ISfinder Mirror Site

ISPsy32

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP011972	ND	Pseudomonas syringae	Pseudomonas syringae pv. actinidiae ICMP 18884

DNA section

IS Length : 1170 bp

Ends

IR Length : 23/27

IRL : GTGTACTGTCTCAAAAATAAATGCTCTGTAAAATCGTCTATGCTTGCTCG
IRR : GTGTACCGTCTCAAAATTAACTTCTCTCATGCCTGCGTGCTCATTGCCAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATTTCGAAAACAGCACAC	TA	GATTATTTCTACTCAGCG	2
TACGGGCCCGGTTGGACC	TA	GCGGACTTTTGCGGGCTT	2
AAAATTTGAATTTTCCGC	TA	GCCAGTAGCTGGTTTATT	2
CAAAGACCTCAGTCTAAC	TA	GCTCCGGTTTAAAAATAC	2
CCACATGCAGACATCAAC	TA	GCGTTCCTCTCTCGGTTC	2
TCTTCGGGTACAAAGATC	TA	GTGTGCTGTTTTCGAAAT	2
CCAGACGCTTGCTGCCTC	TA	GTTACCACTTTGCTGACC	2
AATTTTGAGACGGTACAC	TA	GCGCGTCACTCCCACCCA	2
CTGATTCAGAAGATCTGC	TA	GCAGTTGCCTCACCAAAC	2
ATCTTGGGTGCCAGCCTAGC		TAGAAAGTACACAGAGTGTA	0
ATTTCGCGAACAGCACAC	TA	GTACCTTTCAGACAAACC	2
ATTTCGAAAACAGCACAC	TA	GATTGCCATCAAAAATAG	2
TGCGTGCTCCAGGTCAGC	TA	GAGATCCATAAACGAGAA	2
CTACCGTCTGCTAGTCTC	TA	GTGTGCTGTTTTCGAAAT	2
TGATTAGTGGCTCTATGC	TA	GCCAGATGGCGAGATTAA	2
GTATTTTCCTTGCTCAAC	TA	GCAGAAGTGATAGGTATC	2
AATTTTGAGACGGTACAC	TA	GCACCGAGACTGGGGTCG	2
TGGCAAAACGCAACGCGC	TA	GTCCGTATTGCTGGAAAC	2
CACCGCCACGCAGGCTGC	TA	GTGTACTGTCTTAAAAAT	2
AATTTTGAGACGGTACAC	TA	GGTTCAGCGGTAAAGTAA	2
ATCGAAGAACGTGACACC	TA	GGTCAACTGCCGAGCGAA	2
CACGCTAAGTTCATCGCC	TA	GCTCCGAAAGCTCGTCTG	2
CACGCGAACTTGAGACGC	TA	GCTGAATACCAGAAGCTG	2
ATTTATTAGATCGGTTGC	TA	TGAAAGCGTCATAGCAAT	2
CTCGAAATCAACGAATAC	TA	GTGTACTGTCTCAAAAAT	2
CTACACCTAACGCCAACC	TA	GAACTGACCCTCTGGGTA	2
GAAGCAACTGTCGTATCC	TA	GTGATCCACTTTGCCCAT	2
AGTTAATACTCGAGGCGC	TA	GGCTTGTCGATACGTCTA	2
CCATACTTCACTCGCTAC	TA	GACGGTTTGATCGATAAG	2
TAGCAAAAAAGACGACCC	TA	GTGAAATCTGGATAAATC	2
CCTGATCTACCAGAGCGC	TA	GGGTATAGCTGAGTATTG	2
AGAGAAACGTACGAAGAC	TA	GAAGAGCGGGGTATTACT	2
CTTGCGCGGTGGTGTCTC	TA	GCGAAATACTCTACGACC	2
GATTTTTTCCGAATTCTC	TA	GTGTGCTGTTTTCGAAAT	2
GAATATTCCTACAGCAAC	TA	GGGTGGCGTCCTATAGCT	2
GTCATATCGCTTAGCACC	TA	GCCCGTTTGCAGCGGCGC	2
GTGTTGATAATTAGCATC	TA	GAAACGCGAGGTTCGGCT	2
AGAGCTGATAAGGGAACC	TA	GAATTGTTGGTGCCATCA	2
GACTTGCAAAATCGACGC	TA	GCGTAGTGGTCGATGATG	2
CAGAATACCCAGCGCAGC	TA	GACCATGGGAGACTTTGC	2
ATCAACAAAAAACCACTC	TA	GGCCGCATCATTGACGTC	2
ATCAGGCGTGGCGATGC	TAA	AGAAAATGGCCTACAAG	3
AGACAAGCAGCAGGCTAC	TA	GCATCGTTCAACGTGGCA	2
CACACCAAAAATTTCATC	TA	GCGAATTCAACTCGCGGC	2
ACCTACGTGCCAAATCAC	TA	GGGCATAAGGAAGTACGC	2
TATTCAAAAACAGTACAC	TA	GTAGATTGAGAGGTAAAT	2
TGCACTCATCGAGCACAC	TA	GAGGGTCGTGACAAAATT	2
TCACCTCTTCTGCAAGAC	TA	GCACCCCTGCGACGAGAC	2
CAGCGAGCAAAGGGTATC	TA	GTGTACTGTTTTTGAATA	2
TAGAGTTTTCCGTCTAGC	TA	GATAAAAATGAGTAAGGC	2
ATCATAGAATGGATTTGC	TA	GCGGCGTTTTTCAAGGTA	2
TACCTTCTTCAAATTATC	TA	GTGTACTGTCTCAAAAAT	2
ATTCTCATTTATTGCTCC	TA	GTTGGATGGAAATATCTT	2
ATTTACATTCTGTGCCAC	TA	GGCCCCCAATCAATTCTG	2
CCTGCCACCTGGACCACC	TA	GAGAATTAAAGAATGGAT	2
ATTTCGAAAACAGCACAC	TA	GATTAATACGAGAGTTTG	2
TCGTATAGGAAAACGATC	TA	GGAGGTGAAAATGGAGTT	2
TCGGCATACATGCACCTC	TA	GATTAATACGAGAGTTTG	2

DNA sequence

GTGTACTGTCTCAAAAATAAATGCTCTGTAAAATCGTCTATGCTTGCTCGTTGATTACACCGTTGGAGGCAAAGAAATGAGCGCCCAAGATATCGTCCTG
AGTCCTGAGGAAAGCGCCGAGCTGAGCCGTCGCACACGATCAGCCACCATCAGTCAGCGTGACGGTCGCCGCGCGAGGGTCATCCTGTTAGCTGCTCAAG
GTTGTTCAAGGGTTGAAATAGCCCGCCTGGTTGGATTCTCTCTTCGCTCAGTCACTCTTTGGTGCAAGCGTTTTCAAGAACAGGGACTAGACGGTTTGAT
CGATAAGCCTGGCCGCGGACGTAAGTCATTCTTGCCGCCAGACGCTATGGCTCGAGTGCTTGAAAAAGTGGTTCAACCTCGTATTGGCCAGCCTCGCTGG
AGCTGCCGAAGCATGGCGCAAGTCGCGGGTATTTCTCCGGCCAGCGTCCAGCGTATATGGGCTGCCAATGACATAAAGCCGCACCTGACTCGTACGTTCA
AGCTGTCCAACGATCCCAACTTCGAAGAGAAATTCTGGGACGTTATCGGGTTGTATCTGGACCCGCCAGACAAGGCACTGGTGCTGTGCTGCGATGAAAA
AAGTCAGGTTCAGGCCTTGGAGCGTACCCAGCCGGGACTGCCTCTGGGCATCGGCCATATCCGCACGCAATCGCACGATTACATTCGCCATGGCACCGTG
ACCCTGTTCACTGCGCTCGATTACCTTGAGGGGAAGTTGATCAGTTCTATCGAGCGCCAACACCGGCATCAGGAGTGGCTGGACTTCCTCAAGAAAATCA
ATCGGGAAACGCCCAAGCACCTTCAATTGCACCTGATCGTCGACAACTACGCCACGCACAAACATCCGAAAGTGAAGGCCTGGCTCGAAAAGCACAAGCG
CTTCCATATGCATTTCACCCCGACCTCAAGTTCGTGGATGAACATGGTCGAGCGCTTCTTTCGTGACATCACGGTATACCTGCGCGACGGTAGTTTCAGC
TCGATTCGCGAATTGAAAAGCTCAATCACCACATTTCTGGCACTGCGAAATGCCCAGCCGACTCGCTACGTTTGGAACGCTAAGGGAGAAGATATTTTGA
ACAAAATCCAGCGAGCCCGCGTGGCAATGAGCACGCAGGCATGAGAGAAGTTAATTTTGAGACGGTACAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1068 bp	355 aa	77	1144	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSAQDIVLSPEESAELSRRTRSATISQRDGRRARVILLAAQGCSRVEIARLVGFSLRSVTLWCKRFQEQGLDGLIDKPGRGRKSFLPPDAMARVLEKVVQ
PRIGQPRWSCRSMAQVAGISPASVQRIWAANDIKPHLTRTFKLSNDPNFEEKFWDVIGLYLDPPDKALVLCCDEKSQVQALERTQPGLPLGIGHIRTQSH
DYIRHGTVTLFTALDYLEGKLISSIERQHRHQEWLDFLKKINRETPKHLQLHLIVDNYATHKHPKVKAWLEKHKRFHMHFTPTSSSWMNMVERFFRDITV
YLRDGSFSSIRELKSSITTFLALRNAQPTRYVWNAKGEDILNKIQRARVAMSTQA

Blast result :

Comments

ISPsy32 is 93% aa similar to ISPpu1.

References

1] Margareth Butler (2015) Direct submission
2] McCann,H.C., Rikkerink,E.H., Bertels,F., Fiers,M., Lu,A., Rees-George,J., Andersen,M.T., Gleave,A.P., Haubold,B., Wohlers,M.W., Guttman,D.S., Wang,P.W., Straub,C., Vanneste,J.L., Rainey,P.B. and Templeton,M.D. (2013) PLoS Pathog. 9 (7), E1003503.