ISAba33
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KT852972 | ND | Acinetobacter baumannii | Acinetobacter baumannii pMMD Acinetobacter baumannii isolate BAL_056; ENA accession ERR190414 Acinetobacter haemolyticus DNA |
DNA section
IS Length : 1180 bp
Ends
IR Length : 15/16
IRL : CTTTGTACACGACAAAAAAATATAACTCATTGAAATAATGGCATAATACT
IRR : CTCTGTACACGACAAATTTCATAGGACCCTTGTCCTATCAGGCTTTTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACTTTTTTT | ATTTTAAAC | AATTAAATTA | 9 |
DNA sequence
CTTTGTACACGACAAAAAAATATAACTCATTGAAATAATGGCATAATACTTGTTTTCTGAAGACGAATATGATGACACATTTCAATGAGTTATATCTCAT
CTTAAACAAATATCTAAAGTGGAACAAGTCACATGCAAAATGTTTTACACTGATTATGCTTGCATTAATTGTAAAACAGACATGTAATCTTTCTTCTGCA
TCTAAAGCCTTACCCATCAAGTGCTTACCACAATCATTTTATCGACGTATACAACGCTTCTTTGCAGGTCAGTATTTCGATTATCGTCAAATTTCTCAGT
TAATTTTCAATATATTTTCATTTGATAAAGTCCAATTAACTTTGGATAGAACTAATTGGAAATGGGGAAAACGAGATATCAATATCTTGATGTTAGCCAT
CGTCTATCGTGGAATAGCGATACCTATTGTTTGGACATTGCTCAATAAACGTGGAAATTCAGATACAAAAGAGCGTATTGCTTTGATTCAACGCTTTATC
TCCATTTTTGGTAAAGATCGTATTGTGAATGTGTTTGCAGACAGAGAATTTATCGGTGAGAAATGGTTTACATGGTTAATTGAAAATGACATTAACTTCT
GTATACGTGTTAAAAAAACTTTATTGTGACCAATCACTTAGCCAAGAATCATAAAATTAGTGATTTATTTCGTCATCTTCAAGTTGGTCAAACTGAATGT
CGTAAACGACGTATTTGGGTTGGTCGAGTGAAACTGTATATTAGTGCGCTACGATTAGAAGATGGAGAACTTTTACTTGTTGTTTCTCCTATGTTTAATG
CTTCTGCTATTCGTGATTATGCATTACGCTGGGAAATCGAAACGTTATTTAGTTGTCTCAAAGGACGTGGATTCAATCTTGAAAATACTCGTTTAACAGA
CCCTAGACGAGTCAAAAAATTGATAGCAGTGCTAGCTATCGGTTTCTGTTGGTGCTATTTAACAGGTGAATGGCAACATGATCGGAAAAAAGCGATAAAA
ATAAAGAAGCATGGACGACTTTCAGTAAGTTTATTTCGCTACGGTTTGGACTATGTTCAAATGGCTATTCTACGTTTGATTGGTTTTGGAAAAAAAGAAG
AATTTAAGAAAGTGCTAGCAATTTTAAGAAAGAAAAAGCCTGATAGGACAAGGGTCCTATGAAATTTGTCGTGTACAGAG
CTTAAACAAATATCTAAAGTGGAACAAGTCACATGCAAAATGTTTTACACTGATTATGCTTGCATTAATTGTAAAACAGACATGTAATCTTTCTTCTGCA
TCTAAAGCCTTACCCATCAAGTGCTTACCACAATCATTTTATCGACGTATACAACGCTTCTTTGCAGGTCAGTATTTCGATTATCGTCAAATTTCTCAGT
TAATTTTCAATATATTTTCATTTGATAAAGTCCAATTAACTTTGGATAGAACTAATTGGAAATGGGGAAAACGAGATATCAATATCTTGATGTTAGCCAT
CGTCTATCGTGGAATAGCGATACCTATTGTTTGGACATTGCTCAATAAACGTGGAAATTCAGATACAAAAGAGCGTATTGCTTTGATTCAACGCTTTATC
TCCATTTTTGGTAAAGATCGTATTGTGAATGTGTTTGCAGACAGAGAATTTATCGGTGAGAAATGGTTTACATGGTTAATTGAAAATGACATTAACTTCT
GTATACGTGTTAAAAAAACTTTATTGTGACCAATCACTTAGCCAAGAATCATAAAATTAGTGATTTATTTCGTCATCTTCAAGTTGGTCAAACTGAATGT
CGTAAACGACGTATTTGGGTTGGTCGAGTGAAACTGTATATTAGTGCGCTACGATTAGAAGATGGAGAACTTTTACTTGTTGTTTCTCCTATGTTTAATG
CTTCTGCTATTCGTGATTATGCATTACGCTGGGAAATCGAAACGTTATTTAGTTGTCTCAAAGGACGTGGATTCAATCTTGAAAATACTCGTTTAACAGA
CCCTAGACGAGTCAAAAAATTGATAGCAGTGCTAGCTATCGGTTTCTGTTGGTGCTATTTAACAGGTGAATGGCAACATGATCGGAAAAAAGCGATAAAA
ATAAAGAAGCATGGACGACTTTCAGTAAGTTTATTTCGCTACGGTTTGGACTATGTTCAAATGGCTATTCTACGTTTGATTGGTTTTGGAAAAAAAGAAG
AATTTAAGAAAGTGCTAGCAATTTTAAGAAAGAAAAAGCCTGATAGGACAAGGGTCCTATGAAATTTGTCGTGTACAGAG
Recoding section
- Recoding by frameshift
- Frame -1
- Type
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 558 bp | 185 aa | 72 | 629 | + | No |
Description : First part of the transposase
ORF sequence :
MTHFNELYLILNKYLKWNKSHAKCFTLIMLALIVKQTCNLSSASKALPIKCLPQSFYRRIQRFFAGQYFDYRQISQLIFNIFSFDKVQLTLDRTNWKWGK
RDINILMLAIVYRGIAIPIVWTLLNKRGNSDTKERIALIQRFISIFGKDRIVNVFADREFIGEKWFTWLIENDINFCIRVKKTLL
RDINILMLAIVYRGIAIPIVWTLLNKRGNSDTKERIALIQRFISIFGKDRIVNVFADREFIGEKWFTWLIENDINFCIRVKKTLL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 549 bp | 182 aa | 614 | 1162 | + | No |
Description : Second part of the transposase
ORF sequence :
KNFIVTNHLAKNHKISDLFRHLQVGQTECRKRRIWVGRVKLYISALRLEDGELLLVVSPMFNASAIRDYALRWEIETLFSCLKGRGFNLENTRLTDPRRV
KKLIAVLAIGFCWCYLTGEWQHDRKKAIKIKKHGRLSVSLFRYGLDYVQMAILRLIGFGKKEEFKKVLAILRKKKPDRTRVL
KKLIAVLAIGFCWCYLTGEWQHDRKKAIKIKKHGRLSVSLFRYGLDYVQMAILRLIGFGKKEEFKKVLAILRKKKPDRTRVL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1091 bp | 363 aa | 72 | 1162 | + | Yes |
Chemistry : DDE
ORF sequence :
MTHFNELYLILNKYLKWNKSHAKCFTLIMLALIVKQTCNLSSASKALPIKCLPQSFYRRIQRFFAGQYFDYRQISQLIFNIFSFDKVQLTLDRTNWKWGK
RDINILMLAIVYRGIAIPIVWTLLNKRGNSDTKERIALIQRFISIFGKDRIVNVFADREFIGEKWFTWLIENDINFCIRVKKNFIVTNHLAKNHKISDLF
RHLQVGQTECRKRRIWVGRVKLYISALRLEDGELLLVVSPMFNASAIRDYALRWEIETLFSCLKGRGFNLENTRLTDPRRVKKLIAVLAIGFCWCYLTGE
WQHDRKKAIKIKKHGRLSVSLFRYGLDYVQMAILRLIGFGKKEEFKKVLAILRKKKPDRTRVL
RDINILMLAIVYRGIAIPIVWTLLNKRGNSDTKERIALIQRFISIFGKDRIVNVFADREFIGEKWFTWLIENDINFCIRVKKNFIVTNHLAKNHKISDLF
RHLQVGQTECRKRRIWVGRVKLYISALRLEDGELLLVVSPMFNASAIRDYALRWEIETLFSCLKGRGFNLENTRLTDPRRVKKLIAVLAIGFCWCYLTGE
WQHDRKKAIKIKKHGRLSVSLFRYGLDYVQMAILRLIGFGKKEEFKKVLAILRKKKPDRTRVL
Blast result :
Comments
ISAba33 is 87% identity with ISAba1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba33 was found in a Tn2008-like transposon (which has been submitted to NCBI genbank under accession KT852972).
Host: Acinetobacter baumannii; Ventilator associated pneumonia sample; isolate BAL_056; ENA accession ERR190414; collection date 16-Mar-2009.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba33 was found in a Tn2008-like transposon (which has been submitted to NCBI genbank under accession KT852972).
Host: Acinetobacter baumannii; Ventilator associated pneumonia sample; isolate BAL_056; ENA accession ERR190414; collection date 16-Mar-2009.
References
1] Schultz, M. B. (2015) Direct submission
2] Schultz, M. B., Thanh, D. P., Hoang, N. T. D., Wick, R. R., Ingle, D. J., Hawkey, J., Edwards, D. J., Kenyon, J. J., Lan, N. P. H., Campbell, J. I., Thwaites, G., Nhu, N. T. K., Hall, R. M., Fournier-Level, A., Baker, S., Holt, K. E. (2015) Microbial Genomics.
2] Schultz, M. B., Thanh, D. P., Hoang, N. T. D., Wick, R. R., Ingle, D. J., Hawkey, J., Edwards, D. J., Kenyon, J. J., Lan, N. P. H., Campbell, J. I., Thwaites, G., Nhu, N. T. K., Hall, R. M., Fournier-Level, A., Baker, S., Holt, K. E. (2015) Microbial Genomics.