ISVa8
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Vibrio anguillarum | Vibrio anguillarum Vibrio anguillarum VIB12 (O2) Vibrio parahaemolyticus (UCM-V493) Vibrio alginolyticus (st |
DNA section
IS Length : 1563 bp
Ends
IR Length : 16
IRL : GTAACCGTTCACCAGTCCCCCATTGACAATCAAGTGTGACACTCCGCACT
IRR : GTAACCGTTCACCAGTAGGAGCTTGATTTAATGGATTACAGACTAAATAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCCGCAATC | ACTGAAGG | ACCCGCGATC | 8 |
| CCATTCGATA | AAGCAAAT | CTCTCTCTCA | 8 |
| TAAGTCAGTA | GTCTATTT | TGTCGTCTTT | 8 |
| TTTCAGGATT | CAAATAAG | AAATGAGAGA | 8 |
| GTTGCAGGTA | AACGAATG | TATCCAACAC | 8 |
| AAATGTTATG | GCTAAAAC | GACTCGGCAT | 8 |
| TCTGTTTGTT | GGCTAAGC | TTCTTCGTCG | 8 |
| ACAAAGTATC | AAGCCACT | CACGTTGTCG | 8 |
| CCAACTCTAT | GAAACTGG | CGTTTCTGAT | 8 |
| CAGAGCTATT | CATTGCCA | TATCAATGAT | 8 |
DNA sequence
GTAACCGTTCACCAGTCCCCCATTGACAATCAAGTGTGACACTCCGCACTTGATCCACTTTTTCTTCTTGAGTGATCATTTACAGCCTTCATGATGAAGG
TATGAAAAAGAAGAAATCCAAATTCGCAGAAGCTCCTCCTGTTGCCTCTGATATCAATGAAGCTAACGCACTCATCGAAGAGTTGTGGGAACAACTTCGC
CATTATGAAGATAAGCTGAAAACCAGTTGCTCTAACTCTTCAAAACCACCGTCTTCGGATGGCCCAAAAGAGCGGGCTGAGCGAAAAAAGCTCAAAGCTC
TGGTGACCGCAATCCGAGAGGAGCTAAACAGGGTCACTCAGGGAGTCAACGACAACTCTCGAAACTAAAGAAAACGGACACTGTCATCGACTGCTTGCCT
GATCCTACGTGTCCGTGTTGTGGTCAATCGGACATCTTCGTTCACAATAACCCGTTTTATCGACATCAAGTTCATGAAATACCAAAGCCAGCGGTCGATA
TCTCTGAGTACCGATTATTTTCAGGCCAATGTCGACATTGCAATGAGGTTGTCAGAGGCAAGAAACCTGAGAATGTCTCACAAGGTATTATTGGGCCGAA
CTTGCTCAGCTATATCGCTGTATTAGCAGGACAATATCATTTAAGTATCCGCAAAATTTGCTCTCTACTAAAAGAACAACTGGGTACGACGTTCTCTGTT
GGTGCCATCAGCGAAGCTCAGACTAAAGTGGCTTCAATGCTGACTCCTTTACATCATGCAATCCGAGATAGCATCCAAACAGCACCGCTAGTTCATGTAG
ATGAAACTTCCCACCCTCGTAATGGTGAAGAGAGCTTACGTTGGTGTTGGTTGGTCGCCAGTGAAGACCTTGTCTACGAAAAGATCTTGTTCTCTCGTTC
AACCCATTCTGCCAAGACCATGTTAGGTAAGAATTATAGTGGTCTTGTCGTCACTGACCAGTGCCCAAGCTATAACTGGCTAAAACCAGAACAACATCAG
CTGTGCTGGTCGCATGTGAGGCGAAATCTTCAGCAAATCGCAGATTATACTGGCGGCGGTTATACCGCTCTCGTTGGACAACGGCTAACACTGCTCGCCA
ATATGGTTTTTAGAATACGTCACCGTTTGGAAAATACAGAAATCCAGTACGAACAGTATATTCGCCGCATGAATCGACTTCGAAAATCCTTTGACCACTG
GCTGCAAAAAGGCAGTGGGATAGTCGTTCAGCGATACAAAGGTCGATGTAAAAAGCTACAATCTCATAGGCAAAGCTTGTGGTTGTTCTTAACCGATACC
TCAATACCACTCACCAACAACGAAGCAGAGCGGAGAATACGTGGCAGTGTCATCCAGCGTAAGATTAGCTTCGGAACGACATCTGATGCGGGCGACAAGT
TCAGGGGCCGTATGCATTCGCTTGTTGAGACCTGTAACAAACGAGGCATCTCGAGCCTGTCTGCTCTAATGGAAATCGTTCAAGCTGTTACCCTTCGCAA
GCCTTACCCAAACGTATTTAGTCTGTAATCCATTAAATCAAGCTCCTACTGGTGAACGGTTAC
TATGAAAAAGAAGAAATCCAAATTCGCAGAAGCTCCTCCTGTTGCCTCTGATATCAATGAAGCTAACGCACTCATCGAAGAGTTGTGGGAACAACTTCGC
CATTATGAAGATAAGCTGAAAACCAGTTGCTCTAACTCTTCAAAACCACCGTCTTCGGATGGCCCAAAAGAGCGGGCTGAGCGAAAAAAGCTCAAAGCTC
TGGTGACCGCAATCCGAGAGGAGCTAAACAGGGTCACTCAGGGAGTCAACGACAACTCTCGAAACTAAAGAAAACGGACACTGTCATCGACTGCTTGCCT
GATCCTACGTGTCCGTGTTGTGGTCAATCGGACATCTTCGTTCACAATAACCCGTTTTATCGACATCAAGTTCATGAAATACCAAAGCCAGCGGTCGATA
TCTCTGAGTACCGATTATTTTCAGGCCAATGTCGACATTGCAATGAGGTTGTCAGAGGCAAGAAACCTGAGAATGTCTCACAAGGTATTATTGGGCCGAA
CTTGCTCAGCTATATCGCTGTATTAGCAGGACAATATCATTTAAGTATCCGCAAAATTTGCTCTCTACTAAAAGAACAACTGGGTACGACGTTCTCTGTT
GGTGCCATCAGCGAAGCTCAGACTAAAGTGGCTTCAATGCTGACTCCTTTACATCATGCAATCCGAGATAGCATCCAAACAGCACCGCTAGTTCATGTAG
ATGAAACTTCCCACCCTCGTAATGGTGAAGAGAGCTTACGTTGGTGTTGGTTGGTCGCCAGTGAAGACCTTGTCTACGAAAAGATCTTGTTCTCTCGTTC
AACCCATTCTGCCAAGACCATGTTAGGTAAGAATTATAGTGGTCTTGTCGTCACTGACCAGTGCCCAAGCTATAACTGGCTAAAACCAGAACAACATCAG
CTGTGCTGGTCGCATGTGAGGCGAAATCTTCAGCAAATCGCAGATTATACTGGCGGCGGTTATACCGCTCTCGTTGGACAACGGCTAACACTGCTCGCCA
ATATGGTTTTTAGAATACGTCACCGTTTGGAAAATACAGAAATCCAGTACGAACAGTATATTCGCCGCATGAATCGACTTCGAAAATCCTTTGACCACTG
GCTGCAAAAAGGCAGTGGGATAGTCGTTCAGCGATACAAAGGTCGATGTAAAAAGCTACAATCTCATAGGCAAAGCTTGTGGTTGTTCTTAACCGATACC
TCAATACCACTCACCAACAACGAAGCAGAGCGGAGAATACGTGGCAGTGTCATCCAGCGTAAGATTAGCTTCGGAACGACATCTGATGCGGGCGACAAGT
TCAGGGGCCGTATGCATTCGCTTGTTGAGACCTGTAACAAACGAGGCATCTCGAGCCTGTCTGCTCTAATGGAAATCGTTCAAGCTGTTACCCTTCGCAA
GCCTTACCCAAACGTATTTAGTCTGTAATCCATTAAATCAAGCTCCTACTGGTGAACGGTTAC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 102 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MKKKKSKFAEAPPVASDINEANALIEELWEQLRHYEDKLKTSCSNSSKPPSSDGPKERAERKKLKALVTAIREELNRVTQGVNDNSRN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1248 bp | 415 aa | 281 | 1528 | + | No |
Description : Second part of the transposase
ORF sequence :
AKKAQSSGDRNPRGAKQGHSGSQRQLSKLKKTDTVIDCLPDPTCPCCGQSDIFVHNNPFYRHQVHEIPKPAVDISEYRLFSGQCRHCNEVVRGKKPENVS
QGIIGPNLLSYIAVLAGQYHLSIRKICSLLKEQLGTTFSVGAISEAQTKVASMLTPLHHAIRDSIQTAPLVHVDETSHPRNGEESLRWCWLVASEDLVYE
KILFSRSTHSAKTMLGKNYSGLVVTDQCPSYNWLKPEQHQLCWSHVRRNLQQIADYTGGGYTALVGQRLTLLANMVFRIRHRLENTEIQYEQYIRRMNRL
RKSFDHWLQKGSGIVVQRYKGRCKKLQSHRQSLWLFLTDTSIPLTNNEAERRIRGSVIQRKISFGTTSDAGDKFRGRMHSLVETCNKRGISSLSALMEIV
QAVTLRKPYPNVFSL
QGIIGPNLLSYIAVLAGQYHLSIRKICSLLKEQLGTTFSVGAISEAQTKVASMLTPLHHAIRDSIQTAPLVHVDETSHPRNGEESLRWCWLVASEDLVYE
KILFSRSTHSAKTMLGKNYSGLVVTDQCPSYNWLKPEQHQLCWSHVRRNLQQIADYTGGGYTALVGQRLTLLANMVFRIRHRLENTEIQYEQYIRRMNRL
RKSFDHWLQKGSGIVVQRYKGRCKKLQSHRQSLWLFLTDTSIPLTNNEAERRIRGSVIQRKISFGTTSDAGDKFRGRMHSLVETCNKRGISSLSALMEIV
QAVTLRKPYPNVFSL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1427 bp | 475 aa | 102 | 1528 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKKKSKFAEAPPVASDINEANALIEELWEQLRHYEDKLKTSCSNSSKPPSSDGPKERAERKKAQSSGDRNPRGAKQGHSGSQRQLSKLKKTDTVIDCLP
DPTCPCCGQSDIFVHNNPFYRHQVHEIPKPAVDISEYRLFSGQCRHCNEVVRGKKPENVSQGIIGPNLLSYIAVLAGQYHLSIRKICSLLKEQLGTTFSV
GAISEAQTKVASMLTPLHHAIRDSIQTAPLVHVDETSHPRNGEESLRWCWLVASEDLVYEKILFSRSTHSAKTMLGKNYSGLVVTDQCPSYNWLKPEQHQ
LCWSHVRRNLQQIADYTGGGYTALVGQRLTLLANMVFRIRHRLENTEIQYEQYIRRMNRLRKSFDHWLQKGSGIVVQRYKGRCKKLQSHRQSLWLFLTDT
SIPLTNNEAERRIRGSVIQRKISFGTTSDAGDKFRGRMHSLVETCNKRGISSLSALMEIVQAVTLRKPYPNVFSL
DPTCPCCGQSDIFVHNNPFYRHQVHEIPKPAVDISEYRLFSGQCRHCNEVVRGKKPENVSQGIIGPNLLSYIAVLAGQYHLSIRKICSLLKEQLGTTFSV
GAISEAQTKVASMLTPLHHAIRDSIQTAPLVHVDETSHPRNGEESLRWCWLVASEDLVYEKILFSRSTHSAKTMLGKNYSGLVVTDQCPSYNWLKPEQHQ
LCWSHVRRNLQQIADYTGGGYTALVGQRLTLLANMVFRIRHRLENTEIQYEQYIRRMNRLRKSFDHWLQKGSGIVVQRYKGRCKKLQSHRQSLWLFLTDT
SIPLTNNEAERRIRGSVIQRKISFGTTSDAGDKFRGRMHSLVETCNKRGISSLSALMEIVQAVTLRKPYPNVFSL
Blast result :
Comments
ISVa8 is 84% a similar to ISVch7.
The transposase is reconstructed in silico by possible -1 frameshift.
The transposase is reconstructed in silico by possible -1 frameshift.
References
1] Holm K.O. (2016) Direct submisison to ISfinder
2] Frans I. (2013) PLoS One. Aug 6;8(8):e70477. doi: 10.1371
2] Frans I. (2013) PLoS One. Aug 6;8(8):e70477. doi: 10.1371