ISAs34
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000644 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 |
DNA section
IS Length : 928 bp
Ends
IR Length : 18/21
IRL : GGCTGTGTTCTTAAAGTGATGAAGATATAATCTCACATCTTCTCAACAGC
IRR : GGCTGTGTTCTCAAAATGCTGGTTTTCTGTCCACCAATAGCTCCCGTAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATCTGAGAC | TA | AACCAAGGGC | 2 |
DNA sequence
GGCTGTGTTCTTAAAGTGATGAAGATATAATCTCACATCTTCTCAACAGCTTAAGACCTATGCAGTGTCCCGTCGTTACCAGTTGACCGATGCACAATGG
GGACTTATCGAGCCCCTTTTTCCATACCACCGCATAAGCGGACGTCCTACTCGCGAGCCACGCCAAGTGCTCGATGGCATTCTCTGGATACTCCACACCG
GTGCGCCTTGGCGAGATCTGCCAGAACGTTTCGGCCTATGGAGTTCGGTCTACAACACCTTTCGACGTTGGCAGAGTACAGGTCACATCGATGCCATCCT
TGAAGCGCTGCAACTACATCTCAATGAAGCAGGGCTTATCGACTTCGACCTGTGGTGCATTGACGGCTCCAATGTCAGGGCATCCAAAGATGCTGCCGGA
GCGCGTAAAAAAACACTCTTGTGAATCAAGCGCTTGGTCGCTCGCGTGGAGGTTTTGGCAGCAAAATTCACTTGGTCACTGATGGTAATGGTTTACCGCT
GGGGTTCTGCCTTTCACCGGGGCAATCAGCGGAAATCAGATATGCGACAAGTGCGCTGGCCATGGCAAGGATACCGACCTCATCAGGCCGTTATCGCACG
CGTCCAGCGCATCTTGCCGCAGATAAGGCCTATAGCAGCAGGGCTTTGCGGGCCGAACTCCGGCGCAGAAGGATAAAAGCAGTCATCCCGCAGCGCAGCG
ACCAGCAGCGGCACCATAAGGGACGTCCTCTGGTATTAGACAAAGCTCGTTATCGCAGGCGAAATGTGGTCGAGCGGTGCTTCGGATGGCTGAAGAAGTT
TCGCCGATTCTCAACGAGATATGAAAAGCTGGCGGGAAGTTTTGCTGCTTTTATCAAGCTGGCTTTCTGCCTTCGTTACTTACGGGAGCTATTGGTGGAC
AGAAAACCAGCATTTTGAGAACACAGCC
GGACTTATCGAGCCCCTTTTTCCATACCACCGCATAAGCGGACGTCCTACTCGCGAGCCACGCCAAGTGCTCGATGGCATTCTCTGGATACTCCACACCG
GTGCGCCTTGGCGAGATCTGCCAGAACGTTTCGGCCTATGGAGTTCGGTCTACAACACCTTTCGACGTTGGCAGAGTACAGGTCACATCGATGCCATCCT
TGAAGCGCTGCAACTACATCTCAATGAAGCAGGGCTTATCGACTTCGACCTGTGGTGCATTGACGGCTCCAATGTCAGGGCATCCAAAGATGCTGCCGGA
GCGCGTAAAAAAACACTCTTGTGAATCAAGCGCTTGGTCGCTCGCGTGGAGGTTTTGGCAGCAAAATTCACTTGGTCACTGATGGTAATGGTTTACCGCT
GGGGTTCTGCCTTTCACCGGGGCAATCAGCGGAAATCAGATATGCGACAAGTGCGCTGGCCATGGCAAGGATACCGACCTCATCAGGCCGTTATCGCACG
CGTCCAGCGCATCTTGCCGCAGATAAGGCCTATAGCAGCAGGGCTTTGCGGGCCGAACTCCGGCGCAGAAGGATAAAAGCAGTCATCCCGCAGCGCAGCG
ACCAGCAGCGGCACCATAAGGGACGTCCTCTGGTATTAGACAAAGCTCGTTATCGCAGGCGAAATGTGGTCGAGCGGTGCTTCGGATGGCTGAAGAAGTT
TCGCCGATTCTCAACGAGATATGAAAAGCTGGCGGGAAGTTTTGCTGCTTTTATCAAGCTGGCTTTCTGCCTTCGTTACTTACGGGAGCTATTGGTGGAC
AGAAAACCAGCATTTTGAGAACACAGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 360 bp | 119 aa | 65 | 424 | + | No |
Description : First part of the transposase
ORF sequence :
VSRRYQLTDAQWGLIEPLFPYHRISGRPTREPRQVLDGILWILHTGAPWRDLPERFGLWSSVYNTFRRWQSTGHIDAILEALQLHLNEAGLIDFDLWCID
GSNVRASKDAAGARKKTLL
GSNVRASKDAAGARKKTLL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 409 | 918 | + | No |
Description : Second part of the transposase
ORF sequence :
KNTLVNQALGRSRGGFGSKIHLVTDGNGLPLGFCLSPGQSAEIRYATSALAMARIPTSSGRYRTRPAHLAADKAYSSRALRAELRRRRIKAVIPQRSDQQ
RHHKGRPLVLDKARYRRRNVVERCFGWLKKFRRFSTRYEKLAGSFAAFIKLAFCLRYLRELLVDRKPAF
RHHKGRPLVLDKARYRRRNVVERCFGWLKKFRRFSTRYEKLAGSFAAFIKLAFCLRYLRELLVDRKPAF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 854 bp | 284 aa | 65 | 918 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRRYQLTDAQWGLIEPLFPYHRISGRPTREPRQVLDGILWILHTGAPWRDLPERFGLWSSVYNTFRRWQSTGHIDAILEALQLHLNEAGLIDFDLWCID
GSNVRASKDAAGARKKNTLVNQALGRSRGGFGSKIHLVTDGNGLPLGFCLSPGQSAEIRYATSALAMARIPTSSGRYRTRPAHLAADKAYSSRALRAELR
RRRIKAVIPQRSDQQRHHKGRPLVLDKARYRRRNVVERCFGWLKKFRRFSTRYEKLAGSFAAFIKLAFCLRYLRELLVDRKPAF
GSNVRASKDAAGARKKNTLVNQALGRSRGGFGSKIHLVTDGNGLPLGFCLSPGQSAEIRYATSALAMARIPTSSGRYRTRPAHLAADKAYSSRALRAELR
RRRIKAVIPQRSDQQRHHKGRPLVLDKARYRRRNVVERCFGWLKKFRRFSTRYEKLAGSFAAFIKLAFCLRYLRELLVDRKPAF
Blast result :
Comments
ISAs34 is 64% aa similar to ISPst8.
IS_pep_1 is ASA_0455 which starts with the GTG for Val-120 in UniProt.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
IS_pep_1 is ASA_0455 which starts with the GTG for Val-120 in UniProt.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S. and Brown L. (2007) Direct submission to GenBank
2] Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S. and Brown L. (2007) Direct submission to GenBank