ISAchd1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KT290950 ND Pseudomonas pseudoalcaligenes
Pseudomonas pseudoalcaligenes CECT 5344
Pseudomonas pseudoalcaligenes
Achromobacter denitrificans 6-2
DNA section
IS Length : 1027 bp

Ends


IR Length : 23/25

IRL : GGCTCTGTCTGAGTTATCTGTTGCAGTGGTCTAAACTGAATCGTGATCTT
IRR : GGCTATGTCTGAGTTAACTGTTGCATATCCCTATACCCCAACGCCTCTTT

Insertion site


Left flankDirect repeatRight flankDR Length
TCAACGACCTTTTGGAAT/ACGTCGGCTTC8
GAGGCATGAACGCTCCTTTTTTTGGCAATAGTGTTGATTT0
AAATAGTGATTTCCGTTTTGCCATTCCA8
CTGGAGGAGAAAGGAATTTGTCCCGCGA8
ATGGAGCGCATACGTTTTTGTGTGCGTA8
TTGGCCACGGTTTCCCTGGCGGTGGCGA8
TAGTGGCGCCGCATGCGTTTTTGCGAAAGGCCCCGAGCGTT0

DNA sequence

GGCTCTGTCTGAGTTATCTGTTGCAGTGGTCTAAACTGAATCGTGATCTTTCCCAGCATGAGGGATCTATCATGAAAGCGGTTCAGTTTTCGCGGTGGAT
GGCACAGCTCTCCAGCCTCAACCCAGAGCAACGCGACCAGTTGAGATCCAAGCTCTTGCCAGCTGCCAGGCACTGGCAGGACGCAATCAAGGCCCCAAGC
CATTGCCCGCATTGTCAATCGCGGGAACTGCGCCCCTGGGGTTCCAGTGGTGACTTGCCGCGATACCGCTGCAAGGTTTGCGGCAAAACCAGCAATGCCC
TGACGGGCACCCCAATGGCGCGACTGAGAAAGCGCCATCTCTGGCAAGACTATGCGGACGCACTGACCCAGAGCCTGAGCGTGCGCAAGGCCGCCGTTCA
TTGTGGCGTCAGCAAAAACACAGCTTTCCTGTGGCGACATAGGTTTCTTTCTCGGATTGCCGATCACCAGGCCCAGCACGCGTCAGGCATTGTTGAAGCA
GATGAAACCTTCTTCCTGGAGTCCTTCAAAGGGCAACGCGAACTGCCTCGACCGCCTCGTCGGCGCGGTGGCAGCGCCCAACGACGCGGACTGTCTGCCG
AGCAAATTCCTGTGCTGGTGGTAAGAGATCGCAGTGGTCAGCAGGCAGACTTCAAGTTGGAAAAGCTGGATGCACAGCACATAGGGGAGCGGCTACGCCC
GCTGATAGACGCGGATGCGATTCTCTGTACTGACAGCGCAAGTGTCTACGCCCATTTCGCCAAGGTTGAAGGCATCACTCACCGACCGATTAACCCGAGC
CAGAATCGACGGGTTGATGGTGCTTTCCACATCCAGAACGTGAATGCCTACGACAGCCGACTCAAGAGCTGGATGATTCCTTTTCATGGTGTGGCCACCA
AGTACCTAACGAACTATCTGGGCTGGCGCCGCCTGCTTGAGCGCTACAAAACACAGCTCAACCCATTGATTTGCTTGAAAGAGGCGTTGGGGTATAGGGA
TATGCAACAGTTAACTCAGACATAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
954 bp317 aa721025+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKAVQFSRWMAQLSSLNPEQRDQLRSKLLPAARHWQDAIKAPSHCPHCQSRELRPWGSSGDLPRYRCKVCGKTSNALTGTPMARLRKRHLWQDYADALTQ
SLSVRKAAVHCGVSKNTAFLWRHRFLSRIADHQAQHASGIVEADETFFLESFKGQRELPRPPRRRGGSAQRRGLSAEQIPVLVVRDRSGQQADFKLEKLD
AQHIGERLRPLIDADAILCTDSASVYAHFAKVEGITHRPINPSQNRRVDGAFHIQNVNAYDSRLKSWMIPFHGVATKYLTNYLGWRRLLERYKTQLNPLI
CLKEALGYRDMQQLTQT

 

Blast result :
Comments
ISAchd1 is 90% aa similar to ISAzvi1.
References
1] Andreas Wendel (2015) Direct submission
2] Wendel,A.F. and MacKenzie,C.R. (2015) Direct submission GenBank
3] Wibberg,Daniel. (2014) Direct submission GenBank.