ISArve1

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP002588 ND Archaeoglobus veneficus
Archaeoglobus veneficus SNP6
DNA section
IS Length : 995 bp

Ends


IR Length : 14/17

IRL : TGCTTTGTCGAACAAACAAGCATAGAATAATACACAATAAAAATTAAATT
IRR : TGCCTTGTCGAATAATCCCCAACTAAACTTCCCCATCCACTCCAACAACT

Insertion site


Left flankDirect repeatRight flankDR Length
TATGTCTGTCGAAGGATCAAGCTTATTA8
AGTGGGAGAAGCATCGATGAACTCGATT8
AGAAGTACGGCTTCGTACCTTCGTACCG8

DNA sequence

TGCTTTGTCGAACAAACAAGCATAGAATAATACACAATAAAAATTAAATTCCAATAAAACCTTTAACCAACCCATGCCAAAAATAAACTGGAAAGAATAC
AATGAAAAGCTTGTAAAGAGAGGTGAAATTTTATTCAGTACGGAGTTTATAGAGAACTGGAAGAGAGAGCTCGATTCAATGAACGAAAGAAAGAGAGGTC
ATCCTTACGAGTATCCTAATTCTTTCATGCAGTTTTTAGCATCAATCCGCTACTACTGCCATCTCGACTACAGAACTCTGGAAGGATTCTGCAGAAGCTT
ATCAAGAATTCTCGATATACCAGCTCCAGATCATTCAACTATCCACAAGAGAGCTCCAAGGTTGGATTTCACTCCTCCAGCCGTTAACTCTGAAGAAATA
GTTATAGCGGTTGATTCTTCGGGAATTAAAGTTCATAACAGGGGAGAGTGGATGAGAGAGAAATACAGGAGGAGAAGGGGATGGATAAAGATCCATTTCG
CTGTTGACGTTGAAACTAAGCAGGTTGTAGCTTACGAAGTTACAGACGAGCAGGTTCACGATAACAAGGTCTTCAAGGATCTCGTAGAAAAATCTGAAGA
AAGAGCAAAGGTAAGGAGAGTTCTTGCCGATAAAGCGTATGACAGCTACGAAAACTTCGAATTTCTTCAATCAAGGGATATAGATCCTGCCATTCCAGTA
AGGAAAGGAGCTATAGACATCTTAAAGCATCCAAGAAGTGAGGAGGCAAGGAAGCAGAAAGATTTTGACGGGTGGAAGGAAGAGAAGAAATACGGGTTGA
GGTGGGCAGTTGAGACTGCTTATTCTGTCTTTAAGAGGTGTTTTGGTGAGTTCGTTTCAGCAAGGAAGCAGGATTACATGGTTAAGGAGATTGCAGCGAA
GGTGTGGATATACAATTCGCTGAGGGCTGTTCTCTGCTGAAGGGGAGTTGTTGGAGTGGATGGGGAAGTTTAGTTGGGGATTATTCGACAAGGCA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
867 bp288 aa74940+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKINWKEYNEKLVKRGEILFSTEFIENWKRELDSMNERKRGHPYEYPNSFMQFLASIRYYCHLDYRTLEGFCRSLSRILDIPAPDHSTIHKRAPRLDFT
PPAVNSEEIVIAVDSSGIKVHNRGEWMREKYRRRRGWIKIHFAVDVETKQVVAYEVTDEQVHDNKVFKDLVEKSEERAKVRRVLADKAYDSYENFEFLQS
RDIDPAIPVRKGAIDILKHPRSEEARKQKDFDGWKEEKKYGLRWAVETAYSVFKRCFGEFVSARKQDYMVKEIAAKVWIYNSLRAVLC

 

Blast result :
Comments
ISArve1 is 61% aa similar to ISMth3.
References
1] ISfinder annotation (2016)
2] Lucas,S., Copeland,A., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Pagani,I., Ivanova,N., Mikhailova,N., Lu,M., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2011) Direct submission GenBank.