ISMye2
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JH597761 | ND | Metallosphaera yellowstonensis | Metallosphaera yellowstonensis MK1 |
DNA section
IS Length : 1050 bp
Ends
IR Length : 26/33
IRL : TGCTTTGTGGGCTACTTCAGTATTATACAAAAATTTATAACTAATATATC
IRR : TGCTTTGTGGACCTCTTCAGTTTATTTCATAAGCATAATATGATACACTG
Comments : The first nucleotide seems to be mutated in all copies, the typical ends for this subgroup is "GG" and not "TG".
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAACTTTAT | CCCATGGAG | TGCTTGGGCG | 9 |
| AATCACTCGT | CCTCATCAC | TCCTCTTCTC | 9 |
| ATCCTTTTTC | CCGTTAATA | GAAATCTTTT | 9 |
| CCGGTGGAGAGGGGAAAAGG | GATCTACTGTGAAGGGTGTG | 0 | |
| TAAACATTAC | TTAATTAAA | ACGATATTTA | 9 |
DNA sequence
TGCTTTGTGGGCTACTTCAGTATTATACAAAAATTTATAACTAATATATCGAGTATTACCCATGGGAAAGAGTGAGTACAAGAGGGATTGGAGCAAGTAC
GACGAGAACGTCATAACGAGATACAAGCTAATGTTCCCCTTCTACGTCTTCGAACACTGGTGGGATCTACTAGCAGAGGAGAATAGGGATGCAAAGAAAA
CCTACAAGGCACCGAAGGAGTTCAACGAGTTCCTAGCATTCCTACACTTGTTCTTACCCTACAGGGCAATGGAAGGGGTATTGAGAGCACTGGAGGGACT
GAAGATCGTCCCGACAAGCCTAGACTACTCGACGATGTGGGAAAGGGTGAGGAACATGGACATAACGTTCCCGGAGGCAAACGACCAACTTGAAGTAATA
GCAGACGCGACGGGGATAAGCACAACAAGGGGAGGACAATACATCATTGCAAAGTGGGGTAAAAGGAGGGACTCAAAATTCCTCAAGATAGAGATAGTAA
TGGATAAGGACGAGCTCAACGTGATAGACGCTGAGGTGACTAGCGACGAGGTCGAGACTGCAGTCAAGACTAAGGACTTACAGGATGAGGGGAAGAAGGT
CAAGAGGTTTTACGGGGATAAGGCTTACGACGCCAACGAGGTTTACAATACTGGGGTTGAGGTCGTCCCACCTGGGGAGGACGCCTCCACTGGACGCGGT
CATCCCGCCAGGAGAAAGGCTGTGAGGGAGTTCAAGGAGTTGGGTTACGATCGTTGGAGGGAGGAGAGGGGTTACGGTGTTAGGTGGAGGGTTGAGTCCC
TGTTCTCTGCCGTGAAGCGCACCTTTGGTGAATCTGTTGGGGCTACAAGTTTTCTAGGGCAAGTGGTTGAGGCTAAGCTCAAGTTCTGGGCCTACGCGTG
GATGGTTCACTTGGCTAATTCTTTAGTCGGTAGGGCTCCGGGTATTAAGGTTTGAGCTTGTGAACAACGTTGAAATAAATATTAATTACTGAAAAACCCT
CAGTGTATCATATTATGCTTATGAAATAAACTGAAGAGGTCCACAAAGCA
GACGAGAACGTCATAACGAGATACAAGCTAATGTTCCCCTTCTACGTCTTCGAACACTGGTGGGATCTACTAGCAGAGGAGAATAGGGATGCAAAGAAAA
CCTACAAGGCACCGAAGGAGTTCAACGAGTTCCTAGCATTCCTACACTTGTTCTTACCCTACAGGGCAATGGAAGGGGTATTGAGAGCACTGGAGGGACT
GAAGATCGTCCCGACAAGCCTAGACTACTCGACGATGTGGGAAAGGGTGAGGAACATGGACATAACGTTCCCGGAGGCAAACGACCAACTTGAAGTAATA
GCAGACGCGACGGGGATAAGCACAACAAGGGGAGGACAATACATCATTGCAAAGTGGGGTAAAAGGAGGGACTCAAAATTCCTCAAGATAGAGATAGTAA
TGGATAAGGACGAGCTCAACGTGATAGACGCTGAGGTGACTAGCGACGAGGTCGAGACTGCAGTCAAGACTAAGGACTTACAGGATGAGGGGAAGAAGGT
CAAGAGGTTTTACGGGGATAAGGCTTACGACGCCAACGAGGTTTACAATACTGGGGTTGAGGTCGTCCCACCTGGGGAGGACGCCTCCACTGGACGCGGT
CATCCCGCCAGGAGAAAGGCTGTGAGGGAGTTCAAGGAGTTGGGTTACGATCGTTGGAGGGAGGAGAGGGGTTACGGTGTTAGGTGGAGGGTTGAGTCCC
TGTTCTCTGCCGTGAAGCGCACCTTTGGTGAATCTGTTGGGGCTACAAGTTTTCTAGGGCAAGTGGTTGAGGCTAAGCTCAAGTTCTGGGCCTACGCGTG
GATGGTTCACTTGGCTAATTCTTTAGTCGGTAGGGCTCCGGGTATTAAGGTTTGAGCTTGTGAACAACGTTGAAATAAATATTAATTACTGAAAAACCCT
CAGTGTATCATATTATGCTTATGAAATAAACTGAAGAGGTCCACAAAGCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 894 bp | 297 aa | 62 | 955 | + | No |
Chemistry : DDE
ORF sequence :
MGKSEYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRDAKKTYKAPKEFNEFLAFLHLFLPYRAMEGVLRALEGLKIVPTSLDYSTMWERVRNMD
ITFPEANDQLEVIADATGISTTRGGQYIIAKWGKRRDSKFLKIEIVMDKDELNVIDAEVTSDEVETAVKTKDLQDEGKKVKRFYGDKAYDANEVYNTGVE
VVPPGEDASTGRGHPARRKAVREFKELGYDRWREERGYGVRWRVESLFSAVKRTFGESVGATSFLGQVVEAKLKFWAYAWMVHLANSLVGRAPGIKV
ITFPEANDQLEVIADATGISTTRGGQYIIAKWGKRRDSKFLKIEIVMDKDELNVIDAEVTSDEVETAVKTKDLQDEGKKVKRFYGDKAYDANEVYNTGVE
VVPPGEDASTGRGHPARRKAVREFKELGYDRWREERGYGVRWRVESLFSAVKRTFGESVGATSFLGQVVEAKLKFWAYAWMVHLANSLVGRAPGIKV
Blast result :
Comments
ISMye2 is 95% aa similar to ISSis3.
References
1] ISfinder annotation (2016)
2] Lucas,S., Han,J., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Kozubal,M., Macur,R.E., Jay,Z., Inskeep,W. and Woyke,T. (2012) Direct submission GenBank.
2] Lucas,S., Han,J., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Kozubal,M., Macur,R.E., Jay,Z., Inskeep,W. and Woyke,T. (2012) Direct submission GenBank.