ISAs32
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000644 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 |
DNA section
IS Length : 1232 bp
Ends
IR Length : 22/26
IRL : TGAATCGCCCCCAGTTCCCTAGACACCTCGCAGCCTTAAAATGAGGCAAC
IRR : TGAATCGCCCCGGGTTTTCTAGACACTCTGTTGTTGAACATTGAACTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAAAAGGAC | CTGGCATCCA | 0 |
DNA sequence
TGAATCGCCCCCAGTTCCCTAGACACCTCGCAGCCTTAAAATGAGGCAACTATTGGAGGTGTTATGACCCGCCAGCATTACCCAGAAACATTCAAGATAG
AAGCGGTTAAACAAGTCACCGAGCGCGGGCGGCCTGTGGCTGATGTTGCACGAGCCCTCGGGGTATCCAGCCACAGTCTGTATGCCTGGCTCAAGCAGTA
CGGTCAGCCCGCTGACCAGCAGCACGAAGAGTCTGCCTTGCAAGCCGAGATACGCCGTCTGAAAGCCGAACTGCGCCAAGTCACCGAGGAGCGCAACATT
TTAAAGGAGGCCGCAGCCTACTTTGCCAGCGAGTCAAGGAAAGGTACGCGTTCATAACCTCCCGACTCGACCGGTACAGTGTCGTTACCCTGTGCCAGAC
CCCGGGGGTTCACCGAAGTGGCTTCTATGCGTGGTTGAAACAGCCCGCGTCGCCACGCCAGCAGGAGGACGAACGACTGACCAGATTGGTCAAGCAATCC
TGGCTGGAAAGTGGTGCTGTCTACGGTTATCGCAAGATCCATCGTGACCTTCGTGACTGGGGGGAAGCCTGCGGCAAGCACCGTGTGGCCAAATTAATGA
GGCGAGAAGGACTGCGTTCGCAAACCGGCTATCAGCGCCGTAAGGGGCATTATGGGGGTAAGAGCCTGCAGGCTGCTCCGAACATGCGGGCTCGTCAGTT
TAAGACCCCAGCCCCCAATATCTCCTGGGTGACCGACATTACCTACATCCGCACTCAAGAAGGGTGGCTGTATCTGGCTATAGTGCTAGATCTGTTTTCT
CGCCAGATCGTCGGCTGGGCGATGAAGTCACGCATGACGGCAGACTTGGCGGTTGATGCGTTATGGATGGCGGTTTGGCGGCACAAGCCAAAGCAGCCGG
TACTGGTGCATTCGGATCAGGGCAGTCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCCCACAACCTGAGTTGCAGTATGAGCCTACGTGGTAA
CTGTCACGACAACGCTGTCGCCGAGAGCTTCTTTCAGTTACTCAAACGGGAGCGGATCAAGCGCAGGATTTACCCGACAAAAGACGAAGCCCGCAGCGAC
GTATTTGATTATATCGAGATGTTTTACAACCCAATCAGGCGCCATGGTTCCAATGATGGGCTGTCGCCGGTAGAGTTTGAAAAGCAGTTCAATGTTCAAC
AACAGAGTGTCTAGAAAACCCGGGGCGATTCA
AAGCGGTTAAACAAGTCACCGAGCGCGGGCGGCCTGTGGCTGATGTTGCACGAGCCCTCGGGGTATCCAGCCACAGTCTGTATGCCTGGCTCAAGCAGTA
CGGTCAGCCCGCTGACCAGCAGCACGAAGAGTCTGCCTTGCAAGCCGAGATACGCCGTCTGAAAGCCGAACTGCGCCAAGTCACCGAGGAGCGCAACATT
TTAAAGGAGGCCGCAGCCTACTTTGCCAGCGAGTCAAGGAAAGGTACGCGTTCATAACCTCCCGACTCGACCGGTACAGTGTCGTTACCCTGTGCCAGAC
CCCGGGGGTTCACCGAAGTGGCTTCTATGCGTGGTTGAAACAGCCCGCGTCGCCACGCCAGCAGGAGGACGAACGACTGACCAGATTGGTCAAGCAATCC
TGGCTGGAAAGTGGTGCTGTCTACGGTTATCGCAAGATCCATCGTGACCTTCGTGACTGGGGGGAAGCCTGCGGCAAGCACCGTGTGGCCAAATTAATGA
GGCGAGAAGGACTGCGTTCGCAAACCGGCTATCAGCGCCGTAAGGGGCATTATGGGGGTAAGAGCCTGCAGGCTGCTCCGAACATGCGGGCTCGTCAGTT
TAAGACCCCAGCCCCCAATATCTCCTGGGTGACCGACATTACCTACATCCGCACTCAAGAAGGGTGGCTGTATCTGGCTATAGTGCTAGATCTGTTTTCT
CGCCAGATCGTCGGCTGGGCGATGAAGTCACGCATGACGGCAGACTTGGCGGTTGATGCGTTATGGATGGCGGTTTGGCGGCACAAGCCAAAGCAGCCGG
TACTGGTGCATTCGGATCAGGGCAGTCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCCCACAACCTGAGTTGCAGTATGAGCCTACGTGGTAA
CTGTCACGACAACGCTGTCGCCGAGAGCTTCTTTCAGTTACTCAAACGGGAGCGGATCAAGCGCAGGATTTACCCGACAAAAGACGAAGCCCGCAGCGAC
GTATTTGATTATATCGAGATGTTTTACAACCCAATCAGGCGCCATGGTTCCAATGATGGGCTGTCGCCGGTAGAGTTTGAAAAGCAGTTCAATGTTCAAC
AACAGAGTGTCTAGAAAACCCGGGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 64 | 357 | + | No |
Description : First part of the transposase
ORF sequence :
MTRQHYPETFKIEAVKQVTERGRPVADVARALGVSSHSLYAWLKQYGQPADQQHEESALQAEIRRLKAELRQVTEERNILKEAAAYFASESRKGTRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 999 bp | 332 aa | 216 | 1214 | + | No |
Description : Second part of the transposase
ORF sequence :
PAARRVCLASRDTPSESRTAPSHRGAQHFKGGRSLLCQRVKERYAFITSRLDRYSVVTLCQTPGVHRSGFYAWLKQPASPRQQEDERLTRLVKQSWLESG
AVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSLQAAPNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVG
WAMKSRMTADLAVDALWMAVWRHKPKQPVLVHSDQGSQFTGGEWQDFLKAHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYI
EMFYNPIRRHGSNDGLSPVEFEKQFNVQQQSV
AVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSLQAAPNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVG
WAMKSRMTADLAVDALWMAVWRHKPKQPVLVHSDQGSQFTGGEWQDFLKAHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYI
EMFYNPIRRHGSNDGLSPVEFEKQFNVQQQSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 64 | 1214 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRQHYPETFKIEAVKQVTERGRPVADVARALGVSSHSLYAWLKQYGQPADQQHEESALQAEIRRLKAELRQVTEERNILKGGRSLLCQRVKERYAFITS
RLDRYSVVTLCQTPGVHRSGFYAWLKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGK
SLQAAPNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHSDQGSQFTGGEWQDFLK
AHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFEKQFNVQQQSV
RLDRYSVVTLCQTPGVHRSGFYAWLKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGK
SLQAAPNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHSDQGSQFTGGEWQDFLK
AHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFEKQFNVQQQSV
Blast result :
Comments
ISAs32 is 94% aa similar to ISAs7.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S. and Brown L. (2007) Direct submission to GenBank
2] Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S. and Brown L. (2007) Direct submission to GenBank