ISAba37
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KU744946 | ND | Acinetobacter baumannii | Acinetobacter baumannii strain A297 (RUH875) |
DNA section
IS Length : 872 bp
Ends
IR Length : 18/22
IRL : GAGCCTATCCAGTATTAATTTCATTCTTTTTTAGCACTTAAAAAAAGAAT
IRR : GAACCTATCCAGAATATTTTCTATGCAAACGTAGCCTTAAAAAGGCTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGGTTGGTC | TTA | ATTCACACGA | 3 |
DNA sequence
GAGCCTATCCAGTATTAATTTCATTCTTTTTTAGCACTTAAAAAAAGAATTTGTTAAAATTATTCAATGAGAAAACCATATCCCAGCGACATTAGTCGAG
AAGCCTTTAAAGAAATTGAACCGTTACTATTGAGCGGAAGAAAACAAACACGTCCAAGAGTCGTTGATGTCTATGAAATATTTTGCGCCTTACTCTATAT
TTTAAAAAGTGGATGCCAATGGAGTATGCTTCCTCGAGACTTCCCAGCTAAAAGCACTGTGCATTATTACTTCTCGATTTGGAATAAAAAACCATCAGAA
ACTGAACCTAGCATACTTGAGCAAGCTTTAAAAAAATGTGGTTGGCGAGGCCCGTATCAGCAATGGACGGAAAGAACAAACGAGCTTTATCATCATTGAT
TCACAAAGTGTAAAAAACACAGATACTGCACGTGATAAAGGCTATGACGCTGGTAAAAAAGTTTCAGGCATTAAAAGACATATTATTGTTGATACTCAAG
GTCTCCCGCATGGAATATTGGTTACGACAGCAGATAAAACGGATCGCCAAGGTGCATTAGATGCCATAACTATACATAGAGATACCTTACAAAATGTAGA
GGTTGTACTTGTTGATGGTGGTTATACAGGCGAACCCTTTTATTTGGCTGTTAAGAATTTAATCAGAGCCCGAGTTGAAGTTGCTAAACGAAGTGAATTA
CATAAATTTGCAGTCATTCCACAACGCTGGGTTGTGGAAAGATCATTTGCTTGGTTAGAAAAATGCAGAAGGCTCTGGAAAAACTGTGAAAAGCACCTAA
ATACGAGCCTTCAGTTCATTAATTTAGCCTTTTTAAGGCTACGTTTGCATAGAAAATATTCTGGATAGGTTC
AAGCCTTTAAAGAAATTGAACCGTTACTATTGAGCGGAAGAAAACAAACACGTCCAAGAGTCGTTGATGTCTATGAAATATTTTGCGCCTTACTCTATAT
TTTAAAAAGTGGATGCCAATGGAGTATGCTTCCTCGAGACTTCCCAGCTAAAAGCACTGTGCATTATTACTTCTCGATTTGGAATAAAAAACCATCAGAA
ACTGAACCTAGCATACTTGAGCAAGCTTTAAAAAAATGTGGTTGGCGAGGCCCGTATCAGCAATGGACGGAAAGAACAAACGAGCTTTATCATCATTGAT
TCACAAAGTGTAAAAAACACAGATACTGCACGTGATAAAGGCTATGACGCTGGTAAAAAAGTTTCAGGCATTAAAAGACATATTATTGTTGATACTCAAG
GTCTCCCGCATGGAATATTGGTTACGACAGCAGATAAAACGGATCGCCAAGGTGCATTAGATGCCATAACTATACATAGAGATACCTTACAAAATGTAGA
GGTTGTACTTGTTGATGGTGGTTATACAGGCGAACCCTTTTATTTGGCTGTTAAGAATTTAATCAGAGCCCGAGTTGAAGTTGCTAAACGAAGTGAATTA
CATAAATTTGCAGTCATTCCACAACGCTGGGTTGTGGAAAGATCATTTGCTTGGTTAGAAAAATGCAGAAGGCTCTGGAAAAACTGTGAAAAGCACCTAA
ATACGAGCCTTCAGTTCATTAATTTAGCCTTTTTAAGGCTACGTTTGCATAGAAAATATTCTGGATAGGTTC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 333 bp | 110 aa | 67 | 399 | + | No |
Description : First part of the transposase
ORF sequence :
MRKPYPSDISREAFKEIEPLLLSGRKQTRPRVVDVYEIFCALLYILKSGCQWSMLPRDFPAKSTVHYYFSIWNKKPSETEPSILEQALKKCGWRGPYQQW
TERTNELYHH
TERTNELYHH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 537 bp | 178 aa | 332 | 868 | + | No |
Description : Second part of the transposase
ORF sequence :
KNVVGEARISNGRKEQTSFIIIDSQSVKNTDTARDKGYDAGKKVSGIKRHIIVDTQGLPHGILVTTADKTDRQGALDAITIHRDTLQNVEVVLVDGGYTG
EPFYLAVKNLIRARVEVAKRSELHKFAVIPQRWVVERSFAWLEKCRRLWKNCEKHLNTSLQFINLAFLRLRLHRKYSG
EPFYLAVKNLIRARVEVAKRSELHKFAVIPQRWVVERSFAWLEKCRRLWKNCEKHLNTSLQFINLAFLRLRLHRKYSG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 802 bp | 266 aa | 67 | 868 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKPYPSDISREAFKEIEPLLLSGRKQTRPRVVDVYEIFCALLYILKSGCQWSMLPRDFPAKSTVHYYFSIWNKKPSETEPSILEQALKNVVGEARISNG
RKEQTSFIIIDSQSVKNTDTARDKGYDAGKKVSGIKRHIIVDTQGLPHGILVTTADKTDRQGALDAITIHRDTLQNVEVVLVDGGYTGEPFYLAVKNLIR
ARVEVAKRSELHKFAVIPQRWVVERSFAWLEKCRRLWKNCEKHLNTSLQFINLAFLRLRLHRKYSG
RKEQTSFIIIDSQSVKNTDTARDKGYDAGKKVSGIKRHIIVDTQGLPHGILVTTADKTDRQGALDAITIHRDTLQNVEVVLVDGGYTGEPFYLAVKNLIR
ARVEVAKRSELHKFAVIPQRWVVERSFAWLEKCRRLWKNCEKHLNTSLQFINLAFLRLRLHRKYSG
Blast result :
Comments
The ORF has been reconstructed in silico. Translational slippage is likely to be responsible for creation of a stop codon in a run of seven bases of "A" (bases 330-336).
Protein sequence is 64% aa similar to that of ISISCARN82, 59% to ISCaa8 and 62% to ISNieu4.
A297 is the GC1 (global clone 1) reference strain.
Protein sequence is 64% aa similar to that of ISISCARN82, 59% to ISCaa8 and 62% to ISNieu4.
A297 is the GC1 (global clone 1) reference strain.
References
[1] Hamidian M., Ambrose S.J. and Hall R.M. (2016) Direct Submission.