ISAs33
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000644 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 |
DNA section
IS Length : 1239 bp
Ends
IR Length : 21/26
IRL : TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTACAGTCGCCAC
IRR : TGTAGTGGTCAACTAAATCCGGACACTTGGTTAGGCCGAGTCTCGGCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTGGTATTA | CTTGCTCGCT | 0 |
DNA sequence
TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTACAGTCGCCACCATGACCTGAGGTGTTCAATGACAAGATTACGTCGCTCATTTACTGCCGA
ATTCAAACTCGAAGCCGCATCCCTGGTGCTCGACCAGGGGTATTCTGTTCCCGAAGCAAGCCGTTCACTGGATGTCGGTGAAACCGTGCTGCGCCGATGG
GTTCAGCAACTGCAATCCGAACGCAGTGGCACAACCCCAATCAGCAAAGCACTGACTCCGGAACAGCAGAAAATTCAGGAGCTGGAAGCCCGTATCAACC
GGCTCGAACGTGAAAAGGACATACTCAAAAAGGCTACTGCTCTCTTGATGACGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACCCTGGTCTGCTGCGCTTTCGATATTCCCACATCCTGTTTTTACGATTATCTGGCTCGCAGGAGAGTGATTAACCGTGAGCGAATGCAACAGC
GCAGTGAGTTGCACCGCTTGTTCACAGCGAGCCGGGACTCGGCAGGCAGCCGTGCCTTGATGTCGATGATGCGAGAGCTGGGGTATCAGATTGGCCGATT
CAAAGTCCGTAACCTGATGAGGGAAGCCGGGTTGGCATCCAAACAGCCGGGCACACATCGTTATAAGGTTGCACGGTCTGAACGACCGGATATTCCGAAT
CTGCTGGCCCGCGAATTTGATGTTCCGCAACCCAATCAAGTGTGGTGTGGCGATATCACCTACGTCTGGGCAGGTACTCGTTGGCATTACCTAGCTGTGG
TTCTTGACCTCCATACTCGCCGAGTCGTGGGCTGGGCAATGTCTGACAAGCCTGATGCAGAGCTGGCAATCAAAGCACTGGAGATGGCCTATCAGCAGCG
GGGTTGCCCATCCGGTGTGCTGTTTCACTCCGACCAGGGTAGCCAATATGGCAGCAGGGCATTTCGGCAACGACTGTGGCGCTATCGCATGACCCAGAGC
ATGAGTCGGCGTGGCAACTGCTGGGATAACGCCCCGATGGAGCGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTCCCGGCAACGGGATACATGAGCCTGC
GGGAAGCAACGCGGGATATCAGTTATTACCTGATGGATTACTACAACTGGCGGCGTCCACATCAACACAATGACGGGATGCCGCCAGCAAAAGCCGAGAC
TCGGCCTAACCAAGTGTCCGGATTTAGTTGACCACTACA
ATTCAAACTCGAAGCCGCATCCCTGGTGCTCGACCAGGGGTATTCTGTTCCCGAAGCAAGCCGTTCACTGGATGTCGGTGAAACCGTGCTGCGCCGATGG
GTTCAGCAACTGCAATCCGAACGCAGTGGCACAACCCCAATCAGCAAAGCACTGACTCCGGAACAGCAGAAAATTCAGGAGCTGGAAGCCCGTATCAACC
GGCTCGAACGTGAAAAGGACATACTCAAAAAGGCTACTGCTCTCTTGATGACGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACCCTGGTCTGCTGCGCTTTCGATATTCCCACATCCTGTTTTTACGATTATCTGGCTCGCAGGAGAGTGATTAACCGTGAGCGAATGCAACAGC
GCAGTGAGTTGCACCGCTTGTTCACAGCGAGCCGGGACTCGGCAGGCAGCCGTGCCTTGATGTCGATGATGCGAGAGCTGGGGTATCAGATTGGCCGATT
CAAAGTCCGTAACCTGATGAGGGAAGCCGGGTTGGCATCCAAACAGCCGGGCACACATCGTTATAAGGTTGCACGGTCTGAACGACCGGATATTCCGAAT
CTGCTGGCCCGCGAATTTGATGTTCCGCAACCCAATCAAGTGTGGTGTGGCGATATCACCTACGTCTGGGCAGGTACTCGTTGGCATTACCTAGCTGTGG
TTCTTGACCTCCATACTCGCCGAGTCGTGGGCTGGGCAATGTCTGACAAGCCTGATGCAGAGCTGGCAATCAAAGCACTGGAGATGGCCTATCAGCAGCG
GGGTTGCCCATCCGGTGTGCTGTTTCACTCCGACCAGGGTAGCCAATATGGCAGCAGGGCATTTCGGCAACGACTGTGGCGCTATCGCATGACCCAGAGC
ATGAGTCGGCGTGGCAACTGCTGGGATAACGCCCCGATGGAGCGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTCCCGGCAACGGGATACATGAGCCTGC
GGGAAGCAACGCGGGATATCAGTTATTACCTGATGGATTACTACAACTGGCGGCGTCCACATCAACACAATGACGGGATGCCGCCAGCAAAAGCCGAGAC
TCGGCCTAACCAAGTGTCCGGATTTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 69 | 377 | + | No |
Description : First part of the transposase
ORF sequence :
MTRLRRSFTAEFKLEAASLVLDQGYSVPEASRSLDVGETVLRRWVQQLQSERSGTTPISKALTPEQQKIQELEARINRLEREKDILKKATALLMTDEFTR
TR
TR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 314 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
KGHTQKGYCSLDDGRVHTYALIDRLREQAPITLVCCAFDIPTSCFYDYLARRRVINRERMQQRSELHRLFTASRDSAGSRALMSMMRELGYQIGRFKVRN
LMREAGLASKQPGTHRYKVARSERPDIPNLLAREFDVPQPNQVWCGDITYVWAGTRWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPS
GVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYMSLREATRDISYYLMDYYNWRRPHQHNDGMPPAKAETRPNQ
VSGFS
LMREAGLASKQPGTHRYKVARSERPDIPNLLAREFDVPQPNQVWCGDITYVWAGTRWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPS
GVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYMSLREATRDISYYLMDYYNWRRPHQHNDGMPPAKAETRPNQ
VSGFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 69 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRLRRSFTAEFKLEAASLVLDQGYSVPEASRSLDVGETVLRRWVQQLQSERSGTTPISKALTPEQQKIQELEARINRLEREKDILKKGYCSLDDGRVHT
YALIDRLREQAPITLVCCAFDIPTSCFYDYLARRRVINRERMQQRSELHRLFTASRDSAGSRALMSMMRELGYQIGRFKVRNLMREAGLASKQPGTHRYK
VARSERPDIPNLLAREFDVPQPNQVWCGDITYVWAGTRWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYMSLREATRDISYYLMDYYNWRRPHQHNDGMPPAKAETRPNQVSGFS
YALIDRLREQAPITLVCCAFDIPTSCFYDYLARRRVINRERMQQRSELHRLFTASRDSAGSRALMSMMRELGYQIGRFKVRNLMREAGLASKQPGTHRYK
VARSERPDIPNLLAREFDVPQPNQVWCGDITYVWAGTRWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYMSLREATRDISYYLMDYYNWRRPHQHNDGMPPAKAETRPNQVSGFS
Blast result :
Comments
ISAs33 is 96% aa similar to ISAs6.
IS_pep_1 is ASA_1678.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
IS_pep_1 is ASA_1678.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission to GenBank
2] Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S. and Brown L. (2007) Direct submission to GenBank
2] Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S. and Brown L. (2007) Direct submission to GenBank