ISfinder Mirror Site

ISPsy34

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP011972	ND	Pseudomonas syringae	Pseudomonas syringae pv. actinidiae ICMP 18884

DNA section

IS Length : 2041 bp

Ends

IR Length : 22/32

IRL : GTAAGCGCCTCATGAACCCCACATTCCAAACAGACAATTGAAGCCTGATC
IRR : GTATCCGCGCCACAAACCCCACCTGGCAAAAAATGAGGATCTGGTTTACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCACCTTTCTTT	CCAGCATC	GTCAGCAAACTG	8
AATCGATAATGA	GAAAAACC	CTACAGCAAGGT	8
ACCTTGAGGGGC	GGTTGATC	AGCGCTATCGAG	8

DNA sequence

GTAAGCGCCTCATGAACCCCACATTCCAAACAGACAATTGAAGCCTGATCCTTTGCTCCCGATTCTTTTCAGGAGCCAGCATCCCCATGATGCGACCCGA
CGCCAAAGTCGAAAAAGTGTACCTCTACCCCAAGCCTGTGGATTTCCGAAAATCCATCGATGGCCTTGCTGCGCTGGTCGAGCTGGATATCAAAGTCGCT
GTGTTCGACCCCGTGCTTTTTGTTTTTCTGAATCGCCACCGTAACCGGGTGAAAATCCTTTATTGGGAGCGCAACGGCTTCTGCCTTTGGCTCAAGCGCC
TGGAGTCCGAGCGGTTCAAAACCTCTCCCGATGAGACGGACGAGGCCATCGTGCTGACAGTCCAGGAACTGAACTGGTTACTGGATGGATTTGATCTCTG
GCGTAACCGTCCGCACAAGGTTTTGACTCCACGATTTGTCGCCTAACGGGTATAATCCAGGGCATGATTTCCATGCCCGAAGATCTTCCAGATGACCCTG
TATTGCTCAAGCAACTGCTCGCGCAGTTACTGATTGAGCGCACGGCTGATAAGGGGCATATCGTCGATCTCAAAGAACAGATCAAGTTACTGCGCGACCG
TCTGTTCGGACGTAAGTCCGAACAGACGGTCGAACCCAACACGCCGCAACTGGCACTGTTCAACGAACCCGAAAGCGAGCCGATGCTTCCCGTCGGCGAC
GCCGAAGAAGAGGTCGTTGCACCGGTCAAACGCCGTGGTAAACGCAAACCCCTGTCGGCTGATCTTCCCCGCATCGAAGTCATTCACGAACTGCCCGAGC
ACGAACTGACCTGTGCCTGCGGTTGCCGCAAGCATGTCATCAGCGAAGAAACCAGCGAGCAGCTGGATATCGTGCCAATGCAGATCCGTGTGATCAAACA
CATCCGCAAAGTTTACGGTTGCCGCAGTTGCGAAACAGCACCGGTCACCGCGGACAAACCGGCTCAATTGATCGAAAAGAGCATGGCCAGTCCGAGCGTA
CTGGCGATGCTGCTGACCACTAAATATGTCGACGGCTTGCCTCTGCATCGTTTCGAAACGGTGCTGAGTCGACATGGCATCGAGATACCGCGCCAAACTT
TGGCACGCTGGGTAATCCAGTGCAGCGAGCACTTCCAGCCGCTGTTGAATTTGATGCGCGATCGATTATTCGAAAGCCCGTTTATCCACTGCGATGAAAC
ACGAGTCCAGGTACTAAAAGAGCCGGATCGTGACCCAACCAGCCAATCCTGGATGTGGGTGCAAGCCAGCGGACCGCCAGACCGAAAAGTCGTCCTGTTC
GATTACACCACCAGCCGTGCGCAGGAGGTGCCGCTGAGCCTGTTGGAAAGTTATCGCGGCTACGTGATGACCGATGATTACGCGGGCTATAACGCGTTGG
CGTCGCAACCGGGTGTGGAACGTCTGGCGTGCATGGCCCATGTACGACGCAAGTTCGTGGAGGCAAAGAAAGTGCAGCCCCAAGGCAAAACGGGCCGCGC
CGATGTTGCACTGGCATCTATCAATAAACTGTACGGCATCGAGCGCGAACTCAAGGATGTCAGCGATGAGCAGCGTTACGTCGGCCGTCAGGAAAAAAGC
CTGCTTGAGCTGGCGAAGCTGAAAGCATGGATGGAAAAAACGCAGCCGCAGGTCACCTCGCAAAGTGCGTTGGGCAAAGCGGTGAATTACCTGGCCAACA
ACTGGACCCGGCTGGAGCGTTATATCGAAGCTGGTTTTTTACCCATCGACAACAACGCTGCCGAACGAGCGATCAGACCGTTTGCCATCGGACGAAAAGC
CTGGCTGTTCAGCGACACACCCAAGGGCGCCACCGCCAGCGCCCAGATCTACAGCTTGGTCGAGACCGCCAAACTCAACGGCCAAGAGCCTTATACGTGG
CTGCGCCACGTACTGGAGCGGTTGCCGCATGCTGCATCGGTAGAAGACTACGAAGCCTTGTTGCCATGGAACTGCTCGCCAGAGATGCCTCGGTAAACCA
GATCCTCATTTTTTGCCAGGTGGGGTTTGTGGCGCGGATAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
360 bp	119 aa	87	446	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MMRPDAKVEKVYLYPKPVDFRKSIDGLAALVELDIKVAVFDPVLFVFLNRHRNRVKILYWERNGFCLWLKRLESERFKTSPDETDEAIVLTVQELNWLLD
GFDLWRNRPHKVLTPRFVA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1533 bp	510 aa	464	1996	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISMPEDLPDDPVLLKQLLAQLLIERTADKGHIVDLKEQIKLLRDRLFGRKSEQTVEPNTPQLALFNEPESEPMLPVGDAEEEVVAPVKRRGKRKPLSAD
LPRIEVIHELPEHELTCACGCRKHVISEETSEQLDIVPMQIRVIKHIRKVYGCRSCETAPVTADKPAQLIEKSMASPSVLAMLLTTKYVDGLPLHRFETV
LSRHGIEIPRQTLARWVIQCSEHFQPLLNLMRDRLFESPFIHCDETRVQVLKEPDRDPTSQSWMWVQASGPPDRKVVLFDYTTSRAQEVPLSLLESYRGY
VMTDDYAGYNALASQPGVERLACMAHVRRKFVEAKKVQPQGKTGRADVALASINKLYGIERELKDVSDEQRYVGRQEKSLLELAKLKAWMEKTQPQVTSQ
SALGKAVNYLANNWTRLERYIEAGFLPIDNNAAERAIRPFAIGRKAWLFSDTPKGATASAQIYSLVETAKLNGQEPYTWLRHVLERLPHAASVEDYEALL
PWNCSPEMPR

Blast result :

Comments

ISPsy34 is 97% aa (ORFA) 96% aa (ORFB : the transposase) and similar to IS684.

References

1] Margareth Butler (2015) Direct submission
2] McCann,H.C., Rikkerink,E.H., Bertels,F., Fiers,M., Lu,A., Rees-George,J., Andersen,M.T., Gleave,A.P., Haubold,B., Wohlers,M.W., Guttman,D.S., Wang,P.W., Straub,C., Vanneste,J.L., Rainey,P.B. and Templeton,M.D. (2013) PLoS Pathog. 9 (7), E1003503.