ISAfer1
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LSUQ01000015 | ND | Acidibacillus ferrooxidans | Acidibacillus ferrooxidans SLC66 |
DNA section
IS Length : 1623 bp
Ends
IR Length : 16/20
IRL : GTTACCCCTGAAAAAGTCGGGCATTTTTTGACAAAACAAAAAGGAATTCG
IRR : GTTGCTCCTGAAAAAGTATGAATTTCAAAAATGCATCTTCCTGCCGCACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCGATCTAC | TAGATA | GTTTCACCAT | 6 |
DNA sequence
GTTACCCCTGAAAAAGTCGGGCATTTTTTGACAAAACAAAAAGGAATTCGGCACCTCAAAATGGAAATTGTTATTGTCGAGACAACAAAGTTCCAAACCC
CGAGGGAAGAATTCCTTTTGTTAGGAATTCAACGCAAAGCCTCGGGAATCCTTCCTTGAGGTGAAGAAAATGTTGAAACTTCGCGACGTACAACTTACGA
TGTGGGACGCTATTCTGCTGGAAGCTGTACGTGCACTTCCTGCCGAACTTGCGATCATCGACGCGCTTTTGGATGACGAACGTTTTCTTCAGCCGTTCGT
TAAGCTACACCCGAAGGGTAAGAAAAGCGGCCGTCCCACGTTCGCTATTGAAAAGTATGTACGTCTCATGGTGCTTAAGCACAAATACAATTTGGGTTAC
GAATCGCTGGTAAAGGAAGTTGGCGACAGCATCACCTGGCGGATGTTTTGCCGGATCGCAATCGATGAACTCATGCCGGATCCATCCACGCTCATCTATG
CTCGCAAACGCTACGGCAACAAGATCGTCGAAGATGTCAACGCCGCATTGGTAAAGCAACTCGAGGAAAAAGCGATTCTGAAAACCCGTAAATTCCGGAC
CGACACAACGGTAGTGGAATCCGACATCCATCACCCGACAGACGCCACGCTGCTACAAGATGGTGTAAAAGTCATGAGCCGAATCATTCGAAAAATCCGT
AAGGTCGCTTCTCATGCCGTTCAAGGAATGGAGGATCGCACCTTGGAAATCAAAGAAAAGATTCTGTCCATTGCCAAGGTCCTTCGTCGGCGCACTCGCG
AATCATGGGAAGAAGTCGACAAGATTACGCAAAGCGTGATCGAAATAACCGAAAACGTCGTTTCTCAAGCCCATGCCGTCATCGAGAAATTACAAAACCA
AAGGAAGACCATTGTTCAGCAAAAGAAAGAACGTCTCGTTACCGCGGTTCAGCTTACGGAGAAGCTGCTGGATCAAGCGAGGCAGGTCGTTTCCGGTAAC
CGAATCATTGCAGATCGCATGGTTAGTTTCTTTGACCCGGAAGCAAGACCGATCAAAAAAGGGAAGCTCGCGAAAGGTACCGAGTTCGGGTACAAAGTTC
GGATCGACGAGACAGAAAGCGGCTTCGTTACCGGCTATGCCGTGTATGAAGGAAATCCGTCGGATGATGAATTGCTCGTTCCTGCCATTGAAGAGCACAT
CAAGCAGTTCGGAAAAGCACCGCAGGCCGTAGCCACTGACCGCGGTTTTTCGAGTAAGAAAAACGAAATCGCGATGACTGAACTTGGTGTTAAACGGGCC
AGCCTACCAAAAAAAGGTAAGAAAGGCGCTGCCCGAACGGAATATGAAAATGAAAAATGGTTCAAGGACTTGCAGCGTTACCGTGCAGCTGGAGAAGCGA
AGATTAGCCTTCTCAAGCGGAAGTACGGTTTGAACCGCAGCAGGTACCGGGGATTTGCTGGCTCAAAATGCTGGATCGGATTCGGAATTCTGACCCATAA
CTTGCAAAGGGCGGCAAAGATGCTCCAAAACTAAGGTAAATACGGAAAAACGGGGAGAATCCCCCCAAAAATCAGTGCGGCAGGAAGATGCATTTTTGAA
ATTCATACTTTTTCAGGAGCAAC
CGAGGGAAGAATTCCTTTTGTTAGGAATTCAACGCAAAGCCTCGGGAATCCTTCCTTGAGGTGAAGAAAATGTTGAAACTTCGCGACGTACAACTTACGA
TGTGGGACGCTATTCTGCTGGAAGCTGTACGTGCACTTCCTGCCGAACTTGCGATCATCGACGCGCTTTTGGATGACGAACGTTTTCTTCAGCCGTTCGT
TAAGCTACACCCGAAGGGTAAGAAAAGCGGCCGTCCCACGTTCGCTATTGAAAAGTATGTACGTCTCATGGTGCTTAAGCACAAATACAATTTGGGTTAC
GAATCGCTGGTAAAGGAAGTTGGCGACAGCATCACCTGGCGGATGTTTTGCCGGATCGCAATCGATGAACTCATGCCGGATCCATCCACGCTCATCTATG
CTCGCAAACGCTACGGCAACAAGATCGTCGAAGATGTCAACGCCGCATTGGTAAAGCAACTCGAGGAAAAAGCGATTCTGAAAACCCGTAAATTCCGGAC
CGACACAACGGTAGTGGAATCCGACATCCATCACCCGACAGACGCCACGCTGCTACAAGATGGTGTAAAAGTCATGAGCCGAATCATTCGAAAAATCCGT
AAGGTCGCTTCTCATGCCGTTCAAGGAATGGAGGATCGCACCTTGGAAATCAAAGAAAAGATTCTGTCCATTGCCAAGGTCCTTCGTCGGCGCACTCGCG
AATCATGGGAAGAAGTCGACAAGATTACGCAAAGCGTGATCGAAATAACCGAAAACGTCGTTTCTCAAGCCCATGCCGTCATCGAGAAATTACAAAACCA
AAGGAAGACCATTGTTCAGCAAAAGAAAGAACGTCTCGTTACCGCGGTTCAGCTTACGGAGAAGCTGCTGGATCAAGCGAGGCAGGTCGTTTCCGGTAAC
CGAATCATTGCAGATCGCATGGTTAGTTTCTTTGACCCGGAAGCAAGACCGATCAAAAAAGGGAAGCTCGCGAAAGGTACCGAGTTCGGGTACAAAGTTC
GGATCGACGAGACAGAAAGCGGCTTCGTTACCGGCTATGCCGTGTATGAAGGAAATCCGTCGGATGATGAATTGCTCGTTCCTGCCATTGAAGAGCACAT
CAAGCAGTTCGGAAAAGCACCGCAGGCCGTAGCCACTGACCGCGGTTTTTCGAGTAAGAAAAACGAAATCGCGATGACTGAACTTGGTGTTAAACGGGCC
AGCCTACCAAAAAAAGGTAAGAAAGGCGCTGCCCGAACGGAATATGAAAATGAAAAATGGTTCAAGGACTTGCAGCGTTACCGTGCAGCTGGAGAAGCGA
AGATTAGCCTTCTCAAGCGGAAGTACGGTTTGAACCGCAGCAGGTACCGGGGATTTGCTGGCTCAAAATGCTGGATCGGATTCGGAATTCTGACCCATAA
CTTGCAAAGGGCGGCAAAGATGCTCCAAAACTAAGGTAAATACGGAAAAACGGGGAGAATCCCCCCAAAAATCAGTGCGGCAGGAAGATGCATTTTTGAA
ATTCATACTTTTTCAGGAGCAAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1365 bp | 454 aa | 170 | 1534 | + | No |
Chemistry : DDE
ORF sequence :
MLKLRDVQLTMWDAILLEAVRALPAELAIIDALLDDERFLQPFVKLHPKGKKSGRPTFAIEKYVRLMVLKHKYNLGYESLVKEVGDSITWRMFCRIAIDE
LMPDPSTLIYARKRYGNKIVEDVNAALVKQLEEKAILKTRKFRTDTTVVESDIHHPTDATLLQDGVKVMSRIIRKIRKVASHAVQGMEDRTLEIKEKILS
IAKVLRRRTRESWEEVDKITQSVIEITENVVSQAHAVIEKLQNQRKTIVQQKKERLVTAVQLTEKLLDQARQVVSGNRIIADRMVSFFDPEARPIKKGKL
AKGTEFGYKVRIDETESGFVTGYAVYEGNPSDDELLVPAIEEHIKQFGKAPQAVATDRGFSSKKNEIAMTELGVKRASLPKKGKKGAARTEYENEKWFKD
LQRYRAAGEAKISLLKRKYGLNRSRYRGFAGSKCWIGFGILTHNLQRAAKMLQN
LMPDPSTLIYARKRYGNKIVEDVNAALVKQLEEKAILKTRKFRTDTTVVESDIHHPTDATLLQDGVKVMSRIIRKIRKVASHAVQGMEDRTLEIKEKILS
IAKVLRRRTRESWEEVDKITQSVIEITENVVSQAHAVIEKLQNQRKTIVQQKKERLVTAVQLTEKLLDQARQVVSGNRIIADRMVSFFDPEARPIKKGKL
AKGTEFGYKVRIDETESGFVTGYAVYEGNPSDDELLVPAIEEHIKQFGKAPQAVATDRGFSSKKNEIAMTELGVKRASLPKKGKKGAARTEYENEKWFKD
LQRYRAAGEAKISLLKRKYGLNRSRYRGFAGSKCWIGFGILTHNLQRAAKMLQN
Blast result :
Comments
ISAfer1 is 88% aa similar to ISTco5.
References
1] ISfinder annotation (2016)
2] Oliveira,G., Nancucheo,I., Dall'Agnol,H., Johnson,B., Oliveira,R.,Nunes,G.L., Tzotzos,G., Orellana,S.C., Salim,A.C. and Araujo,F.M. (2016) Direct submission.
2] Oliveira,G., Nancucheo,I., Dall'Agnol,H., Johnson,B., Oliveira,R.,Nunes,G.L., Tzotzos,G., Orellana,S.C., Salim,A.C. and Araujo,F.M. (2016) Direct submission.