ISAve4
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002607 | ND | Aeromonas veronii | Aeromonas veronii B565 |
DNA section
IS Length : 1239 bp
Ends
IR Length : 26/35
IRL : TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTACAGTCGCCAC
IRR : TGTAGTGGTCAACTAAATCCGGACACTTGGTTAGGCCGTTCTTCGGCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTAGGTATC | TCC | CTTTAAAAAA | 3 |
| TGCGTCGAAC | GACTTTAGCC | 0 | |
| ATCGACAACC | ATGTCGCCCC | 0 |
DNA sequence
TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTACAGTCGCCACCATGACCTGAGGTGTTCAATGACAAGATTACGTCGCTCATTTACTGCCCA
ATTCAAACTTGAAGCCGCATCCCTGGTGCTCGACCAGGGATATTCTGTCCCTGAAGCCAGTCGTTCACTGGATGTCGGTGAAACCGTACTTCGCCGCTGG
GTTCAGCAACTGCAATCCGAACGCAGTGGCACAACCCCAACTAATAAAGCGCTGACACCAGAACAGCAGAAAATTCAGGAGCTGGAAGCCCGCATCAACC
GGCTCGAACGTGAAAAGGACATTCTAAAAAAGGCTACTGCTCTCTTGATGGCGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACTCTGGTCTGCTGCGCCTTCGATGTTCCTACCTCCTGTTTTTACGATTATCTGGCTCGCAGGCGGACGACTAACCGTGAACGTATGCAACAGC
GCTGTGAGTTGCGCCGTTTGTTCAAAGAAAGCCGGAACTCAGCGGGCAGCCGTGCCTTGATGTCGATGATGCGAGAGTTGGGGTATCAGATTGGTCGATG
CAAAGTACGTAACCTGATGAAGGAAGCCGCTCTGGTATCCAAACAGCCGGGCGCGCACCGCTATAAGGTGGCGCGGTCAGAACGACCGGATATACCCAAT
CTGCTGGCCCGAGAATTTGATGTCCAGCAACCCAATCAGGTGTGGTGTGGCGATATCACCTACGTCTGGGCAGGTGGTCGCTGGCATTACCTGGCAGCGG
TTCTTGACCTGCACACCCGGCGGGTCGTGGGCTGGGCGATGTCAGATAAACCAGATGCCGAGCTGGCCATCAAAGCACTTGAGATGGCGTATCAGCAACG
AGGTTGTCCGTCAGGCGTGCTGTTTCACTCTGACCAGGGCAGCCAATATGGCAGCCGGGCATTTCGGCAACGACTGTGGCGCTACCGCATGACCCAGAGT
ATGAGTCGGCGTGGCAACTGCTGGGATAACGCCCCGATGGAACGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTCCCGGCTATGGGATATATGAGCTTGC
GAGAGGCAAAACGAGATATCAGTTATTACCTGATGGATTACTACAGCTGGCGGCGTCCACATCAACACAATGACGGGATACCGCCAGCAAAAGCCGAAGA
ACGGCCTAACCAAGTGTCCGGATTTAGTTGACCACTACA
ATTCAAACTTGAAGCCGCATCCCTGGTGCTCGACCAGGGATATTCTGTCCCTGAAGCCAGTCGTTCACTGGATGTCGGTGAAACCGTACTTCGCCGCTGG
GTTCAGCAACTGCAATCCGAACGCAGTGGCACAACCCCAACTAATAAAGCGCTGACACCAGAACAGCAGAAAATTCAGGAGCTGGAAGCCCGCATCAACC
GGCTCGAACGTGAAAAGGACATTCTAAAAAAGGCTACTGCTCTCTTGATGGCGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACTCTGGTCTGCTGCGCCTTCGATGTTCCTACCTCCTGTTTTTACGATTATCTGGCTCGCAGGCGGACGACTAACCGTGAACGTATGCAACAGC
GCTGTGAGTTGCGCCGTTTGTTCAAAGAAAGCCGGAACTCAGCGGGCAGCCGTGCCTTGATGTCGATGATGCGAGAGTTGGGGTATCAGATTGGTCGATG
CAAAGTACGTAACCTGATGAAGGAAGCCGCTCTGGTATCCAAACAGCCGGGCGCGCACCGCTATAAGGTGGCGCGGTCAGAACGACCGGATATACCCAAT
CTGCTGGCCCGAGAATTTGATGTCCAGCAACCCAATCAGGTGTGGTGTGGCGATATCACCTACGTCTGGGCAGGTGGTCGCTGGCATTACCTGGCAGCGG
TTCTTGACCTGCACACCCGGCGGGTCGTGGGCTGGGCGATGTCAGATAAACCAGATGCCGAGCTGGCCATCAAAGCACTTGAGATGGCGTATCAGCAACG
AGGTTGTCCGTCAGGCGTGCTGTTTCACTCTGACCAGGGCAGCCAATATGGCAGCCGGGCATTTCGGCAACGACTGTGGCGCTACCGCATGACCCAGAGT
ATGAGTCGGCGTGGCAACTGCTGGGATAACGCCCCGATGGAACGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTCCCGGCTATGGGATATATGAGCTTGC
GAGAGGCAAAACGAGATATCAGTTATTACCTGATGGATTACTACAGCTGGCGGCGTCCACATCAACACAATGACGGGATACCGCCAGCAAAAGCCGAAGA
ACGGCCTAACCAAGTGTCCGGATTTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 69 | 377 | + | No |
Description : First part of the transposase
ORF sequence :
MTRLRRSFTAQFKLEAASLVLDQGYSVPEASRSLDVGETVLRRWVQQLQSERSGTTPTNKALTPEQQKIQELEARINRLEREKDILKKATALLMADEFTR
TR
TR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 314 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
KGHSKKGYCSLDGGRVHTYALIDRLREQAPITLVCCAFDVPTSCFYDYLARRRTTNRERMQQRCELRRLFKESRNSAGSRALMSMMRELGYQIGRCKVRN
LMKEAALVSKQPGAHRYKVARSERPDIPNLLAREFDVQQPNQVWCGDITYVWAGGRWHYLAAVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPS
GVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPAMGYMSLREAKRDISYYLMDYYSWRRPHQHNDGIPPAKAEERPNQ
VSGFS
LMKEAALVSKQPGAHRYKVARSERPDIPNLLAREFDVQQPNQVWCGDITYVWAGGRWHYLAAVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPS
GVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPAMGYMSLREAKRDISYYLMDYYSWRRPHQHNDGIPPAKAEERPNQ
VSGFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 69 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRLRRSFTAQFKLEAASLVLDQGYSVPEASRSLDVGETVLRRWVQQLQSERSGTTPTNKALTPEQQKIQELEARINRLEREKDILKKGYCSLDGGRVHT
YALIDRLREQAPITLVCCAFDVPTSCFYDYLARRRTTNRERMQQRCELRRLFKESRNSAGSRALMSMMRELGYQIGRCKVRNLMKEAALVSKQPGAHRYK
VARSERPDIPNLLAREFDVQQPNQVWCGDITYVWAGGRWHYLAAVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPAMGYMSLREAKRDISYYLMDYYSWRRPHQHNDGIPPAKAEERPNQVSGFS
YALIDRLREQAPITLVCCAFDVPTSCFYDYLARRRTTNRERMQQRCELRRLFKESRNSAGSRALMSMMRELGYQIGRCKVRNLMKEAALVSKQPGAHRYK
VARSERPDIPNLLAREFDVQQPNQVWCGDITYVWAGGRWHYLAAVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPAMGYMSLREAKRDISYYLMDYYSWRRPHQHNDGIPPAKAEERPNQVSGFS
Blast result :
Comments
ISAve4 is 96% aa similar to ISAs6.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390
2] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390