ISAcsp5

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
APOK01000044 ND Acinetobacter sp.
Acinetobacter venetianus strain VE-C3 plasmid pAV2
Acinetobacter sp. CIP A165
Acinetobacter baumannii BM2686 plasmid pIP1858
DNA section
IS Length : 1288 bp

Ends


IR Length : 20/27

IRL : TGATGTGGTTCTAGGATTTTAGACCACGCCTATAAGTGATAATCTACTCC
IRR : TAATGTGGTTCCGTTAATTAGACCACTCAATTCAGTTTTATAAGTGATAA

Insertion site


Left flankDirect repeatRight flankDR Length
TAGCTTCTGTTGATCACATTGATA4
CTATTCAATTGTTGCTTTAT0

DNA sequence

TGATGTGGTTCTAGGATTTTAGACCACGCCTATAAGTGATAATCTACTCCCCAATAAGCATGGGAAATGCTTGTTTTTGGAGTCAACCATGACACGAAGA
AAACGTCGTAATCATTCAGCAGAGTTTAAAGTTAAAGTTGCTCTCGCAGCAATTAAAGGCGACCACACACTCGCTGAACTTTCTACTCAATTCGATTTAC
ATCAAAACCAAATCATCGATTGGAAAAATCAACTGCTTGAGCAATCAGTCAATATTTTTTCACGACCAACAGCACAACAAGAACCAGAGATTGACCTCAA
AGCTCTACATGCCAAGATAGGACATCAGGCATTGCAAATTGATTTTTTAGAAGGTGCGCTCAGAAAAATAGGGCAGCTGAGCGGCAAAAAATGATCGATA
AGACCCATCAACTTTCGGTACGACAACAATCGCAATTGATTCAAATCAATCGCAGTACGTTGTATTACAAGTCCAAAGAGATTTCATCAACTGATTTGAG
TTTGATGCGCCTGATCGATGAAATTCATCTCGACTACCCATTTATGGGCAGTCGAATGATACGAGATATGCTACAGCGTCAAGGGCATCAAATAGGTCGG
CGTAAAGTCCGACGTTTAATGCGCTTGATGGGAATACATGCCTTGTATCCAAAACCCAATACCAGTAAGCCTAATCTTGCACACCGTATTTTCCCATATC
TGTTGAAAAACATGGTCATCGATCACTCTAATCAAGTCTGGTGTACAGATATCACGTACATTCCTATGGCTAAAGGCTTTGTCTATCTGTGTGCAATTAT
AGATTGGCATAGTCGTAAAGTACTGGCTCATCGTGTATCGATCAGTATGGAAACAGGCTTTTGCATAGATGCGTTGCAAGAAGCAATTGTGAAATATGGT
TGTCCTGAGGTGTTTAATACAGACCAAGGCAGTCAATTCACAAGCGAGGCATTTTTGAATGAGTTAAAATTGCGAAATATCCGTATCAGTATGGATGGGA
AAGGACGATGGATGGATAATGTAATGATTGAGCGTTTATGGCGCAGCGTGAAGCATGAGGAAGTCTATTTGAAGGCTTACGATACGGTTAAACAAGCGAA
ACAATCAATTGCTGAATATTTGGATTTTTATAACATGATACGTCCTCATTCAAGTTTGAATAAGGCAACACCGGATGAATTTTATGATCAACATTTACTA
AAAGTAATGGCAGCATAATTTAAATATTTAGAGCGGATTTATCACTTATAAAACTGAATTGAGTGGTCTAATTAACGGAACCACATTA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
306 bp101 aa89394+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTRRKRRNHSAEFKVKVALAAIKGDHTLAELSTQFDLHQNQIIDWKNQLLEQSVNIFSRPTAQQEPEIDLKALHAKIGHQALQIDFLEGALRKIGQLSGK
K

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
876 bp291 aa3431218+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

FFRRCAQKNRAAERQKMIDKTHQLSVRQQSQLIQINRSTLYYKSKEISSTDLSLMRLIDEIHLDYPFMGSRMIRDMLQRQGHQIGRRKVRRLMRLMGIHA
LYPKPNTSKPNLAHRIFPYLLKNMVIDHSNQVWCTDITYIPMAKGFVYLCAIIDWHSRKVLAHRVSISMETGFCIDALQEAIVKYGCPEVFNTDQGSQFT
SEAFLNELKLRNIRISMDGKGRWMDNVMIERLWRSVKHEEVYLKAYDTVKQAKQSIAEYLDFYNMIRPHSSLNKATPDEFYDQHLLKVMAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1130 bp376 aa891218+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRRKRRNHSAEFKVKVALAAIKGDHTLAELSTQFDLHQNQIIDWKNQLLEQSVNIFSRPTAQQEPEIDLKALHAKIGHQALQIDFFRRCAQKNRAAERQ
KMIDKTHQLSVRQQSQLIQINRSTLYYKSKEISSTDLSLMRLIDEIHLDYPFMGSRMIRDMLQRQGHQIGRRKVRRLMRLMGIHALYPKPNTSKPNLAHR
IFPYLLKNMVIDHSNQVWCTDITYIPMAKGFVYLCAIIDWHSRKVLAHRVSISMETGFCIDALQEAIVKYGCPEVFNTDQGSQFTSEAFLNELKLRNIRI
SMDGKGRWMDNVMIERLWRSVKHEEVYLKAYDTVKQAKQSIAEYLDFYNMIRPHSSLNKATPDEFYDQHLLKVMAA

 

Blast result :
Comments
ISAcsp5 is 79% aa similar to ISAba14. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Eun-Jeong YOON and Patrice Courvalin (2015) Direct submission.