TnAs3
- Family Tn3
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP000645 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 plasmid 4 |
DNA section
IS Length : 18735 bp
Ends
IR Length : 35/38
IRL : GGGGGCACCTCAGAAAACGGAAAATAAAGCACGCTAAGGCATAGCTGACC
IRR : GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGCCGGTTGCAGAG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GTGCGCTGGGC | TACCA | AGCCTGTCAGCA | 5 |
DNA sequence
GGGGGCACCTCAGAAAACGGAAAATAAAGCACGCTAAGGCATAGCTGACCTTGCCAGGCCTGCTTCGCCCTGTAGTGACGCGATCAACGGGCAGGAAACA
TTCCCCTTTCGTGCATGGCAGGCGCACACGAGTTCAGACAGCACGGTTTCCATGCGCGCCAAGTCGGCCATCTTCTCGCGCACGTCCTTGAGCTTGTGTT
CGGCCAGGCTGCTGGCCTCCTCGCAGTGGGTGCCATCGTCGAGCCGCAACAGCTCGGCAATCTCGTCCAGACTGAACCCCAGCCGCTGTGCCGATTTCAC
GAATTTCACCCGAACCACGTCCGCCTCCCCATAGCGGCGGATGCTGCCGTAAGGCTTGTCCGGTTCCCGCAACAGGCCCTTGCGCTGATAGAAGCGGATT
GTCTCCACGTTGACCCCGGCCGCCTTGGCAAAAACGCCAATGGTCAGGTTTTCCAAATTATTTTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTA
AGGTTACGCTATCCAATCCAAATTCAAAAGGGCCAACGTATGTCTGAACCACAAAACGGGCGCGGTGCGCTCTTCGCCGGCGGGCTGGCCGCCATTCTTG
CATCGACCTGCTGCCTGGGGCCGCTAGTACTGGTCGCCCTGGGCTTCTCCGGTGCTTGGATCGGCAACCTGACGGTGCTGGAACCCTATCGACCGTTGTT
CATCGGCGCGGCGCTAGTGGCGCTGTTCTTCGCCTGGAAGCGGATTTACCGGCCCGTGCAGGCATGCAAGCCAGGTGAGGTCTGCGCGATTCCGCAGGTG
CGCGCCACCTACAAGCTGATTTTCTGGATCGTGGCCGTGCTGGTCCTGGTCGCGCTTGGATTTCCCTATGTCGTTCCATTTTTCTATTAACCAGGAGTTC
ATCATGAAGAAACTGTTTGCCTCCCTTGCCCTCGCCGCCGCTGTTGCCCCGGTGTGGGCCGCTACCCAGACCGTCACGCTAGCGGTTCCCGGCATGACTT
GCGCCGCCTGCCCGATCACAGTCAAGAAAGCGCTCTCCAAGGTCGAAGGCGTGAGCAAGGTCGATGTGGGCTTCGAGAAGCGCGAGGCCGTCGTCACTTT
TGACGACACCAAGGCCAGCGTACAGAAGCTGACCAAGGCCACCGCAGACGCCGGCTATCCGTCCAGCGTCAAGCAGTGAGCCAGCAAGCCAACGACAACA
GCGAGAGCCGCTTCATGGGACTGATGACACGCATTGCCGATAAAACCGGCGCGCTCGGCAGCGTCGTTTCCGCGATGGGCTGCGCCGCCTGCTTTCCAGC
CCTCGCCAGCTTCGGCGCGGCCATCGGGCTGGGCTTCTTGAGCCAGTACGAGGGACTGTTCATCAGCCGCCTGCTGCCGCTGTTTGCCGCGCTGGCCTTC
CTGGCGAACGCGCTGGGTTGGTTCAGTCATCGGCAATGGCTGCGCAGTCTGCTCGGCATGATCGGCCCGGCCATCGTGTTTGCGGCCACGGTCTGGCTGC
TCGGCAACTGGTGGACGGCGAACCTGATGTACGTCGGCCTGGCCTTGATGATTGGGGTGTCGATCTGGGACTTCGTGTCGCCGGCGCATCGCCGTTGCGG
ACCGGACGGCTGCGAACTCCCCGCCAAGCGCTTGTGAAAGACGGCTGACCGTGCGACACGGCGGCCCACACGAATAAGGAACGATGGTATGAGCACTCTC
AAAATCACCGGCATGACTTGCGACTCGTGCGCAGTGCATGTCAAGGACGCCCTGGAGAAAGTGCCCGGCGTGCAATCAGCGGATGTCTCCTACGCCAAGG
GCAGCGCCAAGCTCGCCATTGAGGTCGGCACGTCACCCGACGCGCTGACGGCCGCTGTAGCTGGACTCGGTTATCGGGCCACGCTGGCCGATGCCCCCTC
AGTTTCGACGCCGGGCGGATTGCTCGACAAGATGCGCGATCTGCTGGGCAGAAACGACAAGACGGGTAGCAGCGGCGCATTGCATATCGCCGTCATCGGC
AGCGGCGGGGCCGCGATGGCAGCGGCGCTGAAGGCCGTCGAGCAAGGCGCACGTGTCACGCTGATCGAGCGCGGCACCATCGGCGGCACCTGCGTCAATG
TCGGTTGTGTGCCGTCCAAGATCATGATCCGCGCCGCCCATATCGCCCATCTGCGCCGGGAAAGCCCGTTCGATGGCGGCATCGCCGCTACCACGCCGAC
CATCCAGCGCACGGCGCTGCTGGCCCAGCAGCAGGCCCGCGTCGATGAACTGCGCCACGCCAAGTACGAAGGCATCTTGGAGGGCAATCCGGCGATCACT
GTGCTGCACGGCTCCGCCCGCTTTAAGGACAATCGCAACCTGATCGTGCAACTCAACGACGGCGGCGAGCGCGTGGTGGCATTCGACCGCTGCCTGATCG
CCACCGGCGCGAGCCCGGCCGTGCCGCCGATTCCCGGCCTGAAAGACACTCCGTACTGGACTTCCACTGAAGCGCTGGTCAGCGAGACGATTCCTAAGCG
CCTGGCCGTGATTGGCTCATCAGTGGTGGCGCTGGAGCTGGCGCAGGCGTTCGCCCGACTCGGAGCGAAGGTGACGATCCTGGCTCGCAGCACGCTGTTC
TTCCGCGAAGACCCAGCTATAGGCGAAGCCGTCACGGCCGCATTCCGCATGGAGGGCATCGAGGTGAGGGAACACACCCAGGCCAGCCAGGTCGCGTATA
TCAATGGTGAAGGGGACGGCGAATTCGTGCTCACCACGGCGCACGGCGAACTGCGCGCCGACAAGCTGCTGGTCGCCACCGGCCGCGCGCCCAACACACG
CAAGCTGGCACTGGATGCGACGGGCGTCACGCTCACCCCGCAAGGCGCTATCGTCATCGACCCCGGCATGCGTACAAGCGTGGAACACATCTACGCCGCA
GGCGACTGCACCGACCAGCCGCAGTTCGTCTATGTGGCGGCAGCGGCCGGCACTCGCGCCGCGATCAACATGACCGGCGGTGACGCGGCCCTGAACCTGA
CCGCGATGCCGGCCGTGGTGTTCACCGACCCGCAAGTGGCGACCGTAGGCTACAGCGAGGCGGAAGCGCACCATGACGGCATCAAAACTGATAGTCGCAC
GCTAACGCTGGACAACGTGCCGCGCGCGCTCGCCAACTTCGACACGCGCGGCTTCATCAAACTGGTGGTTGAAGAAGGCAGCGGACGACTGATCGGCGTG
CAGGCAGTGGCCCCGGAAGCGGGCGAACTGATCCAGACGGCCGCACTGGCGATTCGCAACCGGATGACGGTGCAGGAACTGGCCGACCAGTTGTTCCCCT
ACCTGACGATGGTCGAAGGGTTGAAGCTCGCGGCGCAGACCTTCAACAAGGATGTGAAGCAGCTTTCCTGCTGCGCCGGGTGAGGACAAGGAGGTGTGCG
ATGAGCGCCTACACGGTATCGCAACTGGCCCATAACGCTGGGGTGAGCGTACATATCGTGCGCGACTACCTGGTGCGCGGCTTGTTACGGCCGGTGGCCT
GCACCACGGGCGGCTACGGCGTGTTCGACGATGCGGCCTTGCAACGGCTGTGCTTCGTGCGCGCGGCCTTCGAGGCGGGTATCGGCCTGGATGCCCTGGC
GCGGCTGTGCCGTGCGCTCGACGCAGCGGACGGCGCACAAGCCGCAGCGCAGCTTGCCGTGCTGCGCCAGTTGGTCGAGCGGCGGCGCGCGGCGTTGGCC
CATCTGGACGCGCAACTGGCCTCCATGCCAGCCGAGCGGGCGCACGAGGAGGCATTGCCGTGAACGCCCCTGACAAACTGCCGCCCGAGACGCGCCAACC
CGTTTCCGGCTACCTGTGGGGTGCGCTGGCCGTGTTGACCTGCCCCTGCCATCTGCCGATTCTCGCCGCCGTGCTGGCCGGGACGACCGCCGGTGCCTTC
CTTGGCGAGCATTGGGGTGTTGCCGCGCTCGCGCTGACCGGCTTGTTCGTTCTGGCCGTAACGCGGCTGCTGCGCGCCTTCCGGGGCGGATCATGACGAG
TTCGCAGCCCGCCGGATGGACGGCGGCCGAGTTGGCGCAGGCGGCGGCGCGCGGACAGCTTGACCTGCATTACCAGCCGCTGGTCGATCTGCGCGATCAC
CGGATCGCTGGCGCGGAAGCGTTGATGCGCTGGCGGCATCCGAGGCTTGGCCTGTTGCCGCCCGGCCAGTTCCTGCCGCTGGCCGAGTCGTTCGGCCTGA
TGCCGGAAATAGGCGCGTGGGTGCTGGGCGAGGCCTGTCGCCAGATGCACAAGTGGCAAGGACCGGCATGGCAACCGTTCCGTCTTGCCATCAATGTGTC
CGCCAGCCAGGTTGGGCCAACGTTCGACGACGAGGTAAAGCGGGTGCTGGCCGATATGGCCCTGCCCGCCGAGCTTCTGGAGATCGAACTGACCGAATCG
GTCGCATTCGGCAATCCAGCCCTGTTCGCCAGTTTCGACGCCTTGCGCGCCATCGGCGTGCGCTTCGCCGCCGACGACTTCGGCACCGGCTATTCCTGCC
TGCAACATCTGAAATGCTGCCCCATCACCACATTGAAAATCGACCAATCCTTTGTCGCCAGGCTCCCGGATGATGCCCGTGACCAAACTATCGTGCGGGC
GGTGATCCAGCTCGCGCACGGGCTGGGCATGGATGTCATTTTCAGAAGACGACTGCACCAGTTGATTGGGCGTAATGGCTGTTGTGCAGCCAGCTCCTGA
CAGTTCAATATCAGAAGTGATCTGCACCAATCTCGACTATGCTCAATACTCGTGTGCACCAAAGCGAGGTGAGCATGGCGACGGACACCCCACGGATTCC
AGAACAAGGCGTGGCCACTCTGCCTGATGAGGCTTGGGAGCGTGCGCGCCGTCGTGCGGAGATCATCAGTCCGTTGGCGCAGTCGGAGACGGTCGGGCAC
GAAGCGGCCGATATGGCGGCTCAGGCGCTGGGCTTGTCTCGGCGCCAGGTATACGTTCTGATCCGGCGTGCCCGGCAAGGCAGCGGCCTCGTGACGGATC
TGGTGCCCGGCCAGTCCGGTGGAGGTAAAGGTAAGGGGCGCTTGCCGGAACCGGTCGAGCGCGTCATCCACGAGCTACTGCAAAAGCGGTTCCTGACCAA
GCAGAAGCGCAGCCTAGCGGCCTTTCACCGCGAAGTCACTCAGGTGTGCAAGGCTCAAAAACTGCGAGTGCCGGCGCGCAATACCGTGGCCTTACGGATC
GCTAGCCTTGACCCGCGCAAGGTCATCCGCCGGCGGGAAGGCCAGGATGCCGCTCGTGACCTACAAGGTGTGGGCGGCGAGCCTCCTGCCGTGACCGCGC
CGCTGGAGCAGGTGCAGATAGACCATACGGTCATCGACCTGATCGTGGTCGATGACCGCGACCGGCAACCTATTGGCCGCCCGTACCTGACCCTCGCCAT
CGACGTGTTCACCCGCTGCGTGCTCGGCATGGTCGTCACGCTGGAAGCGCCGTCTGCCGTTTCGGTTGGCCTGTGCCTCGTGCATGTCGCCTGCGACAAG
CGCCCTTGGCTGGAAGGACTGAACGTGGAAATGGATTGGCAGATGAGCGGCAAGCCCTTGCTGCTCTACCTAGACAACGCGGCCGAGTTCAAGAGCGAGG
CCCTGCGCCGGGGTTGCGAGCAGCATGGCATCCGGCTGGACTATCGCCCGCTGGGACAGCCGCACTATGGCGGCATCGTGGAACGGATCATCGGCACGGC
GATGCAGATGATTCACGACGAACTGCCGGGAACGACCTTCTCCAACCCTGACCAGCGCGGCGACTACGATTCCGAAAACAAGGCCGCCCTGACGCTGCGC
GAGCTAGAGCGCTGGCTCACATTGGCGGTCGGCACCTACCACGGTTCGGTGCACAACGGCCTGCTCCAACCGCCGGCCGCGCGCTGGGCCGAGGCCGTGG
CGCGTGTCGGCGTACCGGCCGTCGTCACACGCGCTACTTCGTTCCTGGTCGATTTTCTGCCGATCCTCCGGCGCACGCTGACCCGCACCGGCTTTGTCAT
CGACCACATCCACTACTACGCCGATGCGCTCAAGCCGTGGATTGCGCGGCGTGAACGCTGGCCGTCCTTTCTGATCCGGCGCGATCCGCGCGACATCAGC
CGTATCTGGGTCCTGGAACCGGAGGGACAGCATTACCTGGAAATTCCCTACCGTACCTTGTCGCATCCGGCTGTCACCCTCTGGGAACAACGGCAGGCGC
TGGCGAAACTGCGGCAGCAAGGGCGCGAACAGGTGGATGAGTCGGCGCTGTTCCGCATGATCGGCCAGATGCGTGAGATTGTGACCAGCGCGCAGAAGGC
CACACGCAAGGCGCGGCGTGACGCGGATCGCCGCCAGCACCTCAAGACATCAGCTCGGCCGGACAAGCCCGTTCCGCCGGATACGGATATTGCCGACCCG
CAGGCAGACAACTTGCCACCCGCCAAACCGTTCGACCAGATTGAGGAGTGGTAGCCGTGGACGAATATCCCATCATCGACCTGTCCCACCTGCTGCCGGC
GGCCCAGGGCTTGGCCCGTCTTCCGGCGGACGAGCGCATCCAGCGCCTTCGCGCCGACCGCTGGATCGGCTATCCGCGCGCAGTCGAGGCGCTGAACCGG
CTGGAAGCCCTTTATGCGTGGCCAAACAAGCAACGCATGCCCAACCTGCTGCTGGTTGGCCCGACCAACAATGGCAAGTCGATGATCGTCGAGAAGTTCC
GCCGCACCCACCCGGCCAGCTCCGACGCCGACCAGGAGCACATCCCGGTGTTGGTCGTGCAGATGCCGTCCGAGCCGTCCGTGATCCGCTTCTACGTCGC
GCTGCTCGCCGCGATGGGCGCGCCGCTGCGCCCACGCCCACGGTTGCCGGAAATGGAGCAACTGGCTCTGGCACTGCTGCGCAAGGTCGGCGTGCGCATG
CTGGTGATCGACGAGCTGCACAACGTGCTGGCCGGCAACAGCGTCAACCGCCGGGAATTCCTCAACCTGCTGCGCTTCCTCGGCAACGAACTGCGCATCC
CGTTGGTTGGGGTAGGCACGCGCGACGCCTACCTAGCCATCCGCTCCGATGACCAGTTGGAAAATCGCTTCGAGCCGATGATGCTGCCGGTATGGGAGGC
CAACGACGATTGCTGCTCACTGCTGGCCAGCTTCGCCGCTTCGCTCCCGCTGCGCCGGCCTTCCCCAATTGCCACGCTGGACATGGCTCGCTACCTGCTC
ACACGCAGCGAGGGCACCATAGGGGAACTGGCGCACTTGCTGATGGCGGCGGCCATCGTCGCCGTGGAGAGCGGCGAGGAAGCGATCAACCATCGCACAC
TCAGCATATCGACAACCTCTCGCGCAACCAAGACATCGCGGTCGGACTGCAAGTGATCTTGAAGCCACGGGCCCGTCCCACCCCGACATGGACCTCGATG
CCCGAACGGACGTTAGATTTCGAGTTCTAGGCGTTCTGCGATGAAGGTTGGATCCCAGCCGGGATTGAAAGTGTCGACGTGGGTGAATCCGAGCCGCTCG
TATAGGCCACGCAGGTTCGGGTGGCAGTCGAGCCGCAGCTTGGCGCACCCCTGCGTTCGCGCGGCATGGCGGCAAGCCTCGATCAGCGCGGAGCTGACAC
CCCGGCCCGCATGTGTCCGTCGCACCGCGAGCTTGTGCAGATATGCGGCCTCCCCCTTGAGGGCGTCGGGCCAGAACTCGGGATCCTCGGCCGACAAGGT
GCAACAGCCGACGATGCCGTCGCTGCAACTCGCGACTAGGAGCTCGGATCTCAGGACGAAGGTCTCCGCGAATGTCCGGTCGATCCGCGCGACGTCCCAG
GCGGGCGTTCCCTTGGCGGACATCCACGCCGCAGCGTCGTGCATCAGCCGCACAACCTCGTCGATATCACCCGAGCAGGCGACCCGAACGTTCGGAGGCT
CCTCGCTGTCCATTCGCTCCCCTGGCGCGGTATGAACCGCCGCCTCATAGTGCAGTTTGATCCTGACGAGCCCAGCATGTCTGCGCCCACCTTCGCGGAA
CCTGACCAGGGTCCGCTAGCGGGCGGCCGGAAGGTGAATGCTAGGCATGATCTAACCCTCGGTCTCTGGCGTCGCGACTGCGAAATTTCGCGAGGGTTTC
CGAGAAGGTGATTGCGCTTCGCAGATCTCCAGGCGCGTGGGTGCGGACGTAGTCAGCGCCATTGCCGATCGCGTGAAGTTCCGCCGCAAGGCTCGCTGGA
CCCAGATCCTTTACAGGAAGGCCAACGGTGGCGCCCAAGAAGGATTTCCGCGACACCGAGACCAATAGCGGAAGCCCCAACGCCGACTTCAGCTTTTGAA
GGTTCGACAGCACGTGCAGCGATGTTTCCGGTGCGGGGCTCAAGAAAAATCCCATCCCCGGATCGAGGATGAGCCGGTCGGCAGCGACCCCGCTCCGTCG
CAAGGCGGAAACCCGCGCCTCGAAGAACCGCACAATCTCGTCGAGCGCGTCTTCGGGTCGAAGGTGACCGGTGCGGGTGGCGATGCCATCCCGCTGCGCT
GAGTGCATAACCACCAGCCTGCAGTCCGCCTCAGCAATATCGGGATAGAGCGCAGGGTCAGGAAATCCTTGGATATCGTTCAGGTAGCCCACGCCGCGCT
TGAGCGCATAGCGCTGGGTTTCCGGTTGGAAGCTGTCGATTGAAACACGGTGCATCTGATCGGACAGGGCGTCTAAGAGCGGCGCAATACGTCTGATCTC
ATCGGCCGGCGATACAGGCCTCGCGTCCGGATGGCTGGCGGCCGGTCCGACATCCACGACGTCTGATCCGACTCGCAGCATTTCGATCGCCGCGGTGACA
GCGCCGGCGGGGTCTAGCCGCCGGCTCTCATCGAAGAAGGAGTCCTCGGTGAGATTCAGAATGCCGAACACCGTCACCATGGCGTCGGCCTCCGCAGCGA
CTTCCACGATGGGGATCGGGCGAGCAAAAAGGCAGCAATTATGAGCCCCATACCTACAAAGCCCCACGCATCAAGCTTTTGCCCATGAAGCAACCAGGCA
ATGGCTGTAATTATGACGACGCCGAGTCCCGACCAGACTGCATAAGCAACACCGACAGGGATGGATTTCAGAACCAGAGAAAGAAAATAAAATGCGATGC
CATAACCGATTATGACAACGGCGGAAGGGGCAAGCTTAGTAAAGCCCTCGCTAGATTTTAATGCGGATGTTGCGATTACTTCGCCAACTATTGCGATAAC
AAGAAAAAGCCAGCCTTTCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCACGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCT
GTGAGCAATTATGTGCTTAGTGCATCTAACGCTTGAGTTAAGCCGCGCCGCGAAGCGGCGTCGGCTTGAACGAATTGTTAGACATTATTTGCCGACTACC
TTGGTGATCTCGCCTTTCACGTAGTGAACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTT
CAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTCTGCCGGTTACTGCGCTGTACCAAAT
GCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGT
TCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCG
TGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTG
CACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCA
TCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCG
GTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCG
ACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACA
AAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACT
TGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGA
AGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTG
CTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCG
GTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTT
CGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCAGGCGCATTGGCCGGACTTTCTCGCGCGCCGTGAGGTAGAAGACCGCCCGCTGTCCGAGTATG
TGCGCGAGGAGTTCGAGACCTACCTGCGCTGCGGCGTGCTCGAGCACGGCTTCCTGCGCGTGGTCTGCGAGCACTGTCGTGCCGAGAGGCTGGTGGCGTA
TTCCTGCAAGAAGCGCGGGCTGTGCCCGAGCTGCGGCGCACGGCGCATGGCCGAGTCGGCGCGGCATCTGGTGGACGAGGTGTTCGGCCCGCGGCCGGTG
CGGCAATGGGTGCTGAGTTTCCCCTACCCGTTGCGCTTCCTGTTCGCCAGCAAGCCGGAGGCGATCGGCCCGGTGCTGGGCATCGTGCATCGTGTGATCG
CCGGTTGGCTTGCCGATCAGGCCGGCGTGCCGCGGGATACGGCGCAATGCGGTGCGGTGACCCTGATCCAGCGCTTCGGCAGCGCGCTGAACCTGAATGT
TCACTTCCACATGCTGTGGCTCGACGGCGTGTACGACGCGAACGTCGAGCCCCCGCGGCGCAAGCCGCGCCTGCGCCGCGCCCGTGCCCCCACCTCTGCG
CAACTGACGCAGCTCGCCAACACCATCGCGCATCGCGTGTGCCGGCACCTGTCGCGCCGCGGCTGGCTCGAAGGCGAAGACGAATCCGTGTTCCTGTCCG
ACAGCGCGGGTAGCGACGACGGCATGGATGGGCTGCGGATGAGTTCGATGACCTACCGCATCGCCACCGGTCGCGACGCTGGCCGCAAGGTCGTCACGCT
GCAAACATTGCCCGGTGACGCAGGCTCGCTGGAGGGCGATGCCGGCAAGGTCGGCGGCTTCTCGCTGCATGCCGGCGTGGCCGCGGAAGCACACGAAAGC
CACAAGCTCGAAAAGCTGTGCCGCTACATCACGCGCCCGGCGATCAGCGAGCAGCGGCTGTCGATCTCACCGCAGGGTAGGGTGCGGTATCAGCTCAAGA
CGCCGTGGCGAAATGGGACCACGCATGTCGAATGGGATGCGGTGGACTTCATCGCCAAGCTGGCGGCACTGGTCCCGCCACCTCGCGCGCATCTCACCCG
CTTCCACGGCGTATTCGCCCCGAATGCAAACCTGCGTGCGCAGCTGACGCCCTCGGGGCGCGGCAAGCGGCCTGCGGGCGATGCGGCGCCAGTGGACGTC
AGCGCCCACGACGAGCCGCGCAGCCCCGAGCAGAAGCGCCGTGCGATGAGCTGGGCGCAACGGCTCAAGCGGGTCTTTTCCATCGACATCACCATCTGCG
CCCACTGCGGCGGCGCGGTACGGATCGTCGCCAGCATCGAGGAACCCACCGCCATCCGCGCCATCCTGGCCCACTTCGAGAAGCACGGTGCGCGGGAAGA
AGCGCACTACCGGCCCGCGGCGCGCGCGCCGCCTGGGGAAGCCGCGTGACCTCCCGCCGGTCACGATGCCGAGGGCGAAAACTGGAAAACGCCACGATGC
GGCCACGACCCCGCAGGGCTGCCCTCGGCCTGCTGCCGGGAATTGGTGGGAAATACCCCCGGACTGCGCCACTGCGCCGCCCCGCGAAGCCGAAACCCCG
CGCGCGGACCACCGACCCGAGCCCCAACCGGCGCTTGCGCGGCCGCAACCTACCCGCCAGACTTCCCGAAAAGGGCGTTTGAAATTCCTATACCCCCTTT
GTGTTGTTCGATAAACTGGGATTTTATTTATTCAGCAAGTCTTGTAATTCATCCAAAAAACGGCAAACATGAAAGCCATCACAAACGGCATGATGCACTT
GAATCGATAAGGGAATATAGTATTTTCCGCCATCCTCATAATACTTCCCAAACGTAAATATCGGCAGTAGATAGTCATATCCTTTTTTTAGATTTAAGTT
AAAGCCTTCAAAGCTTGTCCACGGTATCATAGAAACAGGGAAAGTGTTTTCCGGAGGATTAGGCTTTGCGAACATTCCCTTTCGTTCACCATAAGCGTCT
ATATCCTTTTGATAGTTCTGAAGAAACTCAGTATAGTCTGCTGTAAACTCAGTCCAAATACTCGAAAAGGTTTCAGTTTCCTTATGAAAGATTGTGTAGC
AAGGCAGCATTTCTGAAAAAACACCTACCTGTCCGTTTTTATCTAATGCCGTCCTGAACTCTTCGTGTCGATTGAGGATTGTTGTAACCCCATATAAGAG
AGTCGGATATAATTTTTTTCCATCCTTTTTCAACTTAGAAATATCGAGTTTTACCGTCATACTATATGTGCAGGGCGTATTGTCAAAATAGTGGTCGAAA
TACTCTTTTCGTGTCCAATTATTTATATCAATCTTTGTGAATTGCATCATTTATCCTCCGCAAATTCTAATTTGCAAATTTTATTATACTTGAAAAATAT
GACCAATTCAACCACAGAAAGCGGGGTATCAAAACTATGTATTACACCCAAGAACAGATAGACCGCGCCAACCAAGCCGACCTTGTTTCTTTCCTGCAAT
CACAGGGCGAGCAGCTTACCCGTGCCGGAAATGAATACCGCTGGAAGCGGCACGACAGCCTGACCGTCCGGGGAAACAAATGGTACAGGCACAGCCAGAG
TAAGGGCGGTGCACCCATTGATTTTGTCATGGAGTTTTTCGGCAAGAGCTTCACCGAAGCCGTTGAACTCATAACAAGCCATGAAAACCGCCACTGCGCC
GTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGG
GTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGG
TTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACC
TCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTG
TATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCA
AGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCG
CAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACC
GATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCG
CCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTC
TACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTAGTGAGAGGTAGGGCAGC
GCAAGTCAATCCTGGCGGATTCACTACCCCTGCGCGAAGGCCATCGGTGCCGCATCGAACGGCCGGTTGCGGAAAGTCCTCCCTGCGTCCGCTGATGGCC
GGCAGCAGCCCGTCGTTGCCTGATGGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGCAGCCGTCTTCTGAAAACGACAATGG
AGGTGGTAGCCGAGGGTGTGGAAACACCCGACTGCCTTGCGTGGTTGCGGCAGGCGGGTTGCGACACGGTGCAGGGTTTCCTGTTCGCCAGGCCGATGCC
GGCGGCGGCCTTCGTCGGCTTCGTCAACCAATGGAGGAACACCACCATGAACGCCAATGAACCGAGCACCAGTTGCTGCGTGTGCTGCAAGGAAATCCCG
CTCGATGCCGCCTTCACGCCGGAAGGGGCCGAGTACGTGGAGCATTTCTGCGGGCTGGAGTGCTATCAGCGCTTCCAGGCGCGGGCCAGCACTGCGACCG
AAACCAGCGTCAAACCGGACGCTTGTGATTCGCCGCCGTCAGGTTGAGGCATACCCTAACCTGATGTCAGATGCCATGTGTAAATTGCGTCAGGATAGGA
TTGAATTTTGAATTTATTGACATATCTCGTTGAAGGTCATAGAGTCTTCCCTGACATTTTGCAGGGAATTCCATGACTGGACAGCGCATTGGGTATATCA
GGGTCAGCACCTTCGACCAGAACCCGGAACGGCAACTGGAAGGCGTCAAGGTTGATCGCGCTTTTAGCGACAAGGCATCCGGCAAGGATGTCAAGCGTCC
GCAACTGGAAGCGCTGATAAGCTTCGCCCGCACCGGCGACACCGTGGTGGTGCATAGCATGGATCGCCTGGCGCGCAATCTCGATGATTTGCGCCGGATC
GTGCAAACGCTGACACAACGCGGCGTGCATATCGAATTCGTCAAGGAACACCTCAGTTTTACTGGCGAAGACTCTCCGATGGCGAACCTGATGCTCTCGG
TGATGGGCGCGTTCGCCGAGTTCGAGCGCGCCCTGATCCGCGAGCGTCAGCGCGAGGGTATTGCGCTCGCCAAGCAACGCGGGGCTTACCGTGGCAGGAA
GAAATCCCTGTCGTCTGAGCGTATTGCCGAACTGCGCCAACGTGTCGAGGCTGGCGAGCAAAAGACCAAGCTTGCTCGTGAATTCGGAATCAGTCGCGAA
ACCCTGTATCAATACTTGAGAACGGATCAGTAAATATGCCACGTCGTTCCATCCTGTCCGCCGCCGAGCGGGAAAGCCTGCTGGCGTTGCCGGACTCCAA
GGACGACCTGATCCGACATTACACATTCAACGATACCGACCTCTCGATCATCCGACAGCGGCGCGGGCCAGCCAATCGGCTGGGCTTCGCGGTGCAGCTC
TGTTACCTGCGCTTTCCCGGCGTCATCCTGGGCGTCGATGAACTACCGTTCCCGCCCTTGTTGAAGCTGGTCGCCGACCAGCTCAAGGTCGGCGTCGAAA
GCTGGAACGAGTACGGCCAGCGGGAGCAGACCCGGCGCGAGCACCTGAGCGAGCTGCAAACCGTGTTCGGTTTCCGGCCCTTCACCATGAGCCATTACCG
GCAGGCCGTCCAGATGCTGACCGAGCTGGCGATGCAAACCGACAAAGGCATCGTGCTGGCCAGCGCCTTGATCGGGCACCTGCGGCGGCAGTCGGTCATT
CTGCCCGCCCTCAACGCCGTCGAGCGGGCGAGTGCCGAGGCGATCACCCGTGCTAACCGGCGCATCTACGACGCCTTGGCCGAACCACTGGCGGACGCGC
ATCGCCGCCGCCTCGACGATCTGCTCAAGCGCCGGGACAACGGCAAGACGACCTGGTTGGCTTGGTTGCGCCAGTCTCCGGCCAAGCCAAATTCGCGGCA
TATGCTGGAACACATCGAACGCCTCAAGGCATGGCAGGCACTCGATCTGCCTACCGGCATCGAGCGGCTGGTTCACCAGAACCGCCTGCTCAAGATTGCC
CGCGAGGGCGGCCAGATGACACCCGCCGACCTGGCCAAATTCGAGCCGCAACGGCGCTACGCCACTCTCGTGGCGCTGGCCACCGAGGGCATGGCCACCG
TCACCGACGAAATCATCGACCTGCACGACCGCATCCTGGGTAAGCTGTTTAACGCTGCCAAGAATAAGCATCAGCAGCAGTTCCAGGCGTCAGGCAAGGC
CATCAACGCCAAGGTACGTCTGTACGGGCGCATCGGTCAGGCGCTGATCGACGCCAAGCAATCAGGCCGCGATGCGTTTGCCGCCATCGAGGCCGTCATG
TCCTGGGATTCCTTTGCCGAGAGCGTCACCGAGGCGCAGAAGCTCGCGCAACCCGATGACTTCGATTTCCTGCATCGCATCGGCGAGAGCTACGCCACCC
TGCGCCGCTATGCACCGGAATTCCTTGCCGTGCTCAAGCTGCGGGCCGCGCCCGCCGCCAAAAACGTGCTTGATGCCATTGAGGTGCTGCGCGGCATGAA
CACCGACAACGCCCGCAAGCTGCCAGCCGATGCACCGACCGGCTTCATCAAGCCGCGCTGGCAGAAACTGGTGATGACCGACGCCGGCATCGACCGGCGC
TACTACGAACTGTGCGCGCTGTCCGAGTTGAAGAACTCCCTGCGCTCGGGCGACATCTGGGTGCAGGGTTCACGCCAGTTCAAGGACTTCGAGGACTACC
TGGTACCGCCCGAGAAGTTCACCAGCCTCAAGCAGTCCAGCGAATTGCCGCTGGCCGTGGCCACCGACTGCGAACAATATCTGCATGAGCGGCTGACGCT
GCTGGAAGCACAACTTGCCACCGTCAACCGCATGGCGGCAGCCAACGACCTGCCGGATGCCATCATCACCGAGTCGGGCTTGAAGATCACGCCGCTGGAT
GCGGCGGTGCCCGACACCGCGCAGGCGCTGATAGACCAGACAGCCATGGTCCTGCCGCACGTCAAGATCACCGAACTGCTGCTCGAAGTCGATGAGTGGA
CGGGCTTCACCCGGCACTTCACGCACTTGAAATCGGGCGATCTGGCCAAGGACAAGAACCTGTTGTTGACCACGATCCTGGCCGACGCGATCAACCTGGG
CCTGACCAAGATGGCCGAGTCCTGCCCCGGCACGACCTACGCGAAGCTCGCTTGGCTGCAAGCCTGGCATACCCGCGACGAAACGTACTCGACAGCGTTG
GCTGAACTGGTCAACGCTCAGTTTCGGCATCCCTTTGCCGGGCACTGGGGCGATGGCACCACATCATCATCGGACGGACAGAATTTCCGAACCGCTAGCA
AGGCAAAGAGCACGGGGCACATCAACCCAAAATATGGCAGCAGCCCAGGACGGACTTTCTACACCCACATCTCCGACCAATACGCGCCATTCCACACCAA
GGTGGTCAATGTCGGCCTGCGCGACTCAACCTACGTGCTCGACGGCCTGCTGTACCACGAATCCGACCTGCGGATCGAGGAGCACTACACCGACACGGCG
GGCTTCACCGATCACGTCTTCGCCCTGATGCACCTCTTGGGCTTCCGCTTCGCGCCGCGCATCCGCGACCTGGGCGACACCAAGCTCTACATCCCGAAGG
GCGATGCCGCCTATGACGCGCTCAAGCCGATGATCGGCGGCACGCTCAACATCAAGCACGTCCGCGCCCATTGGGACGAAATCCTGCGGCTGGCCACCTC
GATCAAGCAGGGCACGGTGACGGCCTCGCTGATGCTCAGGAAACTCGGCAGCTACCCGCGCCAGAACGGCTTGGCCGTCGCGCTGCGCGAGTTGGGCCGC
ATCGAGCGCACGCTGTTCATCCTCGACTGGCTGCAAAGCGTCGAGCTACGCCGCCGCGTGCATGCCGGGCTGAACAAGGGCGAGGCGCGCAATGCGCTGG
CCCGTGCCGTGTTCTTCAACCGCCTTGGTGAAATCCGTGACCGCAGTTTCGAGCAGCAGCGCTACCGGGCCAGCGGCCTCAACCTGGTGACGGCGGCCAT
CGTGCTGTGGAACACGGTCTACCTGGAGCGTGCGGCGCATGCGTTGCGCGGCAATGGTCATGCCGTCGATGACTCGCTATTGCAGTACCTGTCGCCACTC
GGCTGGGAGCACATCAACCTGACCGGTGATTACCTATGGCGCAGCAGCGCCAAGATCGGCGCGGGGAAGTTCAGGCCGCTACGGCCTCTGCAACCGGCTT
AGCGTGCTTTATTTTCCGTTTTCTGAGACGACCCC
TTCCCCTTTCGTGCATGGCAGGCGCACACGAGTTCAGACAGCACGGTTTCCATGCGCGCCAAGTCGGCCATCTTCTCGCGCACGTCCTTGAGCTTGTGTT
CGGCCAGGCTGCTGGCCTCCTCGCAGTGGGTGCCATCGTCGAGCCGCAACAGCTCGGCAATCTCGTCCAGACTGAACCCCAGCCGCTGTGCCGATTTCAC
GAATTTCACCCGAACCACGTCCGCCTCCCCATAGCGGCGGATGCTGCCGTAAGGCTTGTCCGGTTCCCGCAACAGGCCCTTGCGCTGATAGAAGCGGATT
GTCTCCACGTTGACCCCGGCCGCCTTGGCAAAAACGCCAATGGTCAGGTTTTCCAAATTATTTTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTA
AGGTTACGCTATCCAATCCAAATTCAAAAGGGCCAACGTATGTCTGAACCACAAAACGGGCGCGGTGCGCTCTTCGCCGGCGGGCTGGCCGCCATTCTTG
CATCGACCTGCTGCCTGGGGCCGCTAGTACTGGTCGCCCTGGGCTTCTCCGGTGCTTGGATCGGCAACCTGACGGTGCTGGAACCCTATCGACCGTTGTT
CATCGGCGCGGCGCTAGTGGCGCTGTTCTTCGCCTGGAAGCGGATTTACCGGCCCGTGCAGGCATGCAAGCCAGGTGAGGTCTGCGCGATTCCGCAGGTG
CGCGCCACCTACAAGCTGATTTTCTGGATCGTGGCCGTGCTGGTCCTGGTCGCGCTTGGATTTCCCTATGTCGTTCCATTTTTCTATTAACCAGGAGTTC
ATCATGAAGAAACTGTTTGCCTCCCTTGCCCTCGCCGCCGCTGTTGCCCCGGTGTGGGCCGCTACCCAGACCGTCACGCTAGCGGTTCCCGGCATGACTT
GCGCCGCCTGCCCGATCACAGTCAAGAAAGCGCTCTCCAAGGTCGAAGGCGTGAGCAAGGTCGATGTGGGCTTCGAGAAGCGCGAGGCCGTCGTCACTTT
TGACGACACCAAGGCCAGCGTACAGAAGCTGACCAAGGCCACCGCAGACGCCGGCTATCCGTCCAGCGTCAAGCAGTGAGCCAGCAAGCCAACGACAACA
GCGAGAGCCGCTTCATGGGACTGATGACACGCATTGCCGATAAAACCGGCGCGCTCGGCAGCGTCGTTTCCGCGATGGGCTGCGCCGCCTGCTTTCCAGC
CCTCGCCAGCTTCGGCGCGGCCATCGGGCTGGGCTTCTTGAGCCAGTACGAGGGACTGTTCATCAGCCGCCTGCTGCCGCTGTTTGCCGCGCTGGCCTTC
CTGGCGAACGCGCTGGGTTGGTTCAGTCATCGGCAATGGCTGCGCAGTCTGCTCGGCATGATCGGCCCGGCCATCGTGTTTGCGGCCACGGTCTGGCTGC
TCGGCAACTGGTGGACGGCGAACCTGATGTACGTCGGCCTGGCCTTGATGATTGGGGTGTCGATCTGGGACTTCGTGTCGCCGGCGCATCGCCGTTGCGG
ACCGGACGGCTGCGAACTCCCCGCCAAGCGCTTGTGAAAGACGGCTGACCGTGCGACACGGCGGCCCACACGAATAAGGAACGATGGTATGAGCACTCTC
AAAATCACCGGCATGACTTGCGACTCGTGCGCAGTGCATGTCAAGGACGCCCTGGAGAAAGTGCCCGGCGTGCAATCAGCGGATGTCTCCTACGCCAAGG
GCAGCGCCAAGCTCGCCATTGAGGTCGGCACGTCACCCGACGCGCTGACGGCCGCTGTAGCTGGACTCGGTTATCGGGCCACGCTGGCCGATGCCCCCTC
AGTTTCGACGCCGGGCGGATTGCTCGACAAGATGCGCGATCTGCTGGGCAGAAACGACAAGACGGGTAGCAGCGGCGCATTGCATATCGCCGTCATCGGC
AGCGGCGGGGCCGCGATGGCAGCGGCGCTGAAGGCCGTCGAGCAAGGCGCACGTGTCACGCTGATCGAGCGCGGCACCATCGGCGGCACCTGCGTCAATG
TCGGTTGTGTGCCGTCCAAGATCATGATCCGCGCCGCCCATATCGCCCATCTGCGCCGGGAAAGCCCGTTCGATGGCGGCATCGCCGCTACCACGCCGAC
CATCCAGCGCACGGCGCTGCTGGCCCAGCAGCAGGCCCGCGTCGATGAACTGCGCCACGCCAAGTACGAAGGCATCTTGGAGGGCAATCCGGCGATCACT
GTGCTGCACGGCTCCGCCCGCTTTAAGGACAATCGCAACCTGATCGTGCAACTCAACGACGGCGGCGAGCGCGTGGTGGCATTCGACCGCTGCCTGATCG
CCACCGGCGCGAGCCCGGCCGTGCCGCCGATTCCCGGCCTGAAAGACACTCCGTACTGGACTTCCACTGAAGCGCTGGTCAGCGAGACGATTCCTAAGCG
CCTGGCCGTGATTGGCTCATCAGTGGTGGCGCTGGAGCTGGCGCAGGCGTTCGCCCGACTCGGAGCGAAGGTGACGATCCTGGCTCGCAGCACGCTGTTC
TTCCGCGAAGACCCAGCTATAGGCGAAGCCGTCACGGCCGCATTCCGCATGGAGGGCATCGAGGTGAGGGAACACACCCAGGCCAGCCAGGTCGCGTATA
TCAATGGTGAAGGGGACGGCGAATTCGTGCTCACCACGGCGCACGGCGAACTGCGCGCCGACAAGCTGCTGGTCGCCACCGGCCGCGCGCCCAACACACG
CAAGCTGGCACTGGATGCGACGGGCGTCACGCTCACCCCGCAAGGCGCTATCGTCATCGACCCCGGCATGCGTACAAGCGTGGAACACATCTACGCCGCA
GGCGACTGCACCGACCAGCCGCAGTTCGTCTATGTGGCGGCAGCGGCCGGCACTCGCGCCGCGATCAACATGACCGGCGGTGACGCGGCCCTGAACCTGA
CCGCGATGCCGGCCGTGGTGTTCACCGACCCGCAAGTGGCGACCGTAGGCTACAGCGAGGCGGAAGCGCACCATGACGGCATCAAAACTGATAGTCGCAC
GCTAACGCTGGACAACGTGCCGCGCGCGCTCGCCAACTTCGACACGCGCGGCTTCATCAAACTGGTGGTTGAAGAAGGCAGCGGACGACTGATCGGCGTG
CAGGCAGTGGCCCCGGAAGCGGGCGAACTGATCCAGACGGCCGCACTGGCGATTCGCAACCGGATGACGGTGCAGGAACTGGCCGACCAGTTGTTCCCCT
ACCTGACGATGGTCGAAGGGTTGAAGCTCGCGGCGCAGACCTTCAACAAGGATGTGAAGCAGCTTTCCTGCTGCGCCGGGTGAGGACAAGGAGGTGTGCG
ATGAGCGCCTACACGGTATCGCAACTGGCCCATAACGCTGGGGTGAGCGTACATATCGTGCGCGACTACCTGGTGCGCGGCTTGTTACGGCCGGTGGCCT
GCACCACGGGCGGCTACGGCGTGTTCGACGATGCGGCCTTGCAACGGCTGTGCTTCGTGCGCGCGGCCTTCGAGGCGGGTATCGGCCTGGATGCCCTGGC
GCGGCTGTGCCGTGCGCTCGACGCAGCGGACGGCGCACAAGCCGCAGCGCAGCTTGCCGTGCTGCGCCAGTTGGTCGAGCGGCGGCGCGCGGCGTTGGCC
CATCTGGACGCGCAACTGGCCTCCATGCCAGCCGAGCGGGCGCACGAGGAGGCATTGCCGTGAACGCCCCTGACAAACTGCCGCCCGAGACGCGCCAACC
CGTTTCCGGCTACCTGTGGGGTGCGCTGGCCGTGTTGACCTGCCCCTGCCATCTGCCGATTCTCGCCGCCGTGCTGGCCGGGACGACCGCCGGTGCCTTC
CTTGGCGAGCATTGGGGTGTTGCCGCGCTCGCGCTGACCGGCTTGTTCGTTCTGGCCGTAACGCGGCTGCTGCGCGCCTTCCGGGGCGGATCATGACGAG
TTCGCAGCCCGCCGGATGGACGGCGGCCGAGTTGGCGCAGGCGGCGGCGCGCGGACAGCTTGACCTGCATTACCAGCCGCTGGTCGATCTGCGCGATCAC
CGGATCGCTGGCGCGGAAGCGTTGATGCGCTGGCGGCATCCGAGGCTTGGCCTGTTGCCGCCCGGCCAGTTCCTGCCGCTGGCCGAGTCGTTCGGCCTGA
TGCCGGAAATAGGCGCGTGGGTGCTGGGCGAGGCCTGTCGCCAGATGCACAAGTGGCAAGGACCGGCATGGCAACCGTTCCGTCTTGCCATCAATGTGTC
CGCCAGCCAGGTTGGGCCAACGTTCGACGACGAGGTAAAGCGGGTGCTGGCCGATATGGCCCTGCCCGCCGAGCTTCTGGAGATCGAACTGACCGAATCG
GTCGCATTCGGCAATCCAGCCCTGTTCGCCAGTTTCGACGCCTTGCGCGCCATCGGCGTGCGCTTCGCCGCCGACGACTTCGGCACCGGCTATTCCTGCC
TGCAACATCTGAAATGCTGCCCCATCACCACATTGAAAATCGACCAATCCTTTGTCGCCAGGCTCCCGGATGATGCCCGTGACCAAACTATCGTGCGGGC
GGTGATCCAGCTCGCGCACGGGCTGGGCATGGATGTCATTTTCAGAAGACGACTGCACCAGTTGATTGGGCGTAATGGCTGTTGTGCAGCCAGCTCCTGA
CAGTTCAATATCAGAAGTGATCTGCACCAATCTCGACTATGCTCAATACTCGTGTGCACCAAAGCGAGGTGAGCATGGCGACGGACACCCCACGGATTCC
AGAACAAGGCGTGGCCACTCTGCCTGATGAGGCTTGGGAGCGTGCGCGCCGTCGTGCGGAGATCATCAGTCCGTTGGCGCAGTCGGAGACGGTCGGGCAC
GAAGCGGCCGATATGGCGGCTCAGGCGCTGGGCTTGTCTCGGCGCCAGGTATACGTTCTGATCCGGCGTGCCCGGCAAGGCAGCGGCCTCGTGACGGATC
TGGTGCCCGGCCAGTCCGGTGGAGGTAAAGGTAAGGGGCGCTTGCCGGAACCGGTCGAGCGCGTCATCCACGAGCTACTGCAAAAGCGGTTCCTGACCAA
GCAGAAGCGCAGCCTAGCGGCCTTTCACCGCGAAGTCACTCAGGTGTGCAAGGCTCAAAAACTGCGAGTGCCGGCGCGCAATACCGTGGCCTTACGGATC
GCTAGCCTTGACCCGCGCAAGGTCATCCGCCGGCGGGAAGGCCAGGATGCCGCTCGTGACCTACAAGGTGTGGGCGGCGAGCCTCCTGCCGTGACCGCGC
CGCTGGAGCAGGTGCAGATAGACCATACGGTCATCGACCTGATCGTGGTCGATGACCGCGACCGGCAACCTATTGGCCGCCCGTACCTGACCCTCGCCAT
CGACGTGTTCACCCGCTGCGTGCTCGGCATGGTCGTCACGCTGGAAGCGCCGTCTGCCGTTTCGGTTGGCCTGTGCCTCGTGCATGTCGCCTGCGACAAG
CGCCCTTGGCTGGAAGGACTGAACGTGGAAATGGATTGGCAGATGAGCGGCAAGCCCTTGCTGCTCTACCTAGACAACGCGGCCGAGTTCAAGAGCGAGG
CCCTGCGCCGGGGTTGCGAGCAGCATGGCATCCGGCTGGACTATCGCCCGCTGGGACAGCCGCACTATGGCGGCATCGTGGAACGGATCATCGGCACGGC
GATGCAGATGATTCACGACGAACTGCCGGGAACGACCTTCTCCAACCCTGACCAGCGCGGCGACTACGATTCCGAAAACAAGGCCGCCCTGACGCTGCGC
GAGCTAGAGCGCTGGCTCACATTGGCGGTCGGCACCTACCACGGTTCGGTGCACAACGGCCTGCTCCAACCGCCGGCCGCGCGCTGGGCCGAGGCCGTGG
CGCGTGTCGGCGTACCGGCCGTCGTCACACGCGCTACTTCGTTCCTGGTCGATTTTCTGCCGATCCTCCGGCGCACGCTGACCCGCACCGGCTTTGTCAT
CGACCACATCCACTACTACGCCGATGCGCTCAAGCCGTGGATTGCGCGGCGTGAACGCTGGCCGTCCTTTCTGATCCGGCGCGATCCGCGCGACATCAGC
CGTATCTGGGTCCTGGAACCGGAGGGACAGCATTACCTGGAAATTCCCTACCGTACCTTGTCGCATCCGGCTGTCACCCTCTGGGAACAACGGCAGGCGC
TGGCGAAACTGCGGCAGCAAGGGCGCGAACAGGTGGATGAGTCGGCGCTGTTCCGCATGATCGGCCAGATGCGTGAGATTGTGACCAGCGCGCAGAAGGC
CACACGCAAGGCGCGGCGTGACGCGGATCGCCGCCAGCACCTCAAGACATCAGCTCGGCCGGACAAGCCCGTTCCGCCGGATACGGATATTGCCGACCCG
CAGGCAGACAACTTGCCACCCGCCAAACCGTTCGACCAGATTGAGGAGTGGTAGCCGTGGACGAATATCCCATCATCGACCTGTCCCACCTGCTGCCGGC
GGCCCAGGGCTTGGCCCGTCTTCCGGCGGACGAGCGCATCCAGCGCCTTCGCGCCGACCGCTGGATCGGCTATCCGCGCGCAGTCGAGGCGCTGAACCGG
CTGGAAGCCCTTTATGCGTGGCCAAACAAGCAACGCATGCCCAACCTGCTGCTGGTTGGCCCGACCAACAATGGCAAGTCGATGATCGTCGAGAAGTTCC
GCCGCACCCACCCGGCCAGCTCCGACGCCGACCAGGAGCACATCCCGGTGTTGGTCGTGCAGATGCCGTCCGAGCCGTCCGTGATCCGCTTCTACGTCGC
GCTGCTCGCCGCGATGGGCGCGCCGCTGCGCCCACGCCCACGGTTGCCGGAAATGGAGCAACTGGCTCTGGCACTGCTGCGCAAGGTCGGCGTGCGCATG
CTGGTGATCGACGAGCTGCACAACGTGCTGGCCGGCAACAGCGTCAACCGCCGGGAATTCCTCAACCTGCTGCGCTTCCTCGGCAACGAACTGCGCATCC
CGTTGGTTGGGGTAGGCACGCGCGACGCCTACCTAGCCATCCGCTCCGATGACCAGTTGGAAAATCGCTTCGAGCCGATGATGCTGCCGGTATGGGAGGC
CAACGACGATTGCTGCTCACTGCTGGCCAGCTTCGCCGCTTCGCTCCCGCTGCGCCGGCCTTCCCCAATTGCCACGCTGGACATGGCTCGCTACCTGCTC
ACACGCAGCGAGGGCACCATAGGGGAACTGGCGCACTTGCTGATGGCGGCGGCCATCGTCGCCGTGGAGAGCGGCGAGGAAGCGATCAACCATCGCACAC
TCAGCATATCGACAACCTCTCGCGCAACCAAGACATCGCGGTCGGACTGCAAGTGATCTTGAAGCCACGGGCCCGTCCCACCCCGACATGGACCTCGATG
CCCGAACGGACGTTAGATTTCGAGTTCTAGGCGTTCTGCGATGAAGGTTGGATCCCAGCCGGGATTGAAAGTGTCGACGTGGGTGAATCCGAGCCGCTCG
TATAGGCCACGCAGGTTCGGGTGGCAGTCGAGCCGCAGCTTGGCGCACCCCTGCGTTCGCGCGGCATGGCGGCAAGCCTCGATCAGCGCGGAGCTGACAC
CCCGGCCCGCATGTGTCCGTCGCACCGCGAGCTTGTGCAGATATGCGGCCTCCCCCTTGAGGGCGTCGGGCCAGAACTCGGGATCCTCGGCCGACAAGGT
GCAACAGCCGACGATGCCGTCGCTGCAACTCGCGACTAGGAGCTCGGATCTCAGGACGAAGGTCTCCGCGAATGTCCGGTCGATCCGCGCGACGTCCCAG
GCGGGCGTTCCCTTGGCGGACATCCACGCCGCAGCGTCGTGCATCAGCCGCACAACCTCGTCGATATCACCCGAGCAGGCGACCCGAACGTTCGGAGGCT
CCTCGCTGTCCATTCGCTCCCCTGGCGCGGTATGAACCGCCGCCTCATAGTGCAGTTTGATCCTGACGAGCCCAGCATGTCTGCGCCCACCTTCGCGGAA
CCTGACCAGGGTCCGCTAGCGGGCGGCCGGAAGGTGAATGCTAGGCATGATCTAACCCTCGGTCTCTGGCGTCGCGACTGCGAAATTTCGCGAGGGTTTC
CGAGAAGGTGATTGCGCTTCGCAGATCTCCAGGCGCGTGGGTGCGGACGTAGTCAGCGCCATTGCCGATCGCGTGAAGTTCCGCCGCAAGGCTCGCTGGA
CCCAGATCCTTTACAGGAAGGCCAACGGTGGCGCCCAAGAAGGATTTCCGCGACACCGAGACCAATAGCGGAAGCCCCAACGCCGACTTCAGCTTTTGAA
GGTTCGACAGCACGTGCAGCGATGTTTCCGGTGCGGGGCTCAAGAAAAATCCCATCCCCGGATCGAGGATGAGCCGGTCGGCAGCGACCCCGCTCCGTCG
CAAGGCGGAAACCCGCGCCTCGAAGAACCGCACAATCTCGTCGAGCGCGTCTTCGGGTCGAAGGTGACCGGTGCGGGTGGCGATGCCATCCCGCTGCGCT
GAGTGCATAACCACCAGCCTGCAGTCCGCCTCAGCAATATCGGGATAGAGCGCAGGGTCAGGAAATCCTTGGATATCGTTCAGGTAGCCCACGCCGCGCT
TGAGCGCATAGCGCTGGGTTTCCGGTTGGAAGCTGTCGATTGAAACACGGTGCATCTGATCGGACAGGGCGTCTAAGAGCGGCGCAATACGTCTGATCTC
ATCGGCCGGCGATACAGGCCTCGCGTCCGGATGGCTGGCGGCCGGTCCGACATCCACGACGTCTGATCCGACTCGCAGCATTTCGATCGCCGCGGTGACA
GCGCCGGCGGGGTCTAGCCGCCGGCTCTCATCGAAGAAGGAGTCCTCGGTGAGATTCAGAATGCCGAACACCGTCACCATGGCGTCGGCCTCCGCAGCGA
CTTCCACGATGGGGATCGGGCGAGCAAAAAGGCAGCAATTATGAGCCCCATACCTACAAAGCCCCACGCATCAAGCTTTTGCCCATGAAGCAACCAGGCA
ATGGCTGTAATTATGACGACGCCGAGTCCCGACCAGACTGCATAAGCAACACCGACAGGGATGGATTTCAGAACCAGAGAAAGAAAATAAAATGCGATGC
CATAACCGATTATGACAACGGCGGAAGGGGCAAGCTTAGTAAAGCCCTCGCTAGATTTTAATGCGGATGTTGCGATTACTTCGCCAACTATTGCGATAAC
AAGAAAAAGCCAGCCTTTCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCACGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCT
GTGAGCAATTATGTGCTTAGTGCATCTAACGCTTGAGTTAAGCCGCGCCGCGAAGCGGCGTCGGCTTGAACGAATTGTTAGACATTATTTGCCGACTACC
TTGGTGATCTCGCCTTTCACGTAGTGAACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTT
CAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTCTGCCGGTTACTGCGCTGTACCAAAT
GCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGT
TCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCG
TGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTG
CACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCA
TCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCG
GTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCG
ACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACA
AAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACT
TGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGA
AGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTG
CTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCG
GTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTT
CGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCAGGCGCATTGGCCGGACTTTCTCGCGCGCCGTGAGGTAGAAGACCGCCCGCTGTCCGAGTATG
TGCGCGAGGAGTTCGAGACCTACCTGCGCTGCGGCGTGCTCGAGCACGGCTTCCTGCGCGTGGTCTGCGAGCACTGTCGTGCCGAGAGGCTGGTGGCGTA
TTCCTGCAAGAAGCGCGGGCTGTGCCCGAGCTGCGGCGCACGGCGCATGGCCGAGTCGGCGCGGCATCTGGTGGACGAGGTGTTCGGCCCGCGGCCGGTG
CGGCAATGGGTGCTGAGTTTCCCCTACCCGTTGCGCTTCCTGTTCGCCAGCAAGCCGGAGGCGATCGGCCCGGTGCTGGGCATCGTGCATCGTGTGATCG
CCGGTTGGCTTGCCGATCAGGCCGGCGTGCCGCGGGATACGGCGCAATGCGGTGCGGTGACCCTGATCCAGCGCTTCGGCAGCGCGCTGAACCTGAATGT
TCACTTCCACATGCTGTGGCTCGACGGCGTGTACGACGCGAACGTCGAGCCCCCGCGGCGCAAGCCGCGCCTGCGCCGCGCCCGTGCCCCCACCTCTGCG
CAACTGACGCAGCTCGCCAACACCATCGCGCATCGCGTGTGCCGGCACCTGTCGCGCCGCGGCTGGCTCGAAGGCGAAGACGAATCCGTGTTCCTGTCCG
ACAGCGCGGGTAGCGACGACGGCATGGATGGGCTGCGGATGAGTTCGATGACCTACCGCATCGCCACCGGTCGCGACGCTGGCCGCAAGGTCGTCACGCT
GCAAACATTGCCCGGTGACGCAGGCTCGCTGGAGGGCGATGCCGGCAAGGTCGGCGGCTTCTCGCTGCATGCCGGCGTGGCCGCGGAAGCACACGAAAGC
CACAAGCTCGAAAAGCTGTGCCGCTACATCACGCGCCCGGCGATCAGCGAGCAGCGGCTGTCGATCTCACCGCAGGGTAGGGTGCGGTATCAGCTCAAGA
CGCCGTGGCGAAATGGGACCACGCATGTCGAATGGGATGCGGTGGACTTCATCGCCAAGCTGGCGGCACTGGTCCCGCCACCTCGCGCGCATCTCACCCG
CTTCCACGGCGTATTCGCCCCGAATGCAAACCTGCGTGCGCAGCTGACGCCCTCGGGGCGCGGCAAGCGGCCTGCGGGCGATGCGGCGCCAGTGGACGTC
AGCGCCCACGACGAGCCGCGCAGCCCCGAGCAGAAGCGCCGTGCGATGAGCTGGGCGCAACGGCTCAAGCGGGTCTTTTCCATCGACATCACCATCTGCG
CCCACTGCGGCGGCGCGGTACGGATCGTCGCCAGCATCGAGGAACCCACCGCCATCCGCGCCATCCTGGCCCACTTCGAGAAGCACGGTGCGCGGGAAGA
AGCGCACTACCGGCCCGCGGCGCGCGCGCCGCCTGGGGAAGCCGCGTGACCTCCCGCCGGTCACGATGCCGAGGGCGAAAACTGGAAAACGCCACGATGC
GGCCACGACCCCGCAGGGCTGCCCTCGGCCTGCTGCCGGGAATTGGTGGGAAATACCCCCGGACTGCGCCACTGCGCCGCCCCGCGAAGCCGAAACCCCG
CGCGCGGACCACCGACCCGAGCCCCAACCGGCGCTTGCGCGGCCGCAACCTACCCGCCAGACTTCCCGAAAAGGGCGTTTGAAATTCCTATACCCCCTTT
GTGTTGTTCGATAAACTGGGATTTTATTTATTCAGCAAGTCTTGTAATTCATCCAAAAAACGGCAAACATGAAAGCCATCACAAACGGCATGATGCACTT
GAATCGATAAGGGAATATAGTATTTTCCGCCATCCTCATAATACTTCCCAAACGTAAATATCGGCAGTAGATAGTCATATCCTTTTTTTAGATTTAAGTT
AAAGCCTTCAAAGCTTGTCCACGGTATCATAGAAACAGGGAAAGTGTTTTCCGGAGGATTAGGCTTTGCGAACATTCCCTTTCGTTCACCATAAGCGTCT
ATATCCTTTTGATAGTTCTGAAGAAACTCAGTATAGTCTGCTGTAAACTCAGTCCAAATACTCGAAAAGGTTTCAGTTTCCTTATGAAAGATTGTGTAGC
AAGGCAGCATTTCTGAAAAAACACCTACCTGTCCGTTTTTATCTAATGCCGTCCTGAACTCTTCGTGTCGATTGAGGATTGTTGTAACCCCATATAAGAG
AGTCGGATATAATTTTTTTCCATCCTTTTTCAACTTAGAAATATCGAGTTTTACCGTCATACTATATGTGCAGGGCGTATTGTCAAAATAGTGGTCGAAA
TACTCTTTTCGTGTCCAATTATTTATATCAATCTTTGTGAATTGCATCATTTATCCTCCGCAAATTCTAATTTGCAAATTTTATTATACTTGAAAAATAT
GACCAATTCAACCACAGAAAGCGGGGTATCAAAACTATGTATTACACCCAAGAACAGATAGACCGCGCCAACCAAGCCGACCTTGTTTCTTTCCTGCAAT
CACAGGGCGAGCAGCTTACCCGTGCCGGAAATGAATACCGCTGGAAGCGGCACGACAGCCTGACCGTCCGGGGAAACAAATGGTACAGGCACAGCCAGAG
TAAGGGCGGTGCACCCATTGATTTTGTCATGGAGTTTTTCGGCAAGAGCTTCACCGAAGCCGTTGAACTCATAACAAGCCATGAAAACCGCCACTGCGCC
GTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGG
GTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGG
TTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACC
TCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTG
TATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCA
AGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGCTGTCGCGTGCACGGGCATGGTGGCTGAAGGACCAGGCCGAGGGCCG
CAGCGGCGTTGCGCTTCCCGACGCCCTTGAGCGGAAGTATCCGCGCGCCGGGCATTCCTGGCCGTGGTTCTGGGTTTTTGCGCAGCACACGCATTCGACC
GATCCACGGAGCGGTGTCGTGCGTCGCCATCACATGTATGACCAGACCTTTCAGCGCGCCTTCAAACGTGCCGTAGAACAAGCAGGCATCACGAAGCCCG
CCACACCGCACACCCTCCGCCACTCGTTCGCGACGGCCTTGCTCCGCAGCGGTTACGACATTCGAACCGTGCAGGATCTGCTCGGCCATTCCGACGTCTC
TACGACGATGATTTACACGCATGTGCTGAAAGTTGGCGGTGCCGGAGTGCGCTCACCGCTTGATGCGCTGCCGCCCCTCACTAGTGAGAGGTAGGGCAGC
GCAAGTCAATCCTGGCGGATTCACTACCCCTGCGCGAAGGCCATCGGTGCCGCATCGAACGGCCGGTTGCGGAAAGTCCTCCCTGCGTCCGCTGATGGCC
GGCAGCAGCCCGTCGTTGCCTGATGGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGCAGCCGTCTTCTGAAAACGACAATGG
AGGTGGTAGCCGAGGGTGTGGAAACACCCGACTGCCTTGCGTGGTTGCGGCAGGCGGGTTGCGACACGGTGCAGGGTTTCCTGTTCGCCAGGCCGATGCC
GGCGGCGGCCTTCGTCGGCTTCGTCAACCAATGGAGGAACACCACCATGAACGCCAATGAACCGAGCACCAGTTGCTGCGTGTGCTGCAAGGAAATCCCG
CTCGATGCCGCCTTCACGCCGGAAGGGGCCGAGTACGTGGAGCATTTCTGCGGGCTGGAGTGCTATCAGCGCTTCCAGGCGCGGGCCAGCACTGCGACCG
AAACCAGCGTCAAACCGGACGCTTGTGATTCGCCGCCGTCAGGTTGAGGCATACCCTAACCTGATGTCAGATGCCATGTGTAAATTGCGTCAGGATAGGA
TTGAATTTTGAATTTATTGACATATCTCGTTGAAGGTCATAGAGTCTTCCCTGACATTTTGCAGGGAATTCCATGACTGGACAGCGCATTGGGTATATCA
GGGTCAGCACCTTCGACCAGAACCCGGAACGGCAACTGGAAGGCGTCAAGGTTGATCGCGCTTTTAGCGACAAGGCATCCGGCAAGGATGTCAAGCGTCC
GCAACTGGAAGCGCTGATAAGCTTCGCCCGCACCGGCGACACCGTGGTGGTGCATAGCATGGATCGCCTGGCGCGCAATCTCGATGATTTGCGCCGGATC
GTGCAAACGCTGACACAACGCGGCGTGCATATCGAATTCGTCAAGGAACACCTCAGTTTTACTGGCGAAGACTCTCCGATGGCGAACCTGATGCTCTCGG
TGATGGGCGCGTTCGCCGAGTTCGAGCGCGCCCTGATCCGCGAGCGTCAGCGCGAGGGTATTGCGCTCGCCAAGCAACGCGGGGCTTACCGTGGCAGGAA
GAAATCCCTGTCGTCTGAGCGTATTGCCGAACTGCGCCAACGTGTCGAGGCTGGCGAGCAAAAGACCAAGCTTGCTCGTGAATTCGGAATCAGTCGCGAA
ACCCTGTATCAATACTTGAGAACGGATCAGTAAATATGCCACGTCGTTCCATCCTGTCCGCCGCCGAGCGGGAAAGCCTGCTGGCGTTGCCGGACTCCAA
GGACGACCTGATCCGACATTACACATTCAACGATACCGACCTCTCGATCATCCGACAGCGGCGCGGGCCAGCCAATCGGCTGGGCTTCGCGGTGCAGCTC
TGTTACCTGCGCTTTCCCGGCGTCATCCTGGGCGTCGATGAACTACCGTTCCCGCCCTTGTTGAAGCTGGTCGCCGACCAGCTCAAGGTCGGCGTCGAAA
GCTGGAACGAGTACGGCCAGCGGGAGCAGACCCGGCGCGAGCACCTGAGCGAGCTGCAAACCGTGTTCGGTTTCCGGCCCTTCACCATGAGCCATTACCG
GCAGGCCGTCCAGATGCTGACCGAGCTGGCGATGCAAACCGACAAAGGCATCGTGCTGGCCAGCGCCTTGATCGGGCACCTGCGGCGGCAGTCGGTCATT
CTGCCCGCCCTCAACGCCGTCGAGCGGGCGAGTGCCGAGGCGATCACCCGTGCTAACCGGCGCATCTACGACGCCTTGGCCGAACCACTGGCGGACGCGC
ATCGCCGCCGCCTCGACGATCTGCTCAAGCGCCGGGACAACGGCAAGACGACCTGGTTGGCTTGGTTGCGCCAGTCTCCGGCCAAGCCAAATTCGCGGCA
TATGCTGGAACACATCGAACGCCTCAAGGCATGGCAGGCACTCGATCTGCCTACCGGCATCGAGCGGCTGGTTCACCAGAACCGCCTGCTCAAGATTGCC
CGCGAGGGCGGCCAGATGACACCCGCCGACCTGGCCAAATTCGAGCCGCAACGGCGCTACGCCACTCTCGTGGCGCTGGCCACCGAGGGCATGGCCACCG
TCACCGACGAAATCATCGACCTGCACGACCGCATCCTGGGTAAGCTGTTTAACGCTGCCAAGAATAAGCATCAGCAGCAGTTCCAGGCGTCAGGCAAGGC
CATCAACGCCAAGGTACGTCTGTACGGGCGCATCGGTCAGGCGCTGATCGACGCCAAGCAATCAGGCCGCGATGCGTTTGCCGCCATCGAGGCCGTCATG
TCCTGGGATTCCTTTGCCGAGAGCGTCACCGAGGCGCAGAAGCTCGCGCAACCCGATGACTTCGATTTCCTGCATCGCATCGGCGAGAGCTACGCCACCC
TGCGCCGCTATGCACCGGAATTCCTTGCCGTGCTCAAGCTGCGGGCCGCGCCCGCCGCCAAAAACGTGCTTGATGCCATTGAGGTGCTGCGCGGCATGAA
CACCGACAACGCCCGCAAGCTGCCAGCCGATGCACCGACCGGCTTCATCAAGCCGCGCTGGCAGAAACTGGTGATGACCGACGCCGGCATCGACCGGCGC
TACTACGAACTGTGCGCGCTGTCCGAGTTGAAGAACTCCCTGCGCTCGGGCGACATCTGGGTGCAGGGTTCACGCCAGTTCAAGGACTTCGAGGACTACC
TGGTACCGCCCGAGAAGTTCACCAGCCTCAAGCAGTCCAGCGAATTGCCGCTGGCCGTGGCCACCGACTGCGAACAATATCTGCATGAGCGGCTGACGCT
GCTGGAAGCACAACTTGCCACCGTCAACCGCATGGCGGCAGCCAACGACCTGCCGGATGCCATCATCACCGAGTCGGGCTTGAAGATCACGCCGCTGGAT
GCGGCGGTGCCCGACACCGCGCAGGCGCTGATAGACCAGACAGCCATGGTCCTGCCGCACGTCAAGATCACCGAACTGCTGCTCGAAGTCGATGAGTGGA
CGGGCTTCACCCGGCACTTCACGCACTTGAAATCGGGCGATCTGGCCAAGGACAAGAACCTGTTGTTGACCACGATCCTGGCCGACGCGATCAACCTGGG
CCTGACCAAGATGGCCGAGTCCTGCCCCGGCACGACCTACGCGAAGCTCGCTTGGCTGCAAGCCTGGCATACCCGCGACGAAACGTACTCGACAGCGTTG
GCTGAACTGGTCAACGCTCAGTTTCGGCATCCCTTTGCCGGGCACTGGGGCGATGGCACCACATCATCATCGGACGGACAGAATTTCCGAACCGCTAGCA
AGGCAAAGAGCACGGGGCACATCAACCCAAAATATGGCAGCAGCCCAGGACGGACTTTCTACACCCACATCTCCGACCAATACGCGCCATTCCACACCAA
GGTGGTCAATGTCGGCCTGCGCGACTCAACCTACGTGCTCGACGGCCTGCTGTACCACGAATCCGACCTGCGGATCGAGGAGCACTACACCGACACGGCG
GGCTTCACCGATCACGTCTTCGCCCTGATGCACCTCTTGGGCTTCCGCTTCGCGCCGCGCATCCGCGACCTGGGCGACACCAAGCTCTACATCCCGAAGG
GCGATGCCGCCTATGACGCGCTCAAGCCGATGATCGGCGGCACGCTCAACATCAAGCACGTCCGCGCCCATTGGGACGAAATCCTGCGGCTGGCCACCTC
GATCAAGCAGGGCACGGTGACGGCCTCGCTGATGCTCAGGAAACTCGGCAGCTACCCGCGCCAGAACGGCTTGGCCGTCGCGCTGCGCGAGTTGGGCCGC
ATCGAGCGCACGCTGTTCATCCTCGACTGGCTGCAAAGCGTCGAGCTACGCCGCCGCGTGCATGCCGGGCTGAACAAGGGCGAGGCGCGCAATGCGCTGG
CCCGTGCCGTGTTCTTCAACCGCCTTGGTGAAATCCGTGACCGCAGTTTCGAGCAGCAGCGCTACCGGGCCAGCGGCCTCAACCTGGTGACGGCGGCCAT
CGTGCTGTGGAACACGGTCTACCTGGAGCGTGCGGCGCATGCGTTGCGCGGCAATGGTCATGCCGTCGATGACTCGCTATTGCAGTACCTGTCGCCACTC
GGCTGGGAGCACATCAACCTGACCGGTGATTACCTATGGCGCAGCAGCGCCAAGATCGGCGCGGGGAAGTTCAGGCCGCTACGGCCTCTGCAACCGGCTT
AGCGTGCTTTATTTTCCGTTTTCTGAGACGACCCC
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
561 bp | 186 aa | 15173 | 15733 | + | No |
AG : Tn3 resolvase
ORF sequence :
MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGED
SPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQYLRTDQ
SPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQYLRTDQ
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
2967 bp | 988 aa | 15736 | 18702 | + | No |
Chemistry : DDE
ORF sequence :
MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRGPANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTR
REHLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALNAVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRR
DNGKTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALATEGMATVTDEIIDLHDRI
LGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVL
KLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQ
SSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKS
GDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKY
GSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMI
GGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA
REHLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALNAVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRR
DNGKTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALATEGMATVTDEIIDLHDRI
LGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVL
KLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQ
SSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKS
GDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKY
GSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMI
GGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA
Blast result :
Comments
TnAs3 is 97% (ORF22 : the transposase) and 100% (ORF21 : the resolvase) aa similar to ISShes11.
ORF1 to ORF19 are passenger gene.
ORF1 to ORF19 are passenger gene.
References
1] Friedhelm Pfeiffer (2015) Direct submission.
2] Reith,M.E., Singh,R.K., Curtis,B., Boyd,J., Bouevitch,A., Kimball,J., Munholland,J., Murphy,C., Sarty,D., Williams,J., Nash,J., Johnson,S. and Brown,L. (2007) Direct submission GenBank.
2] Reith,M.E., Singh,R.K., Curtis,B., Boyd,J., Bouevitch,A., Kimball,J., Munholland,J., Murphy,C., Sarty,D., Williams,J., Nash,J., Johnson,S. and Brown,L. (2007) Direct submission GenBank.