ISAeme25

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Aeromonas media
Aeromonas media
DNA section
IS Length : 1385 bp

Ends


IR Length : 20/26

IRL : GAGTTATGGTGATTTCTGGTGTATGGGGGATATAGAGAAGGTGGCGTATC
IRR : GAGCTATTGTCAAATGTGGTGTATGGAGTAGGTCAGGCGGCATTCCTGTC

Insertion site


Left flankDirect repeatRight flankDR Length
GAGAGTTTTGCAAGGCAAGAGCAGGATGC9

DNA sequence

GAGTTATGGTGATTTCTGGTGTATGGGGGATATAGAGAAGGTGGCGTATCCTGCTGATTGATTCCGCTCAAAGAATTCAATCAACAGAGGTGATACGCCA
TGACCGATTCTACCCTCAGAGCCCTGCCACAACCAGAGGCCAAGTCAACAGATCCGCTCACAGACCTGCTGCGTGCAGGAGCCAGAGACCTGATTGCCCA
GGCTGTGGAAGCAGAGCTTCAAACCCTGCTTGAGCGCCACGCTCAGCTTAAGCTCCCCGATGGCCGCCAAGCGTTGGTACGCAACGGCTTTTTGCCTGAA
CGCACCATCCAGACCGGGATCGGCGATGTCGAGATCAAGGTGCCAAAGGTGCGCGACAGAAGTGGTTCAGGGATCTGCTTCAACAGTTCGCTGCTGCCGC
CCTATCTCAAGCGTTCCAGCAGCATAGAAGAGCTGCTGCCCTGGCTGTACCTGCGGGGGATTTCCACTGGAGATTATCAGGAGGCGCTGGGCGCGTTGCT
CGGTGACAAGGCCAAGGGGTTGTCGGCCAATACCATCAGCCGCCTGAAACAGCACTGGGGTGATGAGCATCGGGAGTGGAGCCAGCGGGACCTGAGCCAG
AAGCGGTATGTGTACTTTTGGGCTGATGGGATATACAGCAACGTGCGCATGGGAGACCGGCTTTGCTTGCTGGTTATCATTGGCGTAACGGAGCATGGCC
ATAAGGAACTGGTGGCGGTCGAGGATGGCCTGAGGGAGTCTGAGACCAGCTGGACAGAGCTCCTGATGGGGCTGCGAGCCAGAGGGCTGGGCAAGGGCCC
CAAGCTTGCCGTCGGTGATGGCGCCCTGGGTTTTTGGCGAGCGCTGGCCAAGTGCTACCCGGATACGGTCCATCAACGATGTTGGGTTCACAAGACCGCG
AACGTGCTGGCAGCGTTGCCCAAATCTGTGCATCTCAAGGTCAAGGATGCGCTGCATGAAATCTGGATGGCCGAGACAAGGGATGAGGCTCACATGGCGT
TTGACCGCATTTTGGTGCGCTTTGAAGCGAAGTACCCGAAGGCGATGGAGAAGCTGACGAAAGACCGGGAAGAGCTGCTGGCGTTCTATGATTTTCCGGC
CGAGCACTGGGTGCATATCCGGACGACGAACCCGATAGAGTCGACGTTTTCGACGGTCAGGCTACGAAGCAAGAGAGCGCGGAACTGTGGGTCTCGGGAG
ACCACGCTGGCGATGGTTTACAAGCTGCTTGAAGCGGCACAGAAGAAGTGGAAGCGGCTCAAGGGATTTGTGCTGCTGACGTTGGTGGTAAACAACGTGA
AATTCAAGGACGGAGTCCAGGTGCTGGATGAGTCAGACAGGAATGCCGCCTGACCTACTCCATACACCACATTTGACAATAGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1254 bp417 aa1001353+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTDSTLRALPQPEAKSTDPLTDLLRAGARDLIAQAVEAELQTLLERHAQLKLPDGRQALVRNGFLPERTIQTGIGDVEIKVPKVRDRSGSGICFNSSLLP
PYLKRSSSIEELLPWLYLRGISTGDYQEALGALLGDKAKGLSANTISRLKQHWGDEHREWSQRDLSQKRYVYFWADGIYSNVRMGDRLCLLVIIGVTEHG
HKELVAVEDGLRESETSWTELLMGLRARGLGKGPKLAVGDGALGFWRALAKCYPDTVHQRCWVHKTANVLAALPKSVHLKVKDALHEIWMAETRDEAHMA
FDRILVRFEAKYPKAMEKLTKDREELLAFYDFPAEHWVHIRTTNPIESTFSTVRLRSKRARNCGSRETTLAMVYKLLEAAQKKWKRLKGFVLLTLVVNNV
KFKDGVQVLDESDRNAA

 

Blast result :
Comments
ISAeme25 is 84% aa similar to ISTdr1.
References
1] Pfeiffer F. (2015) Direct submission