ISfinder Mirror Site

ISSymo1

Family ISNCY
Group ISDol1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP004885	ND	Candidatus Symbiobacter	Candidatus Symbiobacter mobilis CR

DNA section

IS Length : 1906 bp

Ends

IR Length : 15/19

IRL : GTATCTGACAGAAAAATAGAAAACCATGCAAAATCAACAGGGTACGCTAC
IRR : GTGGCTGACAGAAAAGTCGGATTTTTGATGCGTGTACCCGGTACTTTCAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCTACTTTAC	TACCTG	CAGATGATTT	6
GTTTTATGAC	GAGCTA	GGAATGTGGC	6
CACCACCGCG	TAGGTA	AGCAAGCTGT	6
CCTCGGCTTA	TATCTA	GGCATTTATC	6
AAGGGTGTGG	TAAGTA	AGGATCACGT	6
CATCTACTGT	GACATA	GGGGGATTCT	6

DNA sequence

GTATCTGACAGAAAAATAGAAAACCATGCAAAATCAACAGGGTACGCTACGTTTGGGTGGGTGAAGATTGAGCCGCTTTCCTGCGATTTTCATGAGATTA
TGCATAAGATGTCGCATTACACACAAAATTTTTAATTTTGATATAATATTTTCATCCTACTTCAATCTTCATTAAAATAATTGGGAACTACATGCGCTCT
GTCATAAATCCTCAGATGAAATTAGGCGAAGATCCTATCGCCAACATTCAACTTGATCTGCGTTCTCGCGATGATATCCCAAAATTGCTGCTAGGACTTC
AGTACATCTACACAATACCAGACGTACGCAAAGAAGTTTTCTCTATCCTCTCAGAAATTCTCCCGGTACTCCCTTCTGGTGAAAAAGTAAAAGCACATAT
GGGTCGCCCAGGTATGTCGCAGTGGCAAATTCTTGTCATCGGTGTGGTTCGATTGGGTCTGAATATCGATTACGACCGATTGGAAGAGCTGGTGAATCAA
CATCGGACATTGCGACAAATGCTCGGGCATACTGACTGGTATGATGAGACAAAATACAACGTGCAAACCCTGAAAGATAATTTGAGGTTATTTACCCCAG
AGATTCTTGATCGAATCAATCAAGTTGTGGTCAAAACCGGCCATACGCTAGTAAAAAAAAGCCCGGACGACGTTCTACACGGTCGTTGTGATTCCTTTGT
CGTTGAAACAGATGTACATTTTCCAACCGATTTGAATCTTCTTTTTGATGCTATAAAAAAGATCATTGCATGGAGTGCAAAGTTAGCGGAGAAACATAAA
TTGTCTGGCTGGCGTCAGCATGAACACTCTTATCGCCAATTCAAAAAGCAGTATCGCCATATCCAGCGGCTTCGGCGCTCTCATTCAAAAAATGAAGCCA
AACGTGCAGCAAAGGAAGCTGCCATCCACGAAGCATGCGAGGAATATCTCAAGCAAGCCAAAGAGCTGTTCGAGCGCGCCACCCAAACAAGGCAGGAAGT
CGTCTTGGCTGCATCGCAGATCAGCGCACAGGAGCAAGGGTTGCTATCGAAGCTGCAGGTCTTCATAGGGCATGCAGAAAAGTTATCAGATCAGATTCGC
CGTAGGGTGATCAACGGGGAACGCATTCCGCATTCGGAAAAGATATTCTCGCTGTTTGAACCGCATACGGAGTGGATAAGCAAAGGCAAGGCTGGTGTGC
CTGTGGAATTGGGGGTAAAGGTTTGTATTCTGGAAGATCAACATCGTTTCATTCTGCATCATCACGTTATGGAGAAGCAAGAAGATAACGAAGTTGCTGT
ACCAATGGTTGAAGCTGCCAAAGAACGATTCTCGACTTTGAATGCCGTGAGTTTTGACAAAGGATTCCACAGTCAAGAGAATCAGAAAAAATTGGCAAAA
TTATTAGACTGTGCTGCTTTACCAAAGAAAGGGCGCTGTTCTCAAGCGGAACAAGAACGAGAATCGAGCGAGACATTTGTGAAACTACGGAAAGCACATT
CTGCTGTAGAGTCGGCGATCAATGGATTGGAACACTTTGGTTTGGATATGTGCCCCGATGAGGGAATAAAAGGATTCAAACGCTATGTTTCATTGTCAGT
CTTGGCACGAAATTTTTACCGTATTGGCATGGTGGTTCGTCAGCAATCGATTGATGCCGAGCGAACGACACCACCATCCATTCGTCGAGCGGCTTGATGC
CGCTCGACGGTCTTTTTCAAGGGATGCATGAGTGGTTGCAGAGGATAGCTTTTCCTTCATTCACCAATAATTCAATTTTTGGAGCAGGATACATGGTTTT
TGTCAAAATTCGACCGCAAAAAAATTTGTAATCCGGTCTAGGTTCCGGATCACTTCCTGAAAGTACCGGGTACACGCATCAAAAATCCGACTTTTCTGTC
AGCCAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1506 bp	501 aa	192	1697	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRSVINPQMKLGEDPIANIQLDLRSRDDIPKLLLGLQYIYTIPDVRKEVFSILSEILPVLPSGEKVKAHMGRPGMSQWQILVIGVVRLGLNIDYDRLEEL
VNQHRTLRQMLGHTDWYDETKYNVQTLKDNLRLFTPEILDRINQVVVKTGHTLVKKSPDDVLHGRCDSFVVETDVHFPTDLNLLFDAIKKIIAWSAKLAE
KHKLSGWRQHEHSYRQFKKQYRHIQRLRRSHSKNEAKRAAKEAAIHEACEEYLKQAKELFERATQTRQEVVLAASQISAQEQGLLSKLQVFIGHAEKLSD
QIRRRVINGERIPHSEKIFSLFEPHTEWISKGKAGVPVELGVKVCILEDQHRFILHHHVMEKQEDNEVAVPMVEAAKERFSTLNAVSFDKGFHSQENQKK
LAKLLDCAALPKKGRCSQAEQERESSETFVKLRKAHSAVESAINGLEHFGLDMCPDEGIKGFKRYVSLSVLARNFYRIGMVVRQQSIDAERTTPPSIRRA
A

Blast result :

Comments

ISSymo1 is 70% aa similar to ISPph5.

References

1] ISfinder annotation (2016)
2] Liu,Z., Muller,J., Li,T., Alvey,R.M., Vogl,K., Frigaard,N.U., Rockwell,N.C., Boyd,E.S., Tomsho,L.P., Schuster,S.C., Henke,P., Rohde,M., Overmann,J. and Bryant,D.A. (2013) Genome Biol. 14 (11), R127.