ISfinder Mirror Site

IS903B

Family IS5
Group IS903

MGE type ISRelated element(s) :
Isoform Synonym(s) IS903.B, ISKpn36

Accession number	Transposition	Origin	Host
X02527	Y	Escherichia coli	Enterobacterias phage HK022 Escherichia coli plasmid pRts1

DNA section

IS Length : 1057 bp

Ends

IR Length : 18

IRL : GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACG
IRR : GGCTTTGTTGAATAAATCAGATTTCGGGTAAGTCTCCCCCGTAGCGGGTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAGATTTTGA	GACACAACG	TGTTCCCCGC	9
NNNNNNNGAT	CGTTGGGAA	CCGNNNNNNN	9

DNA sequence

GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAAT
CACCAACTGGCCCACCTACAATAAAGCCCTCATCAACCGTGGCTCCATAACTTTCTGGCTGGATGATGAAGCTATTCAGGCCTGGTATGAGTCAGCAACA
CCTTCTTCACGAGGCAGACCTCAGCGCTATTCTGACCTTGCCATCACGACTGTGCTGGTCATTAAACGCGTATTCAGGCTGACCCTGCGCGCTGCCCAGG
GCTTTATTGATTCCATTTTTTCTCTGATGAACGTTCCGCTACGCTGCCCGGATTACAGCTGTGTCAGCAGGCGGGCAAAGTCGGTTAATGTCAGTTTCAA
AACGCCCACCCGGGGTGAAATCGCACACCTGGTAATTGATTCCACCGGGCTGAAGGTCTTCGGTGAAGGCGAGTGGAAAGTCAAAAAGCATGGCCAGGAA
CGCCGTCGTATATGGCGAAAGTTGCATCTGGCCGTTGACAGCAACACACATGAAATCATCTGTGCAGACCTGTCGCTGAACAATGTGACGGACTCAGAAG
CCTTCCCGGGTCTTATCCGGCAGACTCACAGAAAAATCAGGGCAGCAGCGGCAGACGGCGCTTACGACACCCGGCTCTGTCACGATGAACTGCGGCGTAA
GAAAATCAGCGCGCTTATCCCTCCCCGAAAAGGTGCAGGTTACTGGCCCGGTGAATATGCAGACCGTAACCGTGCAGTGGCTAATCAGCGAATGACCGGG
AGTAATGCGCGGTGGAAATGGACAACAGATTACAACCGTCGCTCGATAGCGGAAACGGCGATGTACCGGGTAAAACAGCTGTTCGGGGGTTCACTGACGC
TGCGTGACTACGATGGTCAGGTTGCGGAGGCTATGGCCCTGGTACGAGCGCTGAACAAAATGACGAAAGCAGGTATGCCTGAAAGCGTGCGTATTGCCTG
AAAACACAACCCGCTACGGGGGAGACTTACCCGAAATCTGATTTATTCAACAAAGCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
924 bp	307 aa	78	1001	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKQKFKITNWPTYNKALINRGSITFWLDDEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLRCPDYSCVSRRA
KSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRL
CHDELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGM
PESVRIA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
213 bp	70 aa	672	460	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MCVAVNGQMQLSPYTTAFLAMLFDFPLAFTEDLQPGGINYQVCDFTPGGRFETDINRLCPPADTAVIRAA

Blast result :

Comments

IS903B differs from IS903 and IS102 by 34 and 61 nt, respectively.
Additionnal Accession number :
NC_002166 : This sequence is 99% aa similar to the sequence of IS903B ORF1.
KT362307 : this sequence is also 99% aa similar to IS903B.
Found in Tn2680.

References

1] Mollet, B., Iida, S., and Arber, W. (1985) An active variant of the prokaryotic transposable element IS903 carries an amber stop codon in the middle of an open reading frame. Mol. Gen. Genet. 199, 534-536.
2] Mollet, B., Clerget, M., Meyer, J., and Iida, S. (1985). J. Bacteriol. 163, 55-60.
3] Juhala,R., Ford,M.E., Duda,R.L., Youlton,A., Hatfull,G.F. and Hendrix,R.W. (1998) Direct submission GenBank.
4] Li,X., Yu,Y. and Fu,Y. (2015) Direct GenBank submission.