ISLad1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Leclercia adecarboxylata | Leclercia adecarboxylata |
DNA section
IS Length : 1249 bp
Ends
IR Length : 21/27
IRL : TGAAGTGGTTTACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACC
IRR : TGAAGTGGTCAACAAAAACTGGCCACCGAGTTAGAGTTTTTCCAGTATCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACCATCGAG | GAG | GGGCGTAAGG | 3 |
DNA sequence
TGAAGTGGTTTACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCACCAATGAAAAGAAGACATTTTAGTCCTGAAT
TCAAACGCGAATCCGCTCAGCTGGTTGTCGACCAAAATTACACTGTCTCTGACGCTGCTAAGGCAATGGATGTTGGTCTTTCCACGATGACGAAATGGGT
CAAACAACTGCGTGATGAGCGTCAGGGTAAAACGCCAAAAGCTTCCCCGATTACGCCGGAACAAATCGAAATACGTGAGCTAAAGAAAAAGCTGCAACGC
ATTGAAATGGAAAACGATATTCTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGTTAATCGGGAAACTCAGGGCGCGTTATCC
TGTGGCCACACTGTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGAGCTGCATTACGC
AGCCAGGTACTTGCACTGCATGGCATCAGTCGTGGTTCGGCCGGGGCAAGGAGCATAGCCACTATGGCAACCCAGAGAGGATATCAGATGGGGCGCTGGC
TTGCTGGCAGGCTAATGAAAGAGCTGGGGTTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCATGTTGCCATCCCGAATCA
CCTTGAGCGACAGTTCGCTGTAACAGAACCAAACCAGGTGTGGTGCGGTGACGTGACTTATATCTGGACGGGTAAGCGCTGGGCGTACCTCGCTGTTGTT
CTCGATCTGTTCGCAAGAAAACCAGTGGGCTGGGCAATGTCGTTCTCGCCGGACAGCAGACTCACCATGAAAGCGCTGGAAATGGCATGGGAAACGCGCG
GTAAGCCCGTAGCGGTGATGTTCCACAGTGATCAGGGCAGCCATTATACGAGCAGGCAGTTCCGGCAGTTACTGTGGCGATACCGGATTAAGCAGAGTAT
GAGTCGGCGCGGAAACTGCTGGGATAATAGCCCAATGGAGCGCTTCTTCAGGAGTCTGAAGAACGAATGGGTGCCGGTGACGGGTTACGTAAGCTTCAGC
GATGCAGCTCGCGCAATAACGGACTATATCGTTGGATATTACAGCACACTAAGACCTCACGAATATAATGGTGGGTTACCACCAAACGAATCGGAAAATC
GATACTGGAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGACCACTTCA
TCAAACGCGAATCCGCTCAGCTGGTTGTCGACCAAAATTACACTGTCTCTGACGCTGCTAAGGCAATGGATGTTGGTCTTTCCACGATGACGAAATGGGT
CAAACAACTGCGTGATGAGCGTCAGGGTAAAACGCCAAAAGCTTCCCCGATTACGCCGGAACAAATCGAAATACGTGAGCTAAAGAAAAAGCTGCAACGC
ATTGAAATGGAAAACGATATTCTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGTTAATCGGGAAACTCAGGGCGCGTTATCC
TGTGGCCACACTGTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGAGCTGCATTACGC
AGCCAGGTACTTGCACTGCATGGCATCAGTCGTGGTTCGGCCGGGGCAAGGAGCATAGCCACTATGGCAACCCAGAGAGGATATCAGATGGGGCGCTGGC
TTGCTGGCAGGCTAATGAAAGAGCTGGGGTTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCATGTTGCCATCCCGAATCA
CCTTGAGCGACAGTTCGCTGTAACAGAACCAAACCAGGTGTGGTGCGGTGACGTGACTTATATCTGGACGGGTAAGCGCTGGGCGTACCTCGCTGTTGTT
CTCGATCTGTTCGCAAGAAAACCAGTGGGCTGGGCAATGTCGTTCTCGCCGGACAGCAGACTCACCATGAAAGCGCTGGAAATGGCATGGGAAACGCGCG
GTAAGCCCGTAGCGGTGATGTTCCACAGTGATCAGGGCAGCCATTATACGAGCAGGCAGTTCCGGCAGTTACTGTGGCGATACCGGATTAAGCAGAGTAT
GAGTCGGCGCGGAAACTGCTGGGATAATAGCCCAATGGAGCGCTTCTTCAGGAGTCTGAAGAACGAATGGGTGCCGGTGACGGGTTACGTAAGCTTCAGC
GATGCAGCTCGCGCAATAACGGACTATATCGTTGGATATTACAGCACACTAAGACCTCACGAATATAATGGTGGGTTACCACCAAACGAATCGGAAAATC
GATACTGGAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGACCACTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 73 | 375 | + | No |
Description : First part of the transposase
ORF sequence :
MKRRHFSPEFKRESAQLVVDQNYTVSDAAKAMDVGLSTMTKWVKQLRDERQGKTPKASPITPEQIEIRELKKKLQRIEMENDILKKATALLMSDSLNSSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 306 | 1241 | + | No |
Description : Second part of the transposase
ORF sequence :
NGKRYSKKGYRALDVRLPEQFSLIGKLRARYPVATLCHVFGVHRSSYKYWKNRPEKPDGRRAALRSQVLALHGISRGSAGARSIATMATQRGYQMGRWLA
GRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGK
PVAVMFHSDQGSHYTSRQFRQLLWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSDAARAITDYIVGYYSTLRPHEYNGGLPPNESENRY
WKNSNSVASFC
GRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGK
PVAVMFHSDQGSHYTSRQFRQLLWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSDAARAITDYIVGYYSTLRPHEYNGGLPPNESENRY
WKNSNSVASFC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 389 aa | 73 | 1241 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRRHFSPEFKRESAQLVVDQNYTVSDAAKAMDVGLSTMTKWVKQLRDERQGKTPKASPITPEQIEIRELKKKLQRIEMENDILKKGYRALDVRLPEQFS
LIGKLRARYPVATLCHVFGVHRSSYKYWKNRPEKPDGRRAALRSQVLALHGISRGSAGARSIATMATQRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRG
GHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPVAVMFHSDQGSHYTSRQFRQL
LWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSDAARAITDYIVGYYSTLRPHEYNGGLPPNESENRYWKNSNSVASFC
LIGKLRARYPVATLCHVFGVHRSSYKYWKNRPEKPDGRRAALRSQVLALHGISRGSAGARSIATMATQRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRG
GHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPVAVMFHSDQGSHYTSRQFRQL
LWRYRIKQSMSRRGNCWDNSPMERFFRSLKNEWVPVTGYVSFSDAARAITDYIVGYYSTLRPHEYNGGLPPNESENRYWKNSNSVASFC
Blast result :
Comments
ISLad1 is 98% aa similar to ISEc52.
References
[1] Zhou D. (2016) Direct submission