ISAve3
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002607 | ND | Aeromonas veronii | Aeromonas veronii B565 |
DNA section
IS Length : 1333 bp
Ends
IR Length : 30/41
IRL : TAGATTGGCCCCTTGAAGATCCAGACACTTCGCCCCTCTTAACTTAAGAG
IRR : TGGATTTGACCCCATAGATCCAGACACTTTTTCCCGCTTAGGGTTGTGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACTGAATTG | GTGTA | ATAGGCTTAT | 5 |
DNA sequence
TAGATTGGCCCCTTGAAGATCCAGACACTTCGCCCCTCTTAACTTAAGAGGTAGTCTTGGAACTATGTTCAGTACTAGTCGCAACGAGAAGATGGTCGAA
GTGTTAACAGGGGCTGAGCGCCGTCGACACCGGACTCCGCAGGAAATGATCTCCATCGTGCAGCAGACCTTTGAGCCCGGGATGACCGTCTCTCATGTCG
CTCGCTTGCACGACGTCAACGCCAACCAGCTTTTCAAATGGCGTAAGCAATTCCAGGAAGGGTCTCTCACGGCGATCACCGCCGGTGAGGATGTTGTTCC
AGCCTCCGAGTTGGCTGCTGCCATCAAGCAGATCCGAGACCTCCAGCGTCTGCTAGGGAAGAAGACCATGGAGAACGAGATCCTCAGAGACGCTGTGGAG
TATGGTCGAGCAAAAAAATGGATTGCGCATGCGCCCTTGTTGCCGGGGGACGACGATTAAGTGCTGTGAGCCAGGCCCTTAATGTATCGCGTGCGCAGCT
ATCCGTTCGTGTTCATCGAAAACCAGGTTGGCAAGATGGTCGTCATCATCGCCTGTATGCTGATGCTGCCTTACTTGGCCGGATCATGGAGGCGATGGCT
GAGCTTCCCTCCTATGGCTATCGTCGAGTCTGGGCGCTGTTACGGCGTCAGTCAGAGGCAACACGACAGCCTGTGGTAAACGCCAAACGGGTATATCGCG
TCATGCGCGATCATGGACTGCTGCTGGAAAGAAAACCGGCTGCACCATTGACCCAGCGGGCACATAAGGGGCGTGTTGCCGTCAAGGAGAGCAATCGACG
TTGGTGCTCAGATGGCTTCGAGTTTCGCTGCGATAACGGTGAAAAACTACGAGTCACGTTTGCCATGGACTGCAGTGACAGGGAAACCTTGGACTGGGCT
GCCAGTACCGGGAGTTACGACAGAGCCACGGTGCAAGATGTCATGCTGAGGTCTGTTGAGCGTCGCTTCGGGGATGCGTTGCCGGCATCACCGGTGGAGT
GGCTGACTGATAATGGCTCGGCGTATCGAGCTCATGAGACCCGCGCGTTTGCACGAGAGCTCTGGTTGGAGCCCAGAACGACTGCGGTGAGAAGCCCACA
GAGCAACGGTATAGCGGAGAGCTTCGTGAAGACGATGAAGCGAGACTATATCGAGATGATGCCAAAACCGGATAGCCGAACAGCGGTAGGCAATTTTGCT
ATCGCGTTTGAGTATTATAACGAGCACCACCCGCACAGCGCCTTGGGATACCATTCACCCCGGGAATATCTGCGCAGTCGGGTATCACAACCCTAAGCGG
GAAAAAGTGTCTGGATCTATGGGGTCAAATCCA
GTGTTAACAGGGGCTGAGCGCCGTCGACACCGGACTCCGCAGGAAATGATCTCCATCGTGCAGCAGACCTTTGAGCCCGGGATGACCGTCTCTCATGTCG
CTCGCTTGCACGACGTCAACGCCAACCAGCTTTTCAAATGGCGTAAGCAATTCCAGGAAGGGTCTCTCACGGCGATCACCGCCGGTGAGGATGTTGTTCC
AGCCTCCGAGTTGGCTGCTGCCATCAAGCAGATCCGAGACCTCCAGCGTCTGCTAGGGAAGAAGACCATGGAGAACGAGATCCTCAGAGACGCTGTGGAG
TATGGTCGAGCAAAAAAATGGATTGCGCATGCGCCCTTGTTGCCGGGGGACGACGATTAAGTGCTGTGAGCCAGGCCCTTAATGTATCGCGTGCGCAGCT
ATCCGTTCGTGTTCATCGAAAACCAGGTTGGCAAGATGGTCGTCATCATCGCCTGTATGCTGATGCTGCCTTACTTGGCCGGATCATGGAGGCGATGGCT
GAGCTTCCCTCCTATGGCTATCGTCGAGTCTGGGCGCTGTTACGGCGTCAGTCAGAGGCAACACGACAGCCTGTGGTAAACGCCAAACGGGTATATCGCG
TCATGCGCGATCATGGACTGCTGCTGGAAAGAAAACCGGCTGCACCATTGACCCAGCGGGCACATAAGGGGCGTGTTGCCGTCAAGGAGAGCAATCGACG
TTGGTGCTCAGATGGCTTCGAGTTTCGCTGCGATAACGGTGAAAAACTACGAGTCACGTTTGCCATGGACTGCAGTGACAGGGAAACCTTGGACTGGGCT
GCCAGTACCGGGAGTTACGACAGAGCCACGGTGCAAGATGTCATGCTGAGGTCTGTTGAGCGTCGCTTCGGGGATGCGTTGCCGGCATCACCGGTGGAGT
GGCTGACTGATAATGGCTCGGCGTATCGAGCTCATGAGACCCGCGCGTTTGCACGAGAGCTCTGGTTGGAGCCCAGAACGACTGCGGTGAGAAGCCCACA
GAGCAACGGTATAGCGGAGAGCTTCGTGAAGACGATGAAGCGAGACTATATCGAGATGATGCCAAAACCGGATAGCCGAACAGCGGTAGGCAATTTTGCT
ATCGCGTTTGAGTATTATAACGAGCACCACCCGCACAGCGCCTTGGGATACCATTCACCCCGGGAATATCTGCGCAGTCGGGTATCACAACCCTAAGCGG
GAAAAAGTGTCTGGATCTATGGGGTCAAATCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 92 | 460 | + | No |
Description : First part of the transposase
ORF sequence :
MVEVLTGAERRRHRTPQEMISIVQQTFEPGMTVSHVARLHDVNANQLFKWRKQFQEGSLTAITAGEDVVPASELAAAIKQIRDLQRLLGKKTMENEILRD
AVEYGRAKKWIAHAPLLPGDDD
AVEYGRAKKWIAHAPLLPGDDD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1008 bp | 335 aa | 289 | 1296 | + | No |
Description : Second part of the transposase
ORF sequence :
GCCSSLRVGCCHQADPRPPASAREEDHGERDPQRRCGVWSSKKMDCACALVAGGRRLSAVSQALNVSRAQLSVRVHRKPGWQDGRHHRLYADAALLGRIM
EAMAELPSYGYRRVWALLRRQSEATRQPVVNAKRVYRVMRDHGLLLERKPAAPLTQRAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCSDRET
LDWAASTGSYDRATVQDVMLRSVERRFGDALPASPVEWLTDNGSAYRAHETRAFARELWLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAV
GNFAIAFEYYNEHHPHSALGYHSPREYLRSRVSQP
EAMAELPSYGYRRVWALLRRQSEATRQPVVNAKRVYRVMRDHGLLLERKPAAPLTQRAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCSDRET
LDWAASTGSYDRATVQDVMLRSVERRFGDALPASPVEWLTDNGSAYRAHETRAFARELWLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAV
GNFAIAFEYYNEHHPHSALGYHSPREYLRSRVSQP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 401 aa | 92 | 1296 | + | Yes |
Chemistry : DDE
ORF sequence :
MVEVLTGAERRRHRTPQEMISIVQQTFEPGMTVSHVARLHDVNANQLFKWRKQFQEGSLTAITAGEDVVPASELAAAIKQIRDLQRLLGKKTMENEILRD
AVEYGRAKKMDCACALVAGGRRLSAVSQALNVSRAQLSVRVHRKPGWQDGRHHRLYADAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAKR
VYRVMRDHGLLLERKPAAPLTQRAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCSDRETLDWAASTGSYDRATVQDVMLRSVERRFGDALPAS
PVEWLTDNGSAYRAHETRAFARELWLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNFAIAFEYYNEHHPHSALGYHSPREYLRSRVSQ
P
AVEYGRAKKMDCACALVAGGRRLSAVSQALNVSRAQLSVRVHRKPGWQDGRHHRLYADAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAKR
VYRVMRDHGLLLERKPAAPLTQRAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCSDRETLDWAASTGSYDRATVQDVMLRSVERRFGDALPAS
PVEWLTDNGSAYRAHETRAFARELWLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNFAIAFEYYNEHHPHSALGYHSPREYLRSRVSQ
P
Blast result :
Comments
ISAve3 is 95% aa similar to ISAs17.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390
2] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390