ISAcsp5
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| APOK01000044 | ND | Acinetobacter sp. | Acinetobacter venetianus strain VE-C3 plasmid pAV2 Acinetobacter sp. CIP A165 Acinetobacter baumannii BM2686 plasmid pIP1858 |
DNA section
IS Length : 1288 bp
Ends
IR Length : 20/27
IRL : TGATGTGGTTCTAGGATTTTAGACCACGCCTATAAGTGATAATCTACTCC
IRR : TAATGTGGTTCCGTTAATTAGACCACTCAATTCAGTTTTATAAGTGATAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGCTTCTGT | TGAT | CACATTGATA | 4 |
| CTATTCAATT | GTTGCTTTAT | 0 |
DNA sequence
TGATGTGGTTCTAGGATTTTAGACCACGCCTATAAGTGATAATCTACTCCCCAATAAGCATGGGAAATGCTTGTTTTTGGAGTCAACCATGACACGAAGA
AAACGTCGTAATCATTCAGCAGAGTTTAAAGTTAAAGTTGCTCTCGCAGCAATTAAAGGCGACCACACACTCGCTGAACTTTCTACTCAATTCGATTTAC
ATCAAAACCAAATCATCGATTGGAAAAATCAACTGCTTGAGCAATCAGTCAATATTTTTTCACGACCAACAGCACAACAAGAACCAGAGATTGACCTCAA
AGCTCTACATGCCAAGATAGGACATCAGGCATTGCAAATTGATTTTTTAGAAGGTGCGCTCAGAAAAATAGGGCAGCTGAGCGGCAAAAAATGATCGATA
AGACCCATCAACTTTCGGTACGACAACAATCGCAATTGATTCAAATCAATCGCAGTACGTTGTATTACAAGTCCAAAGAGATTTCATCAACTGATTTGAG
TTTGATGCGCCTGATCGATGAAATTCATCTCGACTACCCATTTATGGGCAGTCGAATGATACGAGATATGCTACAGCGTCAAGGGCATCAAATAGGTCGG
CGTAAAGTCCGACGTTTAATGCGCTTGATGGGAATACATGCCTTGTATCCAAAACCCAATACCAGTAAGCCTAATCTTGCACACCGTATTTTCCCATATC
TGTTGAAAAACATGGTCATCGATCACTCTAATCAAGTCTGGTGTACAGATATCACGTACATTCCTATGGCTAAAGGCTTTGTCTATCTGTGTGCAATTAT
AGATTGGCATAGTCGTAAAGTACTGGCTCATCGTGTATCGATCAGTATGGAAACAGGCTTTTGCATAGATGCGTTGCAAGAAGCAATTGTGAAATATGGT
TGTCCTGAGGTGTTTAATACAGACCAAGGCAGTCAATTCACAAGCGAGGCATTTTTGAATGAGTTAAAATTGCGAAATATCCGTATCAGTATGGATGGGA
AAGGACGATGGATGGATAATGTAATGATTGAGCGTTTATGGCGCAGCGTGAAGCATGAGGAAGTCTATTTGAAGGCTTACGATACGGTTAAACAAGCGAA
ACAATCAATTGCTGAATATTTGGATTTTTATAACATGATACGTCCTCATTCAAGTTTGAATAAGGCAACACCGGATGAATTTTATGATCAACATTTACTA
AAAGTAATGGCAGCATAATTTAAATATTTAGAGCGGATTTATCACTTATAAAACTGAATTGAGTGGTCTAATTAACGGAACCACATTA
AAACGTCGTAATCATTCAGCAGAGTTTAAAGTTAAAGTTGCTCTCGCAGCAATTAAAGGCGACCACACACTCGCTGAACTTTCTACTCAATTCGATTTAC
ATCAAAACCAAATCATCGATTGGAAAAATCAACTGCTTGAGCAATCAGTCAATATTTTTTCACGACCAACAGCACAACAAGAACCAGAGATTGACCTCAA
AGCTCTACATGCCAAGATAGGACATCAGGCATTGCAAATTGATTTTTTAGAAGGTGCGCTCAGAAAAATAGGGCAGCTGAGCGGCAAAAAATGATCGATA
AGACCCATCAACTTTCGGTACGACAACAATCGCAATTGATTCAAATCAATCGCAGTACGTTGTATTACAAGTCCAAAGAGATTTCATCAACTGATTTGAG
TTTGATGCGCCTGATCGATGAAATTCATCTCGACTACCCATTTATGGGCAGTCGAATGATACGAGATATGCTACAGCGTCAAGGGCATCAAATAGGTCGG
CGTAAAGTCCGACGTTTAATGCGCTTGATGGGAATACATGCCTTGTATCCAAAACCCAATACCAGTAAGCCTAATCTTGCACACCGTATTTTCCCATATC
TGTTGAAAAACATGGTCATCGATCACTCTAATCAAGTCTGGTGTACAGATATCACGTACATTCCTATGGCTAAAGGCTTTGTCTATCTGTGTGCAATTAT
AGATTGGCATAGTCGTAAAGTACTGGCTCATCGTGTATCGATCAGTATGGAAACAGGCTTTTGCATAGATGCGTTGCAAGAAGCAATTGTGAAATATGGT
TGTCCTGAGGTGTTTAATACAGACCAAGGCAGTCAATTCACAAGCGAGGCATTTTTGAATGAGTTAAAATTGCGAAATATCCGTATCAGTATGGATGGGA
AAGGACGATGGATGGATAATGTAATGATTGAGCGTTTATGGCGCAGCGTGAAGCATGAGGAAGTCTATTTGAAGGCTTACGATACGGTTAAACAAGCGAA
ACAATCAATTGCTGAATATTTGGATTTTTATAACATGATACGTCCTCATTCAAGTTTGAATAAGGCAACACCGGATGAATTTTATGATCAACATTTACTA
AAAGTAATGGCAGCATAATTTAAATATTTAGAGCGGATTTATCACTTATAAAACTGAATTGAGTGGTCTAATTAACGGAACCACATTA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 89 | 394 | + | No |
Description : First part of the transposase
ORF sequence :
MTRRKRRNHSAEFKVKVALAAIKGDHTLAELSTQFDLHQNQIIDWKNQLLEQSVNIFSRPTAQQEPEIDLKALHAKIGHQALQIDFLEGALRKIGQLSGK
K
K
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 291 aa | 343 | 1218 | + | No |
Description : Second part of the transposase
ORF sequence :
FFRRCAQKNRAAERQKMIDKTHQLSVRQQSQLIQINRSTLYYKSKEISSTDLSLMRLIDEIHLDYPFMGSRMIRDMLQRQGHQIGRRKVRRLMRLMGIHA
LYPKPNTSKPNLAHRIFPYLLKNMVIDHSNQVWCTDITYIPMAKGFVYLCAIIDWHSRKVLAHRVSISMETGFCIDALQEAIVKYGCPEVFNTDQGSQFT
SEAFLNELKLRNIRISMDGKGRWMDNVMIERLWRSVKHEEVYLKAYDTVKQAKQSIAEYLDFYNMIRPHSSLNKATPDEFYDQHLLKVMAA
LYPKPNTSKPNLAHRIFPYLLKNMVIDHSNQVWCTDITYIPMAKGFVYLCAIIDWHSRKVLAHRVSISMETGFCIDALQEAIVKYGCPEVFNTDQGSQFT
SEAFLNELKLRNIRISMDGKGRWMDNVMIERLWRSVKHEEVYLKAYDTVKQAKQSIAEYLDFYNMIRPHSSLNKATPDEFYDQHLLKVMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1130 bp | 376 aa | 89 | 1218 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRRKRRNHSAEFKVKVALAAIKGDHTLAELSTQFDLHQNQIIDWKNQLLEQSVNIFSRPTAQQEPEIDLKALHAKIGHQALQIDFFRRCAQKNRAAERQ
KMIDKTHQLSVRQQSQLIQINRSTLYYKSKEISSTDLSLMRLIDEIHLDYPFMGSRMIRDMLQRQGHQIGRRKVRRLMRLMGIHALYPKPNTSKPNLAHR
IFPYLLKNMVIDHSNQVWCTDITYIPMAKGFVYLCAIIDWHSRKVLAHRVSISMETGFCIDALQEAIVKYGCPEVFNTDQGSQFTSEAFLNELKLRNIRI
SMDGKGRWMDNVMIERLWRSVKHEEVYLKAYDTVKQAKQSIAEYLDFYNMIRPHSSLNKATPDEFYDQHLLKVMAA
KMIDKTHQLSVRQQSQLIQINRSTLYYKSKEISSTDLSLMRLIDEIHLDYPFMGSRMIRDMLQRQGHQIGRRKVRRLMRLMGIHALYPKPNTSKPNLAHR
IFPYLLKNMVIDHSNQVWCTDITYIPMAKGFVYLCAIIDWHSRKVLAHRVSISMETGFCIDALQEAIVKYGCPEVFNTDQGSQFTSEAFLNELKLRNIRI
SMDGKGRWMDNVMIERLWRSVKHEEVYLKAYDTVKQAKQSIAEYLDFYNMIRPHSSLNKATPDEFYDQHLLKVMAA
Blast result :
Comments
ISAcsp5 is 79% aa similar to ISAba14. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Eun-Jeong YOON and Patrice Courvalin (2015) Direct submission.