ISAeca4
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Aeromonas caviae | Aeromonas caviae |
DNA section
IS Length : 1235 bp
Ends
IR Length : 23/28
IRL : TGAATCGCCCCTACTTTCCTAGACACCTCAAGAGCCTCAAACTGAGGTCA
IRR : TGAATCGCCCCGGCTTCTCTAGACACTTTTGAGCCGTTCGAAATATTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCACCGAAA | CTTTTTTGTG | 0 |
DNA sequence
TGAATCGCCCCTACTTTCCTAGACACCTCAAGAGCCTCAAACTGAGGTCAATCAGGAGGTGTTATGAGCAAGCAGCAACGTTACACGGAAGAATTCAAAC
TGGCCGCCGTCAAGCAGGTGACTGAGCGCCATTACCCAGTGGCAGAGGTCGCTGAGCGACTTGGTGTTTCCAGCCACAGTCTTTACGCCTGGATCAGGCG
CTACAGCCAGCCCGCCGTGCAGCAACAGCATGACTCCAGCCAGCAAGCCGAAATTCGGCGCCTCAAGGCTGAACTGCGCCGCGTCACCGATGAGCGAGAT
ATCTTAAAAAAGGCCGCCGCGTACTTTGCCAAGCTGTCAGGGTAAGGTACGCCTTCATCCGGGTGCACGCGTCCACACATCCCATCCGCCGACTGTGTCG
GATGATGAATGTCCACCCGAGCGGTTATTACGCCTGGCAGCACCAGCCTTTATCTGCCCGCGCACTTGAAGACCAACGCCTCCTGGGCCAGATCAAGCAA
TGTTGGCTAGAAAGCGGGGGCGTCTATGGCTATCGCAAGATCCGGGATGACCTGCGAGAGATGGGCGAGACCTGTGGGAAACACCGGGTGGCGCGATTAA
TGCGTCATGAAGGTTTGCGTTCACAAACCGGTTATCGTCGCCGTCCCGGTCATTATGGTGGCCGGCCAACGGTGGTTGCACCGAATCATCTTGAACGCCA
GTTCGATGTGACTGAGCCAAATAAGGTCTGGGTAACGGACATCACTTATATTCGAACCCATGAAGGCTGGCTCTATCTGGCCGCGGTATTGGATTTGTTT
TCACGACAAGTCATTGGTTGGTCAATGGGGCCACGGATCGACCGAGAGTTGGCCATCAGCGCGTTACTGATGGCAGTGTGGCGACGGCGACCCGAGCAGG
AAGTGCTGGTTCATTCTGACCAAGGGAGCCAGTTTAGCAGCTATGACTGGCAGGATTTTTTGCGGGAGCACCGGCTCAAGGCCAGCATGAGCAGACGTGG
CAATTGCCATGATAACGCGGTGGCGGAGAGTTTCTTCCAACTCCTCAAACGCGAGCGGATCCGACGTAAAACCTATGCAGATCGGGAGGAGGCCCAGCAG
GATGTGTTCGATTACATTGAAATGTTTTACAACCCCAAGCGTCGGCACAGTTTCAACAATGGGCTATCGCCAGTAGAGTGTGAAAAGCAATATTTCGAAC
GGCTCAAAAGTGTCTAGAGAAGCCGGGGCGATTCA
TGGCCGCCGTCAAGCAGGTGACTGAGCGCCATTACCCAGTGGCAGAGGTCGCTGAGCGACTTGGTGTTTCCAGCCACAGTCTTTACGCCTGGATCAGGCG
CTACAGCCAGCCCGCCGTGCAGCAACAGCATGACTCCAGCCAGCAAGCCGAAATTCGGCGCCTCAAGGCTGAACTGCGCCGCGTCACCGATGAGCGAGAT
ATCTTAAAAAAGGCCGCCGCGTACTTTGCCAAGCTGTCAGGGTAAGGTACGCCTTCATCCGGGTGCACGCGTCCACACATCCCATCCGCCGACTGTGTCG
GATGATGAATGTCCACCCGAGCGGTTATTACGCCTGGCAGCACCAGCCTTTATCTGCCCGCGCACTTGAAGACCAACGCCTCCTGGGCCAGATCAAGCAA
TGTTGGCTAGAAAGCGGGGGCGTCTATGGCTATCGCAAGATCCGGGATGACCTGCGAGAGATGGGCGAGACCTGTGGGAAACACCGGGTGGCGCGATTAA
TGCGTCATGAAGGTTTGCGTTCACAAACCGGTTATCGTCGCCGTCCCGGTCATTATGGTGGCCGGCCAACGGTGGTTGCACCGAATCATCTTGAACGCCA
GTTCGATGTGACTGAGCCAAATAAGGTCTGGGTAACGGACATCACTTATATTCGAACCCATGAAGGCTGGCTCTATCTGGCCGCGGTATTGGATTTGTTT
TCACGACAAGTCATTGGTTGGTCAATGGGGCCACGGATCGACCGAGAGTTGGCCATCAGCGCGTTACTGATGGCAGTGTGGCGACGGCGACCCGAGCAGG
AAGTGCTGGTTCATTCTGACCAAGGGAGCCAGTTTAGCAGCTATGACTGGCAGGATTTTTTGCGGGAGCACCGGCTCAAGGCCAGCATGAGCAGACGTGG
CAATTGCCATGATAACGCGGTGGCGGAGAGTTTCTTCCAACTCCTCAAACGCGAGCGGATCCGACGTAAAACCTATGCAGATCGGGAGGAGGCCCAGCAG
GATGTGTTCGATTACATTGAAATGTTTTACAACCCCAAGCGTCGGCACAGTTTCAACAATGGGCTATCGCCAGTAGAGTGTGAAAAGCAATATTTCGAAC
GGCTCAAAAGTGTCTAGAGAAGCCGGGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 64 | 345 | + | No |
Description : First part of the transposase
ORF sequence :
MSKQQRYTEEFKLAAVKQVTERHYPVAEVAERLGVSSHSLYAWIRRYSQPAVQQQHDSSQQAEIRRLKAELRRVTDERDILKKAAAYFAKLSG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 294 | 1217 | + | No |
Description : Second part of the transposase
ORF sequence :
ARYLKKGRRVLCQAVRVRYAFIRVHASTHPIRRLCRMMNVHPSGYYAWQHQPLSARALEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVA
RLMRHEGLRSQTGYRRRPGHYGGRPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAAVLDLFSRQVIGWSMGPRIDRELAISALLMAVWRRRP
EQEVLVHSDQGSQFSSYDWQDFLREHRLKASMSRRGNCHDNAVAESFFQLLKRERIRRKTYADREEAQQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQY
FERLKSV
RLMRHEGLRSQTGYRRRPGHYGGRPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAAVLDLFSRQVIGWSMGPRIDRELAISALLMAVWRRRP
EQEVLVHSDQGSQFSSYDWQDFLREHRLKASMSRRGNCHDNAVAESFFQLLKRERIRRKTYADREEAQQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQY
FERLKSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1154 bp | 384 aa | 64 | 1217 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKQQRYTEEFKLAAVKQVTERHYPVAEVAERLGVSSHSLYAWIRRYSQPAVQQQHDSSQQAEIRRLKAELRRVTDERDILKKGRRVLCQAVRVRYAFIR
VHASTHPIRRLCRMMNVHPSGYYAWQHQPLSARALEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVARLMRHEGLRSQTGYRRRPGHYGG
RPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAAVLDLFSRQVIGWSMGPRIDRELAISALLMAVWRRRPEQEVLVHSDQGSQFSSYDWQDFL
REHRLKASMSRRGNCHDNAVAESFFQLLKRERIRRKTYADREEAQQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV
VHASTHPIRRLCRMMNVHPSGYYAWQHQPLSARALEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVARLMRHEGLRSQTGYRRRPGHYGG
RPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAAVLDLFSRQVIGWSMGPRIDRELAISALLMAVWRRRPEQEVLVHSDQGSQFSSYDWQDFL
REHRLKASMSRRGNCHDNAVAESFFQLLKRERIRRKTYADREEAQQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV
Blast result :
Comments
ISAeca4 is 84% aa similar to ISPsy29.
IS_pep_1 is DA11_14995.
IS_pep_1 is DA11_14995.
References
1] Pfeiffer F. (2015) Direct submission