTTTVYAPPPKPRDATRDPHAALPDDSETIAAWRERMGTEAAKAIYKERAATAECVNALA
RNRGLQRFNVCGLDKVKSVLLWYALAHNLMRMLKLAPGLLGVPTMT

 

Blast result :
Comments
ISAzch1 is 67% aa similar to ISAde2.
References
1] ISfinder annotation (2016)
2] Robson,R.L., Jones,R., Robson,R.M., Schwartz,A. and Richardson,T.H. (2015) PLoS ONE 10 (6), E0127997.