ISAba32

  • Family ISNCY
  • Group IS1202
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii isolate D36
DNA section
IS Length : 1482 bp

Ends


IR Length : 20/26

IRL : TGTTAGAACCCATTTAAAGTGTCTATATTCTCACCAATAAAAATGTCTGG
IRR : TGTTATTCACCAATTAAAATGTCTATGCGAAAAACCATTTAAAATGTCCT

Insertion site


Left flankDirect repeatRight flankDR Length
TTAATATATAAGTGTACTTCGTATA5

DNA sequence

TGTTAGAACCCATTTAAAGTGTCTATATTCTCACCAATAAAAATGTCTGGTTTATGATGGTTTTTATCACCATGGACTGGATATAAAATATAGATGCTGA
TCACTATGTCTGACAAAGAAATTCAACGTCTTGCAGTTCTGCAAGACGTTCGAGATCATCGTATTACCCAAGTCCGTGCTGCTGAAATCCTGAATCTTTC
CACCCGTCAAATTACCCGGTTATTGCAGAAGCTCAATCAAGATGGTGTTTCAGGCATGACGCATGCCAGTCGTGGTCAACCTGGCCATCGTCGCCATGAT
GACTTGTTAAAATCAAAGTGCCTTTCCATTATTGCTGAACATTTGCTGGGCTTTGGACCCACCTTGGCGCATGAGAAGCTCAGCAGCATGTTTGGTCTGC
ATATCCCTGTAGAAACGGTTCGTCGCTGGATGACTGCAAATGACCTGTGGATTCCTCGATCCAAGCGTCTGAAACGTCCGTATCAGCCTAGATATAACCG
TGATTGCTTCGGTGAGCTGATCCAGATCGATGGCTCATATCATGACTGGTTTGAAGGCCGAGCTTCCAAATGCTGCTTGCTGGTTTATATCGATGACGCC
ACTGGAAAGCTGTTGCATCTGCGCTTTTGTGAGGCTGAAACGACCTTTGATTATATGCTTTCAACCCGAGCCTACATTGAGCAATACGGCAAGCCTTTAG
CGTTTTATAGCGATAAACACTCGGTATTCCGAGTGAATCAGAAATCGAGCCAAGATAGCCAGATCACGCAATTTGGGCGGATTCTGAATGAGTTAAATAT
TGATATTATCTTCGCCAACTCACCCCAGGCTAAAGGCCGTGTGGAACGTGCCAATAGAACACTCCAGGATCGCCTGATCAAGGAAATGCGCCTAGAAGGT
ATCTGTTCAATTGCCGAGGCAAATGCCTGGCTGCCCTGCTTTATTGAGCATTTCAATCAGAAGTTTGCCAAGTGTGCGCGAAACTCAAAGAATTTACATC
GGCCATTAACCGAATCTCATCTTGAACTGGATGATATTTTTACCTGGCAGGAACCGCGTAAGGTCACCAAGAATCTGACCCTGACCTATGACAAATGTAT
TTATCTGCTTGAACCGATAGAGCTGAATCATAAGCTTGTTGGGCAATATATTTCATTTCTGGAGTACCCGGATGGGACTGTGGCCCTCATGCACGAGGGA
CGAAAGCTCAACTACAGCATTTTCAATAAATTAGCTGGGCTACAGCAGAATGAGATCGTTGAGAATAAACGGTTAGGTACAGTTCTGGCACATATTCAGC
AACAGCATGAAGAGTTGGAAAAACAGAATAAACGTTCCCGTCTCAAGAAAAGTATGCCGAGCCGTAAAGCTCAGAAAGCTGTTATTGAACAAAGAAATTT
AAATCCTGTGCTTGACTCTTGTGGTTAAAAACAGGACATTTTAAATGGTTTTTCGCATAGACATTTTAATTGGTGAATAACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1338 bp445 aa941431+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLITMSDKEIQRLAVLQDVRDHRITQVRAAEILNLSTRQITRLLQKLNQDGVSGMTHASRGQPGHRRHDDLLKSKCLSIIAEHLLGFGPTLAHEKLSSMF
GLHIPVETVRRWMTANDLWIPRSKRLKRPYQPRYNRDCFGELIQIDGSYHDWFEGRASKCCLLVYIDDATGKLLHLRFCEAETTFDYMLSTRAYIEQYGK
PLAFYSDKHSVFRVNQKSSQDSQITQFGRILNELNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIAEANAWLPCFIEHFNQKFAKCARNSKN
LHRPLTESHLELDDIFTWQEPRKVTKNLTLTYDKCIYLLEPIELNHKLVGQYISFLEYPDGTVALMHEGRKLNYSIFNKLAGLQQNEIVENKRLGTVLAH
IQQQHEELEKQNKRSRLKKSMPSRKAQKAVIEQRNLNPVLDSCG

 

Blast result :
Comments
ISAba32 is 71% aa identical to the tnp of ISAjo2. The isolate D36 is recovered from a wound sample in a Sydney hospital in 2008 .
References
1] Hamidian M., Hall R.M (2015) Direct submission