ISfinder Mirror Site

ISSan1

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HM038106	ND	Streptomyces anulatus	Streptomyces anulatus ATCC 11523

DNA section

IS Length : 1071 bp

Ends

IR Length : 21/22

IRL : GGCCTGTGTGATGTCGTGATCACTCGGCGGTCTTCAGGAGGTCGTCGATC
IRR : GGCCTGTGTGATGTTGTGATCAAGTTCTGGGTTCTGCCCTCTTCGCGGGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCCTGGTCGA	CTAG	CCGCTCGACG	4

DNA sequence

GGCCTGTGTGATGTCGTGATCACTCGGCGGTCTTCAGGAGGTCGTCGATCCAGATCATCGAGGCACGAAGGTGGAGGCCGGCGAGGTAGCTTTCGGGTGT
CTTGTCGTAGCGGGTGGCGATGCCTCGCCAGGCTTTCAGCTTGTTGATCAGACGCTCGACGGTGTTGCGTTCCTTGTAGAGGTCGGCGTCGTGGTTGAGG
GGCCGGCCGCCTCGGGCGCCTTTCTTCTTCCGGTTGGCGGCCTGGTCCTTCTTCTCCGGGATGACTGCTTTGATGCCGCGTCTGCGCAGGTAGGAGCGGT
TACCGCGGGATGAGTAGGCCTTGTCCGCGGCGACTGCGCCGGGTCTGGTGCGGGGACGGCCGGCCGGCAGGCGGATTCGCACCTTCGCGAGGACGGGGAT
GAACTGCGGGCTGTCCCCGGCCTGCCCGGCGGTCAGGACCAGCGCGAGGGGGCGGCATCTGCGGTCCGCGGCGAGGTGGATCTTGCTGGTCAGTCCGCCC
CTGGACCTGCCCAGCAGGGCCTCCTTCAGCCGGAGTCGCTGTCGGCGGCGGATGCTTCGACGCTCGACCTGTCCGTATTGCTCCTCCGCCCCACCCCTTT
TTTCCGGGCCTTCTCCTGCTCCTGCGCGGCTTCCTCCAGAGCGTCCATGAGGTGCCTGCTGACGCGCATCCCCGCGGCGTCATGGTGGCCGCGGACCGTG
GTGGAGTCCACGCTGACCAGCGACAGGTCCGTTCTTCCCACACGGGCGCCCTCGGCAATCAGACCTTCCAGCAGGGCGGTGAACACACCTGCGTCCCGCC
AGACTCGGAAGCGTCCGTAGACGGTCGCCCAGGGCCCGAACTCGGCAGGCATCTCGCGCCACTGGCCGCTCGACCGGAACCGCCAGATCACCCCCTCGAA
CTGCTCGCGCAACCGCTCCGGGTACGGACCGGACCGGCCGATCGGCAAGTACGGCTCGATGAACTTCCACTGGGCATGGGTGAGTTGCCTGCGCGTCACA
CCCCAGATCTACCGGATTCCACCCCGCGAAGAGGGCAGAACCCAGAACTTGATCACAACATCACACAGGCC

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
474 bp	157 aa	73	546	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

VTRRQLTHAQWKFIEPYLPIGRSGPYPERLREQFEGVIWRFRSSGQWREMPAEFGPWATVYGRFRVWRDAGVFTALLEGLIAEGARVGRTDLSLVSVDST
TVRGHHDAAGMRVSRHLMDALEEAAQEQEKARKKGVGRRSNTDRSSVEASAADSDSG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
660 bp	219 aa	393	1052	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RRGDARQQAPHGRSGGSRAGAGEGPEKRGGAEEQYGQVERRSIRRRQRLRLKEALLGRSRGGLTSKIHLAADRRCRPLALVLTAGQAGDSPQFIPVLAKV
RIRLPAGRPRTRPGAVAADKAYSSRGNRSYLRRRGIKAVIPEKKDQAANRKKKGARGGRPLNHDADLYKERNTVERLINKLKAWRGIATRYDKTPESYLA
GLHLRASMIWIDDLLKTAE

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
980 bp	326 aa	73	1052	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VTRRQLTHAQWKFIEPYLPIGRSGPYPERLREQFEGVIWRFRSSGQWREMPAEFGPWATVYGRFRVWRDAGVFTALLEGLIAEGARVGRTDLSLVSVDST
TVRGHHDAAGMRVSRHLMDALEEAAQEQEKARKKRGGAEEQYGQVERRSIRRRQRLRLKEALLGRSRGGLTSKIHLAADRRCRPLALVLTAGQAGDSPQF
IPVLAKVRIRLPAGRPRTRPGAVAADKAYSSRGNRSYLRRRGIKAVIPEKKDQAANRKKKGARGGRPLNHDADLYKERNTVERLINKLKAWRGIATRYDK
TPESYLAGLHLRASMIWIDDLLKTAE

Blast result :

Comments

ISSan1 is 51% aa similar to IS1650.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Based on comparison with other IS427 subgroup members, the transposase of ISSan1 is 50 aa longer(maybe an insertion in the middle of the transposase).

References

1] Keller U. (2015) Direct submission
2] Pfennig,F., Schauwecker,F. and Keller,U. (1999) J. Biol. Chem. 274 (18), 12508-12516
3] Schauwecker,F., Pfennig,F., Grammel,N. and Keller,U. (2000) Chem. Biol. 7 (4), 287-297
4] Keller,U., Lang,M., Crnovcic,I., Pfennig,F. and Schauwecker,F. (2010) J. Bacteriol. 192 (10), 2583-2595