ISAhy3

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP006870 ND Aeromonas hydrophila
Aeromonas hydrophila NJ-35
DNA section
IS Length : 1221 bp

Ends


IR Length : 24/26

IRL : TGTAGATTCAATTGGTCAACGCAACAACCATGTGAAAACATGCAGTTGCG
IRR : TGTAGATTCAACTTGTCAACGCAACACTCTTTTCAATAACTTCTTTTGGC

Insertion site


Left flankDirect repeatRight flankDR Length
AACAGGCTGTATTACCTATT0

DNA sequence

TGTAGATTCAATTGGTCAACGCAACAACCATGTGAAAACATGCAGTTGCGGAGGATTGTTGAATGAAACGAACATTTACAGCAAAGGAAAAAGCGTTTGT
TTTTGATCTGTGGAAGCGCGGTACTGGCTTCAGTGATATAGCAAAAATCCTGAATTCAAAACCCGGTACAATTTTCACCATGTTGAGAGATACGGGAGGC
ATCAAACCGACCGAGCGTCGCCGAGCAGTAGCTCACCTGACGCTATCCGAACGTGAGGAGATTCGAGCCGGTCTTTCTGCAAAGATGAGTATCCGTGATA
TAGCGCGGGCCCTTAACCGCTCTCCATCAACAATATCTCGGGAAGTTCAACGCAACCGTGGCAGACGCTATTACAAAGCTGTTGATGCTAATCATCGCGC
TAACCGTATGGCTAAAAGACCCAAAGCATGCCTTCTCGAACGGAATTCGCGACTGCGAGAACTGGTGCTGGAAAAGCTCGAGTTGAAATGGTCTCCAGAG
CAGATATCGGGGTGGCTCAAAAGAACGATGCCTCGCCAGAAAGCGATGCAGATCTCTGCCGAAACGATCTATAAAACGTTGTATTTTCGTAGCCGGAGTG
CGCTGCACCATCTGCTTGTCAAGCATCTACGTCGTTCTCACAGCCTGCGTCATGGGAAACGCCATACCCGCAAGGGAGAGAGGGGCACCATCAATATAGT
GAACGGTATTTCAATCCATGAGCGCTCCAGACACATCGATAATCGACGAACTTTTGGTCACTGGGAAGGCGACTTGGTTTCCGGCACTAACAACTCCCAC
ATTGCCACGCTAGTAGATAGAAAGTCGCGCTACACCATCATTCTCAAGCTAAGTGGAAAAGATGCTGGTTCTGTGAATCAAGCAATTACAGAAAAATTTA
AGACGTTGCCGCGTAAGCTCAGACAGTCGCTGACATGGGATCGCGGCATGGAACTGGCCAAGCATCTGGAGTTTACTGCAAACACTGGGGTGAAGGTTTA
CTTCTGTGATCCCCAGAGTCCCTGGCAACGGGGGACGAATGAAAACACCAATGGGCTGATCCGACAATACTTCCCCAAAAAGACATGTCTTGCCCAATAT
TCCCAGCAAGAACTGGATAAGGTTGCAGCCCAACTAAACAGCAGGCCGAGGAAGACCCTGAAATTTAAAACGCCAAAAGAAGTTATTGAAAAGAGTGTTG
CGTTGACAAGTTGAATCTACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1152 bp383 aa631214+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRTFTAKEKAFVFDLWKRGTGFSDIAKILNSKPGTIFTMLRDTGGIKPTERRRAVAHLTLSEREEIRAGLSAKMSIRDIARALNRSPSTISREVQRNRG
RRYYKAVDANHRANRMAKRPKACLLERNSRLRELVLEKLELKWSPEQISGWLKRTMPRQKAMQISAETIYKTLYFRSRSALHHLLVKHLRRSHSLRHGKR
HTRKGERGTINIVNGISIHERSRHIDNRRTFGHWEGDLVSGTNNSHIATLVDRKSRYTIILKLSGKDAGSVNQAITEKFKTLPRKLRQSLTWDRGMELAK
HLEFTANTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYSQQELDKVAAQLNSRPRKTLKFKTPKEVIEKSVALTS

 

Blast result :
Comments
ISAhy3 is 92% aa similar to IS30.
References
1] Pfeiffer F. (2015) Direct submission
2] Pang,M., Jiang,J., Xie,X., Wu,Y., Dong,Y., Kwok,A.H., Zhang,W., Yao,H., Lu,C., Leung,F.C. and Liu,Y. (2015) Sci Rep 5, 9833