ISEnu1
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JOKH01000003 | ND | Endozoicomonas numazuensis | Endozoicomonas numazuensis DSM 25634 |
DNA section
IS Length : 1818 bp
Ends
IR Length : 13/15
IRL : GTGCCTGAACGAAAATCCTCGAATAGCTTATAGATCAAGACGTCTACGTC
IRR : GTGCCCGAACGGAAAATGCTCTTTTCTGTTCGAAGTTCAGCCTTCATAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAGTTCTCT | TATATA | GAAACGTTTG | 6 |
DNA sequence
GTGCCTGAACGAAAATCCTCGAATAGCTTATAGATCAAGACGTCTACGTCATATTTACCGTATGAAGTTTTTCCTAAAGCTTGGCTTTTAACGCTAGAAT
AGCCCTAATCATGTCTATTTCCTTCATTCTTCGGCTCAAAAATAATCATGCGACAGGTACAATCCAGACAACTTCAGATCGGTGAAGTGGATATTGGTAG
CATTGAACTCGATCATCGGTCCCGTGATGATATTCCCCAGATCCTGCTTGGCTTACAACACCTCTATTTGAATGCCGAAGCCCGGGATTTAGTCTTTTCT
CTTCTGGAGCAGGCTGTCCCTGAAAATATCAGCCCTGACAGGGGGCGACCAGGAATGCAGCTGTGGAAGATCCTTGTTCTGGGTGTACTCCGGTTAAATC
TTGATTGGAACTATGACCGGCTTCGGGAAATGGCCAACCAACACGCCACCATCCGGGCAATGCTCGGTCACGGGATGTATGACCTGTATGAATATAAGCT
CCAGACGATTAAGGATAACGTCTCACTTTTAACCCCCGAACTGCTCAATCAGATTAATGAAGTCGTTGTGGCTGAAGGCCATAAAATAGTTGGAAAAAAA
AAGAAGATCCGCTGAGAGGTCGCTGTGACTCTTATGTTTTAGAAACCGATGTTCATTTCCCGACAGATCTCAACCTTTTGTTTGATGCAGTCAGAACTGT
CGTTGTTCTTACTGCACGCTTTGCAGCCAGGGCTGAGGTAAGTGGTTGGCGACAGTGGCAATACAACCTGAATCAGTTGAAAAAAATGTATCGTCGTGTA
CAGATATTACGACACTCTACTTCGAAAGATCCTGCCAAAAAACTTCAAAGGGACAAGGAAATCCGACAAGCCTGTCAGAACTACCTGAATGGCAGTCAGG
CATTGATTAACAGAGCTGAAGAAACACTTCAGGACAAGGCGTTCATTACGATATGTCCAGCGAATAAATACAGTTCTATCCAGAAGTTTATCGATCATGG
TAAGCGGCAGGTGGATCATGTTAAACGTCGATTGATCGACCATGAAAAAATCCCTCATGATGAAAAAGTATTCTCGCTCTTTCAGCCGCATACTGAGTGG
ATCAATAAAGGTAAAGCAGGTGTTCCGGTTGAGCTTGGCGTCCGTTTGGCGATTGTCGAAGACCAGTATGGTTTTATTCTGAACCATCGGGTCATGGAAA
AAGAAACCGATGAAAAGGCTGCCGTTCCGATCATCCGGGCAACCAAAGCCGCCTTTCCATCTTTGAGCGTTTGCAGTTTTGATAAAGGGTTTCACTCACC
ATCCAACCAAAAAGATTTGCTTAAATACCTTACTATGGTCGTATTACCAAAAAAGGGACGACGAACCCGGGCCGAGCAGGAGCGGGAAAACGACCCGGAA
TTCAAAGCGGCTAAAAGAAAGCACTCGGCGGTAGAATCAGCGATCAATGCACTTGAGTCTCACGGGTTGGATAAATGCGCGGATCACGGAATAGATGGAC
TGAAACGCTATGTTGCTCTCGGAGTTCTTGCCAGGAATTTTCAGAAGATGGGGGCAGAGCTTCAGAAAATAGAACGAAAGAGCGCAAAAAAACGACAACT
TCGACTCTGTGGCTGAAAGCACTGACGGTTAATTCGAAAAAAGAATAAAATCAGTAAAGACTGAGGTCGTCGCTTGTCTTGAAATTGCAGAAATGACGAG
TTTGGTAAGAAAATTACGATGAAACTGGCAAATCAGAAAGTCTATTGATCGATTATTCAAATATCTGGCTTATGAAGGCTGAACTTCGAACAGAAAAGAG
CATTTTCCGTTCGGGCAC
AGCCCTAATCATGTCTATTTCCTTCATTCTTCGGCTCAAAAATAATCATGCGACAGGTACAATCCAGACAACTTCAGATCGGTGAAGTGGATATTGGTAG
CATTGAACTCGATCATCGGTCCCGTGATGATATTCCCCAGATCCTGCTTGGCTTACAACACCTCTATTTGAATGCCGAAGCCCGGGATTTAGTCTTTTCT
CTTCTGGAGCAGGCTGTCCCTGAAAATATCAGCCCTGACAGGGGGCGACCAGGAATGCAGCTGTGGAAGATCCTTGTTCTGGGTGTACTCCGGTTAAATC
TTGATTGGAACTATGACCGGCTTCGGGAAATGGCCAACCAACACGCCACCATCCGGGCAATGCTCGGTCACGGGATGTATGACCTGTATGAATATAAGCT
CCAGACGATTAAGGATAACGTCTCACTTTTAACCCCCGAACTGCTCAATCAGATTAATGAAGTCGTTGTGGCTGAAGGCCATAAAATAGTTGGAAAAAAA
AAGAAGATCCGCTGAGAGGTCGCTGTGACTCTTATGTTTTAGAAACCGATGTTCATTTCCCGACAGATCTCAACCTTTTGTTTGATGCAGTCAGAACTGT
CGTTGTTCTTACTGCACGCTTTGCAGCCAGGGCTGAGGTAAGTGGTTGGCGACAGTGGCAATACAACCTGAATCAGTTGAAAAAAATGTATCGTCGTGTA
CAGATATTACGACACTCTACTTCGAAAGATCCTGCCAAAAAACTTCAAAGGGACAAGGAAATCCGACAAGCCTGTCAGAACTACCTGAATGGCAGTCAGG
CATTGATTAACAGAGCTGAAGAAACACTTCAGGACAAGGCGTTCATTACGATATGTCCAGCGAATAAATACAGTTCTATCCAGAAGTTTATCGATCATGG
TAAGCGGCAGGTGGATCATGTTAAACGTCGATTGATCGACCATGAAAAAATCCCTCATGATGAAAAAGTATTCTCGCTCTTTCAGCCGCATACTGAGTGG
ATCAATAAAGGTAAAGCAGGTGTTCCGGTTGAGCTTGGCGTCCGTTTGGCGATTGTCGAAGACCAGTATGGTTTTATTCTGAACCATCGGGTCATGGAAA
AAGAAACCGATGAAAAGGCTGCCGTTCCGATCATCCGGGCAACCAAAGCCGCCTTTCCATCTTTGAGCGTTTGCAGTTTTGATAAAGGGTTTCACTCACC
ATCCAACCAAAAAGATTTGCTTAAATACCTTACTATGGTCGTATTACCAAAAAAGGGACGACGAACCCGGGCCGAGCAGGAGCGGGAAAACGACCCGGAA
TTCAAAGCGGCTAAAAGAAAGCACTCGGCGGTAGAATCAGCGATCAATGCACTTGAGTCTCACGGGTTGGATAAATGCGCGGATCACGGAATAGATGGAC
TGAAACGCTATGTTGCTCTCGGAGTTCTTGCCAGGAATTTTCAGAAGATGGGGGCAGAGCTTCAGAAAATAGAACGAAAGAGCGCAAAAAAACGACAACT
TCGACTCTGTGGCTGAAAGCACTGACGGTTAATTCGAAAAAAGAATAAAATCAGTAAAGACTGAGGTCGTCGCTTGTCTTGAAATTGCAGAAATGACGAG
TTTGGTAAGAAAATTACGATGAAACTGGCAAATCAGAAAGTCTATTGATCGATTATTCAAATATCTGGCTTATGAAGGCTGAACTTCGAACAGAAAAGAG
CATTTTCCGTTCGGGCAC
Recoding section
- Recoding by frameshift
- Frame -1
- Type transcriptional
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence : No
- Secondary structure : No-structure
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 468 bp | 155 aa | 148 | 615 | + | No |
Description : First part of the transposase
ORF sequence :
MRQVQSRQLQIGEVDIGSIELDHRSRDDIPQILLGLQHLYLNAEARDLVFSLLEQAVPENISPDRGRPGMQLWKILVLGVLRLNLDWNYDRLREMANQHA
TIRAMLGHGMYDLYEYKLQTIKDNVSLLTPELLNQINEVVVAEGHKIVGKKKKIR
TIRAMLGHGMYDLYEYKLQTIKDNVSLLTPELLNQINEVVVAEGHKIVGKKKKIR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 585 | 1616 | + | No |
Description : Second part of the transposase
ORF sequence :
NSWKKKEDPLRGRCDSYVLETDVHFPTDLNLLFDAVRTVVVLTARFAARAEVSGWRQWQYNLNQLKKMYRRVQILRHSTSKDPAKKLQRDKEIRQACQNY
LNGSQALINRAEETLQDKAFITICPANKYSSIQKFIDHGKRQVDHVKRRLIDHEKIPHDEKVFSLFQPHTEWINKGKAGVPVELGVRLAIVEDQYGFILN
HRVMEKETDEKAAVPIIRATKAAFPSLSVCSFDKGFHSPSNQKDLLKYLTMVVLPKKGRRTRAEQERENDPEFKAAKRKHSAVESAINALESHGLDKCAD
HGIDGLKRYVALGVLARNFQKMGAELQKIERKSAKKRQLRLCG
LNGSQALINRAEETLQDKAFITICPANKYSSIQKFIDHGKRQVDHVKRRLIDHEKIPHDEKVFSLFQPHTEWINKGKAGVPVELGVRLAIVEDQYGFILN
HRVMEKETDEKAAVPIIRATKAAFPSLSVCSFDKGFHSPSNQKDLLKYLTMVVLPKKGRRTRAEQERENDPEFKAAKRKHSAVESAINALESHGLDKCAD
HGIDGLKRYVALGVLARNFQKMGAELQKIERKSAKKRQLRLCG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1469 bp | 490 aa | 148 | 1616 | + | Yes |
Chemistry : DDE
ORF sequence :
MRQVQSRQLQIGEVDIGSIELDHRSRDDIPQILLGLQHLYLNAEARDLVFSLLEQAVPENISPDRGRPGMQLWKILVLGVLRLNLDWNYDRLREMANQHA
TIRAMLGHGMYDLYEYKLQTIKDNVSLLTPELLNQINEVVVAEGHKIVGKKKKEDPLRGRCDSYVLETDVHFPTDLNLLFDAVRTVVVLTARFAARAEVS
GWRQWQYNLNQLKKMYRRVQILRHSTSKDPAKKLQRDKEIRQACQNYLNGSQALINRAEETLQDKAFITICPANKYSSIQKFIDHGKRQVDHVKRRLIDH
EKIPHDEKVFSLFQPHTEWINKGKAGVPVELGVRLAIVEDQYGFILNHRVMEKETDEKAAVPIIRATKAAFPSLSVCSFDKGFHSPSNQKDLLKYLTMVV
LPKKGRRTRAEQERENDPEFKAAKRKHSAVESAINALESHGLDKCADHGIDGLKRYVALGVLARNFQKMGAELQKIERKSAKKRQLRLCG
TIRAMLGHGMYDLYEYKLQTIKDNVSLLTPELLNQINEVVVAEGHKIVGKKKKEDPLRGRCDSYVLETDVHFPTDLNLLFDAVRTVVVLTARFAARAEVS
GWRQWQYNLNQLKKMYRRVQILRHSTSKDPAKKLQRDKEIRQACQNYLNGSQALINRAEETLQDKAFITICPANKYSSIQKFIDHGKRQVDHVKRRLIDH
EKIPHDEKVFSLFQPHTEWINKGKAGVPVELGVRLAIVEDQYGFILNHRVMEKETDEKAAVPIIRATKAAFPSLSVCSFDKGFHSPSNQKDLLKYLTMVV
LPKKGRRTRAEQERENDPEFKAAKRKHSAVESAINALESHGLDKCADHGIDGLKRYVALGVLARNFQKMGAELQKIERKSAKKRQLRLCG
Blast result :Comments : This ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Comments
ISEnu1 is 72% aa similar to ISMags1.
References
1] ISfinder annotation (2016)
2] Neave,M.J., Michell,C.T., Apprill,A. and Voolstra,C.R. (2014) Genome Announc 2 (4), e00802-14.
2] Neave,M.J., Michell,C.T., Apprill,A. and Voolstra,C.R. (2014) Genome Announc 2 (4), e00802-14.