ISAri1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Aeromonas rivuli
Aeromonas rivuli
DNA section
IS Length : 1942 bp

Ends


IR Length : 23/28

IRL : TGTCACCGCCGATGTAAAAATGACCCACTAACGCCGGAGCAAAATTGACC
IRR : TGTCAACGCCGACTGAAAATTGACCCACAGGAGGTAAGTTTCGCCGGTTG

Insertion site


Left flankDirect repeatRight flankDR Length
AAGCCAAGGGTGTTCAGTTTGTTTAT6

DNA sequence

TGTCACCGCCGATGTAAAAATGACCCACTAACGCCGGAGCAAAATTGACCCACCTGGACCAAAATCGTGGCTTTTGAGCCGCCTCCATGTTGACCCAGGA
GTTATCAGTGGAAATCAGAGTATTGGCCCGTCAGGGTATGACCATCCGAGCTATCGCCCATCAATTACGGGTGTCCCGTAACACGGTGCGCAAGTATCTG
CGCACCCCGTGCCAGCCAATCTATCCCAGACGAGCTGCCCGCCAGACCAAGCTCGACCCCTTTAAACCCTATTTGCAAGCGCGTATTCAGGCTGCGAAGC
CACACTGGATCCCGGCGACGGTTCTGCTCAGGGAACTGCGAGAGCATGGCTACACTGGCGGGCTCTCCCAGCTCAAGGCCTGGTTATCTCCTCAGCGCCA
TGACTCCCCTGGCACTGTCGTCCGTTTTGAAACGGCACCCGGTAAACAGATGCAGGTCGATTTCACTATCATCCGCCGAGGCAGTCGTCCCCTGAAGGCC
TTTGTGGCGACCCTAGGATTTAGTCGCGCCAGCTATGTTCGCTTCTTCGATCACGAACGTAATGATGCCTGGCTGACCGGGCTGCGTGAGGCTTGTCATT
TCTTCGGTGGGGTTCCGCTGGAGGTGCTATTTGATAATGCCAGCACCATTATTCGCGAGCGGGATGCCTATGGTGAAGGTGATCATCGTTGGCACCCGGC
CTTACTCGCTCTGGCCGAAGAGTTCGGTTTTACTCCCCGCGTCTGCCGTCCCTACCGGGCCCAGACCAAAGGCAAGGTCGAGCGCTTCAACGGCTACCTC
AAGCGCAGTTTTGTCACACCACTGGCAGCCACCCTTAAGCAGGCTGGACTGGCGCTGGATGTGTCAACCGCCAATGCCCACATCGGCCCCTGGCTGCATG
ACATAGCCAACCAGCGTTGCCATGGCACCACCGGCGAGGTCCCGGCTATCCGATTGCAGCAAGAGCGGCCTCATTTTCTACCTCTGCCACAGGCGCTACC
CGTTAGTCCCGGCACCACGGCCACTGCGCGCCCCATGCCGCTGGAGAGCTTGCAGCATCCGCTATCGGTATACCAAGACCTGCTGGAGGTGAACGTATGA
ACTTGCAGCATGAACGCCTGATGGCCCTGAGTCAGGCGCTGCGCTTGGAAGGGATTGCTGCCCAGTGGTCAGCACTAGCTCAGAAGGCGGCCAACGAGGA
GGCGAGCTTCGCCGATTATCTGGAGTGGTTGCTGCACGCAGAACAGACCTTGAGGACCGAGCGCACCCGCCAGACTTTGCTCAAACTGGCCACCTTACCC
GTGGTCAAAACGCTGGAGCAGTACGACTTCGGCTTTGCCAGTGGCGCACCTCGCAGTCAGTTGCAGGAATTGGCCAGCTTGGCCTTTATCGAGCGTAGCG
AAAACATAGTGCTGCTTGGCCCAAGCGGAGTGGGAAAGACACATCTTGCCATCGCGCTGGCCTATAGGGCTCTGATGGCTGGCATCAAGACCAGATTTAT
CAGCGCAGCAGACCTGATGTTGCAGTTGCAAGCAGCTCAGCGACAGGAGCGGCTTAATTCCTACTTCAGCCGTGTCGTGATGGGGCCGCGTTTACTGGTG
ATCGATGAGATAGGCTACTTGCCGTTTGGACGTGAGGAAGCAAACCTGTTTTTCAATGTGATCGCCAAACGCTATGAGAAAGGGAGCCTGATCCTGACAA
GCAATCTGCCATTTAGCCAGTGGGCCAGTGCCTTTGCCGACGATCAGACACTGACGGCAGCCATGCTCGATAGGCTACTACATCATGCGCACATCGTTCA
GATCAGCGGTGAAAGCTATCGTTTGAAAGATAAACGCAAGGCGGGAGTCACCCCCAAAACCAATTAACCCCACGTCTGGGTGGATCAATTTTCAACCGGC
GAAACTTACCTCCTGTGGGTCAATTTTCAGTCGGCGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1014 bp337 aa871100+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTQELSVEIRVLARQGMTIRAIAHQLRVSRNTVRKYLRTPCQPIYPRRAARQTKLDPFKPYLQARIQAAKPHWIPATVLLRELREHGYTGGLSQLKAWL
SPQRHDSPGTVVRFETAPGKQMQVDFTIIRRGSRPLKAFVATLGFSRASYVRFFDHERNDAWLTGLREACHFFGGVPLEVLFDNASTIIRERDAYGEGDH
RWHPALLALAEEFGFTPRVCRPYRAQTKGKVERFNGYLKRSFVTPLAATLKQAGLALDVSTANAHIGPWLHDIANQRCHGTTGEVPAIRLQQERPHFLPL
PQALPVSPGTTATARPMPLESLQHPLSVYQDLLEVNV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
771 bp256 aa10971867+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNLQHERLMALSQALRLEGIAAQWSALAQKAANEEASFADYLEWLLHAEQTLRTERTRQTLLKLATLPVVKTLEQYDFGFASGAPRSQLQELASLAFIER
SENIVLLGPSGVGKTHLAIALAYRALMAGIKTRFISAADLMLQLQAAQRQERLNSYFSRVVMGPRLLVIDEIGYLPFGREEANLFFNVIAKRYEKGSLIL
TSNLPFSQWASAFADDQTLTAAMLDRLLHHAHIVQISGESYRLKDKRKAGVTPKTN

 

Blast result :
Comments
ISAri1 is 95% (istA, the transposase) and 98% (istB, transposition helper) aa similar to ISAeme17.
References
1] Pfeiffer F. (2015) Direct submission